BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,996,944	A→C	5.4%	M88R (ATG→AGG)	PP_3525 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,996,944	0	A	C	5.4%	326.0 / 9.4	112	M88R (ATG→AGG)	PP_3525	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (52/54); new base C (2/4); total (54/58)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.03e-01

Only 100 of 114 total aligned reads displayed.

CCAGCACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTGTAGGC  >  NC_002947/3996795‑3997089

ccAGCACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCa                                                                                                                                                   >  1:1611487/1‑150 (MQ=255)

ccAGCACGCCCTGCTGCAGTTGCAACCACGGCACCTGGCCCTCCTGCCCGGGGTAGCGGCTGGCCGCTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACCGTTTGATGATCAGCGCAGGCa                                                                                                                                                   <  2:117566/150‑1 (MQ=255)

 cAGCACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCAt                                                                                                                                                  >  1:1182465/1‑150 (MQ=255)

 cAGCACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCAt                                                                                                                                                  >  2:1991906/1‑150 (MQ=255)

 cAGCACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCAt                                                                                                                                                  >  2:2190109/1‑150 (MQ=255)

   gCACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATgc                                                                                                                                                <  1:249070/150‑1 (MQ=255)

   gCACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCATGACGCCTGGACCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATgc                                                                                                                                                <  2:1736895/150‑1 (MQ=255)

    cACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATgcg                                                                                                                                               >  2:1641832/1‑150 (MQ=255)

     aCGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATgcgc                                                                                                                                              <  1:1772490/150‑1 (MQ=255)

        cccTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGa                                                                                                                                           <  2:1182465/150‑1 (MQ=255)

         ccTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCATGCCTGCGCTGat                                                                                                                                          <  1:329701/150‑2 (MQ=255)

         ccTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCATGCCTGCGCTGat                                                                                                                                          <  1:330589/150‑2 (MQ=255)

         ccTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATgc                                                                                                                                                >  1:2328677/1‑144 (MQ=255)

         ccTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATgc                                                                                                                                                <  2:2328677/144‑1 (MQ=255)

         ccTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGaa                                                                                                                                          >  2:1463437/1‑150 (MQ=255)

         ccTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGaa                                                                                                                                          <  2:1809836/150‑1 (MQ=255)

          ctgctgCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAAt                                                                                                                                         <  1:428245/150‑1 (MQ=255)

              tgcAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCg                                                                                                                                     <  2:1511957/150‑1 (MQ=255)

              tgcAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCg                                                                                                                                     <  1:1122011/150‑1 (MQ=255)

              tgcAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCg                                                                                                                                     <  1:1000940/150‑1 (MQ=255)

               gcAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATg                                                                                                                                        <  2:188347/146‑1 (MQ=255)

               gcAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATg                                                                                                                                        >  1:188347/1‑146 (MQ=255)

                  gTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCa                                                                                                                                 <  2:1077491/150‑1 (MQ=255)

                   ttGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGGCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCAc                                                                                                                                >  1:166365/1‑150 (MQ=255)

                       aagcACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTTGGCCACGAAt                                                                                                                            >  2:2145975/4‑150 (MQ=255)

                        aCCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATa                                                                                                                           >  2:657687/1‑150 (MQ=255)

                         ccACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATAc                                                                                                                          >  2:2165093/1‑150 (MQ=255)

                         ccACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATAc                                                                                                                          <  2:2128415/150‑1 (MQ=255)

                           aCTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCg                                                                                                                                     <  1:2140932/137‑1 (MQ=255)

                           aCTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCg                                                                                                                                     >  2:2140932/1‑137 (MQ=255)

                           aCTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAg                                                                                                                        >  2:725447/1‑150 (MQ=255)

                            cTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATgcgc                                                                                                                                              >  2:1376999/1‑127 (MQ=255)

                               cACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAgcggc                                                                                                                    >  1:962359/1‑150 (MQ=255)

                                  cTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGGAAGAGCGCAAGTGCGAACAGTTTTCTCATCCACCCAGCAATGCCCTGCATGTCGGCCACGAATACAGCGGcgcg                                                                                                                 >  1:1571771/1‑150 (MQ=255)

                                    ggCCCTTCTGCCCGGGGGACCGGCTGGCCACTCGCCCACCACGGCCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGCCCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTg                                                                                                               >  2:472947/1‑150 (MQ=255)

                                     gCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGc                                                                                                              <  1:857731/150‑1 (MQ=255)

                                           ctGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAg                                                                                                        <  1:2187314/150‑1 (MQ=255)

                                           ctGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAg                                                                                                        <  1:616454/150‑1 (MQ=255)

                                              cccGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTa                                                                                                     <  2:401719/150‑1 (MQ=255)

                                                cGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGc                                                                                                   >  2:1295419/1‑150 (MQ=255)

                                                    gTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCtt                                                                                               <  2:2040838/150‑1 (MQ=255)

                                                     tACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTg                                                                                              <  1:1509102/150‑1 (MQ=255)

                                                     tACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTg                                                                                              <  2:724344/150‑1 (MQ=255)

                                                      aCCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTgg                                                                                             <  1:1258643/150‑1 (MQ=255)

                                                          gCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCt                                                                                                             <  1:1903533/130‑1 (MQ=255)

                                                          gCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGgctg                                                                                         <  1:1463437/150‑1 (MQ=255)

                                                          gCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGgctg                                                                                         >  2:2012638/1‑150 (MQ=255)

                                                               ccACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCgg                                                                                    >  1:7907/1‑150 (MQ=255)

                                                                cACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGc                                                                                   <  2:1588302/150‑1 (MQ=255)

                                                                         aTCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGt                                                                                                                <  2:311294/112‑1 (MQ=255)

                                                                         aTCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGt                                                                                                                >  1:311294/1‑112 (MQ=255)

                                                                          tCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTgccgcc                                                                         <  1:1446564/150‑1 (MQ=255)

                                                                           cACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCt                                                                        <  1:2012638/150‑1 (MQ=255)

                                                                            aCGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCtt                                                                       <  1:1152237/150‑1 (MQ=255)

                                                                            aCGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCtt                                                                       <  1:2190109/150‑1 (MQ=255)

                                                                                tCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTa                                                                                                     >  1:858725/1‑116 (MQ=255)

                                                                                 cGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCa                                                                  >  2:1917968/1‑150 (MQ=255)

                                                                                  gAGCAGCCCCAGGCTGTTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAg                                                                 <  2:426724/150‑1 (MQ=255)

                                                                                   agcagcACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGc                                                                <  1:472947/150‑1 (MQ=255)

                                                                                    gcagcaCCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGACACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCt                                                               >  2:1239067/1‑150 (MQ=255)

                                                                                     cagcaCCCGGTAGCTGTAATCGCGCATGGCCGGGTCTTCGAACTTTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCtt                                                              <  2:1716382/150‑1 (MQ=255)

                                                                                     cagcaCCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCtt                                                              <  1:657687/150‑1 (MQ=255)

                                                                                      agcaCCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTg                                                             <  2:7907/150‑1 (MQ=255)

                                                                                          cccGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCAc                                                         >  1:2077614/1‑150 (MQ=255)

                                                                                           ccGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCt                                                                                                                                             <  2:304027/65‑1 (MQ=255)

                                                                                           ccGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCt                                                                                                                                             >  1:304027/1‑65 (MQ=255)

                                                                                           ccGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCAcc                                                        >  2:1658958/1‑150 (MQ=255)

                                                                                                   tGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGa                                                                     >  1:372065/1‑129 (MQ=255)

                                                                                                   tGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGa                                                                     <  2:372065/129‑1 (MQ=255)

                                                                                                   tGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGt                                                      <  1:105879/144‑1 (MQ=255)

                                                                                                   tGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGt                                                      >  2:105879/1‑144 (MQ=255)

                                                                                                   tGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCatat                                                >  2:570830/1‑150 (MQ=255)

                                                                                                   tGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCatat                                                >  2:396857/1‑150 (MQ=255)

                                                                                                   tGTAATCGCGCATGACCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGGGTCCAGGTAGCCCTGGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCatat                                                >  1:530168/1‑150 (MQ=255)

                                                                                                            gcATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATATAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGCCCATATCGCGCGcca                                       >  2:1629612/1‑150 (MQ=255)

                                                                                                                  ccGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCATTAGTa                                 >  1:2146974/1‑150 (MQ=255)

                                                                                                                      gACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGccacca                                    >  2:2226841/1‑143 (MQ=255)

                                                                                                                      gACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGccacca                                    <  1:2226841/143‑1 (MQ=255)

                                                                                                                       aCCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATc                            <  2:1891462/150‑1 (MQ=255)

                                                                                                                       aCCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATc                            >  1:537267/1‑150 (MQ=255)

                                                                                                                          gcgAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCTACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGGGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGc                         <  1:331950/150‑1 (MQ=255)

                                                                                                                            gAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGGACCATGTCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCGCCGTCCATATCGCGCGCCACCAGTAAAATCTGCgt                       >  1:1103065/1‑150 (MQ=255)

                                                                                                                            gAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTcc                                                    <  2:745152/121‑1 (MQ=255)

                                                                                                                            gAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGATGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGcca                                       >  2:2021456/1‑134 (MQ=255)

                                                                                                                            gAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGATGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGcca                                       <  1:2021456/134‑1 (MQ=255)

                                                                                                                                   ttGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCa                <  1:725447/150‑1 (MQ=255)

                                                                                                                                       ggATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAACTCTGCGTGTCGGCACCaa            <  1:109052/149‑1 (MQ=255)

                                                                                                                                       cattCAGCGCATGCCTGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGgct                                                                                          <  1:162682/69‑1 (MQ=38)

                                                                                                                                       cattCAGCGCATGCCTGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGgct                                                                                          >  2:162682/4‑72 (MQ=38)

                                                                                                                                        gATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCg                                                                                     <  1:1868157/76‑1 (MQ=255)

                                                                                                                                        gATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCg                                                                                     >  2:1868157/1‑76 (MQ=255)

                                                                                                                                        attCAGCGCATGCCTGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGccacca                                    >  1:1507963/3‑125 (MQ=255)

                                                                                                                                        attCAGCGCATGCCTGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGccacca                                    <  2:1507963/123‑1 (MQ=255)

                                                                                                                                         aTCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAgg          >  2:2051746/1‑150 (MQ=255)

                                                                                                                                             gcgcAGGCATGTGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCTTCCATATCGCGCGccacca                                    <  2:811322/120‑1 (MQ=255)

                                                                                                                                             gcgcAGGCATGTGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCTTCCATATCGCGCGccacca                                    >  1:811322/1‑120 (MQ=255)

                                                                                                                                              cgcAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTGTa     >  2:1496742/1‑150 (MQ=255)

                                                                                                                                              cgcAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTGTa     <  2:2077614/150‑1 (MQ=255)

                                                                                                                                                 aGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTGTAGGc  >  2:1543265/1‑150 (MQ=255)

                                                                                                                                                 aGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTGTAGGc  >  2:1898027/1‑150 (MQ=255)

CCAGCACGCCCTGCTGCAGTTGCAACCACTGCACCTGGCCCTCCTGCCCGGGGTACCGGCTGGCCACTCGCCCATCACGGTCGAGCAGCACCCGGTAGCTGTAATCGCGCATGGCCGGGACCGCGAACAGTTTGCTGATCAGCGCAGGCATGCGCTGAATGTCGGCCACGAATACAGCGGCGCGTGCTTCCAGGTAGCCCTTGGGCTGCTCGGCCAGTGCCGCCTTGACCAGCTTGGCACCGTCCATATCGCGCGCCACCAGTAAAATCTGCGTGTCGGCACCAAGGCTGTAGGC  >  NC_002947/3996795‑3997089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`