Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,793,940 A→T 8.1% I209N (ATT→AAT)  PP_3355 ← beta‑ketothiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,793,9400AT 8.1% 259.2 / 13.4 87I209N (ATT→AAT) PP_3355beta‑ketothiolase
Reads supporting (aligned to +/- strand):  ref base A (39/40);  new base T (3/4);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

GCGCCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCATA  >  NC_002947/3793799‑3794088
                                                                                                                                             |                                                                                                                                                    
gcgcCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCATTGCATTGCCGCGt                                                                                                                                              <  1:1993995/150‑1 (MQ=255)
gcgcCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGt                                                                                                                                              <  1:676327/150‑1 (MQ=255)
gcgcCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGt                                                                                                                                              <  1:695925/150‑1 (MQ=255)
 cgcCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGTCACGCGGCAATGCAATGCCGCGtg                                                                                                                                             <  2:109883/150‑1 (MQ=255)
 cgcCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCATTGCATTGCCGCGtg                                                                                                                                             <  2:521715/150‑1 (MQ=255)
 cgcCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGtg                                                                                                                                             <  2:2187410/150‑1 (MQ=255)
    cATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGtggct                                                                                                                                          >  1:655563/1‑150 (MQ=255)
          ccaccGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGc                                                                                                                                    <  1:1506096/150‑1 (MQ=255)
           caccGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGcc                                                                                                                                   >  1:703251/1‑150 (MQ=255)
            accGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCGATGCCGCGtg                                                                                                                                             >  1:916163/1‑139 (MQ=255)
            accGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCGATGCCGCGtg                                                                                                                                             <  2:916163/139‑1 (MQ=255)
             ccGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCtt                                                                                                                                 >  1:676154/1‑150 (MQ=255)
               gcgcAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCATTGCATTGCCGCGtggcgt                                                                                                                                         >  1:222535/1‑138 (MQ=255)
               gcgcAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCATTGCATTGCCGCGtggcgt                                                                                                                                         <  2:222535/140‑3 (MQ=255)
               gcgcAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCt                                                                                                                               <  2:655563/150‑1 (MQ=255)
               gcgcAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCt                                                                                                                               >  1:999091/1‑150 (MQ=255)
                cgcAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTa                                                                                                                              <  2:703251/150‑1 (MQ=255)
                cgcAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTa                                                                                                                              >  2:1956625/1‑150 (MQ=255)
                   aaCTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCt                                                                                                                           >  2:987000/1‑150 (MQ=255)
                        ttGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTc                                                                                                                                <  2:1927271/140‑1 (MQ=255)
                        ttGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTc                                                                                                                                >  1:1927271/1‑140 (MQ=255)
                        ttGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGt                                                                                                                      <  2:2000821/150‑1 (MQ=255)
                        ttGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGt                                                                                                                      >  2:797981/1‑150 (MQ=255)
                        ttGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGt                                                                                                                      <  2:999091/150‑1 (MQ=255)
                          gCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGc                                                                                                                    <  2:2091847/150‑1 (MQ=255)
                          gCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGc                                                                                                                    >  1:831825/1‑150 (MQ=255)
                              gCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTgc                                                                                                                <  1:2294702/150‑1 (MQ=255)
                               ccGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTgcg                                                                                                               >  1:2204632/1‑150 (MQ=255)
                                 gTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGcgcg                                                                                                             >  2:535162/1‑150 (MQ=255)
                                  tCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTAc                                                                                                                             <  1:1965097/133‑1 (MQ=255)
                                  tCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTAc                                                                                                                             >  2:1965097/1‑133 (MQ=255)
                                    tGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGtggc                                                                                                                                           <  2:1747107/117‑1 (MQ=255)
                                    tGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGtggc                                                                                                                                           >  1:1747107/1‑117 (MQ=255)
                                         gCCACCGGCGTGCACCGGGCGCAGTCGGGCGAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTGCTCCCTCCAGTGCCGGCTCGGGGAGGCt                                                                                                     >  2:1969371/1‑150 (MQ=255)
                                           cACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGa                                                                                                   <  1:203498/150‑1 (MQ=255)
                                            aCCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCATGCCACGCGGCATTGCATTGCCGCGtggcgtga                                                                                                                                       <  1:2138024/113‑5 (MQ=38)
                                            aCCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCATGCCACGCGGCATTGCATTGCCGCGtggcgtga                                                                                                                                       >  2:2138024/1‑109 (MQ=38)
                                                gCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGTGCGGTGACGCTGACGAtt                                                                                              <  1:4468/150‑1 (MQ=255)
                                                gCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGAtt                                                                                              <  2:204072/150‑1 (MQ=255)
                                                     cACCGGGCGCAGTCGGGCCAGCGCAGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACTCGGCAATGCAATGCCGCGTGGCTGGCAGCCATCTACCTCCAGTGCCTGCGTGGTGACGCTGACGATTTCCtc                                                                                         <  2:183383/150‑1 (MQ=255)
                                                       ccGGGCGCAGTCGGTCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtcgt                                                                                       <  2:602333/150‑1 (MQ=255)
                                                        cgagcgcAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtcgtc                                                                                      >  2:2145243/4‑150 (MQ=255)
                                                        cGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtcgtc                                                                                      >  2:18752/1‑150 (MQ=255)
                                                            cgcAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAAc                                                                                  <  2:2291888/150‑1 (MQ=255)
                                                             gcAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAAcc                                                                                 >  2:1648562/1‑150 (MQ=255)
                                                                   gggCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCT                                                                           >  1:1526744‑M1/1‑150 (MQ=255)
                                                                    ggCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTG                                                                          >  1:1234138‑M1/1‑150 (MQ=255)
                                                                       cAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCAc                                                                       <  1:535162/150‑1 (MQ=255)
                                                                           gCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTgg                                                                   <  1:1603041/150‑1 (MQ=255)
                                                                                 gcgTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCtt                                                                                                                                 >  1:1398400/1‑82 (MQ=255)
                                                                                 gcgTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCtt                                                                                                                                 <  2:1398400/82‑1 (MQ=255)
                                                                                   gTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTtgcag                                                           <  2:998838‑M2/150‑6 (MQ=255)
                                                                                   gTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTt                                                               >  1:433142‑M2/1‑145 (MQ=255)
                                                                                   gTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTt                                                               <  2:433142‑M2/146‑2 (MQ=255)
                                                                                          ggCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCGCCTGGGCTTGCAGGGCCCGc                                                    <  1:2138049/150‑1 (MQ=255)
                                                                                             cGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGTCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCA                                                                        <  1:1733005‑M1/127‑1 (MQ=255)
                                                                                             cGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGTCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCA                                                                        >  2:1733005‑M1/1‑127 (MQ=255)
                                                                                              gtgggtggCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCAagcccaggtgcagggctggttcga                                                <  1:1969371‑M1/150‑25 (MQ=255)
                                                                                              gtgggtggCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGAt                                                <  2:1234138/150‑1 (MQ=255)
                                                                                               tgggtggCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATg                                               <  2:2204632/150‑1 (MQ=255)
                                                                                                 ggtggCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtcgt                                                                                       <  2:95993/108‑1 (MQ=255)
                                                                                                 ggtggCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCtcgt                                                                                       >  1:95993/1‑108 (MQ=255)
                                                                                                   tggCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTg                                           >  1:1233264/1‑150 (MQ=255)
                                                                                                       tGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCa                                       >  1:1189146/1‑150 (MQ=255)
                                                                                                         tCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAgg                                     <  2:1233264/150‑1 (MQ=255)
                                                                                                         tCCTGGCTGACCTCACTCACGCCACGCGCCAATGCACTGCCGAGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTCCACGGCCCGCTCATGGCTGCGCAgg                                     >  2:178933/1‑150 (MQ=255)
                                                                                                          ccTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCGCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAggg                                    >  1:384173/1‑150 (MQ=255)
                                                                                                           cTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGc                                   >  1:1784405/1‑150 (MQ=255)
                                                                                                            tGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGcc                                  <  1:1956625/150‑1 (MQ=255)
                                                                                                            tGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGcc                                  >  2:578387/1‑150 (MQ=255)
                                                                                                               cTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCCCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCAc                               >  1:1837173/1‑150 (MQ=255)
                                                                                                                   ccTCACTCACGCCACGCGGCAATGCAATGACGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACACTGACGATTTCCTCGACGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGTAGCGCCCACGCGt                           >  2:2234884/1‑150 (MQ=255)
                                                                                                                    ctcactcaCGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTgg                                                                   >  2:141037/1‑109 (MQ=255)
                                                                                                                    ctcactcaCGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTgg                                                                   <  1:141037/109‑1 (MQ=255)
                                                                                                                    ctcactcaCGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTc                          >  2:669865/1‑150 (MQ=255)
                                                                                                                    ctcactcaCGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTc                          <  2:1845660/150‑1 (MQ=255)
                                                                                                                     tcactcacGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCAGCTGGGCTTGCAGGGACCGCTGATGGCTGCGCGGGGcacaggggtgc                         >  2:1128296/1‑143 (MQ=255)
                                                                                                                         tcacGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCg                     >  1:1808992/1‑150 (MQ=255)
                                                                                                                            cgccacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGttt                  <  1:1281927/150‑1 (MQ=255)
                                                                                                                             gccacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTg                                                                                                                 >  2:1978368/1‑54 (MQ=255)
                                                                                                                             gccacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTg                                                                                                                 <  1:1978368/54‑1 (MQ=255)
                                                                                                                               cacgcGGCATTGCATTGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCAc               >  2:341349/1‑150 (MQ=255)
                                                                                                                               cacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAgg                                     >  2:215980/1‑128 (MQ=255)
                                                                                                                               cacgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAgg                                     <  1:215980/128‑1 (MQ=255)
                                                                                                                                acgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAAc                                                                                  <  2:1662567/82‑1 (MQ=255)
                                                                                                                                acgcGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAAc                                                                                  >  1:1662567/1‑82 (MQ=255)
                                                                                                                                    ggCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGcc          >  1:1824854/1‑150 (MQ=255)
                                                                                                                                     gcaatgcaatGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCa         <  1:2145243/150‑1 (MQ=255)
                                                                                                                                     gcaatgcaatGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCa         <  1:178933/150‑1 (MQ=255)
                                                                                                                                      cattgcattgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAg        <  1:341349/147‑1 (MQ=255)
                                                                                                                                      caatgcaatgCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCCCCGTTTCACGCGCCAg        >  2:1219129/1‑150 (MQ=255)
                                                                                                                                         tgcaatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGc                                                                 >  1:141466/1‑90 (MQ=255)
                                                                                                                                         tgcaatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGc                                                                 <  2:141466/90‑1 (MQ=255)
                                                                                                                                         tgcaatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCGCCGTTTCACGCGCCAGGcc     >  2:567420/1‑150 (MQ=255)
                                                                                                                                           caatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCAt   <  1:578387/150‑1 (MQ=255)
                                                                                                                                            aatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCAagcccaggtgcagggctggtt                                                   <  1:16905‑M1/101‑22 (MQ=255)
                                                                                                                                            aatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCAagcccaggtgcagggctggtt                                                   >  2:16905‑M1/1‑80 (MQ=255)
                                                                                                                                            aatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCAcgc                             <  1:1998957/123‑1 (MQ=255)
                                                                                                                                            aatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCAcgc                             >  2:1998957/1‑123 (MQ=255)
                                                                                                                                            aatgcCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCATa  >  1:133839/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GCGCCATCGACCACCGCGCAACTGTTGCCAGCCGTCTGCACGCCACCGGCGTGCACCGGGCGCAGTCGGGCCAGTGCGGCCGCGTCGGTAGGCCGTGGGTGGCTGTCCTGGCTGACCTCACTCACGCCACGCGGCAATGCAATGCCGCGTGGCTGGCAGCCTTCTACCTCCAGTGCCTGCGCGGTGACGCTGACGATTTCCTCGTCGAACCAGCCCTGCACCTGGGCTTGCAGGGCCCGCTGATGGCTGCGCAGGGCCCACGCGTCCACCGTTTCACGCGCCAGGCCATA  >  NC_002947/3793799‑3794088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑