Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,819,831 Δ1 bp 5.3% intergenic (+26/‑449) rpoS → / → PP_1624 RNA polymerase sigma S factor/group II intron‑encoding maturase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,819,8310G. 5.3% 420.6 / 17.2 131intergenic (+26/‑449)rpoS/PP_1624RNA polymerase sigma S factor/group II intron‑encoding maturase
Reads supporting (aligned to +/- strand):  ref base G (61/63);  new base . (3/4);  total (64/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

Only 100 of 133 total aligned reads displayed.

GGATGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGT  >  NC_002947/1819682‑1819977
                                                                                                                                                      |                                                                                                                                                     
ggaTGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCg                                                                                                                                                       <  2:1965327/150‑1 (MQ=255)
 gaTGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCgg                                                                                                                                                      <  2:1319063/150‑1 (MQ=255)
   tGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGATATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTg                                                                                                                                                    >  1:285985/1‑150 (MQ=255)
    gTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGa                                                                                                                                                   >  2:814365/1‑150 (MQ=255)
        ggCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGAtc‑t‑c                                                                                                                                             <  2:1241039/150‑1 (MQ=255)
        ggCTGGAGATCGGCCTTACCCGTGAACGGGAGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGAtc‑t‑c                                                                                                                                             <  2:1608177/150‑1 (MQ=255)
          cTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑Cgg                                                                                                                                           <  1:717683/150‑1 (MQ=255)
            ggAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑Cgggg                                                                                                                                         >  2:83020/1‑150 (MQ=255)
                 tCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCC‑GAGATCAC‑CGGGGCG‑ttt                                                                                                                                   <  2:1891662/150‑1 (MQ=255)
                    gCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCC‑GAGATCAC‑CGGGGCG‑tttttt                                                                                                                                <  2:2307537/150‑2 (MQ=255)
                     ccTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑ttttt                                                                                                                                 <  2:2297334/148‑1 (MQ=255)
                     ccTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑ttttt                                                                                                                                 >  1:2297334/1‑148 (MQ=255)
                     ccTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTtgt                                                                                                                               <  1:83020/150‑1 (MQ=255)
                        tatccGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAATAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTtgtgtg                                                                                                                            <  1:2220601/147‑1 (MQ=255)
                         aCCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTgc                                                                                                                           <  2:1628107/150‑1 (MQ=255)
                         aCCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTgc                                                                                                                           >  1:996963/1‑150 (MQ=255)
                             gTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCa                                                                                                                       <  1:137570/150‑1 (MQ=255)
                              tGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAg                                                                                                                      <  2:1318659/150‑1 (MQ=255)
                                   gggTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATg                                                                                                                 <  1:2004171/150‑1 (MQ=255)
                                    ggTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCATGCCTgg                                                                                                                <  1:2319563/150‑1 (MQ=255)
                                     gTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGgc                                                                                                               >  1:682750/1‑150 (MQ=255)
                                           cAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTg                                                                                                         >  2:1356365/1‑150 (MQ=255)
                                              aTCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTgcgc                                                                                                      <  1:1215793/150‑1 (MQ=255)
                                               tCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGcgcg                                                                                                     >  2:2247008/1‑150 (MQ=255)
                                                  aGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTAt                                                                                                  >  1:1431180/1‑150 (MQ=255)
                                                  aGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTAt                                                                                                  >  1:723417/1‑150 (MQ=255)
                                                  aGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTAt                                                                                                  <  2:1924510/150‑1 (MQ=255)
                                                    gTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATgc                                                                                                <  1:2247008/150‑1 (MQ=255)
                                                       gAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCa                                                                                             >  2:1366942/1‑150 (MQ=255)
                                                         aGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAAc                                                                                           >  1:1485920/1‑150 (MQ=255)
                                                         aGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCCACCCGCTGTTCCATTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAAc                                                                                           <  1:1687801/150‑1 (MQ=38)
                                                          gggCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑ttttt                                                                                                                                 <  2:1445604/111‑1 (MQ=255)
                                                          gggCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑ttttt                                                                                                                                 >  1:1445604/1‑111 (MQ=255)
                                                           ggCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAcccc                                                                                         <  2:682750/150‑1 (MQ=255)
                                                            gCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCg                                                                                        >  1:2078154/1‑150 (MQ=255)
                                                            gCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGACCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCg                                                                                        >  1:1741238/1‑150 (MQ=255)
                                                            gCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCGAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TCTTTGTTCGCCGTATGCTTGGCGCGTTGCGCGTATGCGCAACCCg                                                                                        >  2:187344/1‑150 (MQ=255)
                                                                aaGCGCTTGCGCGAGCTCCTTGCAATGACGCGTTCTG‑CCAGCTATTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTg                                                                                    <  2:255498/150‑1 (MQ=25)
                                                                aaGCGCTTGCGCGAGATCCTTGGCCAGC‑CTGGATGCTCGGGTCAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTg                                                                                    <  2:1776364/150‑1 (MQ=25)
                                                                aaGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTg                                                                                    >  2:851413/1‑150 (MQ=255)
                                                                     cTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgt                                                                               <  1:1367961/150‑1 (MQ=255)
                                                                           cgAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGc                                                                         <  1:893108/150‑1 (MQ=255)
                                                                             agaTCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTg                                                                                    <  1:577197/137‑1 (MQ=255)
                                                                               aTCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGACCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAg                                                                     <  2:1741238/150‑1 (MQ=255)
                                                                                tCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGt                                                                    <  2:1872822/150‑1 (MQ=255)
                                                                                 ccTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTg                                                                   >  2:1587354/1‑150 (MQ=255)
                                                                                  cTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGTTTGTGGTGGCCAAGTgg                                                                  <  2:2078154/150‑1 (MQ=255)
                                                                                   ttGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg                                                                              <  1:1810272/137‑1 (MQ=255)
                                                                                   ttGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTggg                                                                 >  2:632704/1‑150 (MQ=255)
                                                                                   ttGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGGGAGG‑T‑CGGGGAG‑ATATCGCGAGCTCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGGGTGGGGTAAGTggg                                                                 >  1:165875/1‑150 (MQ=25)
                                                                                    tGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                                <  1:720556/150‑1 (MQ=255)
                                                                                     gAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTg                                                               >  1:40684/1‑150 (MQ=255)
                                                                                      aaaaGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGTAACCCGCTCCGCCGCGGTGGCTAAGTGGGTgc                                                              >  2:382539/1‑149 (MQ=37)
                                                                                       aaaGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                                <  2:285559/147‑1 (MQ=255)
                                                                                       aaaGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                                >  1:285559/1‑147 (MQ=255)
                                                                                       aaaGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGAc                                                             >  2:1498085/1‑150 (MQ=255)
                                                                                        aaGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACt                                                            >  1:2137271/1‑150 (MQ=255)
                                                                                           a‑aTGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCACGGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTgg                                                         >  2:317922/1‑150 (MQ=255)
                                                                                             aTGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGa                                                        <  1:814365/150‑1 (MQ=255)
                                                                                                 tCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCaa                                                                      >  1:2293293/1‑132 (MQ=255)
                                                                                                    gTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                                 <  1:1498085/150‑1 (MQ=255)
                                                                                                     tCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCATGCCTGgcgcac                                                                                                           >  1:403213/1‑89 (MQ=255)
                                                                                                     tCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCATGCCTGgcgcac                                                                                                           <  2:403213/91‑3 (MQ=255)
                                                                                                        aGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                                 <  1:1440093/146‑1 (MQ=255)
                                                                                                          cGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCctc                                           <  1:847733/150‑1 (MQ=255)
                                                                                                                aTTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑Cggg                                                                                                                                          >  1:1329244/1‑50 (MQ=255)
                                                                                                                aTTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTgtggtg                                                                           <  1:2131857/112‑1 (MQ=255)
                                                                                                                 ttGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTacacac                                    >  1:608499/1‑150 (MQ=255)
                                                                                                                      ccAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTAGGTGGCTTGGAGATGAGAGTCCTCTACACACCCGGc                               >  2:21202/1‑150 (MQ=255)
                                                                                                                       cAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCa                              >  2:1393765/1‑150 (MQ=255)
                                                                                                                            gctagcGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                                 <  2:921432/126‑1 (MQ=255)
                                                                                                                            gctagcGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                                 >  1:921432/1‑126 (MQ=255)
                                                                                                                            gctagcGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAgggg                         >  2:48310/1‑150 (MQ=255)
                                                                                                                                gcgcTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAgt                     <  1:1120536/150‑1 (MQ=255)
                                                                                                                                  ggtGTAACAAAAGACGCCCGGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAgtgt                   <  2:723417/148‑1 (MQ=255)
                                                                                                                                    tGTAACAAAAAACGCCCCGGTGATC‑T‑CGTGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCATACGCGCAAcgc                                                                                         <  2:1736727/78‑3 (MQ=21)
                                                                                                                                    tGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTaca                                       <  1:1990026/128‑1 (MQ=255)
                                                                                                                                    tGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTaca                                       >  2:1990026/1‑128 (MQ=255)
                                                                                                                                    tGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCATACGCGCAAcgc                                                                                         >  1:1736727/1‑76 (MQ=25)
                                                                                                                                      tAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAgg                           >  2:891588/1‑138 (MQ=255)
                                                                                                                                      tAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAACTCCTCTACACACCCGGCAAgg                           <  1:891588/138‑1 (MQ=255)
                                                                                                                                       acacaaaaaCGCCCC‑GAGATC‑ACCGGGGCGTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGt                                                     >  2:244322/5‑112 (MQ=11)
                                                                                                                                       aaCAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATTCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGcc              >  2:2312106/1‑150 (MQ=255)
                                                                                                                                        aCAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTc                                              >  2:463224/1‑117 (MQ=255)
                                                                                                                                          aaaaacCGCCCCGGTGTTT‑T‑CCGTGCG‑TTTTTGTGTCCCCCATGCATGGCGCCTTGCGCGTATGCTCAACCCGCTTGGTTGTGGTGGCCAATTGGGTCACTTGGGGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGa           <  2:1035747/150‑1 (MQ=11)
                                                                                                                                          aaaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCctc                                           >  2:2206470/1‑118 (MQ=255)
                                                                                                                                          aaaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCctc                                           <  1:2206470/118‑1 (MQ=255)
                                                                                                                                          aaaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGa           <  1:382539/150‑1 (MQ=255)
                                                                                                                                          aaaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGCCAAGGGGAAGTGTTAGCCGGa           >  2:42347/1‑150 (MQ=255)
                                                                                                                                           aaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAg          <  2:608499/150‑1 (MQ=255)
                                                                                                                                           aaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAg          <  1:1356365/150‑1 (MQ=255)
                                                                                                                                           aaaaaCGCCCC‑GAGATCAC‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAg          >  1:1379764/1‑150 (MQ=11)
                                                                                                                                           aaaaaCGCCCC‑GAGATC‑ACCGGGGCGTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAgt                     <  1:1518672/140‑1 (MQ=11)
                                                                                                                                           aaaaaCGCCCC‑GAGATC‑ACCGGGGCGTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAgt                     >  2:1518672/1‑140 (MQ=11)
                                                                                                                                              aaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCa       >  1:244490/1‑150 (MQ=255)
                                                                                                                                               aCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCaa      >  1:948238/1‑150 (MQ=255)
                                                                                                                                                cGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAg     <  1:1587354/150‑1 (MQ=255)
                                                                                                                                                   cccGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGgt  >  2:200501/1‑150 (MQ=255)
                                                                                                                                                     cGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTg                                                    >  2:2136634/1‑98 (MQ=255)
                                                                                                                                                     cGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTg                                                    <  1:2136634/98‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GGATGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGT  >  NC_002947/1819682‑1819977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑