| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,514,648 | C→T | 6.7% | A130T (GCA→ACA) | ftsK ← | DNA translocase FtsK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,514,648 | 0 | C | T | 6.7% | 177.4 / 2.8 | 60 | A130T (GCA→ACA) | ftsK | DNA translocase FtsK |
| Reads supporting (aligned to +/- strand): ref base C (27/29); new base T (2/2); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
GCCGAACAGGAACAGGGCAATGAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGAAAATATAGGCGAAGTAACCCA > NC_002947/4514502‑4514794 | gCCGAACAGGAACAGGGCAATGAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTgccgc > 2:987042/1‑150 (MQ=255) gaacaggaacagGGCAATGAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGcccc < 1:1140520/150‑1 (MQ=255) gaacaggaacagGGCAATGAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGcccc > 2:1259318/1‑150 (MQ=255) aacaggaacaggGCAATGAACATCACCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGccccc < 1:661854/150‑1 (MQ=255) aggaacaggGCAATGAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGAc > 2:1627322/1‑150 (MQ=255) ggaacaggGCAATGAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACa < 1:1326136/150‑1 (MQ=255) aggGCAATGAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCAcc > 2:316767/1‑150 (MQ=255) gggCAATGAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCa > 1:955760/1‑150 (MQ=255) ggCAATGAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAg > 1:708057/1‑150 (MQ=255) ggCAATGAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAg < 1:22897/150‑1 (MQ=255) gAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAacac > 2:681169/1‑150 (MQ=255) tCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGc < 1:1259318/150‑1 (MQ=255) gCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCaa < 2:708057/150‑1 (MQ=255) gTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATc < 2:24413/150‑1 (MQ=255) tgcCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAgcc < 2:1107168/150‑1 (MQ=255) tgcCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAgcc > 1:638165/1‑150 (MQ=255) cccTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAgccgc > 1:1016360/1‑150 (MQ=255) cTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGTCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCa > 2:330185/1‑150 (MQ=255) tGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAg < 1:316767/150‑1 (MQ=255) cACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGTCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGga < 1:330185/150‑1 (MQ=255) cACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAacac < 1:414393/129‑1 (MQ=255) cACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAacac > 2:414393/1‑129 (MQ=255) cGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGgaga < 1:987042/150‑1 (MQ=255) agcagGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAAcagc > 1:1290183/1‑150 (MQ=255) agcagGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTAAAAGAAGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAAcagc > 1:691550/1‑150 (MQ=255) gcagGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAAcagcc > 2:414496/1‑150 (MQ=255) ggTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAAcagccagc < 2:955760/150‑1 (MQ=255) gcgcCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAgg > 2:33580/1‑116 (MQ=255) gcgcCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAgg < 1:33580/116‑1 (MQ=255) gcgcCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGgag < 1:600648/134‑1 (MQ=255) gcgcCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGgag > 2:600648/1‑134 (MQ=255) cgcCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCa < 1:213102/150‑1 (MQ=255) cgcCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCa < 1:414496/150‑1 (MQ=255) gCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACgg > 1:270818/1‑150 (MQ=255) ggCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCtggt < 2:1290183/150‑1 (MQ=255) tttCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGTCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGc > 2:440105/1‑150 (MQ=255) tttCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGTCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGc < 1:440105/150‑1 (MQ=255) cccAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAg > 1:1514678/1‑108 (MQ=255) cccAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAg < 2:1514678/108‑1 (MQ=255) cACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAAt < 1:1536241/148‑1 (MQ=255) cACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAAt > 2:1536241/1‑148 (MQ=255) cACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCt < 1:681169/150‑1 (MQ=255) gaACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGtttt > 2:707303/1‑150 (MQ=255) gaACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGtttt < 1:1168863/150‑1 (MQ=255) ggCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGAcc < 1:1623378/150‑1 (MQ=255) gCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCa < 1:1143427/75‑1 (MQ=255) gCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCa > 2:1143427/1‑75 (MQ=255) aaGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGAAAata > 1:639339/1‑150 (MQ=255) aaGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGAAAata < 1:1627322/150‑1 (MQ=255) tGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGAAAATATAg > 2:1463118/1‑150 (MQ=255) gATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGga < 2:1109766/60‑1 (MQ=255) gATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGga > 1:1109766/1‑60 (MQ=255) gATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGAAAATATAGGc > 1:787258/1‑150 (MQ=255) atGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCAAGCAGCAGCGGGAAAATATAGGCGaa < 1:1345502/150‑1 (MQ=255) cgcCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGAAAATATAGGCGaa > 2:101406/1‑147 (MQ=255) cgcCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGAAAATATAGGCGaa < 1:101406/147‑1 (MQ=255) gcCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGaaa > 2:1403846/1‑135 (MQ=255) gcCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGaaa < 1:1403846/135‑1 (MQ=255) gcCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGAAAATATAGGCGAAGTaa > 1:1308787/1‑150 (MQ=255) gTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGAAAATATAGGCGAAGTAACCCa < 2:691550/150‑1 (MQ=255) gTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGAAAATATAGGCGAAGTAACCCa > 1:441465/1‑150 (MQ=255) | GCCGAACAGGAACAGGGCAATGAACATCAGCGTGCTGCCCTGCACGTTCAGCAGGTTGCGCGCCAGGTCGCCCAGGCTTTCGCCCAGTGCTCCACCGGCGGAGAACGGCAGGCTGGCGGGCGGGTGGAAGTGGATATGCGCCAGTGCCGCCCCCGACAGCACCAGGAACACCAGGCCAATCAGCCGCCAGGAGAACAGCCAGCCGCTCCAGTCCCACGGCTGGTGGCGTTCGCGGAAAATCTGCCAGGTTTTGACCGCCAGCAGCAGCGGGAAAATATAGGCGAAGTAACCCA > NC_002947/4514502‑4514794 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |