Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,368,464 G→A 5.8% R576H (CGC→CAC)  PP_2964 → transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,368,4640GA 5.8% 249.8 / 2.7 85R576H (CGC→CAC) PP_2964transposase
Reads supporting (aligned to +/- strand):  ref base G (41/39);  new base A (2/3);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

GGAGGAAACCGCGCAACAACTGGACGAGAAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAGCGCTACCTGATGACGATCTTCGCCA  >  NC_002947/3368315‑3368604
                                                                                                                                                     |                                                                                                                                             
ggaggaAACCGCGCAACAACTGGACGAGAAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAgcg                                                                                                                                               >  2:1717991/1‑150 (MQ=255)
ggaggaAACCGCGCAACAACTGGACGAGAAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAgcg                                                                                                                                               >  2:444761/1‑150 (MQ=255)
      aaCCGCGCAACAACTGGACGAGAAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGcc                                                                                                                                         <  1:190159/150‑1 (MQ=255)
            gcAACAACTGGACGAGAAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAg                                                                                                                                  >  1:1435407/1‑150 (MQ=255)
                      gACGAGAAATTCCCATCATGCCGAGGGGACGTATCGATTCACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCACATGCC‑TGGCCGGCATGCGCtg                                                                                                                        <  1:1191516/150‑2 (MQ=255)
                      gACGAGAAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGGGAACCGGTTTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGTGCATCTCGCTGCAGACGGCGCTTATGCTGCGCAGGCC‑GGCCAGGCATGTGCTc                                                                                                                        <  2:597516/150‑1 (MQ=255)
                      gACGAGAAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTc                                                                                                                        >  2:1827998/1‑150 (MQ=255)
                        cGAGAAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGa                                                                                                                      <  2:1435407/150‑1 (MQ=255)
                         gagaAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGGGGAGCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGat                                                                                                                     >  1:2287298/1‑150 (MQ=255)
                         gagaAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGat                                                                                                                     >  1:535010/1‑150 (MQ=255)
                         gagaAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGat                                                                                                                     >  1:1827943/1‑150 (MQ=255)
                         gagaAATGCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCAGTTGCTCGat                                                                                                                     >  2:315006/1‑150 (MQ=255)
                           gaAATTCCCATCATGCCGAGGGGCCGTAACGACTAACGAAGTAAGAGAACGGGTGTCGCGCGGAGGAGCGGCGGGGGGCATCCCCCCTTCGGCCATCTTGCTGCAGACGGCGCTGATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGaaac                                                                                                                   >  2:624691/1‑147 (MQ=255)
                           gaAATTCCCATCATGCCGAGGGGACGTATCGGTTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGAGCTCTGCGCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGatat                                                                                                                   >  1:2155606/1‑150 (MQ=255)
                             aaTTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGTCGCTGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCa                                                                                                                 <  1:631455/150‑1 (MQ=255)
                               ttCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATg                                                                                                               <  2:2234795/150‑1 (MQ=255)
                                tCCCCTCCTGCCGTGGGGTCGTCCCGATTAACGAGCCTGGAGAACCGGCGTTGCGCCGAGTAGCGGCGCGGGACACCCCCCCTTCGGCGATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATgg                                                                                                              <  1:688623/150‑1 (MQ=255)
                                tCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATgg                                                                                                              <  1:1886154/150‑1 (MQ=255)
                                  ccATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGcc                                                                                                                                    <  2:1866049/126‑1 (MQ=255)
                                  ccATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGcc                                                                                                                                    >  1:1866049/1‑126 (MQ=255)
                                  acatcatGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCt                                                                                                            <  2:869447/149‑1 (MQ=255)
                                   catcatGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCAtg                                                                                                                             <  1:1399752/132‑1 (MQ=255)
                                   catcatGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCAtg                                                                                                                             >  2:1399752/1‑132 (MQ=255)
                                   catcatGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCa                                                                                                                 >  2:1521236/1‑144 (MQ=255)
                                   catcatGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCa                                                                                                                 <  1:1521236/144‑1 (MQ=255)
                                    atcatGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTaa                                                                                                          >  2:9874/1‑150 (MQ=255)
                                       atGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACAt                                                                                                       <  2:1165792/150‑1 (MQ=255)
                                        tGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATc                                                                                                      <  2:1753048/150‑1 (MQ=255)
                                          ccGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGa                                                                                                    <  1:2133315/150‑1 (MQ=255)
                                              ggggACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCAt                                                                                                <  1:99455/150‑1 (MQ=255)
                                              ggggACGTATCGATTAACCAAGATGGAGAAACGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCAt                                                                                                <  2:2287298/150‑1 (MQ=255)
                                               gggACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCAtt                                                                                               <  1:1717991/150‑1 (MQ=255)
                                                 gACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTgg                                                                                             >  2:1006237/1‑150 (MQ=255)
                                                  aCGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCACCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGa                                                                                            <  2:971642/150‑1 (MQ=255)
                                                      aTCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTct                                                                                        <  1:1835419/150‑2 (MQ=255)
                                                      aTCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCa                                                                                        >  2:887095/1‑150 (MQ=255)
                                                       tCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCg                                                                                                     <  1:250757/136‑1 (MQ=255)
                                                       tCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCg                                                                                                     >  2:250757/1‑136 (MQ=255)
                                                       tCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGAGGCGCGCCACATTCCCCCCACCCCCACGTGCATCCCCCCGACCACGACGCGCTGCATGCC‑TGTCGGGCATGGTGTCGATATCATGGCTAACATCGAGCATTGGATTCAg                                                                                       <  2:2017130/150‑1 (MQ=255)
                                                        cGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGt                                                                                      <  1:2078488/150‑1 (MQ=255)
                                                              aCGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCAcgc                                                                                <  1:1827998/150‑1 (MQ=255)
                                                               cGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTg                                                                                              <  1:667243/135‑1 (MQ=255)
                                                               cGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTg                                                                                              >  2:667243/1‑135 (MQ=255)
                                                                    cTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCAtt                                                                          >  2:1418177/1‑150 (MQ=255)
                                                                     tGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCa                                                                                        >  2:330055/1‑135 (MQ=255)
                                                                     tGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCa                                                                                        <  1:330055/135‑1 (MQ=255)
                                                                      ggAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATgg                                                                                                              >  1:2192410/1‑112 (MQ=255)
                                                                      ggAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATgg                                                                                                              <  2:2192410/112‑1 (MQ=255)
                                                                      ggAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTc                                                                        <  1:1006237/150‑1 (MQ=255)
                                                                      ggAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTc                                                                        >  2:1338210/1‑150 (MQ=255)
                                                                       gagaACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGTC‑GGCCAGGCATGTGCTTGATATCATGGCTAACATCGGGCCTTGGATTCAGTTCACGCGGCATTTCg                                                                       >  1:1249699/1‑150 (MQ=255)
                                                                         gaACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCggg                                                                     <  2:535010/150‑1 (MQ=255)
                                                                          aaCCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAg                                                                                                   >  2:1835705/1‑119 (MQ=255)
                                                                          aaCCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAg                                                                                                   <  1:1835705/119‑1 (MQ=255)
                                                                             cGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGata                                                                                                                    <  2:859062/99‑1 (MQ=255)
                                                                             cGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGata                                                                                                                    >  1:859062/1‑99 (MQ=255)
                                                                              ggTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGc                                                                    <  1:199474/146‑1 (MQ=255)
                                                                              ggTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGc                                                                    >  2:199474/1‑146 (MQ=255)
                                                                              ggTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCACATGCCTGGCC‑GGCATGCGCtgc                                                                                                                       <  1:1619634/95‑3 (MQ=37)
                                                                              ggTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCACATGCCTGGCC‑GGCATGCGCtgc                                                                                                                       >  2:1619634/1‑93 (MQ=37)
                                                                                     cgcCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGt                                                         >  1:131512/1‑150 (MQ=255)
                                                                                      gcCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTa                                                        >  1:480061/1‑150 (MQ=255)
                                                                                                 gcgcGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAg                                             >  1:218916/1‑150 (MQ=255)
                                                                                                           tCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGc                                   >  2:750194/1‑150 (MQ=255)
                                                                                                           tCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGc                                   >  1:1225042/1‑150 (MQ=255)
                                                                                                            ccccccTTCCGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGcc                                  <  1:957787/150‑1 (MQ=255)
                                                                                                                 cTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCg                                                           >  2:1913599/1‑120 (MQ=255)
                                                                                                                 cTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCg                                                           <  1:1913599/120‑1 (MQ=255)
                                                                                                                  gtCGGCCAGCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGa                            <  1:624691/149‑1 (MQ=255)
                                                                                                                   tCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAg                           <  2:154667/150‑1 (MQ=255)
                                                                                                                     ggCCATCTCGCTGCAGACGGCGCTCATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAgcg                         >  1:1235144/1‑150 (MQ=255)
                                                                                                                      gCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAgcgc                        >  2:2150835/1‑150 (MQ=255)
                                                                                                                        cATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAGCGCTa                      >  1:804107/1‑150 (MQ=255)
                                                                                                                         aTCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAGCGCTAc                     <  1:1418177/150‑1 (MQ=255)
                                                                                                                          tctcGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTTGGTGCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAgatcggaa                    >  1:2261498/1‑143 (MQ=255)
                                                                                                                            tcGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAGCGCTACCtg                  <  2:1225042/150‑1 (MQ=255)
                                                                                                                            tcGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAGCGCTACCtg                  >  1:112557/1‑150 (MQ=255)
                                                                                                                               cTGCAGACTGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAGCGCTACCtgatg               <  1:444761/150‑1 (MQ=255)
                                                                                                                                  cAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAGCGCTACCTGATGACg            >  2:256575/1‑150 (MQ=255)
                                                                                                                                          gcTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGGTAAAAGAGCCTGCCGAGCGCTACCTGATGACGATCTTCGc    >  1:1288798/1‑150 (MQ=255)
                                                                                                                                          gcTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAGCGCTACCTGATGACGATCTTCGc    >  2:1695857/1‑150 (MQ=255)
                                                                                                                                           cTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAGCGCTACCTGATGACGATCTTCGcc   >  2:1206283/1‑150 (MQ=255)
                                                                                                                                            ttATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCt                                 <  1:1762367/119‑1 (MQ=255)
                                                                                                                                            ttATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCt                                 >  2:1762367/1‑119 (MQ=255)
                                                                                                                                            ttATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAGCGCTACCTGATGACGATCTTCGCCa  >  1:784036/1‑150 (MQ=255)
                                                                                                                                               tcgaGCACATGCC‑TGGCCGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCAcgcg                                                                               <  1:1874822/67‑1 (MQ=25)
                                                                                                                                               tcgaGCACATGCC‑TGGCCGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCAcgcg                                                                               >  2:1874822/4‑70 (MQ=25)
                                                                                                                                                     |                                                                                                                                             
GGAGGAAACCGCGCAACAACTGGACGAGAAATTCCCATCATGCCGAGGGGACGTATCGATTAACGAAGCTGGAGAACCGGTGTTGCGCCGAGTAGCGGCGCGGGACATCCCCCCTTCGGCCATCTCGCTGCAGACGGCGCTTATGCAGCGCATGCC‑GGCCAGGCATGTGCTCGATATCATGGCTAACATCGAGCATTGGATTCAGTTCACGCGGCATTTCGGGCCCATGTCCGGTAACGAGCCGAAGCTCAAAGAGCCTGCCGAGCGCTACCTGATGACGATCTTCGCCA  >  NC_002947/3368315‑3368604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑