Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,253,096 C→G 6.7% R130P (CGC→CCC)  PP_2851 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,253,0960CG 6.7% 321.5 / 9.7 99R130P (CGC→CCC) PP_2851hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (47/45);  new base G (3/4);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCACTCAAGCCGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTCCAGCCTTCGGCGAA  >  NC_002947/3252951‑3253244
                                                                                                                                                  |                                                                                                                                                    
aGCACTCAAGCCGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTg                                                                                                                                                  >  1:1823807/1‑150 (MQ=255)
 gCACTCAAGCCGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTgg                                                                                                                                                 <  2:1180531/150‑1 (MQ=255)
     tCAAGCCGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGaa                                                                                                                                             >  2:2009815/1‑150 (MQ=255)
          ccGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGcc                                                                                                                                        <  1:1458208/150‑1 (MQ=255)
           cGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAGTGCCt                                                                                                                                       >  1:992019/1‑150 (MQ=255)
                  cTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTc                                                                                                                                <  1:1216073/150‑1 (MQ=255)
                  cTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTc                                                                                                                                >  1:2139111/1‑150 (MQ=255)
                   tCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCg                                                                                                                               >  2:637219/1‑150 (MQ=255)
                    cGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCgg                                                                                                                              <  2:1128621/150‑1 (MQ=255)
                      cTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCa                                                                                                                            <  2:2139111/150‑1 (MQ=255)
                      cTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCa                                                                                                                            <  2:1140166/150‑1 (MQ=255)
                       tttCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCAt                                                                                                                           <  2:1971318/150‑1 (MQ=255)
                           aTTGCCCTCATACGGCTCATAGTGGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGAAGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACgg                                                                                                                       <  2:374848/150‑1 (MQ=255)
                            ttGACCTCATACGGCGCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGgc                                                                                                                      >  2:460478/1‑150 (MQ=255)
                              gCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGgcgc                                                                                                                    >  2:1391253/1‑150 (MQ=255)
                              gCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGgcgc                                                                                                                    <  2:848009/150‑1 (MQ=255)
                               cccTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCTGCATACGGCGcc                                                                                                                   >  2:1591466/1‑150 (MQ=255)
                                          cTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGcc                                                                                                                   <  2:923209/139‑1 (MQ=255)
                                          cTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGcc                                                                                                                   >  1:923209/1‑139 (MQ=255)
                                          cTCATAGTTGTACTGACGCTGGCATTCGCCGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTTCCATGCGACTAATACCACGGGAACTGCCAGCCCATGCGCTTGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGacccaca                                                                                                        <  1:2089108/150‑6 (MQ=255)
                                           tCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGcc                                                                                                       <  1:637219/150‑1 (MQ=255)
                                             aTAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCGAAGTCTTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCAGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGccac                                                                                                     >  1:259664/1‑150 (MQ=255)
                                             aTAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCGCATGGGCTgg                                                                                                                                                 >  1:1922757/1‑106 (MQ=255)
                                             aTAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCGCATGGGCTgg                                                                                                                                                 <  2:1922757/106‑1 (MQ=255)
                                                gTTGTACTGCCGCTTGCCTTCGCTGCCGACACTGACGCCGAAGTCTTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCAGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAg                                                                                                  <  2:259664/150‑1 (MQ=255)
                                                gTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACg                                                                                                                        <  1:1295006/128‑1 (MQ=255)
                                                gTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACg                                                                                                                        >  2:1295006/1‑128 (MQ=255)
                                                    tACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGgcgc                                                                                                                    <  1:1764414/128‑1 (MQ=255)
                                                    tACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGgcgc                                                                                                                    >  2:1764414/1‑128 (MQ=255)
                                                       tGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGAcaca                                                                                           >  2:196415/1‑150 (MQ=255)
                                                         ccGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATc                                                                                         >  1:468835/1‑150 (MQ=255)
                                                           gCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGACTACCACGGGAACTGCCAGCCCATGCGCGGGCTAAATGCCTGGAATTCGTCATACGACACCCGTGTCACCGCCACCAGCGACACATAgt                                                                                       >  2:2239499/1‑150 (MQ=255)
                                                           gCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCgt                                                                                       <  2:1823807/150‑1 (MQ=255)
                                                             tGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCCT‑TGAAGCCGCTGAAATGCGACCGATCCCAAGGGAAATGCCAGCCTATCGTTTTGGGATTTGCTGGGTTTGCTGCTGACGTCGCCCTTGACACCGCCACCAGCGACACATCgtgg                                                                                     <  2:263712/150‑1 (MQ=255)
                                                             cggCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑GCGTGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCgtgg                                                                                     <  1:1289045/149‑1 (MQ=255)
                                                                ccTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGtggtgg                                                                                  >  2:481681/1‑150 (MQ=255)
                                                                 cTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCGCATGGGCTGGcag                                                                                                                                              <  2:713616/89‑3 (MQ=255)
                                                                 cTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCGCATGGGCTGGcag                                                                                                                                              <  2:713956/89‑3 (MQ=255)
                                                                 cTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCGCATGGGCTGGcag                                                                                                                                              >  1:713956/1‑87 (MQ=255)
                                                                 cTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCGCATGGGCTGGcag                                                                                                                                              >  1:713616/1‑87 (MQ=255)
                                                                 cTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTggg                                                                                 >  1:1322079/1‑150 (MQ=255)
                                                                  ttCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCTCCAGCGACACATCGTGGTGGGc                                                                                >  1:1504024/1‑150 (MQ=255)
                                                                     gCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAGTGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAg                                                                             <  2:992019/150‑1 (MQ=255)
                                                                     gCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAg                                                                             <  1:1391253/150‑1 (MQ=255)
                                                                       tGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGt                                                                           <  1:2009815/150‑1 (MQ=255)
                                                                        gCCGACACTGACGCCAAAGTCCACGTGTGATCT‑GCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTg                                                                          <  1:1755260/150‑1 (MQ=255)
                                                                          cGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCa                                                                        >  2:1174051/1‑150 (MQ=255)
                                                                          cGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCa                                                                        >  2:2185566/1‑150 (MQ=255)
                                                                          cGACACTGACGCCAAAGTCCTCGT‑TGAAGGCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACGGCCACCAGCGGCACATCGTGGTGGGCCAGGTGCa                                                                        >  1:1134151/1‑150 (MQ=255)
                                                                           gACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTgg                                                                                                                                                 >  1:1982234/1‑76 (MQ=255)
                                                                           gACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTgg                                                                                                                                                 <  2:1982234/76‑1 (MQ=255)
                                                                           gACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAg                                                                       >  1:896240/1‑150 (MQ=255)
                                                                            acacTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGa                                                                      <  1:924174/150‑1 (MQ=255)
                                                                              acTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGAtt                                                                    >  2:1398360/1‑150 (MQ=255)
                                                                               cTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTg                                                                   <  1:481681/150‑1 (MQ=255)
                                                                               cTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTg                                                                   <  2:1252584/150‑1 (MQ=255)
                                                                                tGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGATATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTgg                                                                  >  1:476457/1‑150 (MQ=255)
                                                                                tGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGc                                                                                                        <  1:1655510/112‑1 (MQ=255)
                                                                                tGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGc                                                                                                        >  2:1655510/1‑112 (MQ=255)
                                                                                 gACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCAGAGCTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGgc                                                                 <  1:1591466/150‑1 (MQ=255)
                                                                                     ccAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCg                                                             >  2:2065799/1‑150 (MQ=255)
                                                                                      cAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGt                                                            >  1:340896/1‑150 (MQ=255)
                                                                                      cAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGt                                                            >  2:1694360/1‑150 (MQ=255)
                                                                                       aaaGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCCGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTc                                                           >  1:1179519/1‑150 (MQ=255)
                                                                                          gTCCTCGT‑TGAAGTCGCTGCCATGTTACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATa                                                        <  2:540642/150‑1 (MQ=255)
                                                                                             cTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGccacca                                                                                                   <  2:2196254/104‑1 (MQ=255)
                                                                                             cTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGccacca                                                                                                   >  1:2196254/1‑104 (MQ=255)
                                                                                             cTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACAACGCAACCAGCGATTCATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTg                                                     >  1:1907867/1‑150 (MQ=255)
                                                                                              tCGT‑TTAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAAt                                                                                                                                           >  2:2145555/1‑63 (MQ=255)
                                                                                              tCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAAt                                                                                                                                           <  1:2145555/63‑1 (MQ=255)
                                                                                              tCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTgg                                                    >  1:2181893/1‑150 (MQ=255)
                                                                                                gT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGc                                                                         >  2:1957758/1‑127 (MQ=255)
                                                                                                gT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGc                                                                         <  1:1957758/127‑1 (MQ=255)
                                                                                                 t‑tGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCg                                                 >  2:1198848/1‑150 (MQ=255)
                                                                                                       gTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAg                                            >  2:858359/1‑150 (MQ=255)
                                                                                                       gTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAg                                            >  2:679521/1‑150 (MQ=255)
                                                                                                          gCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGaaa                                         <  1:1398360/150‑1 (MQ=255)
                                                                                                             gCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAaga                                      <  2:340896/150‑1 (MQ=255)
                                                                                                              ccATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGATATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAagag                                     <  2:476457/150‑1 (MQ=255)
                                                                                                              ccATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAagag                                     >  2:2313715/1‑150 (MQ=255)
                                                                                                               cATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATa                                                        <  1:544730/130‑1 (MQ=255)
                                                                                                               cATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATa                                                        >  2:544730/1‑130 (MQ=255)
                                                                                                                aTGCGACGAATACCACGGGAACTGCCAGCCAATGCGCTGGAGAAATGCCTGGAATTCGGCATATGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTCGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGagca                                   <  1:1041864/150‑1 (MQ=255)
                                                                                                                      cgaATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCggg                             <  1:460478/150‑1 (MQ=255)
                                                                                                                       gaATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCgggg                            >  1:1752811/1‑150 (MQ=255)
                                                                                                                             cACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGccc                      >  1:1421708/1‑150 (MQ=255)
                                                                                                                               cGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCa                          >  2:1239145/1‑144 (MQ=255)
                                                                                                                               cGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCa                          <  1:1239145/144‑1 (MQ=255)
                                                                                                                                 ggAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGc                  <  1:1198848/150‑1 (MQ=255)
                                                                                                                                   aaCTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGc                                               <  1:2313231/119‑1 (MQ=255)
                                                                                                                                   aaCTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGc                                               >  2:2313231/1‑119 (MQ=255)
                                                                                                                                   aaCTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTc                <  2:468835/150‑1 (MQ=255)
                                                                                                                                   aaCTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTc                >  2:1502804/1‑150 (MQ=255)
                                                                                                                                   aaCTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTc                <  1:885718/150‑1 (MQ=255)
                                                                                                                                       gCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGc                  <  1:1369626/144‑1 (MQ=255)
                                                                                                                                       gCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGc                  >  2:1369626/1‑144 (MQ=255)
                                                                                                                                        ccAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTCCAGcc           >  1:339541/1‑150 (MQ=255)
                                                                                                                                            cccATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTCCAGCCTTCg       <  1:2313715/150‑1 (MQ=255)
                                                                                                                                             ccATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTCCAGCCTTCgg      <  1:1174051/150‑1 (MQ=255)
                                                                                                                                                 gcgcTGGCGAAACGCCTGGAATTCGGCATACGGCGCCCGTGACACCGAAACCAGCGACACATCGTGGTGGGCGAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTCCAGCCTTCGGCGaa  <  2:1134151/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGCACTCAAGCCGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGT‑TGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTCCAGCCTTCGGCGAA  >  NC_002947/3252951‑3253244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑