BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,688,223	G→T	7.3%	L99L (CTG→CTT)	PP_3253 →	carboxylate‑amine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,688,223	0	G	T	7.3%	300.4 / 8.5	96	L99L (CTG→CTT)	PP_3253	carboxylate‑amine ligase
Reads supporting (aligned to +/- strand): ref base G (47/42); new base T (2/5); total (49/47)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 103 total aligned reads displayed.

CAGATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCT  >  NC_002947/3688077‑3688369

cAGATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGctgctg                                                                                                                                                  >  1:1006251/1‑150 (MQ=255)

  gATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCtgctgc                                                                                                                                                 >  2:287980/1‑149 (MQ=255)

  gATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCtgctgc                                                                                                                                                 <  1:287980/149‑1 (MQ=255)

  gATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTACGCGTGGCATTGGCCGAAGAGGGGATGGGGCCAGATGCAGCGGCAGGCCAACCCTGGGCCTCGTGGCtgctgct                                                                                                                                                <  2:366151/150‑2 (MQ=255)

     ctaGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGaa                                                                                                                                             <  1:413457/148‑1 (MQ=255)

        gCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGcc                                                                                                                                          <  1:831349/150‑1 (MQ=255)

         cTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGcc‑c                                                                                                                                        >  2:2034998/1‑150 (MQ=255)

                  ccGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCGGcgcag                                                                                                                               <  1:47020/150‑2 (MQ=38)

                   cGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCGGcgcagg                                                                                                                              <  2:423209/150‑3 (MQ=38)

                     gtgtTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCGGAACAGGGGGTGGTGCTGTATGGGCCGGCAAGCCACGTCGGCGCCGCGTTGCTGCTGCAGAAGCC‑CGCGGCACAAggg                                                                                                                            <  2:2017651/150‑1 (MQ=255)

                     gtgtTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCACCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAggg                                                                                                                            <  2:1752566/150‑1 (MQ=255)

                        ttCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCAc                                                                                                                         >  2:1923652/1‑150 (MQ=255)

                        ttCACCAACCTGGATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCAc                                                                                                                         >  2:1399895/1‑150 (MQ=255)

                         tCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCTTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCGGCGCAggggtggc                                                                                                                        <  1:1989127/150‑5 (MQ=37)

                          cACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACta                                                                                                                       >  2:1449502/1‑150 (MQ=255)

                          cACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACta                                                                                                                       >  1:1156385/1‑150 (MQ=255)

                           aCCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACtat                                                                                                                      >  2:1996253/1‑150 (MQ=255)

                            ccAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACtata                                                                                                                     >  2:1934906/1‑150 (MQ=255)

                              aaCCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGTGCACTATAag                                                                                                                   <  1:510576/150‑1 (MQ=255)

                              aaCCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAag                                                                                                                   <  2:2154344/150‑1 (MQ=255)

                                ccTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAagca                                                                                                                 >  2:1952886/1‑150 (MQ=255)

                                   gTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCt                                                                                                              >  1:1984977/1‑150 (MQ=255)

                                   gTATGAAGCACGTGACTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCt                                                                                                              <  2:1622853/150‑1 (MQ=255)

                                      tGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGtt                                                                                                           >  1:508089/1‑150 (MQ=255)

                                      tGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCATGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGtt                                                                                                           >  1:581037/1‑150 (MQ=255)

                                          gCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                       <  1:753953/150‑1 (MQ=255)

                                            aCGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgacga                                                                                                     >  2:2010084/1‑150 (MQ=255)

                                             cGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCgacgac                                                                                                    >  2:1010794/1‑150 (MQ=255)

                                              gTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACt                                                                                                   >  2:1126495/1‑150 (MQ=255)

                                              gTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACt                                                                                                   >  1:2159235/1‑150 (MQ=255)

                                                  gTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCg                                                                                               >  1:77886/1‑150 (MQ=255)

                                                       tcCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCAtg                                                                                          >  2:938882/1‑150 (MQ=255)

                                                         cAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑cg                                                                                                                                       <  1:1981819/103‑1 (MQ=255)

                                                         cAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑cg                                                                                                                                       >  2:1981819/1‑103 (MQ=255)

                                                          aGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGAGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTgg                                                                                       >  1:1705491/1‑150 (MQ=255)

                                                           gcgcAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGcc                                                                                             >  1:1015541/1‑143 (MQ=255)

                                                           gcgcAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGcc                                                                                             <  2:1015541/143‑1 (MQ=255)

                                                              ccaCCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGACGCGTGGCTGCTGCAGAAGCC‑CCCGGCACAAGGGCACTATACGCTGCTGTTCGACGACTACCCCCATGTGACAAg                                                                                   <  2:689744/148‑1 (MQ=255)

                                                              cAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAg                                                                                   <  2:1006251/150‑1 (MQ=255)

                                                                aCCGTCAGCGGCTGCGCGTGGCATTGGACGAAGAGGGGATGGGGCCATACGCTGCCGAAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGc                                                                                 <  2:42490/150‑1 (MQ=255)

                                                                 ccGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACAACTGCGCCGCTGGGTTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCg                                                                                <  1:2034998/150‑1 (MQ=255)

                                                                  cGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCgg                                                                                                                                    >  1:1150516/1‑97 (MQ=25)

                                                                  cGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGTGCCGCG‑GGCTTCTGCAGCAGCCACGCgg                                                                                                                                    <  2:1150516/97‑1 (MQ=25)

                                                                   gTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTa                                                                              <  1:1126495/150‑1 (MQ=255)

                                                                     cAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGc                                                                            <  1:1996253/150‑1 (MQ=255)

                                                                     cAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGc                                                                            <  1:1952886/150‑1 (MQ=255)

                                                                      aGCGGCTGCGCGTGGCATTGGCCGAAAAGGGGAGGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGcc                                                                           <  2:1705491/150‑1 (MQ=255)

                                                                          gCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCt                                                                       <  1:1010794/150‑1 (MQ=255)

                                                                              cgcgTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTTGCAAGGCGTAGCCTGCTCAAt                                                                   <  1:1449502/150‑1 (MQ=255)

                                                                                cgTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCt                                                                          >  2:607371/1‑141 (MQ=255)

                                                                                cgTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCt                                                                          <  1:607371/141‑1 (MQ=255)

                                                                                 gTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGc                                                                <  2:2159235/150‑1 (MQ=255)

                                                                                      aTTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGcc‑c                                                                                                                                        <  2:1984592/73‑1 (MQ=255)

                                                                                      aTTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGcc‑c                                                                                                                                        >  1:1984592/1‑73 (MQ=255)

                                                                                        tGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCAtg                                                         <  1:854556/150‑1 (MQ=255)

                                                                                         ggCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCAtgt                                                        >  1:447311/1‑150 (MQ=255)

                                                                                         ggCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGAAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCAtgt                                                        >  2:1484353/1‑150 (MQ=255)

                                                                                             gAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCG‑GGCTTCTGCAGCAGCCACGCgg                                                                                                                                    >  1:109630/1‑70 (MQ=21)

                                                                                             gAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCG‑GGCTTCTGCAGCAGCCACGCgg                                                                                                                                    <  2:109630/70‑1 (MQ=21)

                                                                                             gAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCACTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCAc                                                    <  1:611346/150‑1 (MQ=255)

                                                                                                 agGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTg                                                <  1:1484353/150‑1 (MQ=255)

                                                                                                  ggggATGGGGCCATACGCTGCGGCAAGCCACACCTGCGCCGCGTGGCTGCTGAAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTGAATGGCCTGCATGTGCACGTTgg                                               >  1:974825/1‑150 (MQ=255)

                                                                                                   gggATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGc                                              <  2:1984977/150‑1 (MQ=255)

                                                                                                   gggATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGc                                              <  2:400894/150‑1 (MQ=255)

                                                                                                     gATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGt                                            >  2:1049615/1‑150 (MQ=255)

                                                                                                     gATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTCGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGt                                            >  2:893861/1‑150 (MQ=255)

                                                                                                        ggggCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgcc                                         >  1:1530646/1‑150 (MQ=255)

                                                                                                          ggCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTCATCTTCTCAATGGCCTGCATGTGCACGTTGGCGTgccgc                                       >  1:122738/1‑150 (MQ=255)

                                                                                                          ggCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgccgc                                       >  1:1234609/1‑150 (MQ=255)

                                                                                                          ggCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgccgc                                       >  2:1263710/1‑150 (MQ=255)

                                                                                                           gCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAATGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgccgcc                                      >  1:142386/1‑150 (MQ=255)

                                                                                                           gCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgccgcc                                      >  1:1810435/1‑150 (MQ=255)

                                                                                                             cATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                       >  1:703697/1‑83 (MQ=255)

                                                                                                             cATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                       <  2:703697/83‑1 (MQ=255)

                                                                                                              aTACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                       >  1:2147009/1‑82 (MQ=255)

                                                                                                              aTACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTcgac                                                                                                       <  2:2147009/82‑1 (MQ=255)

                                                                                                                aCGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCtt                                 <  1:1584381/150‑1 (MQ=255)

                                                                                                                aCGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCtt                                 <  1:1733877/150‑1 (MQ=255)

                                                                                                                aCGCTGCGGCAAGCCACCCCTGCGCCGCATGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCtt                                 <  2:581037/150‑1 (MQ=255)

                                                                                                                  gCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCTACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTtgt                               <  2:189505/150‑1 (MQ=255)

                                                                                                                  gCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTtgt                               <  1:877601/150‑1 (MQ=255)

                                                                                                                   cTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTtgtg                              >  1:2171137/1‑150 (MQ=255)

                                                                                                                    tGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGa                             >  1:2199779/1‑150 (MQ=255)

                                                                                                                      cGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTCCCGCCATGTGGCAAGGCGTCGAGGGCGCAGTAGTCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGAcc                           <  1:988956/150‑1 (MQ=255)

                                                                                                                        gCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGc                         >  2:475389/1‑150 (MQ=255)

                                                                                                                           aGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATg                      <  1:1934906/150‑1 (MQ=255)

                                                                                                                           aGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATg                      >  2:455662/1‑150 (MQ=255)

                                                                                                                            gCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGc                     <  1:2105379/150‑1 (MQ=255)

                                                                                                                             ccACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCa                    >  1:2101734/1‑150 (MQ=255)

                                                                                                                             ccACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCa                    >  1:1863401/1‑150 (MQ=255)

                                                                                                                               aCCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAAc                  <  1:475389/150‑1 (MQ=255)

                                                                                                                                ccccTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACt                 >  2:2065726/1‑150 (MQ=255)

                                                                                                                                  ccTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGa               <  1:1263710/150‑1 (MQ=255)

                                                                                                                                  ccTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGGAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGa               >  1:1313410/1‑150 (MQ=255)

                                                                                                                                   cTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGAt              >  2:568658/1‑150 (MQ=255)

                                                                                                                                       gccgcGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAAc          >  1:907147/1‑150 (MQ=255)

                                                                                                                                           cgTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGcc      <  2:86840/150‑1 (MQ=255)

                                                                                                                                               gctgctGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCt  <  1:872623/150‑1 (MQ=255)

                                                                                                                                                 tgctgcAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGAcc                           <  2:544064/123‑1 (MQ=255)

                                                                                                                                                 tgctgcAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGAcc                           >  1:544064/1‑123 (MQ=255)

CAGATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCC‑CGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAACCGCCTTCT  >  NC_002947/3688077‑3688369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`