Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,320,847 A→C 7.2% V146G (GTG→GGG)  PP_2041 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,320,8470AC 7.2% 155.6 / 2.0 55V146G (GTG→GGG) PP_2041LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (27/24);  new base C (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

GGGTGGCATTACCAGGCGGCTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGAGGCAGTGGCGGGAATC  >  NC_002947/2320702‑2320996
                                                                                                                                                 |                                                                                                                                                     
gggcggCATTACCAGGCGGCTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGcaccac                                                                                                                                                   <  1:908403/146‑1 (MQ=255)
       aTTACCAGGCGGCTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGaaa                                                                                                                                            <  2:1469900/150‑1 (MQ=255)
         tACCAGGCGGCTCGCTGCGGCTTCAGGCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGAGCCAATATAGGTCTTCGGAAAACACGGGCTCGCCGACCACATGGACAAGTTCTACGGCGTGGTGCCCAACGCCGaagaa                                                                                                                                          <  1:129152/150‑4 (MQ=255)
          aCCAGGCGGCTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATcc                                                                                                                                         >  1:167195/1‑150 (MQ=255)
                  gcTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGcc                                                                                                                                 <  2:1282913/150‑1 (MQ=255)
                        tGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTc                                                                                                                           >  1:828695/1‑150 (MQ=255)
                        tGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTc                                                                                                                           <  2:678588/150‑1 (MQ=255)
                              ttCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACGAGTTCTTCGGCGTTGGGCACCACGCAGAAATCCACCTTGCCCAGATcacacgc                                                                                                                     >  1:1572182/1‑144 (MQ=255)
                                 aGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTg                                                                                                                  >  2:1450296/1‑150 (MQ=255)
                                   ccAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGc                                                                                                                >  2:1501808/1‑150 (MQ=255)
                                             gggCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTcgcg                                                                                                      >  1:1039384/1‑150 (MQ=255)
                                                    tCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAgg                                                                                               >  1:934020/1‑150 (MQ=255)
                                                    tCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAgg                                                                                               <  2:934020/150‑1 (MQ=255)
                                                      gTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGcc                                                                                             >  2:962799/1‑150 (MQ=255)
                                                       tCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCa                                                                                            >  2:1390846/1‑150 (MQ=255)
                                                       tCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGACTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCATCTTCGCGGCTCAGGccc                                                                                            >  2:419506/1‑149 (MQ=255)
                                                              cGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCaaa                                                                                     <  1:1390846/150‑1 (MQ=255)
                                                                  cccAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGt                                                                                 <  2:756399/150‑1 (MQ=255)
                                                                  cccAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGt                                                                                 <  1:1501808/150‑1 (MQ=255)
                                                                   ccAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTg                                                                                >  2:159233/1‑150 (MQ=255)
                                                                    caggcaggCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTTGCCACTTTCGCGGCTCAGGCCATCCCAAACGGTGa                                                                               >  1:806963/1‑150 (MQ=255)
                                                                    caggcaggCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGa                                                                               >  2:26548/1‑150 (MQ=255)
                                                                           gcCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAACCGGTGAGCTTGAt                                                                        >  2:286937/1‑150 (MQ=255)
                                                                                     tACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAg                                                              <  2:1204116/150‑1 (MQ=255)
                                                                                             ttCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTTTTCGGCGTGGTGCCCAACGCCGaagaa                                                                                                                                          <  2:998266/66‑4 (MQ=21)
                                                                                             ttCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTTTTCGGCGTGGTGCCCAACGCCGaagaa                                                                                                                                          >  1:998266/1‑63 (MQ=18)
                                                                                             ttCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGt                                                           >  2:1021236/1‑145 (MQ=255)
                                                                                             ttCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGt                                                           <  1:1021236/145‑1 (MQ=255)
                                                                                              tCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTgcctgg                                                     <  2:167195/150‑1 (MQ=255)
                                                                                               cGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTgcctggg                                                    >  1:39796/1‑150 (MQ=255)
                                                                                               cGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCGGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTgcctggg                                                    <  2:1123548/150‑1 (MQ=255)
                                                                                                  aaaaCACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGcc                                                                                                               >  1:1481198/1‑88 (MQ=255)
                                                                                                  aaaaCACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGcc                                                                                                               <  2:1481198/88‑1 (MQ=255)
                                                                                                         gggCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAat                                          <  1:599206/150‑1 (MQ=255)
                                                                                                           gCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAatga                                        >  2:1285866/1‑150 (MQ=255)
                                                                                                            cTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAatgat                                       <  2:39796/150‑1 (MQ=255)
                                                                                                             tCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAatgatg                                      <  1:962799/150‑1 (MQ=255)
                                                                                                              cGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATgg                                     <  1:966196/150‑1 (MQ=255)
                                                                                                               ggCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACATTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGc                                    >  1:369072/1‑150 (MQ=255)
                                                                                                                 cGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCg                                  <  2:1039384/150‑1 (MQ=255)
                                                                                                                  gACCAAATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCgg                                 >  1:606728/1‑150 (MQ=255)
                                                                                                                            aCAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAAc                                                                                    <  1:376316/89‑1 (MQ=255)
                                                                                                                            aCAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAAc                                                                                    >  2:376316/1‑89 (MQ=255)
                                                                                                                            aCAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGt                       >  1:37524/1‑150 (MQ=255)
                                                                                                                             cAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTg                      <  1:159233/150‑1 (MQ=255)
                                                                                                                              aaGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCgg                                                                  <  1:588037/105‑1 (MQ=255)
                                                                                                                              aaGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCgg                                                                  >  2:588037/1‑105 (MQ=255)
                                                                                                                                gTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATTGCCTGGCACCCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATTCCCGGGAAGCGTGCGTGGGCCTGGGTAATGAGGGCCGGCAACAACAGTGCCg                   >  2:1580924/1‑150 (MQ=255)
                                                                                                                                gTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTaa                                           >  1:33654/1‑126 (MQ=255)
                                                                                                                                gTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTaa                                           <  2:33654/126‑1 (MQ=255)
                                                                                                                                gTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCg                   >  1:325642/1‑150 (MQ=255)
                                                                                                                                 gatttcGGCGTGGTGCCCAACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGa                  >  1:1114531/4‑150 (MQ=255)
                                                                                                                                  tcttcGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGAg                 <  2:812239/150‑1 (MQ=255)
                                                                                                                                   cttcGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGAgg                <  2:606728/150‑1 (MQ=255)
                                                                                                                                    tacGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGAGGc               >  1:714386/3‑150 (MQ=255)
                                                                                                                                           ttGGGCACCACGCCGAAATCCACCTTTCCCAGCTTGATCGCTTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACTTTGCCGAGGCAGTGGCg        <  2:1572182/150‑1 (MQ=255)
                                                                                                                                                 accacGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGAGGCAGTGGCGGGAATc  <  2:714386/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GGGTGGCATTACCAGGCGGCTCGCTGCGGCTTCAGCCAAGGTGATGGGCGACTCGTCAGGGCCGTGCCCAGGCAGGCCGACCCAATACAGGTCTTCGGAAAACACGGGCTCGGCGACCACATGGACAAGTTCTTCGGCGTTGGGCACCACGCCGAAATCCACCTTGCCCAGCTCGATCGCCTGGCCACCTTCGCGGCTCAGGCCATCCCAAACGGTGAGCTTGATCCCCGGGAAGCGTGCCTGGGCCTGGGTAATGATGGCCGGCAACAACAGTGCCGAGGCAGTGGCGGGAATC  >  NC_002947/2320702‑2320996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑