| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,872,685 | G→A | 9.9% | G310S (GGT→AGT) | cobQ → | adenosyl‑cobyric acid synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,872,685 | 0 | G | A | 9.9% | 165.3 / 4.5 | 60 | G310S (GGT→AGT) | cobQ | adenosyl‑cobyric acid synthase |
| Reads supporting (aligned to +/- strand): ref base G (28/25); new base A (3/3); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCACACCGACTTCGACCCGCTGCGCCTGCACCCGCAGGTGGACTTGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAGGCTCAAGCCAGGGGCTCGGTCTGCTCG > NC_002947/1872540‑1872832 | ccACACCGACTTCGACCCGCTGCGCCTGCACCCGCAGGTGGACTTGCAGTTCATTGGCCCCGGCCGGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTg > 2:395533/1‑150 (MQ=255)ccACACCGACTTCGACCCGCTGCGCCTGCACCCGCAGGTGGACTTGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTg < 2:916918/150‑1 (MQ=255)ccACACCGACTTCGACCCGCTGCGCCTGCACCCGCAGGTGGACTTGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGATTg > 2:1149685/1‑150 (MQ=255) cGACTTCGACCCGCTGCGCCTGCACCCGCAGGTGGACTTGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACaa > 1:1043615/1‑150 (MQ=255) gACTTCGACCCGCTGCGCCTGCACCCGCAGGTGGACTTGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAg < 2:845349/150‑1 (MQ=255) cccGCTGCGCCTGCACCCGCAGGTGGACTTGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGa > 1:1094128/1‑150 (MQ=255) ccGCTGCGCCTGCACCCGCAGGTGGACTTGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCCCGAGTGTGCGCGGCGACCTGTCCCAACCGCGCTCGCGCAGTTgtgc > 1:837671/1‑134 (MQ=255) ccGCTGCGCCTGCACCCGCAGGTGGACTTGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGTCCCAACCGCGCTCGCGCAGTTgtgc < 2:837671/137‑4 (MQ=255) ccTGCACCCGCAGGTGGACTTGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCt > 1:1073726/1‑150 (MQ=255) ggTGGACTTGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATggcgg < 1:398680/150‑1 (MQ=255) ttGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACCGCGCTCGCGCAGTTgt > 1:1234170/1‑107 (MQ=255) gCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGAt < 2:1073726/150‑1 (MQ=255) gTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCgg > 1:1138474/1‑150 (MQ=255) ttCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTgg < 1:1289018/102‑1 (MQ=255) ttCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTgg > 2:1289018/1‑102 (MQ=255) ttCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGt > 2:1506630/1‑150 (MQ=255) ttCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCGGATCGGt > 1:277481/1‑150 (MQ=255) cccAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCgg > 2:1482549/1‑150 (MQ=255) tCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACCGCGCTCGCGCAGTTgt < 2:1362619/79‑2 (MQ=38) tCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACCGCGCTCGCGCAGTTgt > 1:1362619/1‑78 (MQ=38) ccGACTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCCTCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTg < 2:762207/150‑1 (MQ=255) cGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTgg < 1:186431/150‑1 (MQ=255) ggCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTggg > 1:439679/1‑150 (MQ=255) ccGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCTGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCggg < 1:805205/150‑1 (MQ=255) ccGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCggg < 2:1138474/150‑1 (MQ=255) tcatcaTCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTAt < 1:915185/115‑1 (MQ=255) tcatcaTCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTAt > 2:915185/1‑115 (MQ=255) atcatcTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAg > 1:634653/1‑102 (MQ=255) atcatcTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAg < 2:634653/102‑1 (MQ=255) atcatcTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCAt < 2:49606/150‑1 (MQ=255) tcatcTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATg > 2:592663/1‑150 (MQ=255) tcTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACaa < 1:1129060/64‑1 (MQ=255) tcTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACaa < 1:1129607/64‑1 (MQ=255) tcTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACaa > 2:1129060/1‑64 (MQ=255) tcTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACaa > 2:1129607/1‑64 (MQ=255) cGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCGACGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGAGGCTGGGGCGGGAAGTGCATGACCGGCTgg > 2:362417/1‑150 (MQ=255) ggCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGATTGGGGCAAGGCCATCGAGCGGCACCTGCGCTATGTCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTgggg < 1:1149685/150‑1 (MQ=255) tCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCt > 2:1298781/1‑100 (MQ=255) tCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCt < 1:1298781/100‑1 (MQ=255) tCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTg > 2:522598/1‑150 (MQ=255) ccAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTgg < 2:1043615/150‑1 (MQ=255) cAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGTGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCACGCTGATCGGCATCTG‑GGAGTTCGGCCAGGTCCTCGCGCGGGAAGCGCAGGACCCGCTGGGGCTGGa > 2:1177843/1‑150 (MQ=255) gagTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTAt > 2:1132938/1‑73 (MQ=255) gagTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTAt < 1:1132938/73‑1 (MQ=255) gagTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAggg > 1:1123316/1‑150 (MQ=255) agTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAgggg < 1:607188/150‑1 (MQ=255) tgtgCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGc > 2:320114/1‑150 (MQ=255) gcggcgACCGGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAg < 1:1177843/150‑1 (MQ=255) cggcgACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAgg < 2:1094128/150‑1 (MQ=255) gcgACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAGGCt < 1:395533/150‑1 (MQ=255) gACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAGGCTCa > 2:756295/1‑150 (MQ=255) aCCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAGGCTCaa < 1:320114/150‑1 (MQ=255) cTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTgg > 2:1079268/1‑124 (MQ=255) cTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTgg < 1:1079268/124‑1 (MQ=255) gCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAGGCTCAAGCCAg < 1:592663/150‑1 (MQ=255) ccaACCGCGCTCGCGCAGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAGGCTCAAGCCAggg < 2:1249499/149‑1 (MQ=255) acaACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAGGCTCAAGCCAggg > 2:238049/1‑150 (MQ=255) gcgAGCGCGGTTTGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAGGCTCAAGCCAGGGGCTCGGTc > 2:1436919/1‑150 (MQ=255) gAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGAGACTGGAGGGGGCCGCAGGCTCAAGCCAGGGGCTCGGTCTg > 1:906032/1‑150 (MQ=255) aGCGCGGTTTGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAGGCTCAAGCCAGGGGCTCGGTCTGc < 1:1436919/150‑1 (MQ=255) gcgGTTGGGACAAGGCCATCGAGCAGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAGGCTCAAGCCAGGGGCTCGGTCTGCTCg < 1:763771/150‑1 (MQ=255) | CCACACCGACTTCGACCCGCTGCGCCTGCACCCGCAGGTGGACTTGCAGTTCATTGGCCCAGGCCAGCCGATTCCGGCTGCCGACCTCATCATCTTGCCGGGCTCCAAGAGTGTGCGCGGCGACCTGGCACAACTGCGCGAGCGCGGTTGGGACAAGGCCATCGAGCGGCACCTGCGCTATGGCGGCAAGCTGATCGGTATCTGCGGTGGCCTG‑CAGATGCTGGGGCGGGAAGTGCATGACCCGCTGGGGCTGGAGGGGGCCGCAGGCTCAAGCCAGGGGCTCGGTCTGCTCG > NC_002947/1872540‑1872832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |