Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,563,870 A→C 8.6% intergenic (+94/+28) PP_1372 → / ← pitB hypothetical protein/phosphate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,563,8700AC 8.6% 153.2 / 8.5 58intergenic (+94/+28)PP_1372/pitBhypothetical protein/phosphate transporter
Reads supporting (aligned to +/- strand):  ref base A (26/27);  new base C (3/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.81e-01

CGGCAGTTGGTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCA  >  NC_002947/1563723‑1564007
                                                                                                                                                   |                                                                                                                                         
cggcAGTTGGTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAgg                                                                                                                                         <  1:579536/150‑1 (MQ=255)
cggcAGTTGGTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAgg                                                                                                                                         <  1:1126234/150‑1 (MQ=255)
     gTTGGTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGAGCGcc                                                                                                                                    <  2:1318397/150‑1 (MQ=255)
         gTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTATCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGATGGCGCCttca                                                                                                                                <  1:249381/150‑1 (MQ=255)
              cgGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTtcattcat                                                                                                                           <  2:1246277/150‑1 (MQ=255)
                  ttGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCg                                                                                                                       <  2:212177/150‑1 (MQ=255)
                    gATGGGGTCGTTGTTAGGGGGCAGGAACAGGTAACCCTCGGGTGCCGCGCCATCCCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGgt                                                                                                                     <  2:951117/150‑1 (MQ=255)
                    gATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGgt                                                                                                                     <  2:344740/150‑1 (MQ=255)
                         ggTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCAc                                                                                                                >  1:319686/1‑150 (MQ=255)
                           tCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCACCTTCATTCATTGCGGTGTCACTc                                                                                                              >  1:1235419/1‑150 (MQ=255)
                              ttgttAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGc                                                                                                           >  1:910565/1‑150 (MQ=255)
                                 ttAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCaa                                                                                                        <  2:1235419/150‑1 (MQ=255)
                                  tAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAAt                                                                                                       <  1:742698/150‑1 (MQ=255)
                                      gggCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTtcattcat                                                                                                                           >  2:351240/1‑126 (MQ=255)
                                      gggCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTtcattcat                                                                                                                           <  1:351240/126‑1 (MQ=255)
                                       ggCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCtt                                                                                                  <  1:1461739/150‑1 (MQ=255)
                                           aGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTg                                                                                                >  1:30814/1‑148 (MQ=255)
                                           aGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTg                                                                                                <  2:30814/148‑1 (MQ=255)
                                                   tAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACgccagcc                                                                                      >  1:476900/1‑150 (MQ=255)
                                                        ttCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAAc                                                                                 >  2:1057814/1‑150 (MQ=255)
                                                          ccTGCGCCGCGCAATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAg                                                                               >  2:1593895/1‑150 (MQ=255)
                                                            tGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCTCGGGCGCCtttt                                                                                                                                <  1:1131025/99‑3 (MQ=255)
                                                            tGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCTCGGGCGCCtttt                                                                                                                                >  2:1131025/1‑97 (MQ=255)
                                                                           ccTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCa                                                              >  1:1458588/1‑150 (MQ=255)
                                                                           ccTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCa                                                              >  2:45188/1‑150 (MQ=255)
                                                                            cTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCAt                                                             <  2:319686/150‑1 (MQ=255)
                                                                             ttGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATc                                                            >  2:1182147/1‑150 (MQ=255)
                                                                               ggTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCCGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCAGGCCGCCAGGCCCATCGa                                                          <  2:742650/150‑1 (MQ=255)
                                                                                gTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGag                                                         <  1:725162/150‑1 (MQ=255)
                                                                                     acaAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGaggcagg                                                    >  2:1392883/1‑150 (MQ=255)
                                                                                         gCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGcaggcagg                                                <  1:1030881/150‑1 (MQ=255)
                                                                                          cccGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAggg                                               <  1:45188/150‑1 (MQ=255)
                                                                                                   gCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGAccc                                      >  2:1632922/1‑150 (MQ=255)
                                                                                                    cAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCa                                     >  1:250165/1‑150 (MQ=255)
                                                                                                    cAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCa                                     >  1:1443290/1‑150 (MQ=255)
                                                                                                     aaaGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCAt                                    <  2:910565/150‑1 (MQ=255)
                                                                                                         aaCAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCca                                >  1:1431135/1‑150 (MQ=255)
                                                                                                                tGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGaggc                                                       >  1:452069/1‑120 (MQ=255)
                                                                                                                tGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGaggc                                                       <  2:452069/120‑1 (MQ=255)
                                                                                                                tGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGAt                         <  1:1392883/150‑1 (MQ=255)
                                                                                                                 ggCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATg                        <  1:1057814/150‑1 (MQ=255)
                                                                                                                  gCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAggccggcc                                                                        >  1:552814/1‑101 (MQ=255)
                                                                                                                  gCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAggccggcc                                                                        <  2:552814/101‑1 (MQ=255)
                                                                                                                      aGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGtttt                   <  1:1182147/150‑1 (MQ=255)
                                                                                                                      aGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTAAGGACCCATGCCAGCAGGATGGtttt                   >  1:1235270/1‑150 (MQ=255)
                                                                                                                        gCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTca                 <  2:250165/150‑1 (MQ=255)
                                                                                                                        gCATAACGGCTATGAAAAAGGCGCCCAAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTca                 >  1:329912/1‑150 (MQ=255)
                                                                                                                            aaCGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGt             >  2:1490591/1‑150 (MQ=255)
                                                                                                                                      atgaaGGCGCCCTCGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGc   >  2:1369219/4‑150 (MQ=255)
                                                                                                                                      aaaaaGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAAGAGGCCGGGCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGc   >  1:1349459/1‑150 (MQ=255)
                                                                                                                                       aaaaGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCa  >  1:1538477/1‑150 (MQ=255)
                                                                                                                                       aaaaGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATAGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCa  >  1:1196520/1‑150 (MQ=255)
                                                                                                                                        gaaGGCGCCCTCGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAg                                                                               <  1:135401/71‑1 (MQ=39)
                                                                                                                                        gaaGGCGCCCTCGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAg                                                                               >  2:135401/2‑72 (MQ=39)
                                                                                                                                            gcgcCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGAc                                                                                             <  2:417459/54‑1 (MQ=255)
                                                                                                                                            gcgcCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGAc                                                                                             >  1:417459/1‑54 (MQ=255)
                                                                                                                                              gcCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAAc                                                                                 >  1:212642/1‑64 (MQ=255)
                                                                                                                                              gcCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAAc                                                                                 <  2:212642/64‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
CGGCAGTTGGTGCGCGGGTTGATGGGGTCGTTGTTAGGGGGCAAGAAAAGGTAGTCTTCCTGCGCCGCGCCATACCCTTGGTAGCACAAGCCCGCTCCGGCAAAGAACAAGGTGGCGTAGGCATAACGGCTATGAAAAAGGCGCCCGAGGGCGCCTTCATTCATTGCGGTGTCACTCAGCCAATGGCTTTGGACGCCAGCCAGAACAGGCCGGCCGCCAGGCCCATCGAGGCAGGCAGGGTCAGGACCCATGCCAGCAGGATGGTTTTCACAGTGCCGCCTTGCA  >  NC_002947/1563723‑1564007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑