Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,819,833 T→A 5.2% intergenic (+28/‑447) rpoS → / → PP_1624 RNA polymerase sigma S factor/group II intron‑encoding maturase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,819,8330TA 5.2% 411.2 / 10.0 132intergenic (+28/‑447)rpoS/PP_1624RNA polymerase sigma S factor/group II intron‑encoding maturase
Reads supporting (aligned to +/- strand):  ref base T (61/63);  new base A (3/4);  total (65/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 132 total aligned reads displayed.

TGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGTGTCG  >  NC_002947/1819685‑1819981
                                                                                                                                                     |                                                                                                                                                       
tGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGATATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTg                                                                                                                                                        >  1:285985/1‑150 (MQ=255)
 gTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGa                                                                                                                                                       >  2:814365/1‑150 (MQ=255)
       cTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑Cgg                                                                                                                                               <  1:717683/150‑1 (MQ=255)
         ggAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑Cgggg                                                                                                                                             >  2:83020/1‑150 (MQ=255)
             aTCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑tt                                                                                                                                        >  2:1120536/1‑150 (MQ=255)
              tCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCC‑GAGATCAC‑CGGGGCG‑ttt                                                                                                                                       <  2:1891662/150‑1 (MQ=255)
                 gCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCC‑GAGATCAC‑CGGGGCG‑tttttt                                                                                                                                    <  2:2307537/150‑2 (MQ=255)
                  ccTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑ttttt                                                                                                                                     <  2:2297334/148‑1 (MQ=255)
                  ccTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑ttttt                                                                                                                                     >  1:2297334/1‑148 (MQ=255)
                  ccTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTtgt                                                                                                                                   <  1:83020/150‑1 (MQ=255)
                   cTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTtgtg                                                                                                                                  >  2:1900062/1‑150 (MQ=255)
                     tatccGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAATAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTtgtgtg                                                                                                                                <  1:2220601/147‑1 (MQ=255)
                      aCCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTgc                                                                                                                               <  2:1628107/150‑1 (MQ=255)
                      aCCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTgc                                                                                                                               >  1:996963/1‑150 (MQ=255)
                          gTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCa                                                                                                                           <  1:137570/150‑1 (MQ=255)
                           tGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAg                                                                                                                          <  2:1318659/150‑1 (MQ=255)
                                gggTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATg                                                                                                                     <  1:2004171/150‑1 (MQ=255)
                                 ggTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCATGCCTgg                                                                                                                    <  1:2319563/150‑1 (MQ=255)
                                  gTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGgc                                                                                                                   >  1:682750/1‑150 (MQ=255)
                                        cAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTg                                                                                                             >  2:1356365/1‑150 (MQ=255)
                                         aaaTCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTgc                                                                                                            <  2:1555447/150‑1 (MQ=255)
                                           aTCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTgcgc                                                                                                          <  1:1215793/150‑1 (MQ=255)
                                            tCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGcgcg                                                                                                         >  2:2247008/1‑150 (MQ=255)
                                               aGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTAt                                                                                                      >  1:723417/1‑150 (MQ=255)
                                               aGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTAt                                                                                                      >  1:1431180/1‑150 (MQ=255)
                                               aGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTAt                                                                                                      <  2:1924510/150‑1 (MQ=255)
                                                 gTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATgc                                                                                                    <  1:2247008/150‑1 (MQ=255)
                                                      aGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAAc                                                                                               >  1:1485920/1‑150 (MQ=255)
                                                      aGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCCACCCGCTGTTCCATTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAAc                                                                                               <  1:1687801/150‑1 (MQ=38)
                                                       gggCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑ttttt                                                                                                                                     <  2:1445604/111‑1 (MQ=255)
                                                       gggCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑ttttt                                                                                                                                     >  1:1445604/1‑111 (MQ=255)
                                                        ggCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAcccc                                                                                             <  2:682750/150‑1 (MQ=255)
                                                         gCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCg                                                                                            >  1:2078154/1‑150 (MQ=255)
                                                         gCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGACCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCg                                                                                            >  1:1741238/1‑150 (MQ=255)
                                                          cTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGc                                                                                           <  2:1997817/150‑1 (MQ=255)
                                                             aaGCGCTTGCGCGAGCTCCTTGCAATGACGCGTTCTG‑CCAGCTATTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTg                                                                                        <  2:255498/150‑1 (MQ=25)
                                                             aaGCGCTTGCGCGAGATCCTTGGCCAGC‑CTGGATGCTCGGGTCAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTg                                                                                        <  2:1776364/150‑1 (MQ=25)
                                                             aaGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTg                                                                                        >  2:851413/1‑150 (MQ=255)
                                                                  cTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgt                                                                                   <  1:1367961/150‑1 (MQ=255)
                                                                        cgAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGc                                                                             <  1:893108/150‑1 (MQ=255)
                                                                          agaTCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTg                                                                                        <  1:577197/137‑1 (MQ=255)
                                                                             tCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGt                                                                        <  2:1872822/150‑1 (MQ=255)
                                                                              ccTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTg                                                                       >  2:1587354/1‑150 (MQ=255)
                                                                               cTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGTTTGTGGTGGCCAAGTgg                                                                      <  2:2078154/150‑1 (MQ=255)
                                                                                ttGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg                                                                                  >  2:1810272/1‑137 (MQ=255)
                                                                                ttGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTtgtg                                                                                  <  1:1810272/137‑1 (MQ=255)
                                                                                ttGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTggg                                                                     >  2:632704/1‑150 (MQ=255)
                                                                                ttGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGGGAGG‑T‑CGGGGAG‑ATATCGCGAGCTCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGGGTGGGGTAAGTggg                                                                     >  1:165875/1‑150 (MQ=25)
                                                                                 tGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                                    <  1:720556/150‑1 (MQ=255)
                                                                                  gAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTg                                                                   >  1:40684/1‑150 (MQ=255)
                                                                                   aaaaGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGTAACCCGCTCCGCCGCGGTGGCTAAGTGGGTgc                                                                  >  2:382539/1‑149 (MQ=37)
                                                                                    aaaGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                                    <  2:285559/147‑1 (MQ=255)
                                                                                    aaaGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGt                                                                    >  1:285559/1‑147 (MQ=255)
                                                                                     aaGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACt                                                                >  1:2137271/1‑150 (MQ=255)
                                                                                        a‑aTGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCACGGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTgg                                                             >  2:317922/1‑150 (MQ=255)
                                                                                          aTGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGa                                                            <  1:814365/150‑1 (MQ=255)
                                                                                              tCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCaa                                                                          >  1:2293293/1‑132 (MQ=255)
                                                                                                 gTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                                     <  1:1498085/150‑1 (MQ=255)
                                                                                                  tCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCATGCCTGgcgcac                                                                                                               <  2:403213/91‑3 (MQ=255)
                                                                                                  tCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCATGCCTGgcgcac                                                                                                               >  1:403213/1‑89 (MQ=255)
                                                                                                    cAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTc                                                  <  2:1706285/150‑1 (MQ=255)
                                                                                                     aGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                                     >  2:1440093/1‑146 (MQ=255)
                                                                                                     aGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                                     <  1:1440093/146‑1 (MQ=255)
                                                                                                       cGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCctc                                               <  1:847733/150‑1 (MQ=255)
                                                                                                             aTTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑Cggg                                                                                                                                              <  2:1329244/50‑1 (MQ=255)
                                                                                                             aTTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑Cggg                                                                                                                                              >  1:1329244/1‑50 (MQ=255)
                                                                                                             aTTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTgtggtg                                                                               >  2:2131857/1‑112 (MQ=255)
                                                                                                             aTTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTgtggtg                                                                               <  1:2131857/112‑1 (MQ=255)
                                                                                                              ttGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTacacac                                        >  1:608499/1‑150 (MQ=255)
                                                                                                                    cAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCa                                  >  2:1393765/1‑150 (MQ=255)
                                                                                                                         gctagcGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                                     <  2:921432/126‑1 (MQ=255)
                                                                                                                         gctagcGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGaaa                                                     >  1:921432/1‑126 (MQ=255)
                                                                                                                         gctagcGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAgggg                             >  2:48310/1‑150 (MQ=255)
                                                                                                                             gcgcTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAgt                         <  1:1120536/150‑1 (MQ=255)
                                                                                                                               ggtGTAACAAAAGACGCCCGGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAgtgt                       <  2:723417/148‑1 (MQ=255)
                                                                                                                                 tGTAACAAAAAACGCCCCGGTGATC‑T‑CGTGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCATACGCGCAAcgc                                                                                             <  2:1736727/78‑3 (MQ=21)
                                                                                                                                 tGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTaca                                           <  1:1990026/128‑1 (MQ=255)
                                                                                                                                 tGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCATACGCGCAAcgc                                                                                             >  1:1736727/1‑76 (MQ=25)
                                                                                                                                   tAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAgg                               >  2:891588/1‑138 (MQ=255)
                                                                                                                                   tAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAACTCCTCTACACACCCGGCAAgg                               <  1:891588/138‑1 (MQ=255)
                                                                                                                                    acacaaaaaCGCCCC‑GAGATC‑ACCGGGGCGTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGt                                                         >  2:244322/5‑112 (MQ=11)
                                                                                                                                    aaCAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATTCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGcc                  >  2:2312106/1‑150 (MQ=255)
                                                                                                                                     aCAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTc                                                  >  2:463224/1‑117 (MQ=255)
                                                                                                                                       aaaaacCGCCCCGGTGTTT‑T‑CCGTGCG‑TTTTTGTGTCCCCCATGCATGGCGCCTTGCGCGTATGCTCAACCCGCTTGGTTGTGGTGGCCAATTGGGTCACTTGGGGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGa               <  2:1035747/150‑1 (MQ=11)
                                                                                                                                       aaaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCctc                                               <  1:2206470/118‑1 (MQ=255)
                                                                                                                                       aaaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCctc                                               >  2:2206470/1‑118 (MQ=255)
                                                                                                                                       aaaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGa               <  1:382539/150‑1 (MQ=255)
                                                                                                                                       aaaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGCCAAGGGGAAGTGTTAGCCGGa               >  2:42347/1‑150 (MQ=255)
                                                                                                                                        aaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAg              <  2:608499/150‑1 (MQ=255)
                                                                                                                                        aaaaaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAg              <  1:1356365/150‑1 (MQ=255)
                                                                                                                                        aaaaaCGCCCC‑GAGATCAC‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAg              >  1:1379764/1‑150 (MQ=11)
                                                                                                                                        aaaaaCGCCCC‑GAGATC‑ACCGGGGCGTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAgt                         <  1:1518672/140‑1 (MQ=11)
                                                                                                                                        aaaaaCGCCCC‑GAGATC‑ACCGGGGCGTTTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAgt                         >  2:1518672/1‑140 (MQ=11)
                                                                                                                                           aaCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCa           >  1:244490/1‑150 (MQ=255)
                                                                                                                                            aCGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCaa          >  1:948238/1‑150 (MQ=255)
                                                                                                                                             cGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAg         <  1:1587354/150‑1 (MQ=255)
                                                                                                                                                cccGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGgt      >  2:200501/1‑150 (MQ=255)
                                                                                                                                                  cGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTg                                                        <  1:2136634/98‑1 (MQ=255)
                                                                                                                                                  cGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTg                                                        >  2:2136634/1‑98 (MQ=255)
                                                                                                                                                    gTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGTGTcg  >  1:2219560/1‑150 (MQ=39)
                                                                                                                                                     |                                                                                                                                                       
TGTTGGGCTGGAGATCGGCCTTACCCGTGAACGGGTGCGGCAAATCCAGGTGGAAGGGCTCAAGCGCTTGCGCGAGATCCTTGAAAAGA‑ATGGTCTGTCCAGCGAGTCATTGTTCCAGTAGCTAGCGCTGTAACAAAAAACGCCCCGGTGATC‑T‑CGGGGCG‑TTTTTGTGTGCGCCAGGCATGGCGCGTTGCGCGTATGCGCAACCCGCTTGGTTGTGGTGGCCAAGTGGGTGACTTGGAGGTGAAAGTCCTCTACACACCCGGCAAGGGGAAGTGTTAGCCGGAGGCAAGGGTGTCG  >  NC_002947/1819685‑1819981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑