BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,336,791	Δ1 bp	6.9%	coding (193/1443 nt)	PP_4696 →	nitrogen regulation protein NR(I)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,336,791	0	A	.	6.9%	311.4 / 17.2	101	coding (193/1443 nt)	PP_4696	nitrogen regulation protein NR(I)
Reads supporting (aligned to +/- strand): ref base A (47/46); new base . (3/4); total (50/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

Only 100 of 103 total aligned reads displayed.

GCCCTGCGTCGCCTGCTTGAGCGGAACCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGATGCTGCAGGCAGTAGCGCGTATTCTGCGT  >  NC_002947/5336644‑5336940

gCCCTGCGTCCCCTGCTTGAGCGGAACCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAg                                                                                                                                                      <  2:1110396/150‑1 (MQ=255)

  ccTGCGTCGCCTGCTTGAGCGGAACCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCt                                                                                                                                                    >  1:94877/1‑150 (MQ=255)

          gCCTGCTTGAGCGGAACCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAgg                                                                                                                                           <  1:2147102/150‑1 (MQ=255)

           ccTGCTTGAGCGGAACCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAggg                                                                                                                                          <  2:1103470/150‑1 (MQ=255)

           ccTGCTTGAGCGGAACCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAggg                                                                                                                                          >  2:1992379/1‑150 (MQ=255)

           ccTGCTTGAGCGGAACCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAggg                                                                                                                                          <  2:2013036/150‑1 (MQ=255)

            cTGCTTGAGCGGAACCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAggg                                                                                                                                          >  1:728666/1‑149 (MQ=255)

            cTGCTTGAGCGGAACCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAggg                                                                                                                                          <  2:728666/149‑1 (MQ=255)

                       gAACCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCt                                                                                                                              >  1:796068/1‑150 (MQ=255)

                         aCCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGa                                                                                                                            <  1:433410/150‑1 (MQ=255)

                           cAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATc                                                                                                                          >  2:944205/1‑150 (MQ=255)

                             gaaCCAGGTTAGGGAGGCCGCCCCGGTGCAGGAAGCCGAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCAt                                                                                                                        <  2:504630/148‑1 (MQ=255)

                                         cGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGGCCAGTTACGc                                                                                                            <  2:796068/150‑1 (MQ=255)

                                         cGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGc                                                                                                            <  2:1916342/150‑1 (MQ=255)

                                                ggCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTg                                                                                                     >  1:1659409/1‑150 (MQ=255)

                                                      gTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCa                                                                                               >  1:2002370/1‑150 (MQ=255)

                                                        gCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGc                                                                                             <  1:1866020/150‑1 (MQ=255)

                                                            gAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTg                                                                                                                                >  1:2054651/1‑111 (MQ=255)

                                                            gAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTg                                                                                                                                <  2:2054651/111‑1 (MQ=255)

                                                            gAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTg                                                                                         <  1:1992379/150‑1 (MQ=255)

                                                            gAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCGGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTg                                                                                         >  1:1589200/1‑150 (MQ=255)

                                                                cccAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCGCCGAGCTTGATC‑AGCTCggtgcc                                                                                                                                             <  2:237356/95‑5 (MQ=255)

                                                                cccAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCGCCGAGCTTGATC‑AGCTCggtgcc                                                                                                                                             >  1:237356/1‑91 (MQ=255)

                                                                 ccAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTCCGCCAGCCTGCGCTCAGCGGTGGGCTc                                                                                    >  1:1543279/1‑150 (MQ=255)

                                                                   aGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGa                                                                                  <  2:94877/150‑1 (MQ=255)

                                                                   aGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGa                                                                                  >  1:1805253/1‑150 (MQ=255)

                                                                          cTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAAt                                                                           <  2:1805253/150‑1 (MQ=255)

                                                                          cTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAAt                                                                           <  1:944205/150‑1 (MQ=255)

                                                                          cTTCAGCATTGCCACTTTCGACCGCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGTCACCGAGC‑TGATCGAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAAt                                                                           <  2:666295/150‑1 (MQ=255)

                                                                           ttCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATAAAGCTCGGC‑CAGGGCACACCGGTGCTGATGATGAGCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATg                                                                          >  1:523673/1‑150 (MQ=255)

                                                                                 aTTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGcgcg                                                                    >  1:1076760/1‑150 (MQ=255)

                                                                                  ttGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAgg                                                                                                                                           <  2:784988/78‑1 (MQ=255)

                                                                                  ttGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAgg                                                                                                                                           >  1:784988/1‑78 (MQ=255)

                                                                                    gCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTg                                                                 <  2:1052007/150‑1 (MQ=255)

                                                                                     ccACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCa                                                                                                                   <  1:955166/99‑1 (MQ=255)

                                                                                     ccACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGACTGTAATCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTgg                                                                >  1:1778580/1‑150 (MQ=255)

                                                                                      cACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAg                                                                                                                                            <  2:128415/73‑1 (MQ=255)

                                                                                      cACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAg                                                                                                                                            >  1:128415/1‑73 (MQ=255)

                                                                                      cACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGt                                                                  <  1:355344/147‑1 (MQ=255)

                                                                                      cACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGt                                                                  >  2:355344/1‑147 (MQ=255)

                                                                                      cACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGa                                                               >  2:43516/1‑150 (MQ=255)

                                                                                      cACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGa                                                               >  2:31598/1‑150 (MQ=255)

                                                                                       aCTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCa                                                                                                          >  2:594886/1‑106 (MQ=255)

                                                                                       aCTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCa                                                                                                          <  1:594886/106‑1 (MQ=255)

                                                                                       aCTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGCGGAc                                                              <  2:1358295/150‑1 (MQ=255)

                                                                                        cTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACt                                                             <  1:31598/150‑1 (MQ=255)

                                                                                         tttCGACCTAATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTa                                                            >  1:1282433/1‑150 (MQ=255)

                                                                                              aCCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCg                                                       >  1:1475229/1‑150 (MQ=255)

                                                                                                cTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGcc                                                     >  1:2093890/1‑150 (MQ=255)

                                                                                                 tCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGa                                                                                                                      >  1:1780331/1‑84 (MQ=255)

                                                                                                 tCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGa                                                                                                                      <  2:1780331/84‑1 (MQ=255)

                                                                                                  cATCGTCAGCGACCTGCGCCTGCCCGGCACGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCaa                                                   >  2:1915889/1‑150 (MQ=255)

                                                                                                   aTCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGAGCGAGGTAGACTATATCTCCaaa                                                  >  2:2249128/1‑149 (MQ=255)

                                                                                                    tCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGc                                                 <  2:1282433/150‑1 (MQ=255)

                                                                                                       tCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGccc                                               <  1:1670694/149‑1 (MQ=255)

                                                                                                       tCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGccc                                               >  2:1670694/1‑149 (MQ=255)

                                                                                                       tCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGccc                                               >  1:809315/1‑149 (MQ=255)

                                                                                                       tCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGccc                                               <  2:809315/149‑1 (MQ=255)

                                                                                                       tCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCt                                              >  1:2152377/1‑150 (MQ=255)

                                                                                                       tCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCt                                              >  2:1570476/1‑150 (MQ=255)

                                                                                                            gACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGAc                                         <  1:1611924/150‑1 (MQ=255)

                                                                                                             aCCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGAcc                                        >  1:1489101/1‑150 (MQ=255)

                                                                                                               cTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGAt                                                                                                                           <  2:1772990/65‑1 (MQ=255)

                                                                                                               cTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGAt                                                                                                                           >  1:1772990/1‑65 (MQ=255)

                                                                                                               cTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGACCACGGTCGCGGTGGACTACATCGCCAAGCCCTTCGACCac                                      >  2:776667/1‑150 (MQ=255)

                                                                                                                 gcgcCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCacga                                    <  2:567256/150‑1 (MQ=255)

                                                                                                                 gcgcCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCacga                                    >  1:464913/1‑150 (MQ=255)

                                                                                                                    ccTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGa                                                                                  <  2:1223520/101‑1 (MQ=255)

                                                                                                                    ccTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGa                                                                                  >  1:1223520/1‑101 (MQ=255)

                                                                                                                      tGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTAc                                                                                                              >  1:2204588/1‑71 (MQ=255)

                                                                                                                      tGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTAc                                                                                                              <  2:2204588/71‑1 (MQ=255)

                                                                                                                      tGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGa                                                               >  2:1582746/1‑118 (MQ=255)

                                                                                                                      tGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGa                                                               <  1:1582746/118‑1 (MQ=255)

                                                                                                                       gCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGAt                              <  2:1770777/150‑1 (MQ=255)

                                                                                                                        cccGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACTCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGATTACATCGCCAAGCCCTTCGACCACGACGAGAtg                             <  1:776667/150‑1 (MQ=255)

                                                                                                                         ccGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTg                                                                                         <  1:711517/89‑1 (MQ=255)

                                                                                                                         ccGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTg                                                                                         >  2:711517/1‑89 (MQ=255)

                                                                                                                         ccGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGAtgc                            >  1:1763091/1‑150 (MQ=255)

                                                                                                                           ggCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGAtgctg                          <  2:252086/150‑1 (MQ=255)

                                                                                                                                 cccGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGATGCTgcaggca                    <  1:813967/150‑1 (MQ=255)

                                                                                                                                  ccGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGATGCTgcaggcag                   >  2:936599/1‑150 (MQ=255)

                                                                                                                                    ggCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGGCTGCGCTCAGCGGCCGCCTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACTACGAGATGCTGCAGGCAGTa                 <  1:1915889/150‑1 (MQ=255)

                                                                                                                                    ctggccGAGCTTGATC‑AGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCAcgacga                                 <  1:1992776/130‑1 (MQ=255)

                                                                                                                                    ctggccGAGCTTGATC‑AGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCAcgacga                                 >  2:1992776/5‑134 (MQ=255)

                                                                                                                                        ccGAGCTTGATC‑AGCTCGGTGCAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGt                                                                                          <  1:1208055/74‑1 (MQ=25)

                                                                                                                                         cGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGcc                                                     >  1:191628/1‑109 (MQ=255)

                                                                                                                                         cGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGcc                                                     <  2:191628/109‑1 (MQ=255)

                                                                                                                                           aGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTa                                                            <  1:849378/100‑1 (MQ=255)

                                                                                                                                           aGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTa                                                            >  2:849378/1‑100 (MQ=255)

                                                                                                                                           aGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGATGCTGCAGGCAGTAGCGCGTa          <  2:1589200/150‑1 (MQ=255)

                                                                                                                                           aGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCACTTACGCCAGCCTGCGCTCAGCGGTGGACTCCGTGAAAATCGGCGCGGTGCGTCGCTGCCCTGAGCGGTGCGACCACGACGAGATGCTGCAGGCAGTAGCGCGTa          <  1:43516/150‑1 (MQ=255)

                                                                                                                                            gcttGATC‑AGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTc                                            >  2:1334448/4‑115 (MQ=255)

                                                                                                                                            gcttGATC‑AGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGATAAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTc                                            <  1:1334448/112‑1 (MQ=255)

                                                                                                                                                gATCAAGCTCGGC‑CAGGGCACCCCGGTGCTTATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCCAGCACTTCGACCACGACGAGATGGTGCAGGCAGTAGCGCTTATTCt      >  1:1132652/1‑150 (MQ=255)

                                                                                                                                                 aTCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACgaga                               <  2:1698145/124‑1 (MQ=255)

                                                                                                                                                 aTCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACgaga                               >  1:1698145/1‑124 (MQ=255)

                                                                                                                                                 aTCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGATGCTGCAGGCAGTAGCGCGTATTCTg     >  2:1056320/1‑150 (MQ=255)

                                                                                                                                                   cAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGATGCTGCAGGCAGTAGCGCGTATTCTGCg   <  1:2249128/150‑1 (MQ=255)

                                                                                                                                                    aaGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGATGCTGCAGGCAGTAGCGCGTCTTGTGCGt  >  1:347778/1‑150 (MQ=255)

                                                                                                                                                    aaGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGAGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGATGCTGCAGGCAGTACCGCGTATTCTGGGt  >  2:171783/1‑150 (MQ=255)

GCCCTGCGTCGCCTGCTTGAGCGGAACCAGTACCAGGTCAGCGAGGCCGGCTCGGTGCAGGAAGCCCAGGAACGCTTCAGCATTGCCACTTTCGACCTCATCGTCAGCGACCTGCGCCTGCCCGGCGCGCCCGGCACCGAGC‑TGATCAAGCTCGGC‑CAGGGCACCCCGGTGCTGATCATGACCAGTTACGCCAGCCTGCGCTCAGCGGTGGACTCGATGAAAATGGGCGCGGTGGACTACATCGCCAAGCCCTTCGACCACGACGAGATGCTGCAGGCAGTAGCGCGTATTCTGCGT  >  NC_002947/5336644‑5336940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`