Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,810,377	T→G	7.6%	intergenic (+96/‑73)	eno → / → ftsB	enolase/cell division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,810,377	0	T	G	7.6%	176.5 / 6.5	66	intergenic (+96/‑73)	eno/ftsB	enolase/cell division protein
Reads supporting (aligned to +/- strand):  ref base T (28/33);  new base G (3/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

GCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGTGGGTGGGT  >  NC_002947/1810240‑1810524                                                                                                                                           |                                                                                                                                                    gcCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCTGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTc                                                                                                                                         <  1:1324760/150‑1 (MQ=255)     aaGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGc                                                                                                                                     >  2:763713/1‑150 (MQ=255)                cgcgGTCGTGCCGAGTTTCGCGGCTAAGAAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACTAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGcc                                                                                                                          <  1:107150/150‑1 (MQ=255)                     tCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGaa                                                                                                                     <  2:491472/150‑1 (MQ=255)                        tGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAAGCCCCACAGTa                                                                                                                  >  2:588095/1‑150 (MQ=255)                          ccGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAAc                                                                                                                <  1:263611/150‑1 (MQ=255)                            gAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACaa                                                                                                              >  1:1528119/1‑150 (MQ=255)                                           gCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCtt                                                                                               <  1:296937/150‑1 (MQ=255)                                             aaGAGATGGTAAAAATGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCtttt                                                                                             <  1:171399/150‑1 (MQ=255)                                               gagaTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATCCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTa                                                                                           >  1:823123/1‑150 (MQ=255)                                                  aTGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAAcc                                                                                        <  1:725891/150‑1 (MQ=255)                                                    ggTAAAAAGGCAGCAGCCGGAGTTGTAGGAACG‑TCGAAGAGGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTg                                                                                      <  1:1319417/150‑1 (MQ=255)                                                        aaaaGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAg                                                                                                             >  2:1528219/1‑123 (MQ=255)                                                        aaaaGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAg                                                                                                             <  1:1528219/123‑1 (MQ=255)                                                                   gcCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATgcgc                                                                       >  2:1489151/1‑150 (MQ=255)                                                                       gAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGc                                                                                                                                                 <  1:236742/72‑1 (MQ=255)                                                                       gAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGc                                                                                                                                                 >  2:236742/1‑72 (MQ=255)                                                                         gTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTccc                                                                 >  2:309226/1‑150 (MQ=255)                                                                             tAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTAtt                                                             <  2:1528119/150‑1 (MQ=255)                                                                               ggAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATCCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTgg                                                           <  2:823123/150‑1 (MQ=255)                                                                                gAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGccc                                                                                                                         >  1:1617431/1‑87 (MQ=255)                                                                                gAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGccc                                                                                                                         <  2:1617431/87‑1 (MQ=255)                                                                                 aaCGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgtt                                                         <  2:1466185/150‑1 (MQ=255)                                                                                    gTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGt                                                                                                                                       >  1:1320230/1‑69 (MQ=255)                                                                                    gTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGt                                                                                                                                       <  2:1320230/69‑1 (MQ=255)                                                                                    gTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgttgtt                                                      >  2:410892/1‑150 (MQ=255)                                                                                    gTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAAAAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgttgtt                                                      >  2:1041670/1‑150 (MQ=255)                                                                                        gTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTTTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTc                                                  <  1:588095/150‑1 (MQ=255)                                                                                            cTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGtt                                               >  1:117153/1‑150 (MQ=255)                                                                                             tGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGcc                                                                                                                          <  1:708949/74‑1 (MQ=255)                                                                                             tGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGcc                                                                                                                          >  2:708949/1‑74 (MQ=255)                                                                                              gAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTc                                                     >  1:361395/1‑142 (MQ=255)                                                                                              gAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTc                                                     <  2:361395/142‑1 (MQ=255)                                                                                               aaatttCCTACCTACTAACGGGTTTCTGTCGACAAAGCCTGGCCTCCGCCAGGCTTCGTGCTATTCGAGGCCAGTAAGTAATCAGTACGCGGCAGCATTTTTAAACCGGATAGCGTTATGCGCAGACTCTATTCGTTGGTTCTCGTTCTg                                            <  2:626752/147‑1 (MQ=255)                                                                                                  tttCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGctc                                         >  2:1238374/1‑150 (MQ=255)                                                                                                       tACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCACGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCt                                    >  1:988890/1‑150 (MQ=255)                                                                                                         cGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGt                                  <  2:117153/150‑1 (MQ=255)                                                                                                          gTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGtt                                 <  1:1238374/150‑1 (MQ=255)                                                                                                            aCTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTg                                                            <  2:1502246/121‑1 (MQ=255)                                                                                                            aCTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTg                                                            >  1:1502246/1‑121 (MQ=255)                                                                                                               aaCGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTg                            >  1:355760/1‑150 (MQ=255)                                                                                                                aCGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTgg                           >  2:280604/1‑150 (MQ=255)                                                                                                                aCGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCGGTATAACGTGATGCGCAGTCCCTATGGGTGTTTGCTCTGTCTGCTCTTTCTGTTGAGTgg                           >  2:1577925/1‑150 (MQ=255)                                                                                                                  gggTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTg                                <  1:79273/143‑1 (MQ=255)                                                                                                                  gggTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTg                                >  2:79273/1‑143 (MQ=255)                                                                                                                    gTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgttgtt                                                      <  1:1517381/119‑1 (MQ=255)                                                                                                                    gTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgttgtt                                                      >  2:1517381/1‑119 (MQ=255)                                                                                                                         tGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTa                  <  1:280604/150‑1 (MQ=255)                                                                                                                              acgaAGCCTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCt                                                                                       >  1:291401/1‑76 (MQ=255)                                                                                                                              acgaAGCCTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCt                                                                                       <  2:291401/76‑1 (MQ=38)                                                                                                                              acgaAGCCTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGctct                                        <  1:389320/123‑1 (MQ=255)                                                                                                                              acgaAGCCTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGctct                                        >  2:389320/1‑123 (MQ=255)                                                                                                                               cgaAGCCTGGCCGAGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCt            >  2:926343/1‑150 (MQ=255)                                                                                                                                gaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgttgt                                                       >  2:1627254/1‑106 (MQ=255)                                                                                                                                gaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGgttgt                                                       <  1:1627254/106‑1 (MQ=255)                                                                                                                                gaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTg                                >  2:733359/1‑129 (MQ=255)                                                                                                                                gaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTg                                <  1:733359/129‑1 (MQ=255)                                                                                                                                gaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGt                             <  2:307627/132‑1 (MQ=255)                                                                                                                                gaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGt                             >  1:307627/1‑132 (MQ=255)                                                                                                                                gaAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCtg           >  2:1405207/1‑150 (MQ=255)                                                                                                                                   gCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTgtgg        >  1:1470692/1‑150 (MQ=255)                                                                                                                                     cTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTgtgggt      <  1:1577925/150‑1 (MQ=255)                                                                                                                                     cTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTATTGGTCCTGCAGTACCGCCTgtgggt      <  1:1041670/150‑1 (MQ=255)                                                                                                                                      tGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTgtgggtg     <  2:355760/150‑1 (MQ=255)                                                                                                                                       ggCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTgtgggtgg    <  1:1405207/150‑1 (MQ=255)                                                                                                                                         ccTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGTgggtgggt  <  1:410892/150‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                    GCCAAAGCGGTTTACCGCGGTCGTGCCGAGTTTCGCGGCTAAGCAAGAGATGGTAAAAAGGCAGCAGCCGGAGTTGTAGGAACGTTCGTA‑CTGAACTTTCCTACGTACTAACGGGTTTCTGTCGACGAAGCCTGGCCTCGGCCAGGCTTCGTGCTATTCGAGGCCCCGAAGTAACAAGTACGCGGCAGCCTTTTTAACCTGGATAACGTGATGCGCAGTCCCTATTGGTTGTTCCTCGTTCTGCTCTTGCTGTTGGGTGGCCTGCAGTACCGCCTGTGGGTGGGT  >  NC_002947/1810240‑1810524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑