Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,723,025 T→C 5.6% I539T (ATC→ACC)  PP_1517 → RND family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,723,0250TC 5.6% 206.3 / 2.2 74I539T (ATC→ACC) PP_1517RND family transporter
Reads supporting (aligned to +/- strand):  ref base T (39/30);  new base C (2/2);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.14e-01

CTACCCGACCTGGCCAAGCTGCATGCGGCGCGTCATGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAAGCAGCTGGAG  >  NC_002947/1722880‑1723173
                                                                                                                                                 |                                                                                                                                                    
cTACCCGACCTGGCCAAGCTGCATGCGGCGCGTCATGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCCGCGCAAGACGGTAATCCTGCTGACCATCGCt                                                                                                                                                  <  2:1438837/150‑1 (MQ=255)
    ccGACCTGGCCAAGCTGCATGCGGCGCGTCATTGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCt                                                                                                                                              >  1:1899879/1‑150 (MQ=255)
         cTGGCCAAGCTGCATGCGGCGCGTCATGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCACCGCTGCCTTTGTc                                                                                                                                         >  2:1765658/1‑150 (MQ=255)
          tGGCCAAGCTGCATGCGGCGCGTCATGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCg                                                                                                                                        >  1:2253576/1‑150 (MQ=255)
              cAAGCTGCATGCGGCGCGTCATGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCAt                                                                                                                                                      <  1:1327774/132‑1 (MQ=255)
              cAAGCTGCATGCGGCGCGTCATGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCAt                                                                                                                                                      >  2:1327774/1‑132 (MQ=255)
                  cTGCATGCGGCGCGTCATGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATc                                                                                                                                >  1:1466483/1‑150 (MQ=255)
                      aTGCGGCGCGTCATGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCctgc                                                                                                                            <  1:785266/150‑1 (MQ=255)
                           gcgcGTCATGGCAACGACGGCCATGCACCAGACCCAGACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCCAGACGGTAATCCTGCTCACCATCCCTGCCTTTGTCGGCAGCATCCTGCTGTTc                                                                                                                       <  2:277315/150‑1 (MQ=255)
                             gcgTCATGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCg                                                                                                                     >  2:2061179/1‑150 (MQ=255)
                               gTCATGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCt                                                                                                                   >  2:1915631/1‑150 (MQ=255)
                                  aTGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGt                                                                                                                                          >  2:1750584/1‑124 (MQ=255)
                                  aTGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGt                                                                                                                                          <  1:1750584/124‑1 (MQ=255)
                                   tGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGt                                                                                                               >  1:1626621/1‑150 (MQ=255)
                                     gCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGCAGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTCCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTAc                                                                                                             >  2:134827/1‑150 (MQ=255)
                                       aaGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTAccc                                                                                                           >  1:508680/1‑150 (MQ=255)
                                         ggACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCca                                                                                                         >  1:1565697/1‑150 (MQ=255)
                                          gACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCcag                                                                                                        <  2:1425494/150‑1 (MQ=255)
                                           ccGGCCATGCCCCAGTCCCACACGACTCCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCcagc                                                                                                       <  2:2253576/149‑1 (MQ=255)
                                           aCGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATc                                                                                                                                >  2:411523/1‑125 (MQ=255)
                                           aCGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATc                                                                                                                                <  1:411523/125‑1 (MQ=255)
                                              gCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGt                                                                                                    <  1:1783348/150‑1 (MQ=255)
                                               ccATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGtt                                                                                                   >  1:471924/1‑150 (MQ=255)
                                               ccATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTATCACCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGtt                                                                                                   >  1:122197/1‑150 (MQ=255)
                                                    cACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGGGCAAGAAGAAAGGTATCAACAAAATCGCCGCCGTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACGCCAGCAGTTCTTcc                                                                                              >  1:2280059/1‑150 (MQ=255)
                                                     aCCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTccc                                                                                             >  2:1540709/1‑150 (MQ=255)
                                                             cATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCg                                                                                     >  2:2046418/1‑150 (MQ=255)
                                                              aTACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCt                                                                                                                   >  2:1646898/1‑119 (MQ=255)
                                                              aTACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCt                                                                                                                   <  1:1646898/119‑1 (MQ=255)
                                                              aTACGCCACACCGTTGTGCGAGCGTATGCAGCGGCTTGTTGAGTGTTGCGTACGGCCGCGCAAGACGGTAATCCTGCTGCCCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCgg                                                                                    <  2:842499/150‑1 (MQ=255)
                                                                  gCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCACCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTc                                                                                                                 <  2:1844937/117‑1 (MQ=255)
                                                                  gCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCACCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTc                                                                                                                 >  1:1844937/1‑117 (MQ=255)
                                                                       cccGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATc                                                                                                                                >  1:1264360/1‑97 (MQ=255)
                                                                       cccGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATc                                                                                                                                <  2:1264360/97‑1 (MQ=255)
                                                                       cccGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGa                                                                           >  2:629775/1‑150 (MQ=255)
                                                                       cccGTTCTACCAGCGCGGGCGACCGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGa                                                                           <  2:1096208/150‑1 (MQ=255)
                                                                        ccGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCcagcag                                                                                                     <  1:2050388/123‑1 (MQ=255)
                                                                        ccGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCcagcag                                                                                                     >  2:2050388/1‑123 (MQ=255)
                                                                         cGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGc                                                                         <  1:1709859/150‑1 (MQ=255)
                                                                              tACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATg                                                                    <  2:2221605/150‑1 (MQ=255)
                                                                              tACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATg                                                                    >  1:783111/1‑150 (MQ=255)
                                                                               aCCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATgg                                                                   <  2:1683909/150‑1 (MQ=255)
                                                                                         gCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGaa                                                         <  1:2122964/150‑1 (MQ=255)
                                                                                              ggCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAGTCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTgg                                                    <  2:1894408/150‑1 (MQ=255)
                                                                                              ggCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTgg                                                    >  2:2039970/1‑150 (MQ=255)
                                                                                                 tggtggAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCGGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCg                                                 >  2:76707/1‑150 (MQ=255)
                                                                                                   gtggAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGcc                                                  >  2:1325228/1‑147 (MQ=255)
                                                                                                   gtggAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGcc                                                  <  1:1325228/147‑1 (MQ=255)
                                                                                                   gtagAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGATGACCTGAAGCTGGCCGaa                                               >  2:1540462/4‑150 (MQ=255)
                                                                                                      gAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCAAGGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGgc                                            <  1:134827/150‑1 (MQ=255)
                                                                                                        gtggtgCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTGTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGgcgc                                          <  2:1899879/150‑1 (MQ=255)
                                                                                                           gtgCGTACGGCGGCGCAAGACGGTAATCCTGCTGCCCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGACGAAGGCGCCTc                                       >  1:1615565/1‑150 (MQ=255)
                                                                                                           gtgCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCt                                                      <  1:1373687/135‑1 (MQ=255)
                                                                                                           gtgCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCt                                                      >  2:1373687/1‑135 (MQ=255)
                                                                                                           gtgCGCACCGCGGCGCAAGACGGTAATCCTCCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCCTCGTACCCCAGCAGGTCCTCCCGCCTTCCGGCCGCCCGGAGCCGATGGTTCACCTGAAGCTGGCCGAAGGCCCCTc                                       >  2:154034/1‑150 (MQ=255)
                                                                                                                      ggcgCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATa                            >  2:1996284/1‑150 (MQ=255)
                                                                                                                         gcAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATAccg                         >  1:369931/1‑150 (MQ=255)
                                                                                                                           aaGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATAgcgcc                       >  2:854093/1‑150 (MQ=255)
                                                                                                                           aaGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGCTCGTTCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATAccgcc                       >  1:157272/1‑150 (MQ=255)
                                                                                                                              aCGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTACCGGATACTCAGCGCCCGGAGCTGTTGGTTGACCTGAAGCTGGCCGAAGGCGAACCGCTGGCCAATACCGCCGAg                    >  1:735235/1‑150 (MQ=255)
                                                                                                                                ggTAATCCTGCTGACCATCGCTGCCTTTGTCGGACGTATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCg                  >  1:1714914/1‑150 (MQ=255)
                                                                                                                                 gTAATCCTGCTGACCACCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCgg                 <  1:1765658/150‑1 (MQ=255)
                                                                                                                                     tCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCa             >  2:1251180/1‑150 (MQ=255)
                                                                                                                                       ctgctgACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAag           <  2:369931/150‑1 (MQ=255)
                                                                                                                                       ctgctgACCATCGCTGCCTTTGTCGGCAGCACCCTGCGGTTCCGCTTCGTACCCCAGCAGTTGGTCCCGGCTGGCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAag           <  2:508680/150‑1 (MQ=255)
                                                                                                                                        tgctgACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTg                                   >  2:917678/1‑125 (MQ=255)
                                                                                                                                        tgctgACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTg                                   <  1:917678/125‑1 (MQ=255)
                                                                                                                                        tgctgACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAagc          <  1:629775/150‑1 (MQ=255)
                                                                                                                                        tgctgACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAaga          >  1:934708/1‑149 (MQ=255)
                                                                                                                                         gctgACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCGTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAagca         >  2:1308851/1‑150 (MQ=255)
                                                                                                                                           tgACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGATCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAagcagc       <  2:1714914/150‑1 (MQ=255)
                                                                                                                                             aCCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCCGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAAGCAGCTg     <  1:2046418/150‑1 (MQ=255)
                                                                                                                                             aCCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAAGCAGCTg     <  2:1466483/150‑1 (MQ=255)
                                                                                                                                             aCCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAAGCAGCTg     <  1:1540462/150‑1 (MQ=255)
                                                                                                                                               cATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAAGCAGCTGGa   >  2:2301737/1‑150 (MQ=255)
                                                                                                                                                aTCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAAGCAGCTGGAg  <  1:1251180/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CTACCCGACCTGGCCAAGCTGCATGCGGCGCGTCATGGCAAGGACGGCCATGCACCAGACCCATACGCCACCCCGTTCTACCAGCGCGTGCGACGGCTGGTGGAGTGGTGCGTACGGCGGCGCAAGACGGTAATCCTGCTGACCATCGCTGCCTTTGTCGGCAGCATCCTGCTGTTCCGCTTCGTACCCCAGCAGTTCTTCCCGGCTTCCGGGCGCCCGGAGCTGATGGTTGACCTGAAGCTGGCCGAAGGCGCCTCGCTGGCCAATACCGCCGAGCGGGTCAAGCAGCTGGAG  >  NC_002947/1722880‑1723173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑