| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,384,575 | G→C | 8.6% | G331R (GGC→CGC) ‡ | lldP → | L‑lactate permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,384,575 | 0 | G | C | 8.6% | 177.6 / 8.4 | 59 | G331R (GGC→CGC) ‡ | lldP | L‑lactate permease |
| Reads supporting (aligned to +/- strand): ref base G (28/24); new base C (2/3); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01 | |||||||||||
GGCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACCGCGATCTTC > NC_002947/5384431‑5384724 | ggCGCAGCTGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGCTCTTCAAGGTGGGCTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGggcggc < 1:1264028/150‑1 (MQ=255) gCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGCCCGGCGCGaaa < 1:1578673/150‑3 (MQ=255) ttGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACa > 2:473924/1‑150 (MQ=255) cAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTc < 1:663172/150‑1 (MQ=255) cAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtgt > 2:1402050/1‑150 (MQ=255) aGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTc < 1:1543496/150‑1 (MQ=255) ccagccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGc < 1:1207225/150‑1 (MQ=255) ccTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATc < 2:1175232/150‑1 (MQ=255) tAGCCCGTACAGCGTTGGCGAGGTCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATccc < 1:1059645/150‑1 (MQ=255) cTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCa > 2:718903/1‑150 (MQ=255) tttGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAg > 2:1240038/1‑150 (MQ=255) cGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGt < 1:473924/150‑1 (MQ=255) agaTCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGa > 2:96558/1‑150 (MQ=255) aTCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATc < 1:9999/150‑1 (MQ=255) aTCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAAATTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATc < 2:918977/150‑1 (MQ=255) ttCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtg > 1:416209/1‑108 (MQ=255) ttCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtg < 2:416209/108‑1 (MQ=255) ccTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCAc < 1:1240038/150‑1 (MQ=255) gTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtg < 1:68857/97‑1 (MQ=255) gTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCgtg > 2:68857/1‑97 (MQ=255) tCGCCGTTCTTGCTCCGCTCCGTGGTGGTCACCATCGCGACCCTGAAGCCATTCAAGGCGGCTTTCGCGCCGTGCTGCGCGATGTAAAACTTCGTGTTCAACTTCGCGATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACTGATc < 2:274199/150‑1 (MQ=255) gTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGGCAGTGATCAAGACCGCACCGATCGTTGc > 1:1228/1‑150 (MQ=255) gTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCAcc > 1:406261/1‑141 (MQ=255) gTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCAcc < 2:406261/141‑1 (MQ=255) gTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGc < 2:1370417/150‑1 (MQ=255) ccTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCcgc > 2:281817/1‑150 (MQ=255) tGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCgcgc > 2:1309131/1‑150 (MQ=255) tCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGTGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGa < 2:1040782/150‑1 (MQ=255) ccTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTc < 1:1605676/106‑1 (MQ=255) ccTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTc > 2:1605676/1‑106 (MQ=255) cTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATc > 1:865282/1‑135 (MQ=255) cTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATc < 2:865282/135‑1 (MQ=255) cTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGAcc > 1:1512112/1‑150 (MQ=255) tGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCcgg > 1:583009/1‑150 (MQ=255) gTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCcggcg > 2:892397/1‑150 (MQ=255) tCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCcggcgg < 1:1309131/150‑1 (MQ=255) cACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCACGCCGGCGgt > 1:883906/1‑150 (MQ=255) ccATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGgtgt < 2:583009/150‑1 (MQ=255) tGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGtt < 1:240370/117‑1 (MQ=255) tGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGtt > 2:240370/1‑117 (MQ=255) gACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCt > 2:1091635/1‑150 (MQ=255) cccTGAAGCCGGTCAAGGCGGCTTTCGCGCCGGGCCGCGCGATGTACAACTTCCTGTTCAACTTCGCGATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATAGTTGCCCCGCCGACCGCCATTCCGGCGGAGTTCACGCTCg > 2:171190/1‑150 (MQ=255) ccTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGa > 2:341813/1‑150 (MQ=255) cTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGAc > 1:1189321/1‑150 (MQ=255) tGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGAcc > 2:1257611/1‑150 (MQ=255) gCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATACCGCACCTCGGCCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGAt > 1:473381/1‑150 (MQ=255) ccGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGAtc > 1:585481/1‑150 (MQ=255) ttCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTcc < 2:1438707/150‑1 (MQ=255) ttCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTAGACCCGATCTcc < 2:1512112/150‑1 (MQ=255) ggcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGAc < 1:482712/84‑1 (MQ=255) ggcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGAc > 2:482712/1‑84 (MQ=255) ggcggcTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCAc > 2:217321/1‑150 (MQ=255) gtacatCGCGCCGCCCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTcc < 2:1563774/137‑1 (MQ=255) gtacatCGCGCCGCCCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTcc > 1:1563774/6‑142 (MQ=255) tttCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGa > 1:1473643/1‑86 (MQ=255) tttCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGa < 2:1473643/86‑1 (MQ=255) atCGCGCCGCCCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTcc > 1:953172/2‑138 (MQ=255) atCGCGCCGCCCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTcc < 2:953172/137‑1 (MQ=255) tCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCAGCAcgc < 2:585481/150‑3 (MQ=255) cgcgCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACcgc < 2:1485030/150‑1 (MQ=255) ggcggcGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACCGCGATCTTc < 1:718903/150‑1 (MQ=255) | GGCGCAGCCGTTGTGCAGCCAAGCGGCAGCCAGCCTAGCCCGTACAGCTTTGGCGAGATCTTCAAGGCCTGGTCGCCGTTCCTGATCCTCACCGTGCTGGTCACCATCTGGACCCTGAAGCCGTTCAAGGCGGCTTTCGCGCCGGGCGGCGCGATGTACAACTTCGTGTTCAACTTCGCCATCCCGCACCTCGACCAGCTGGTGATCAAGACCGCACCGATCGTTGCCGCGCCGACCGCCATGCCGGCGGTGTTCAAGCTCGACCCGATCTCCGCCACCGGCACCGCGATCTTC > NC_002947/5384431‑5384724 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |