Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	850,452:1	+G	8.9%	intergenic (‑32/‑773)	pcs ← / → hemA	phosphatidylcholine synthase/glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	850,452	1	.	G	8.9%	203.3 / 16.2	56	intergenic (‑32/‑773)	pcs/hemA	phosphatidylcholine synthase/glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base . (28/23);  new base G (2/3);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

TAAATATAGTAGGAAAAATAAATATAGCTCAGCGTAGAGGCCGAAATAGCACACAAGTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTC  >  NC_002947/850311‑850600                                                                                                                                               |                                                                                                                                                     tAAATATAGTAGGAAAAATAAATATAGCTCAGCGTAGAGGCCGAAATAGCACACAAGTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGc                                                                                                                                              <  1:2050568/150‑1 (MQ=255)     tataGTAGGAAAAATAAATATAGCTCAGCGTAGAGGCCGAAATAGCACACAAGTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATAc                                                                                                                                          <  1:691754/150‑1 (MQ=255)                   taaataTAGCTCAGCGTAGAGGCCGAAATAGCACACAAGTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAAt                                                                                                                            >  2:1590011/1‑150 (MQ=255)                        ataGCTCAGCGTAGAGGCCGAAATAGCACACAAGTTAACGCCCCAAAGAAGCACTTAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGttt                                                                                                                       <  1:82243/150‑1 (MQ=255)                            cTCAGCGTAGAGGCCGAAATAGCACACAAGTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTaa                                                                                                                   >  1:1285516/1‑150 (MQ=255)                                 cGTAGAGGCCGAAATAGCACACAAGTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATaa                                                                                                              <  2:2219371/150‑1 (MQ=255)                                     gagGCCGAAATAGCACACAAGTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCtt                                                                                                          <  2:2324838/150‑1 (MQ=255)                                        gCCGAAATAGCACACAAGTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGaa                                                                                                                                                <  1:1586626/109‑1 (MQ=255)                                        gCCGAAATAGCACACAAGTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGaa                                                                                                                                                >  2:1586626/1‑109 (MQ=255)                                          cGAAATAGCACACAAGTTAACGACCAAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCGTGCATTGGAAATCGCTTTTAAGATAAGCTTAACac                                                                                                     >  2:862081/1‑149 (MQ=255)                                              aTAGCACACAAGTTAACGCCCCAAATAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACt                                                                                                 <  1:79470/150‑1 (MQ=255)                                                 gCACACAAGTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGAATACACTACAATGGAAATCGTTTTTAAGATAAGCATAACAAAACTGaa                                                                                              >  1:1352537/1‑150 (MQ=255)                                                         gTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATaaaa                                                                                      >  2:2102860/1‑150 (MQ=255)                                                                 cccAAAGAAGCACTGAGTGCCTACTAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGcttct                                                                              >  2:1236396/1‑150 (MQ=255)                                                                   cAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCAATG‑CACGAAGCATAACCTGAATTAGAAATCATTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCttg                                                                            >  1:1159027/1‑150 (MQ=255)                                                                      cctatctcTGAGTGCCTACGGGCTACTGCAGCTTGCTCCATTGCTCTCTCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCttgttg                                                                         <  2:613080/143‑1 (MQ=255)                                                                      agaagCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCttgttg                                                                         <  2:1285516/150‑1 (MQ=255)                                                                           cACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATATCCTGCATTGGAAATCATTTTTAAGATAAGATTAACAATTCTGATATATAAAAATGCTTCTTGTTGgtgtg                                                                    >  1:1905550/1‑150 (MQ=255)                                                                              tGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTCATAAGACTGAAATATAAAAATGCTTCTTGTTggagatcgg                                                                 >  1:613080/1‑143 (MQ=255)                                                                                  tGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATCTGGAAATGCTTCTTGTTGGTGTGTCACCaa                                                             >  1:1756721/1‑150 (MQ=255)                                                                                  tGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCaa                                                             <  1:1164160/150‑1 (MQ=255)                                                                                   gCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCaag                                                            <  1:2102860/150‑1 (MQ=255)                                                                                     cTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGAATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAAAGCTTCTTGTTGGTGTGTCACCaagta                                                          >  1:1522999/1‑150 (MQ=255)                                                                                        cGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCt                                                                                                           >  1:916236/1‑98 (MQ=255)                                                                                        cGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCt                                                                                                           <  2:916236/98‑1 (MQ=255)                                                                                          agaTACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAgtgt                                                     <  2:855492/150‑1 (MQ=255)                                                                                           gaTACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGcttc                                                                               <  1:607148/123‑1 (MQ=255)                                                                                           gaTACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGcttc                                                                               >  2:607148/1‑123 (MQ=255)                                                                                            aTACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGc                                                   <  2:29371/150‑1 (MQ=255)                                                                                              aCTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGcat                                                 <  1:704871/150‑1 (MQ=255)                                                                                                tGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGcatca                                               <  1:1236396/150‑1 (MQ=255)                                                                                                 gCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAACGTGGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGcatcat                                              >  1:21869/1‑150 (MQ=255)                                                                                                   aaaTTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTc                                            >  1:1027079/1‑150 (MQ=255)                                                                                                   aaaTTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGAATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAAAGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTc                                            <  2:1522999/150‑1 (MQ=255)                                                                                                           aaacgatttccaatgcagggtatGCTTCGTGC‑ATGGCAGGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTtcat                                    <  1:891846‑M1/127‑1 (MQ=255)                                                                                                            cATTGCTCGCGCCTATTATATAGATTAATGGCATG‑CAGGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTtcatc                                   <  2:21869/150‑1 (MQ=255)                                                                                                               tGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTaa                                                                                                        >  2:1581685/1‑78 (MQ=255)                                                                                                               tGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTaa                                                                                                        <  1:1581685/78‑1 (MQ=255)                                                                                                                 cTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTAc                              >  1:1418175/1‑150 (MQ=255)                                                                                                                  tCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGcttc                                                                               >  2:1163682/1‑100 (MQ=255)                                                                                                                  tCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGcttc                                                                               <  1:1163682/100‑1 (MQ=255)                                                                                                                    ccaatgcagggtatGCTTCGTGC‑ATGGCAGGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTc                                            >  2:754693‑M1/15‑133 (MQ=255)                                                                                                                    ccaatgcagggtatGCTTCGTGC‑ATGGCAGGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCGTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTc                                            <  1:754693‑M1/119‑1 (MQ=255)                                                                                                                      gcCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCgctg                         >  2:1363430/1‑150 (MQ=255)                                                                                                                       atgcagggtatGCTTCGTGC‑ATGGCAGGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCgctgc                        >  2:891846‑M1/12‑150 (MQ=255)                                                                                                                           ttatATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTaca                    >  2:762315/1‑148 (MQ=255)                                                                                                                             atataGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCgctgc                        >  2:1158885/1‑144 (MQ=255)                                                                                                                             atataGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCgctgc                        <  1:1158885/144‑1 (MQ=255)                                                                                                                             atataGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAAcc                  >  2:1555070/1‑150 (MQ=255)                                                                                                                              gtatGCTTCGTGC‑ATGGCAGGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCt                 <  2:663758‑M1/146‑1 (MQ=255)                                                                                                                                 aGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCtcat              >  2:38586/1‑150 (MQ=255)                                                                                                                                  GCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATGAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCtcatc             >  1:152096‑M1/1‑150 (MQ=255)                                                                                                                                    TTCCTGCCATG‑AACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGAAAAGAATAACAGTACGGAGGTAGAGAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCaa           >  1:862081‑M1/1‑150 (MQ=255)                                                                                                                                         gCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATaaaa      <  2:1905550/150‑1 (MQ=255)                                                                                                                                            aTG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACtt   >  1:705056/1‑150 (MQ=255)                                                                                                                                             tG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAAAAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTc  >  1:1698437/1‑150 (MQ=255)                                                                                                                                               |                                                                                                                                                     TAAATATAGTAGGAAAAATAAATATAGCTCAGCGTAGAGGCCGAAATAGCACACAAGTTAACGCCCCAAAGAAGCACTGAGTGCCTACGAGATACTGCAAATTCTTCCATTGCTCGCGCCTATTATATAGCTTCCTGCCATG‑CACGAAGCATACCCTGCATTGGAAATCGTTTTTAAGATAAGCTTAACAAAACTGAAATATAAAAATGCTTCTTGTTGGTGTGTCACCAAGTAAGTGTGCATCATTCCAGTTCATCATTACCGCTGCTAAACCTCATCAATAAAACTTC  >  NC_002947/850311‑850600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑