BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,920,317	C→T	7.3%	R498Q (CGG→CAG)	acs ←	acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,920,317	0	C	T	7.3%	315.6 / 11.1	109	R498Q (CGG→CAG)	acs	acyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base C (52/49); new base T (5/3); total (57/52)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 110 total aligned reads displayed.

CCGGAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGTCCTTGCTGCGC  >  NC_002947/3920175‑3920466

ccGGACTTTATGCACCTGGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAACCCCGGGGCACCTTGAACTCCGAAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCa                                                                                                                                                <  1:754824/150‑1 (MQ=255)

 cGGAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAg                                                                                                                                               <  2:2181035/150‑1 (MQ=255)

 cGGAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAg                                                                                                                                               <  1:643054/150‑1 (MQ=255)

  ggAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCGGCTGCACCCggg                                                                                                                                              >  1:441617/1‑150 (MQ=255)

  ggAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCGGCTGCACCCggg                                                                                                                                              <  2:441617/150‑1 (MQ=255)

     aTTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCa                                                                                                                                           >  2:545420/1‑150 (MQ=255)

        tCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGa                                                                                                                                        >  1:1557674/1‑150 (MQ=255)

        tCTGCACCTTGCCCGTCACCGTCATCGGCAAAAGGTCGGTAAAACGGAAATACGTGGGCACGTTGAAGTGGGAAAACACAGATGGAAAACGACCTCGGAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGa                                                                                                                                        >  1:2280225/1‑150 (MQ=255)

         cTGCACCTTGCCCGGCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGAt                                                                                                                                       <  1:1925920/150‑1 (MQ=255)

            cACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGAtctc                                                                                                                                    >  2:606151/1‑150 (MQ=255)

             aCCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATctct                                                                                                                                   <  2:737989/150‑1 (MQ=255)

             aCCTTGCCCGACACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGTGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATctct                                                                                                                                   >  2:1842620/1‑150 (MQ=255)

               cTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTc                                                                                                                                 >  1:316534/1‑150 (MQ=255)

                ttGCCCGTCATCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCa                                                                                                                                >  2:462871/1‑150 (MQ=255)

                 tGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCAc                                                                                                                               <  2:1629471/150‑1 (MQ=255)

                  gCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCAcc                                                                                                                              >  1:1243174/1‑150 (MQ=255)

                          ccgtcaTCGGGAACTCGTCGACAAAACCGAAATACCGCTTCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCACGGGTGCAGCCGCACCCAGGCCCCGATCTCTTCACCGTACTtgc                                                                                                                      <  1:1072293/150‑1 (MQ=255)

                             tcaTCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTtgctgc                                                                                                                   >  1:1110042/1‑150 (MQ=255)

                               aTCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAg                                                                                                                 <  2:292223/150‑1 (MQ=255)

                                tCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAgg                                                                                                                <  2:2074805/150‑1 (MQ=255)

                                tCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAgg                                                                                                                <  2:2280225/150‑1 (MQ=255)

                                  gggAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGc                                                                                                              >  1:1543886/1‑150 (MQ=255)

                                  gggAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAAACCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGc                                                                                                              >  1:153893/1‑150 (MQ=255)

                                   ggAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGc                                                                                                                                             >  1:1948999/1‑118 (MQ=255)

                                   ggAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGc                                                                                                                                             <  2:1948999/118‑1 (MQ=255)

                                   ggAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCa                                                                                                             <  1:664982/150‑1 (MQ=255)

                                         tgtcgACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCaa                                                                                                       <  1:34387/149‑1 (MQ=255)

                                          gtcgACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAAt                                                                                                      >  2:635807/1‑150 (MQ=255)

                                          gtcgACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAAt                                                                                                      >  2:1573389/1‑150 (MQ=255)

                                             gACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCAc                                                                                                   <  1:1267828/150‑1 (MQ=255)

                                              aCAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCAc                                                                                                   >  2:1315256/1‑149 (MQ=255)

                                              aCAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCAc                                                                                                   <  1:1315256/149‑1 (MQ=255)

                                                aaaaCGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCGGCTGCAcccgg                                                                                                                                               <  1:1873128/103‑3 (MQ=255)

                                                aaaaCGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCGGCTGCAcccgg                                                                                                                                               >  2:1873128/1‑101 (MQ=255)

                                                   aCGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTGACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGcac                                                                                             >  2:1679370/1‑150 (MQ=255)

                                                           aCCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCa                                                                                     <  1:1842620/150‑1 (MQ=255)

                                                            ccGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCAc                                                                                    >  1:850544/1‑150 (MQ=255)

                                                                   aCCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCAc                                                                                                                                          <  2:654642/89‑1 (MQ=255)

                                                                   aCCTTGAAGTGCGCAATCCTTGCCCTAGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCAc                                                                                                                                          >  1:654642/1‑89 (MQ=255)

                                                                     cTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCg                                                                                         <  1:1086000/136‑1 (MQ=255)

                                                                     cTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCgggtg                                                                           <  2:1543886/150‑1 (MQ=255)

                                                                     cTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCgggtg                                                                           <  1:1821432/150‑1 (MQ=255)

                                                                     cTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCGACACATCg                                                                                         >  2:1086000/1‑136 (MQ=255)

                                                                      ttGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCtt                                                                                                                                  >  1:1701927/1‑94 (MQ=255)

                                                                      ttGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCtt                                                                                                                                  <  2:1701927/94‑1 (MQ=255)

                                                                        gAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCgggtgggt                                                                        <  1:1616583/150‑1 (MQ=255)

                                                                          aGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTAGTAGCGAGGGACTCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTga                                                                      <  1:635807/150‑1 (MQ=255)

                                                                           gTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTgaa                                                                     <  1:606151/150‑1 (MQ=255)

                                                                           gTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTgaa                                                                     >  1:1026070/1‑150 (MQ=255)

                                                                              cgcAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTgaagaa                                                                  <  1:545420/150‑1 (MQ=255)

                                                                              cgcAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGACACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTgaagaa                                                                  <  2:1243174/150‑1 (MQ=255)

                                                                                 aaTCCTTGCCCTCGCCCACTCACGCATCTCTACCGCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTgaagaagaa                                                               <  2:1557674/150‑1 (MQ=255)

                                                                                      ttGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCgggtg                                                                           <  1:1307356/133‑1 (MQ=255)

                                                                                      ttGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCgggtg                                                                           >  2:1307356/1‑133 (MQ=255)

                                                                                         cccTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCa                                                       <  2:1026070/150‑1 (MQ=255)

                                                                                         cccTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCa                                                       >  2:1101100/1‑150 (MQ=255)

                                                                                          ccTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAg                                                      >  2:1152191/1‑150 (MQ=255)

                                                                                          ccTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAg                                                      >  1:1511200/1‑150 (MQ=255)

                                                                                          ccTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTACACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAg                                                      >  2:66491/1‑150 (MQ=255)

                                                                                                  aCTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTcgcgcg                                              >  2:1518609/1‑150 (MQ=255)

                                                                                                  aCTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGAACCCAAGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTcgcgcg                                              >  1:407868/1‑150 (MQ=255)

                                                                                                   cTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCgg                                             >  1:685492/1‑150 (MQ=255)

                                                                                                   cTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCgg                                             >  1:685482/1‑150 (MQ=255)

                                                                                                    tCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAActc                                                            >  2:560592/1‑134 (MQ=255)

                                                                                                    tCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAActc                                                            <  1:560592/134‑1 (MQ=255)

                                                                                                      aCGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTa                                          >  2:647725/1‑150 (MQ=255)

                                                                                                      aCGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACAGCCGGGTGGGTgaagaagaa                                                               >  1:1999845/1‑129 (MQ=255)

                                                                                                      aCGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACAGCCGGGTGGGTgaagaagaa                                                               <  2:1999845/129‑1 (MQ=255)

                                                                                                        gCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTaaa                                        <  2:1110042/150‑1 (MQ=255)

                                                                                                        gCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTaaa                                        <  1:1518609/150‑1 (MQ=255)

                                                                                                        gCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTaaa                                        >  1:1928271/1‑150 (MQ=255)

                                                                                                          aaCTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGGTCGCGCGGGt                                           >  2:1624359/1‑145 (MQ=255)

                                                                                                          aaCTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGt                                           <  1:1624359/145‑1 (MQ=255)

                                                                                                              ctACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTtct                                  <  2:850544/150‑1 (MQ=255)

                                                                                                               tACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTtctc                                 <  1:1152191/150‑1 (MQ=255)

                                                                                                                aCCTCACTGACAGCATGCCCCGGGCGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCg                                                                                         >  2:1713363/1‑93 (MQ=255)

                                                                                                                aCCTCACTGACAGCATGCCCCGGGCGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCg                                                                                         <  1:1713363/93‑1 (MQ=255)

                                                                                                                 ccTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTcgc                               >  2:1563671/1‑150 (MQ=255)

                                                                                                                 ccTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGACGGGTTAGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTcgc                               <  1:1573389/150‑1 (MQ=255)

                                                                                                                  cTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCCGGGCACGCCAATCACCTGCACATCGGCCACCGCCgggtgggt                                                                        >  2:1681377/1‑108 (MQ=255)

                                                                                                                  cTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATc                                                                                                     >  1:311534/1‑79 (MQ=255)

                                                                                                                  cTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATc                                                                                                     <  2:311534/79‑1 (MQ=255)

                                                                                                                  cTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCgggtgggt                                                                        <  1:1681377/108‑1 (MQ=255)

                                                                                                                   tCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTtctc                                 >  2:877620/1‑146 (MQ=255)

                                                                                                                   tCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTtctc                                 <  1:877620/146‑1 (MQ=255)

                                                                                                                     aCTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCggg                                            <  1:1166126/133‑1 (MQ=255)

                                                                                                                     aCTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCggg                                            >  2:1166126/1‑133 (MQ=255)

                                                                                                                       tGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAActcctc                                                         <  1:1850323/118‑1 (MQ=255)

                                                                                                                       tGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAActcctc                                                         >  2:1850323/1‑118 (MQ=255)

                                                                                                                             cATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGa                   >  2:909373/1‑150 (MQ=255)

                                                                                                                               tggcctgggTGCGGCTGCACCCGGGGCATGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATc                 >  2:1580412/7‑150 (MQ=21)

                                                                                                                                  cccGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATg              >  2:221880/1‑150 (MQ=255)

                                                                                                                                   ctgggTGCGGCTGCACCCGGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTgaagaa                                                                  >  1:2150648/3‑97 (MQ=38)

                                                                                                                                   ctgggTGCGGCTGCACCCGGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTgaagaa                                                                  <  2:2150648/95‑1 (MQ=38)

                                                                                                                                   ccGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCa                                                                                                             <  2:1479515/54‑1 (MQ=255)

                                                                                                                                   ccGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGTACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGt             <  1:2274792/150‑1 (MQ=255)

                                                                                                                                   ccGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGt             >  2:620218/1‑150 (MQ=255)

                                                                                                                                     gggTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGTcc           <  2:1928271/150‑1 (MQ=255)

                                                                                                                                            gCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGTCCTtgctgc    >  2:1898829/1‑150 (MQ=255)

                                                                                                                                              cGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGTCCTTGCTgcgc  <  1:1679370/150‑1 (MQ=255)

CCGGAATTTCTGCACCTTGCCCGTCACCGTCATCGGGAACTCGTCGACAAAACGGAAATACCGGGGCACCTTGAAGTGCGCAATCCTTGCCCTCGCCCACTCACGCAACTCTACCTCACTGACAGCATGCCCCGGGTGCAGCCGCACCCAGGCCACGATCTCTTCACCGTACTTGCTGCAGGGCACGCCAATCACCTGCACATCGGCCACCGCCGGGTGGGTGAAGAAGAACTCCTCCAGCTCGCGCGGGTAAATGTTCTCGCCGCCGCGAATGATCATGTCCTTGCTGCGC  >  NC_002947/3920175‑3920466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`