Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,831,822	T→C	6.3%	L220P (CTG→CCG)	yfiP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,831,822	0	T	C	6.3%	186.9 / 2.5	63	L220P (CTG→CCG)	yfiP	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/30);  new base C (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.37e-01

TGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTGACCGT  >  NC_002947/1831675‑1831971                                                                                                                                                    |                                                                                                                                                      tGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGc                                                                                                                                                     <  1:319874/150‑1 (MQ=255)        cTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGa                                                                                                                                              <  1:778127/150‑1 (MQ=255)         tCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGAc                                                                                                                                             <  1:1187695/150‑1 (MQ=255)         tCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGAc                                                                                                                                             >  1:912134/1‑150 (MQ=255)           cAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGa                                                                                                                                           >  2:782604/1‑150 (MQ=255)              gcgcGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCa                                                                                                                                        <  1:388562/150‑1 (MQ=255)               cgcgATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAg                                                                                                                                       <  1:782604/150‑1 (MQ=255)                  gatgatCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGgac                                                                                                                                    >  1:371026/1‑150 (MQ=255)                         aTTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGAcaccc                                                                                                                             >  1:339474/1‑150 (MQ=255)                          tttCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGAcaccca                                                                                                                            >  1:1065747/1‑150 (MQ=255)                              tGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCAGCCGCCGCTTgcc                                                                                                                                             <  2:1284636/129‑3 (MQ=255)                              tGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCAGCCGCCGCTTgcc                                                                                                                                             >  1:1284636/1‑127 (MQ=255)                               gTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCGGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGTCTGCCGCTTGACGAGCAGGACGACACCCACCAGc                                                                                                                       >  1:379555/1‑150 (MQ=255)                                tACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCg                                                                                                                      <  1:258849/150‑1 (MQ=255)                                   ggCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGttt                                                                                                                   <  2:553397/150‑1 (MQ=255)                                      aGAAGTTGCGGAGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCa                                                                                                                <  2:644457/150‑1 (MQ=255)                                         aGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATAc                                                                                                             <  1:1594963/150‑1 (MQ=255)                                               ggcgATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCt                                                                                                       >  1:316239/1‑150 (MQ=255)                                                      tgCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGTCAAGCGGCAGCCGCCGCTTgcc                                                                                                                                             <  1:977227/105‑3 (MQ=255)                                                      tgCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGTCAAGCGGCAGCCGCCGCTTgcc                                                                                                                                             >  2:977227/1‑103 (MQ=255)                                                        ccTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTc                                                                                                         >  1:552731/1‑139 (MQ=255)                                                        ccTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTc                                                                                                         <  2:552731/139‑1 (MQ=255)                                                           ggACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAggg                                                                                           >  1:106070/1‑150 (MQ=255)                                                            gACCTTGCTGGCGATGGCCAGGCTTTGCAGCCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGTCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAgggg                                                                                          <  2:379555/150‑1 (MQ=255)                                                                ttGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGcaca                                                                                      >  2:369462/1‑150 (MQ=255)                                                                            gCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTg                                                                          <  2:106070/150‑1 (MQ=255)                                                                             ccAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCtt                                                                            >  1:1014330/1‑147 (MQ=255)                                                                             ccAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCtt                                                                            <  2:1014330/147‑1 (MQ=255)                                                                              cAGGCTTCGCAGGCGCTGGATGCCTATCAGGATGTGTTCAGCCAGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGt                                                                        >  1:1520716/1‑150 (MQ=255)                                                                                 gCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCa                                                                     >  2:541209/1‑150 (MQ=255)                                                                                      gCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTc                                                                      >  2:659674/1‑144 (MQ=255)                                                                                      gCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTc                                                                      <  1:659674/144‑1 (MQ=255)                                                                                        aGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTg                                                                               >  2:1499234/1‑133 (MQ=255)                                                                                        aGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTg                                                                               <  1:1499234/133‑1 (MQ=255)                                                                                          gcgcTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACgg                                                            <  2:339474/150‑1 (MQ=255)                                                                                            gcTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTgg                                                                         >  2:278462/1‑135 (MQ=255)                                                                                            gcTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTgg                                                                         <  1:278462/135‑1 (MQ=255)                                                                                                     ttATTTGGATGTGTTTTGCGTTTATTATCTTAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGcc                                                 <  2:912134/149‑1 (MQ=255)                                                                                                     cTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGcc                                                 >  2:1097136/1‑150 (MQ=255)                                                                                                     cTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGcc                                                 >  2:367196/1‑150 (MQ=255)                                                                                                          tGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACGACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGt                                            <  2:371026/150‑1 (MQ=255)                                                                                                          tGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCa                                                    >  1:319492/1‑142 (MQ=255)                                                                                                          tGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCa                                                    <  2:319492/142‑1 (MQ=255)                                                                                                            gATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTgg                                          >  2:1240586/1‑150 (MQ=255)                                                                                                            gATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTgg                                          >  2:365919/1‑150 (MQ=255)                                                                                                                tgtTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGc                                                                              >  1:65156/1‑110 (MQ=255)                                                                                                                tgtTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGc                                                                              <  2:65156/110‑1 (MQ=255)                                                                                                                  ttCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCgaga                                    >  2:127859/1‑150 (MQ=255)                                                                                                                  ttCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCgaga                                    >  1:169329/1‑150 (MQ=255)                                                                                                                  ttCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCgaga                                    <  2:1065747/150‑1 (MQ=255)                                                                                                                   tCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGAc                                   <  2:316239/150‑1 (MQ=255)                                                                                                                      gCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGc                                >  1:1372678/1‑150 (MQ=255)                                                                                                                        cTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGCCACCCACCAGCGTTCGCATACCTTCCTCTAGTCCAAGGGGCACAACCCCTGCTTCGGTTCATAATGACGGAGCTGGCAGCCAGGGGTGGCGAGACGGCAg                              >  1:586108/1‑150 (MQ=255)                                                                                                                         tGCATTACCTGCACGGCAAGCGGATGCTCCCTCTTGACTAGCCCGACGACACCCACCATCGTTTGCATACCTTCCTATAGTCCAAGGGGCACACCCCCTCCTTTGGTTCATAATAACGGAGCTTGCAGCCAGGGTGGGCGAGACGGCAgg                             <  1:312066/150‑1 (MQ=255)                                                                                                                           cATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGc                           <  2:1075235/150‑1 (MQ=255)                                                                                                                            aTTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCg                          <  2:1372678/150‑1 (MQ=255)                                                                                                                                  tGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAg                                                          <  2:1551393/112‑1 (MQ=255)                                                                                                                                  tGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAg                                                          >  1:1551393/1‑112 (MQ=255)                                                                                                                                   gAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCt                   <  1:369462/150‑1 (MQ=255)                                                                                                                                      cGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGa                <  2:1520716/150‑1 (MQ=255)                                                                                                                                           tgcggcgGCTGCCGCTGGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACTCCCCCGTCTTTGGTTCATAATGACTGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTcc           <  1:367196/149‑1 (MQ=255)                                                                                                                                               gcggcTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTg       >  1:831198/1‑150 (MQ=255)                                                                                                                                                 ggcTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTGAc     >  1:642476/1‑150 (MQ=255)                                                                                                                                                    tGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTGACCGt  >  1:569254/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                      TGCGGCGCTCCAAGCGCGATGATCATTTCTGTACGGCAGAAGTTGCGGCGATGTGCCTGGACCTTGCTGGCGATGGCCAGGCTTCGCAGGCGCTGGATGCCTATCTGGATGTGTTCAGCCTGCATTACCTGAGCGGCAAGCGGCGGCTGCCGCTTGACGAGCAGGACGACACCCACCAGCGTTTGCATACCTTCCTATAGTCCAAGGGGCACAACCCCTGCTTTGGTTCATAATGACGGAGCTGGCAGCCAGGGTGGGCGAGACGGCAGGGCGATTGGCTTGATCTCCCTTGACCGT  >  NC_002947/1831675‑1831971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑