| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,758,192 | A→G | 10.1% | intergenic (+7/+14) | ompQ → / ← pvdP | outer membrane pyoverdine efflux protein/pyoverdine biosynthesis‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,758,192 | 0 | A | G | 10.1% | 160.2 / 7.1 | 60 | intergenic (+7/+14) | ompQ/pvdP | outer membrane pyoverdine efflux protein/pyoverdine biosynthesis‑like protein |
| Reads supporting (aligned to +/- strand): ref base A (26/27); new base G (3/3); total (30/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
TGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGA > NC_002947/4758046‑4758332 | tGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGc > 2:1201891/1‑150 (MQ=255) ccGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCTGACGCCCTAGCGCGTCGGGCTAt < 1:143111/150‑1 (MQ=255) tGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTg < 2:1353129/150‑1 (MQ=255) gCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTgg < 1:1201891/150‑1 (MQ=255) acCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTg < 2:1294509/142‑1 (MQ=255) acCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTg > 1:1294509/1‑142 (MQ=255) acCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCAc < 1:1581060/150‑1 (MQ=255) cccAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCAcc > 2:1638092/1‑149 (MQ=255) ccAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGa > 1:1465220/1‑150 (MQ=255) ccAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCtt > 2:763480/1‑150 (MQ=255) ggCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAg < 1:763480/150‑1 (MQ=255) ggCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAg > 1:509828/1‑150 (MQ=255) ggACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCAGATAGCCTGACGCCCTAGCGCGTCGGGCt < 1:91872/111‑1 (MQ=38) ggACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCAGATAGCCTGACGCCCTAGCGCGTCGGGCt > 2:91872/1‑111 (MQ=38) gACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGa < 2:1465220/150‑1 (MQ=255) ccAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCTCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGc < 1:1020660/150‑1 (MQ=255) ccAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGc < 2:1445353/150‑1 (MQ=255) gCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGgcggcg < 1:1113452/150‑1 (MQ=255) cAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGgcggcgc > 2:556055/1‑150 (MQ=255) cAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGgcggcgc > 1:1144437/1‑150 (MQ=255) aGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc > 1:1231157/1‑109 (MQ=255) aGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc < 2:1231157/109‑1 (MQ=255) ccAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAg < 2:315602/81‑1 (MQ=255) ccAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAg > 1:315602/1‑81 (MQ=255) ccAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACttt > 1:1294324/1‑150 (MQ=255) cAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTg < 1:165687/150‑1 (MQ=255) aaCTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGc < 1:556055/150‑1 (MQ=255) gCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTAc < 1:1040080/150‑1 (MQ=255) gCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGc < 1:879330/96‑1 (MQ=255) gCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGc > 2:879330/1‑96 (MQ=255) gCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGt > 2:1368023/1‑150 (MQ=255) ggCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCaaa < 2:671539/150‑1 (MQ=255) gCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGc > 1:407023/1‑84 (MQ=255) gCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGc < 2:407023/84‑1 (MQ=255) cTTTACAAAGCCTTGGGCGGTGGCTGGCAAGTGGATAGCCCGACGCGCTAGGGCGTCCGGCTATCTGGTCTTAAAACAGGAAGCCGCTAAGCGAGATGGCGGGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAgcgc > 1:1192406/1‑150 (MQ=255) tACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcc > 2:294697/1‑150 (MQ=255) cAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGTGGCGGCGCGGCACtt > 2:1603425/1‑122 (MQ=255) cAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGTGGCGGCGCGGCACtt < 1:1603425/122‑1 (MQ=255) cTTGGGCGGTGGCTGGCAAGAGGATAGCCCGACGCGCTAGGGCGGCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGCGGCGACTGGGCACTCTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCt > 2:358005/1‑150 (MQ=255) tggcAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGgcgc > 1:589498/1‑150 (MQ=255) gcAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCaa > 1:1326856/1‑150 (MQ=255) cAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAg < 1:358005/150‑1 (MQ=255) aaGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTgg < 2:1268033/71‑1 (MQ=255) aaGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTgg > 1:1268033/1‑71 (MQ=255) aaGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAgg > 2:908184/1‑150 (MQ=255) cctgACGCCCTAGCGCGTCGGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCAt > 1:721427/4‑150 (MQ=255) ctgACGCCCTAGCGCGTCGGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATg < 2:721427/148‑1 (MQ=255) ccGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAgggg > 2:246610/1‑75 (MQ=255) ccGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAgggg < 1:246610/75‑1 (MQ=255) ccGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATg < 2:1144437/150‑1 (MQ=255) ccGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATg < 2:509828/150‑1 (MQ=255) cGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATgg < 1:911362/150‑1 (MQ=255) gACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTACGCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGt > 1:1320490/1‑150 (MQ=255) ccTAGCGCGTCGGGCTATCCGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCg > 1:185272/2‑150 (MQ=255) tAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATgg > 2:865893/1‑142 (MQ=255) tAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATgg < 1:865893/142‑1 (MQ=255) tAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGa > 2:1481073/1‑150 (MQ=255) tAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGa < 2:1326856/150‑1 (MQ=255) tCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCtcgtc > 1:968670/1‑103 (MQ=255) tCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCtcgtc < 2:968670/103‑1 (MQ=255) | TGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAGGCGCAAGGCCAGCTTCATGGTTTCCGGA > NC_002947/4758046‑4758332 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |