Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,608,967 A→T 5.6% L447Q (CTG→CAG)  PP_1411 ← GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,608,9670AT 5.6% 212.6 / 3.2 71L447Q (CTG→CAG) PP_1411GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (36/31);  new base T (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

TTGTCATTCATGGCGGGTTTCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTCCTCACCGCCCCACCGGCACAGTATATCTGACTGACGCAG  >  NC_002947/1608822‑1609115
                                                                                                                                                 |                                                                                                                                                    
ttGTCATTCATGGCGGGTTTCACTGCAGACACGGTTGCGGCCAGCCTGCGTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCgg                                                                                                                                                  >  2:640097/1‑150 (MQ=255)
     attcatGGCGGGTTTCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGt                                                                                                                                             >  2:1055142/1‑150 (MQ=255)
         atGGCGGGTTTCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTgg                                                                                                                                              >  2:1467183/1‑145 (MQ=255)
         atGGCGGGTTTCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTgg                                                                                                                                              <  1:1467183/145‑1 (MQ=255)
         atGGCGGGTTTCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTc                                                                                                                                         <  2:905903/150‑1 (MQ=255)
         atGGCGGGTTTCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTc                                                                                                                                         <  2:1603861/150‑1 (MQ=255)
         atGGCGGGTTTCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTc                                                                                                                                         >  1:4609/1‑150 (MQ=255)
            gCGGGTTTCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCTTGTAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTa                                                                                                                                      >  1:197691/1‑150 (MQ=255)
             cGGGTTTCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTaa                                                                                                                                     <  1:721022/150‑1 (MQ=255)
              gggTTTCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAAc                                                                                                                                    <  1:640097/150‑1 (MQ=255)
                 tttCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGa                                                                                                                                 >  2:1608597/1‑150 (MQ=255)
                   tCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAg                                                                                                                               >  2:77788/1‑150 (MQ=255)
                    cACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAgg                                                                                                                              >  2:432315/1‑150 (MQ=255)
                    cACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAgg                                                                                                                              <  1:1355008/150‑1 (MQ=255)
                    cACTGCAGACACGGTTGCGGCCAGCCTGCTGGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAgg                                                                                                                              <  1:252010/150‑1 (MQ=255)
                      cTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTc                                                                                                                            <  2:306330/150‑1 (MQ=255)
                       tGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCa                                                                                                                           >  1:404076/1‑150 (MQ=255)
                       tGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCa                                                                                                                           >  2:646891/1‑150 (MQ=255)
                        gCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAg                                                                                                                          <  1:1608597/150‑1 (MQ=255)
                            acacGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAgctgc                                                                                                                      >  1:477722/1‑150 (MQ=255)
                             cacGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAgctgct                                                                                                                     <  2:477722/150‑1 (MQ=255)
                                      ggCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTc                                                                                                            >  1:1461372/1‑150 (MQ=255)
                                        ccAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCTGCg                                                                                                                                                   <  1:1369140/109‑1 (MQ=255)
                                        ccAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCTGCg                                                                                                                                                   >  2:1369140/1‑109 (MQ=255)
                                            ccTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGa                                                                                                      >  1:532262/1‑150 (MQ=255)
                                             cTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGAt                                                                                                     >  2:86252/1‑150 (MQ=255)
                                              tGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAgg                                                                                                                              <  2:1449529/124‑1 (MQ=255)
                                              tGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAgg                                                                                                                              >  1:1449529/1‑124 (MQ=255)
                                                 ttGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGAttttt                                                                                                 >  1:461918/1‑150 (MQ=255)
                                                 ttGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGAttttt                                                                                                 >  2:1401644/1‑150 (MQ=255)
                                                       cGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGcc                                                                                           >  1:883369/1‑150 (MQ=255)
                                                         aTAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCAc                                                                                         >  1:817299/1‑150 (MQ=255)
                                                          tAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCAcc                                                                                        >  1:1357404/1‑150 (MQ=255)
                                                          tAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCAcc                                                                                        >  1:775627/1‑150 (MQ=255)
                                                           aaaGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCt                                                                                       <  1:1401644/150‑1 (MQ=255)
                                                           aaaGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCCGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCt                                                                                       <  1:239041/150‑1 (MQ=255)
                                                                gcgcgGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATg                                                                                  <  2:883369/150‑1 (MQ=255)
                                                                    ggTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCg                                                                                                                                        >  2:587590/1‑92 (MQ=255)
                                                                    ggTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCg                                                                                                                                        <  1:587590/92‑1 (MQ=255)
                                                                           gcgGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGcc                                                                       <  2:461918/150‑1 (MQ=255)
                                                                                    cagGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTc                                                              <  2:1318367/150‑1 (MQ=255)
                                                                                              aGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTc                                                                                                                                         >  2:661665/1‑65 (MQ=255)
                                                                                              aGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTc                                                                                                                                         <  1:661665/65‑1 (MQ=255)
                                                                                               gggTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCgcagcagca                                                   >  1:452155/1‑150 (MQ=255)
                                                                                                     cccccGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGa                                             <  1:646891/150‑1 (MQ=255)
                                                                                                            ttGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTCCTc                                      <  2:775627/150‑1 (MQ=255)
                                                                                                             tGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTCCTCa                                     <  2:404076/150‑1 (MQ=255)
                                                                                                                aGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAAtt                                          <  2:1577432/142‑1 (MQ=255)
                                                                                                                aGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAAtt                                          >  1:1577432/1‑142 (MQ=255)
                                                                                                                  gctgctCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTgg                                                                            <  2:129977/106‑1 (MQ=255)
                                                                                                                  gctgctCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTgg                                                                            >  1:129977/1‑106 (MQ=255)
                                                                                                                       tCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGc                                                                                            <  1:1240857/85‑1 (MQ=255)
                                                                                                                       tCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGc                                                                                            >  2:1240857/1‑85 (MQ=255)
                                                                                                                       tCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTc                                         <  2:202001/136‑1 (MQ=255)
                                                                                                                       tCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTc                                         >  1:202001/1‑136 (MQ=255)
                                                                                                                         aGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCTGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTCCTCACCGCCCCACCgg                         >  1:934301/1‑150 (MQ=255)
                                                                                                                           gCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTc                                         <  2:494659/132‑1 (MQ=255)
                                                                                                                           gCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTc                                         >  1:494659/1‑132 (MQ=255)
                                                                                                                           gCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTCCTCACCGCCCCACCGGcc                       >  2:445597/1‑149 (MQ=255)
                                                                                                                           gCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTCCTCACCGCCCCACCGGca                       >  1:1489213/1‑150 (MQ=255)
                                                                                                                           gCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATCTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTCCTCACCGCCCCACCGGca                       >  1:242692/1‑150 (MQ=255)
                                                                                                                                    ggtggtCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTCCTCACCGCCCCACCGGCACAGTATATc              <  2:242692/150‑1 (MQ=255)
                                                                                                                                    gacaaccAGCCGCTGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCACAGCAGCACGATGAATTCCTCACCGCCCCACCGGca                       <  1:1002481/135‑1 (MQ=255)
                                                                                                                                    gacaaccAGCCGCTGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCACAGCAGCACGATGAATTCCTCACCGCCCCACCGGca                       >  2:1002481/7‑141 (MQ=255)
                                                                                                                                      tggtCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTc                                                                    >  2:1318963/1‑94 (MQ=255)
                                                                                                                                      tggtCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTc                                                                    <  1:1318963/94‑1 (MQ=255)
                                                                                                                                       ggtCAGCCGCAGCGGATGGTTGTCGTAACTGAACGTCAGCTGATCGGTGCGTCGGCGGATTTTTTCTGCCGCCTCAATGGCCTGGCGCCCTTCAGCTTCGCGCAGTAGCACGTTGAATTCCTCACCGCCACACCGGCACAGTATATctga           <  2:666091/150‑1 (MQ=255)
                                                                                                                                         tCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTCCTCACCGCCCCACCGGCACAGTATATctgact         >  2:577635/1‑150 (MQ=255)
                                                                                                                                              cGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTcc                                        <  1:276386/114‑1 (MQ=255)
                                                                                                                                              cGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTcc                                        >  2:276386/1‑114 (MQ=255)
                                                                                                                                                cAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTCCTCACCGCCCCACCGGCACAGTATATCTGACTGACGCAg  <  2:4609/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTGTCATTCATGGCGGGTTTCACTGCAGACACGGTTGCGGCCAGCCTGCTTGGCACGATAAAGCGCGCGGTCGGCGCGGGTCAGCAGGGCGTGCAGGGTATCCCCCGGTTGCAGGCTGCTCAGGCCGATGCTGGTGGTCAGCCGCAGCGGCTGGTTGTCGTAACTGAAGGTCAGCTGCTCGGTGCGTCGGCGGATTTTTTCTGCCACCTCGATGGCCTGGCGGCCTTCAGCTTCGCGCAGCAGCACGATGAATTCCTCACCGCCCCACCGGCACAGTATATCTGACTGACGCAG  >  NC_002947/1608822‑1609115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑