Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,695,552 G→C 8.8% V63L (GTG→CTG)  PP_1491 → CheW domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,695,5520GC 8.8% 173.6 / 8.3 57V63L (GTG→CTG) PP_1491CheW domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (25/27);  new base C (3/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

CTGCTGGAACCGCATTGGCGTGCGCGGCGACAAGCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGCATTCATTGCCGCACCAGCGCTCGCGGCTGCT  >  NC_002947/1695404‑1695697
                                                                                                                                                    |                                                                                                                                                 
ctgctgGAACCGCATTGGCGTGCGCGGCGACAAGCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGt                                                                                                                                                  <  1:1596554/150‑1 (MQ=255)
   ctgGAACCGCATTGGCGTGCGCGGCGACAAGCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCa                                                                                                                                               <  2:1214925/150‑1 (MQ=255)
   ctgGAACCGCATTGGCGTGCGCGGCGACAAGCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCa                                                                                                                                               <  2:663781/150‑1 (MQ=255)
   ctgGAACCGCATTGGCGTGCGCGGCGACAAGCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGACACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCa                                                                                                                                               >  1:1344517/1‑150 (MQ=255)
    tgGAACCGCATTGGCGTGCGCGGCGACAAGCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGt                                                                                                                                                  <  1:790128/146‑1 (MQ=255)
    tgGAACCGCATTGGCGTGCGCGGCGACAAGCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGt                                                                                                                                                  >  2:790128/1‑146 (MQ=255)
            cATTGGCGTGCGCGGCGACAAGCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGc                                                                                                                                      >  1:492275/1‑150 (MQ=255)
            cATTGGCGTGCGCGGCGACAAGCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGc                                                                                                                                      >  2:903438/1‑150 (MQ=255)
                            gACAAGCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAACTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGt                                                                                                                      <  2:1183446/150‑1 (MQ=255)
                            gACAAGCATTGCCCACTGCTGAAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCCACGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGAAGAGGTGCAGCCGGATGCGGAAAGCACCGGTctt                                                                                                                      >  2:157109/1‑148 (MQ=255)
                               aaGCATTGCCCACTGCTGGCGCGGCATGTTCGTTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCg                                                                                                                   >  2:1015143/1‑150 (MQ=255)
                                 gCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGAt                                                                                                                 <  2:891653/150‑1 (MQ=255)
                                  cATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGAtg                                                                                                                >  2:390601/1‑150 (MQ=255)
                                  cATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGAtg                                                                                                                >  1:166619/1‑150 (MQ=255)
                                   aTTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGAtgc                                                                                                               >  1:374246/1‑150 (MQ=255)
                                    ttGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGAtgct                                                                                                              <  2:374246/150‑1 (MQ=255)
                                     tGCCCACTGCTGGAGCGGCATGGTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGACACGCGTTTGCTCCACCGCTATGCGCTGGTTCAGTTGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGAtgctg                                                                                                             <  1:903438/150‑1 (MQ=255)
                                         cACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGt                                                                                                         <  2:492275/150‑1 (MQ=255)
                                          aCTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGtt                                                                                                        <  2:1205420/150‑1 (MQ=255)
                                            tgctgGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTcc                                                                                                      <  2:1344517/150‑1 (MQ=255)
                                                 aaGCGGCATGTCCTTTGCGCCAATTGGGAGTTGTATGCCGCGCGGGCCACGCGTTTCCTCGACCCCTATGCGCTGGTGAAGACGAACCAGGCGGCAGCGGTGCAGCGGGATGGGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTg                                                                                                 <  1:1143691/149‑1 (MQ=255)
                                                           ttCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCg                                                                                                                           <  1:455505/114‑1 (MQ=255)
                                                           ttCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCg                                                                                                                           >  2:455505/1‑114 (MQ=255)
                                                           ttCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGaa                                                                                           >  2:201608/1‑146 (MQ=255)
                                                           ttCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGaa                                                                                           <  1:201608/146‑1 (MQ=255)
                                                              aTTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGtggc                                                                                    >  1:554504/1‑150 (MQ=255)
                                                                  ccGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGtggctggc                                                                                >  1:1010445/1‑150 (MQ=255)
                                                                     cAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACCCCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGgctggcgct                                                                             <  1:1232912/150‑1 (MQ=255)
                                                                     cAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGgctggcgct                                                                             <  2:554504/150‑1 (MQ=255)
                                                                       aTTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGgctggcgctgg                                                                           <  1:95057/150‑1 (MQ=255)
                                                                        ttGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGgctggcgctggc                                                                          <  2:223398/150‑1 (MQ=255)
                                                                               gtgtATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCgcc                                                                   >  2:668241/1‑150 (MQ=255)
                                                                                  tATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCgcctgc                                                                <  1:390601/150‑1 (MQ=255)
                                                                                      ccGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCgcctg                                                                 >  1:1395973/1‑145 (MQ=255)
                                                                                      ccGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCgcctg                                                                 <  2:1395973/145‑1 (MQ=255)
                                                                                             ggCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAAt                                                     >  1:456021/1‑150 (MQ=255)
                                                                                                     gTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGt                                             >  1:298995/1‑150 (MQ=255)
                                                                                                         gCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTc                                                                                                   <  2:312686/92‑1 (MQ=255)
                                                                                                         gCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTc                                                                                                   >  1:312686/1‑92 (MQ=255)
                                                                                                          cTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTATCGCCTGCCTGGCCGAAATCGCCCCGTTGCAg                                        <  2:1010445/150‑1 (MQ=255)
                                                                                                           tCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCgcctgccac                                                             <  1:1361542/128‑3 (MQ=255)
                                                                                                           tCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCgcctgccac                                                             >  2:1361542/1‑126 (MQ=255)
                                                                                                           tCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGAGGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAgg                                       >  1:298323/1‑150 (MQ=255)
                                                                                                              aCCGCTATGCGCTGGTGCAGGAGCACCCGGCTGCACCGCTGCAGcc                                                                                                                                            >  1:1146583/1‑46 (MQ=21)
                                                                                                              aCCGCTATGCGCTGGTGCAGGAGCACCCGGCTGCACCGCTGCAGcc                                                                                                                                            <  2:1146583/46‑1 (MQ=21)
                                                                                                                cGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTg                                  >  2:735828/1‑150 (MQ=255)
                                                                                                                cGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTg                                  >  1:545189/1‑150 (MQ=255)
                                                                                                                   tATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGcat                               >  2:959383/1‑150 (MQ=255)
                                                                                                                         cTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGCATTCATTg                         <  1:1452170/150‑1 (MQ=255)
                                                                                                                          tGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGCATTCATTGc                        >  2:233827/1‑150 (MQ=255)
                                                                                                                           ggTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGATCGCCATCTCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGCATTCATTGcc                       <  1:1350459/150‑1 (MQ=255)
                                                                                                                               cAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGCATTCATTGACGCAc                   >  2:60149/1‑150 (MQ=255)
                                                                                                                                 ggAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGTGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGCATTCATTGCCGCACCa                 <  2:545189/150‑1 (MQ=255)
                                                                                                                                    gcatccggCTGCACCGCTGCAGCCTGGTGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGAGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGCATTCATTACCGCACCAgcg              >  2:306956/7‑150 (MQ=255)
                                                                                                                                         cggCTGCACCGCTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGCATTCATTGCCGCACCAgc               >  1:1559321/2‑144 (MQ=255)
                                                                                                                                         cggCTGCACCGCTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGCATTCATTGCCGCACCAgc               <  2:1559321/143‑1 (MQ=255)
                                                                                                                                                aGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGCATTCATTGCCGCACCAGCGCTCGCGgctgct  <  1:233827/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CTGCTGGAACCGCATTGGCGTGCGCGGCGACAAGCATTGCCCACTGCTGGAGCGGCATGTTCATTGCCGCAATTGCGAGGTGTATGCCGCTGCGGCCACGCGTTTGCTCGACCGCTATGCGCTGGTGCAGGAGCACCAGGCTGCAGCGGTGCAGCCGGATGCGGAAAACACCGGTCGTTCGATGCTGCTGTTCCGTCTGGGCGAAGAGTGGCTGGCGCTGGCTACCGCCTGCCTGGCCGAAATCGCCCCGTTGCAGGCGGTGCATTCATTGCCGCACCAGCGCTCGCGGCTGCT  >  NC_002947/1695404‑1695697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑