| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 343,611 | G→C | 11.1% | intergenic (‑164/‑76) | PP_5439 ← / → PP_5440 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 343,611 | 0 | G | C | 11.1% | 132.4 / 8.6 | 45 | intergenic (‑164/‑76) | PP_5439/PP_5440 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (20/20); new base C (3/2); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
GTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCGGTTTCGC > NC_002947/343462‑343760 | gTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATg < 2:1374776/150‑1 (MQ=255) gAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGtt < 2:1359245/150‑1 (MQ=255) aGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCAACATGGCTGGCAg < 2:1637868/150‑1 (MQ=255) gcgcGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCAACATGGCTGGCa > 1:1239752/1‑146 (MQ=255) gcgcGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCAACATGGCTGGCa < 2:1239752/146‑1 (MQ=255) gTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCACCCATGTTGGCTGGCAGCCTGTCa > 1:168784/1‑150 (MQ=255) gcgcAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCa > 2:644109/1‑150 (MQ=255) cgcAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCaa > 1:9374/1‑150 (MQ=255) aaTTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTc < 2:9374/150‑1 (MQ=255) ccGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTc > 2:1024835/1‑150 (MQ=255) aaaCCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCgtga < 1:1212481/150‑1 (MQ=255) ccAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTcc > 2:400329/1‑142 (MQ=255) ccAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTcc < 1:400329/142‑1 (MQ=255) ccAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCAGGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCgtgagtga > 1:917811/1‑150 (MQ=255) aGCGGCGCGCCTGGGCGCTGCAGCTCGGCAGGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATgc < 1:820713/150‑1 (MQ=255) gcgcCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGt > 1:827814/1‑150 (MQ=255) gtcTGGGCGCCTCAGCTTGAGATGTCTGTGGGATACATACCAAGATTATTCGGCCCGTGGGCTCTGCGTAGCTTCTCTGCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCCCTGCGCCAATTCGGCGTCCGTGACTGCTGCGCACGttt < 2:748601/148‑1 (MQ=255) gcCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGt > 1:24559/1‑95 (MQ=255) gcCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGt < 2:24559/95‑1 (MQ=255) gggCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTgcgc < 1:474782/150‑1 (MQ=255) gCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAag > 1:1208194/1‑150 (MQ=255) cAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAagg < 2:1003912/150‑1 (MQ=255) gCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAaggag < 2:168784/150‑1 (MQ=255) ttGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACaaa < 1:1156084/144‑1 (MQ=255) ttGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACaaa > 2:1156084/1‑144 (MQ=255) tCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCAc < 2:484743/122‑1 (MQ=255) tCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCAc > 1:484743/1‑122 (MQ=255) cTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAg < 1:323247/150‑1 (MQ=255) tgtgGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGc < 2:1208194/150‑1 (MQ=255) ggATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACtt > 2:1159596/1‑150 (MQ=255) tCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTgc < 2:1634731/113‑1 (MQ=255) tCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTgc > 1:1634731/1‑113 (MQ=255) gACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGc > 1:40654/1‑150 (MQ=255) gACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACAGc > 1:468393/1‑150 (MQ=255) cGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCt < 1:782531/53‑1 (MQ=255) cGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCt > 2:782531/1‑53 (MQ=255) tGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTacac < 2:827814/150‑1 (MQ=255) cTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGa < 1:644109/150‑1 (MQ=255) gTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGc < 2:40654/150‑1 (MQ=255) aGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAg > 1:1328080/1‑150 (MQ=255) tctaCTGCTGCCAGCCATGTGTTCTGGCAGCCTTGCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTa < 2:1328080/150‑1 (MQ=255) tcCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACt > 1:578269/1‑150 (MQ=255) aggctgcCAGCCAACATGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTAt > 2:707413/3‑150 (MQ=255) tgcCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCaa > 1:996116/1‑150 (MQ=255) tgcCAGCCAACATGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCaa > 1:1404341/1‑150 (MQ=255) agccaTGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGCCGTCCGTGCTCGATGCGCACGCTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCgg > 1:1266487/1‑150 (MQ=255) gTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAg < 1:149416/82‑1 (MQ=255) gTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAg > 2:149416/1‑82 (MQ=255) gTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCGGTTTCGc < 2:578269/150‑1 (MQ=255) | GTGAGTCTGAAAGTGCGCGTATTGAAGCGCAATTGCCCGATAAACCCAAGCGGCGCGCCTGGGCGCTGCAGCTTGAGATGTCTGTGGGATACATATCCAGATTATTCGGACCGTGGGCCCTGCGTAGCTTCTCTCCTGCTGCCAGCCATGTTGGCTGGCAGCCTGTCACTGCGCCAATTCGGCGTCCGTGAGTGATGCGCACGTTTGCGCTACAAAGGAGGAGTCATGAAGCACTTTTATCTGGTCACCCTGTACGGCTACACGGACGATGGCAGGGTTTACTATCCAACCGGTTTCGC > NC_002947/343462‑343760 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |