Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,647,612	G→A	D384D (GAC→GAT)	oprB‑II ←	carbohydrate‑selective porin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,647,612	0	G	A	100.0%	85.0 / NA	28	D384D (GAC→GAT)	oprB‑II	carbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

GGCGCCATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGC  >  NC_002947/1647465‑1647746                                                                                                                                                    |                                                                                                                                       ggCGCCATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATc                                                                                                                                      >  1:1105838/1‑150 (MQ=255)          gACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCGTGCACTGGCATGAAGCGCGGGTTGTCGTAATCATAGAggac                                                                                                                             >  2:187784/1‑148 (MQ=255)                      cccgcccgGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCAGAATCATATAGGCCGGCGGCCTGGtt                                                                                                                 >  2:1123182/1‑150 (MQ=255)                                tGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAggcgg                                                                                                       <  1:1012732/150‑1 (MQ=255)                                             gCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGAt                                                                                          >  1:189307/1‑150 (MQ=255)                                                 gTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGtt                                                                                                                 <  2:656670/123‑1 (MQ=255)                                                 gTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGtt                                                                                                                 >  1:656670/1‑123 (MQ=255)                                                   tGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAg                                                                                    <  1:1123182/150‑1 (MQ=255)                                                    ggggCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAgg                                                                                   <  1:187784/150‑1 (MQ=255)                                                      ggCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGt                                                                                 <  2:596019/150‑1 (MQ=255)                                                          tACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGAc                                                                             <  1:713255/150‑1 (MQ=255)                                                                           ccAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGc                                                                                             <  1:1134701/117‑1 (MQ=255)                                                                           ccAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGc                                                                                             >  2:1134701/1‑117 (MQ=255)                                                                            cAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGc                                                           >  1:126100/1‑150 (MQ=255)                                                                                tGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGaaa                                                       >  1:589366/1‑150 (MQ=255)                                                                                  aaTGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAAcc                                                     <  2:189307/150‑1 (MQ=255)                                                                                     gCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGAt                                                  <  1:30804/150‑1 (MQ=255)                                                                                       cGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATgt                                                >  1:469986/1‑150 (MQ=255)                                                                                          aaTACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCACGACGCACTGCTCGTGGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGTCCAGTGCGAAACCGATgtcgt                                             >  2:160949/1‑150 (MQ=255)                                                                                           aTACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATgtcgtc                                            <  2:1105838/150‑1 (MQ=255)                                                                                                            aCTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGa                           >  2:227143/1‑150 (MQ=255)                                                                                                                    tCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCt                   >  2:917685/1‑150 (MQ=255)                                                                                                                     ccTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCtt                  <  2:843036/150‑1 (MQ=255)                                                                                                                      cTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCttt                 <  1:227143/150‑1 (MQ=255)                                                                                                                       tGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTg                <  2:469986/150‑1 (MQ=255)                                                                                                                           cTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATa            <  1:346677/150‑1 (MQ=255)                                                                                                                                     gCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTcctgc  >  2:663525/1‑150 (MQ=255)                                                                                                                                                 gTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCgg                                                                   >  1:680761/1‑73 (MQ=255)                                                                                                                                                    |                                                                                                                                       GGCGCCATCGACCTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGC  >  NC_002947/1647465‑1647746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑