Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,464,205	T→A	6.4%	T474S (ACC→TCC) ‡	algX ←	alginate biosynthesis protein AlgX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,464,205	0	T	A	6.4%	268.8 / 10.2	94	T474S (ACC→TCC) ‡	algX	alginate biosynthesis protein AlgX
Reads supporting (aligned to +/- strand):  ref base T (44/43);  new base A (3/3);  total (47/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.46e-01

TTGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTCG  >  NC_002947/1464058‑1464353                                                                                                                                                     |                                                                                                                                                     ttGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCt                                                                                                                                                    <  2:2294490/150‑1 (MQ=255)      cTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCa                                                                                                                                               <  1:1140566/150‑1 (MQ=255)      cTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCa                                                                                                                                               >  2:1331676/1‑150 (MQ=255)        tttCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAgg                                                                                                                                             <  1:74031/150‑1 (MQ=255)           cGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCAGACCGCGCAGGCag                                                                                                                                          <  1:358694/150‑2 (MQ=255)            gATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGAGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACg                                                                                                                                         >  1:853274/1‑150 (MQ=255)             aTCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCGTGCCTGCGCAGACCGCGCAGGCagg                                                                                                                                         <  2:2316482/150‑3 (MQ=255)                 ccTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGaa                                                                                                                                    >  2:1364721/1‑150 (MQ=255)                 ccTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGaa                                                                                                                                    <  2:2173125/150‑1 (MQ=255)                 ccTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGGCTGCGCAGGCACGGCGaa                                                                                                                                    <  1:1241472/150‑1 (MQ=255)                 ccTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTCGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGaa                                                                                                                                    <  1:1331676/150‑1 (MQ=255)                     gtagtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttg                                                                                                                                >  1:1439606/1‑150 (MQ=255)                     gtagtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCAGCCCTGTCC‑TGCCTACGCGGTCTGCGCAGTCACGGCGAAgttg                                                                                                                                >  2:612674/1‑150 (MQ=255)                      tagtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCCACACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttgt                                                                                                                               <  1:612674/150‑1 (MQ=255)                       agtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttgtt                                                                                                                              <  1:1052996/150‑1 (MQ=255)                        gtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGttgttg                                                                                                                             >  1:92399/1‑150 (MQ=255)                            cccTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGtt                                                                                                                         <  2:1418441/150‑1 (MQ=255)                             ccTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGttt                                                                                                                        >  1:1849979/1‑150 (MQ=255)                             ccTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGttt                                                                                                                        <  1:1940090/150‑1 (MQ=255)                              cTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTg                                                                                                                       <  2:1304036/150‑1 (MQ=255)                              cTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTg                                                                                                                       >  1:836520/1‑150 (MQ=255)                              cTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTg                                                                                                                       >  1:2250748/1‑150 (MQ=255)                                gcggcgGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCa                                                                                                                     <  2:1987895/150‑1 (MQ=255)                                gcggcgGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCa                                                                                                                     <  2:2326436/150‑1 (MQ=255)                                     ggTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCt                                                                                                                <  2:836520/150‑1 (MQ=255)                                        aCCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTgg                                                                                                             >  2:1916157/1‑150 (MQ=255)                                         ccAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGc                                                                                                            <  1:1916157/150‑1 (MQ=255)                                           aGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCtt                                                                                                          <  2:1865894/150‑1 (MQ=255)                                           aGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCtt                                                                                                          <  1:2020475/150‑1 (MQ=255)                                                   gTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTc                                                                                                  <  2:1392588/150‑1 (MQ=255)                                                      cgcgGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTgg                                                                                               <  2:1849979/150‑1 (MQ=255)                                                               accaAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGc                                                                                                                            >  2:1820642/1‑112 (MQ=255)                                                               accaAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGc                                                                                                                            <  1:1820642/112‑1 (MQ=255)                                                                 caAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTc                                                                                    >  1:491056/1‑150 (MQ=255)                                                                  aaaCAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCGGCGCAGTCACGGCGAAGTTGTTGCGTTTGCAAACATTGGCTTCGTCCTGCTGGGTGCCGCTTTcc                                                                                   >  1:1287959/1‑150 (MQ=255)                                                                    aCAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCa                                                                                                                     <  1:2287906/114‑1 (MQ=255)                                                                    aCAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCa                                                                                                                     >  2:2287906/1‑114 (MQ=255)                                                                      aaGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCAGACCGCGCAGGCa                                                                                                                                           <  1:2171062/90‑1 (MQ=255)                                                                      aaGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCAGACCGCGCAGGCa                                                                                                                                           >  2:2171062/1‑90 (MQ=255)                                                                       agcccAACGCGCGCAGGGCGGGGCTGGAAATTCGTTTCCGCCGTGTCATGCGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGccc                                                                              <  1:23217/147‑1 (MQ=255)                                                                            aaCGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTa                                                                         >  1:6577/1‑150 (MQ=255)                                                                            aaCGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTa                                                                         >  2:726952/1‑150 (MQ=255)                                                                            aaCGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTa                                                                         >  1:1660037/1‑150 (MQ=255)                                                                                       ggCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCt                                                                                                           >  1:25827/1‑105 (MQ=255)                                                                                       ggCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCt                                                                                                           <  2:25827/105‑1 (MQ=255)                                                                                       ggCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGCAcgcg                                                              >  1:951034/1‑150 (MQ=255)                                                                                        gCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGa                                                             <  2:491056/150‑1 (MQ=255)                                                                                              cTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGgc                                                       <  1:726952/150‑1 (MQ=255)                                                                                               tGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTTGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCg                                                                                  <  2:71112/122‑1 (MQ=255)                                                                                               tGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCg                                                                                  >  1:71112/1‑122 (MQ=255)                                                                                               tGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTa                                                                         <  2:1785266/131‑1 (MQ=255)                                                                                               tGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTa                                                                         >  1:1785266/1‑131 (MQ=255)                                                                                               tGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGgcg                                                      >  1:1830327/1‑150 (MQ=255)                                                                                                 gAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgct                                                    >  2:63818/1‑150 (MQ=255)                                                                                                 gAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgct                                                    >  2:567379/1‑150 (MQ=255)                                                                                                   aaTTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgctgg                                                  <  2:92399/150‑1 (MQ=255)                                                                                                     ttCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgctggct                                                >  1:1155881/1‑150 (MQ=255)                                                                                                       cGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGctggctgg                                              <  2:1660037/150‑1 (MQ=255)                                                                                                         tttGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGaa                                                                                                                                    >  1:90091/1‑62 (MQ=255)                                                                                                         tttGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGcc                                            <  1:567379/150‑1 (MQ=255)                                                                                                         tttGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGcc                                            >  2:897735/1‑150 (MQ=255)                                                                                                         tttGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGcc                                            >  1:1472667/1‑150 (MQ=255)                                                                                                         tttGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCCaa                                                                                                                                    <  2:90091/62‑1 (MQ=255)                                                                                                               aaGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTcc                                      >  2:219464/1‑150 (MQ=255)                                                                                                                 gggTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgctggctg                                               >  2:354832/1‑139 (MQ=255)                                                                                                                 gggTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgctggctg                                               <  1:354832/139‑1 (MQ=255)                                                                                                                 gggTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTc                                    <  1:219464/150‑1 (MQ=255)                                                                                                                      aTGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGa                                                             >  2:906353/1‑120 (MQ=255)                                                                                                                      aTGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGa                                                             <  1:906353/120‑1 (MQ=255)                                                                                                                       tGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTc                              <  1:63818/150‑1 (MQ=255)                                                                                                                        gggTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttg                                                                                                                                >  1:661262/1‑51 (MQ=255)                                                                                                                        gggTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttg                                                                                                                                <  2:661262/51‑1 (MQ=255)                                                                                                                         ggTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGa                                                                                                       >  2:1302407/1‑75 (MQ=255)                                                                                                                         ggTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGa                                                                                                       <  1:1302407/75‑1 (MQ=255)                                                                                                                          gTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGGAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTcgcg                           >  1:1960667/1‑150 (MQ=255)                                                                                                                             gCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCAt                        >  1:904776/1‑150 (MQ=255)                                                                                                                               cTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCt                      >  2:432856/1‑150 (MQ=255)                                                                                                                                tCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGATTAGACCTTCTGGGTGCCGCTTTCCGGGCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTCCGCGCATCTg                     <  2:853274/150‑1 (MQ=255)                                                                                                                                 cACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTgg                    <  1:1364721/150‑1 (MQ=255)                                                                                                                                   ttcgccgtgcctgcGCAGACCGCGCAGGCAGGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGa                   >  2:9334/8‑150 (MQ=255)                                                                                                                                    tGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATc                       >  2:1159225/1‑144 (MQ=255)                                                                                                                                    tGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATc                       <  1:1159225/144‑1 (MQ=255)                                                                                                                                      gcc‑tgcctGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGAc               >  1:1982139/1‑150 (MQ=255)                                                                                                                                      gcc‑tgcctGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGAc               >  1:111651/1‑150 (MQ=255)                                                                                                                                       ccgtgcctgcGCAGACCGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGAc               >  1:493107/4‑150 (MQ=255)                                                                                                                                        c‑tgcctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTg                                                                                            >  2:1113112/1‑71 (MQ=255)                                                                                                                                        c‑tgcctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTg                                                                                            <  1:1113112/71‑1 (MQ=255)                                                                                                                                            cctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGc          >  2:1588072/1‑150 (MQ=255)                                                                                                                                              tgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCgg        >  1:1877330/1‑150 (MQ=255)                                                                                                                                               gcgcGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGTAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGc       >  2:1035018/1‑150 (MQ=255)                                                                                                                                                  cgGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGt    >  2:250635/1‑150 (MQ=255)                                                                                                                                                    gTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTCg  <  2:1287959/150‑1 (MQ=255)                                                                                                                                                    gTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTCg  <  2:1155881/150‑1 (MQ=255)                                                                                                                                                    gTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTCg  <  1:432856/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TTGAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAAATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCC‑TGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTCG  >  NC_002947/1464058‑1464353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑