Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,796,569	A→T	5.1%	noncoding (36/77 nt)	PP_t42 →	tRNA‑Val

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,796,569	0	A	T	5.1%	237.5 / 2.8	78	noncoding (36/77 nt)	PP_t42	tRNA‑Val
Reads supporting (aligned to +/- strand):  ref base A (37/37);  new base T (2/2);  total (39/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

TTTACGGGGCCCCCCTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAA  >  NC_002947/2796420‑2796712                                                                                                                                                      |                                                                                                                                                tttACGGGGCCCCCCTCAGTCAGCCTCTCTATAAAGAGTTTAAGCTTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGa                                                                                                                                                 >  1:436656/1‑150 (MQ=255) tttACGGGGCCCCCCTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGa                                                                                                                                                 >  1:110864/1‑150 (MQ=255) tttACGGGGCCCCCCTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGa                                                                                                                                                 >  1:986840/1‑150 (MQ=255) tttACGGGGCCCCCCTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGa                                                                                                                                                 >  1:1610270/1‑150 (MQ=255)    aCGGGGCCCCCCTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACAt                                                                                                                                              >  1:1334217/1‑150 (MQ=255)      ggggCCCCCCTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTTTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACAtgg                                                                                                                                            >  1:2154689/1‑150 (MQ=255)      ggggCCCCCCTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACAtgg                                                                                                                                            >  1:1549828/1‑150 (MQ=255)           cccccTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTgggg                                                                                                                                       <  2:986840/150‑1 (MQ=255)            ccccTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTggggg                                                                                                                                      >  1:370878/1‑150 (MQ=255)            ccccTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTggggg                                                                                                                                      <  1:1837531/150‑1 (MQ=255)              cgtcagtcagCCTCTCTATAAAGAGTTTAAGATGCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACCGGTGACATGTGGTTAGAGCACCACCTTGACATGGTGGGGGTc                                                                                                                                    >  1:1288194/3‑150 (MQ=38)               cTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCg                                                                                                                                   >  1:677819/1‑150 (MQ=255)                tcagtcagCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGt                                                                                                                                  >  2:1926801/1‑150 (MQ=255)                  agtcagCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTg                                                                                                                                <  1:1316430/150‑1 (MQ=255)                  agtcagCCGCTCTATAAAGAGTTTAAGATTCAGTAGTTTAGCGCGAAAAAAAGTGTTCACAGGGTGTTTGTAATGCCTAGAACGCGCACCACACGACAGCCACATAGCTCAGTTGGTTAAAGCACCACCTTGACATGGTGGGGGTCGTTg                                                                                                                                <  1:216121/150‑1 (MQ=25)                    tcagCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTgggg                                                                                                                                       >  2:1798639/1‑141 (MQ=255)                    tcagCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTgggg                                                                                                                                       <  1:1798639/141‑1 (MQ=255)                    tcagCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGt                                                                                                                                  >  2:1126064/1‑146 (MQ=255)                    tcagCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGt                                                                                                                                  <  1:1126064/146‑1 (MQ=255)                        ccTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGa                                                                                                                          <  1:660369/150‑1 (MQ=255)                        ccTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTGGGTTCGa                                                                                                                          >  2:668927/1‑150 (MQ=255)                                  aaGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATtgt                                                                                                                <  2:2056167/150‑1 (MQ=255)                                   agagTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGt                                                                                                                                  <  1:2123337/131‑1 (MQ=255)                                   agagTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGt                                                                                                                                  >  2:2123337/1‑131 (MQ=255)                                   agagTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTg                                                                                                                                <  2:1152548/133‑1 (MQ=255)                                   agagTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTg                                                                                                                                >  1:1152548/1‑133 (MQ=255)                                    gagTTTAAGATTCATTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGc                                                                                                              <  2:1334217/150‑1 (MQ=255)                                       tttAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTa                                                                                                           >  1:508298/1‑150 (MQ=255)                                        ttAAGATTCAGTAGGTTAGCGCTAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTTGGGGTCGTTGGTTCGAGTCCAATTGTGCCTaa                                                                                                          <  2:34178/150‑2 (MQ=255)                                             aTTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGAc                                                                                                                                                <  2:2118323/107‑1 (MQ=255)                                             aTTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGAc                                                                                                                                                >  1:2118323/1‑107 (MQ=255)                                             aTTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAAt                                                                                                     >  2:2059785/1‑150 (MQ=255)                                                 aGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAAtgaaa                                                                                                 >  2:2296454/1‑146 (MQ=255)                                                       ttAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAggg                                                                                           <  2:110864/150‑1 (MQ=255)                                                        tAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTAAGAGCACCACATCGAAAGTGTAGCCCTCGTGTATTCGAGTCCAATTGTGCCTACCAAATCGAAAAAgggg                                                                                          <  1:635480/150‑1 (MQ=18)                                                             cgAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGaaaa                                                                                               >  2:844637/1‑140 (MQ=255)                                                             cgAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGaaaa                                                                                               <  1:844637/140‑1 (MQ=255)                                                              gAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATcc                                                                                    <  1:976529/150‑1 (MQ=255)                                                                 aaaaaGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGt                                                                                                                                  >  1:465606/1‑101 (MQ=255)                                                                 aaaaaGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGt                                                                                                                                  <  2:465606/101‑1 (MQ=255)                                                                  aaaaGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGaaa                                                                                >  2:267580/1‑150 (MQ=255)                                                                    aaGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGtt                                                                                                                             <  2:1183800/103‑1 (MQ=255)                                                                    aaGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGtt                                                                                                                             >  1:1183800/1‑103 (MQ=255)                                                                    aaGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAgg                                                                              <  2:677819/150‑1 (MQ=255)                                                                       tgtTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTGGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTc                                                                           >  1:1195449/1‑150 (MQ=255)                                                                         ttGACAGTGTTTTTGTAATGCGTACAATGCATACCACTCGACAGGCTCATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCAc                                                                         <  2:1549828/150‑1 (MQ=18)                                                                         ttGACAGGGTTTTTGTAATGCGTAGAATGCGCCCCATACGACAGGAACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCAc                                                                         <  1:321962/150‑1 (MQ=255)                                                                         ttGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCAc                                                                         >  1:1115208/1‑150 (MQ=255)                                                                            aCAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCaa                                                                                                                    <  1:422604/104‑1 (MQ=255)                                                                            aCAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCaa                                                                                                                    >  2:422604/1‑104 (MQ=255)                                                                                ggTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCtttt                                                                  >  1:1007812/1‑150 (MQ=255)                                                                                 gTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCttttt                                                                 >  1:1331114/1‑150 (MQ=255)                                                                                      tGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTc                                                                                                                            >  2:430472/1‑86 (MQ=255)                                                                                      tGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTc                                                                                                                            <  1:430472/86‑1 (MQ=255)                                                                                        tAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGt                                                          <  2:436656/150‑1 (MQ=255)                                                                                          aTGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTcc                                                        >  2:640357/1‑150 (MQ=255)                                                                                              gTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTg                                                    <  1:267580/150‑1 (MQ=255)                                                                                              gTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTg                                                    <  2:2125830/150‑1 (MQ=255)                                                                                                  aaTGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTAGGTGCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCcgc                                                >  2:269663/1‑150 (MQ=255)                                                                                                              acGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAAt                                    >  2:796470/1‑150 (MQ=255)                                                                                                              acGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCCAGTCCAATTGTGCCTACCAAATCCAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATCCCCGCTCGCGCGGCAAt                                    <  2:1610270/150‑1 (MQ=18)                                                                                                                  cAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACcg                                <  1:796470/150‑1 (MQ=255)                                                                                                                  cAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACcg                                <  1:2059785/150‑1 (MQ=255)                                                                                                                    ggCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACcgcg                              <  2:1331114/150‑1 (MQ=255)                                                                                                                     gCACATAGCCCAGTTGGTTAGAGCACCACCTTGACAGGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGCTGATCCGAAAGAGTCACGCCTTTTTTCATTGTCCATTGCCGCGCGAACGGGAATACCGCgg                             >  2:94236/1‑150 (MQ=21)                                                                                                                      cacaTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGc                            <  2:1115208/150‑1 (MQ=255)                                                                                                                       acaTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCa                           >  2:1295372/1‑150 (MQ=255)                                                                                                                            gCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGAc                      <  1:640357/150‑1 (MQ=255)                                                                                                                                aGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCaa                  >  1:781170/1‑150 (MQ=255)                                                                                                                                     gTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAgcgc             >  1:125138/1‑150 (MQ=255)                                                                                                                                      ttAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCt            >  2:1845182/1‑150 (MQ=255)                                                                                                                                      ttAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCt            >  1:629875/1‑150 (MQ=255)                                                                                                                                          agCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCgg                                        >  1:1382717/1‑118 (MQ=255)                                                                                                                                          agCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCgg                                        <  2:1382717/118‑1 (MQ=255)                                                                                                                                            ccccaccaTGTCAAGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGa                       >  2:430798/3‑133 (MQ=255)                                                                                                                                            ccccaccaTGTCAAGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGa                       <  1:430798/131‑1 (MQ=255)                                                                                                                                            caccacCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCAtt      <  1:1926801/150‑1 (MQ=255)                                                                                                                                              ccaccaTGTCAAGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGTCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCtt           <  1:2222065/143‑1 (MQ=255)                                                                                                                                              ccaccaTGTCAAGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCtt           >  2:2222065/1‑143 (MQ=255)                                                                                                                                                accTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGaa  <  2:781170/150‑1 (MQ=255)                                                                                                                                                  cTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGGATACCGCGGCATGGACGCAAAGCGCTTTCAt       >  2:1870975/1‑143 (MQ=255)                                                                                                                                                  cTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGGATACCGCGGCATGGACGCAAAGCGCTTTCAt       <  1:1870975/143‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                TTTACGGGGCCCCCCTCAGTCAGCCTCTCTATAAAGAGTTTAAGATTCAGTAGGTTAGCGCGAAAAAAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAA  >  NC_002947/2796420‑2796712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑