| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_LN832404 | 4,023,909 | T→G | 100% | intergenic (+208/+173) | EK12AG100_RS19960 → / ← fadA | NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_LN832404 | 4,023,909 | 0 | T | G | 79.2% | 42.3 / 8.3 | 24 | intergenic (+208/+173) | EK12AG100_RS19960/fadA | NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA |
| Reads supporting (aligned to +/- strand): ref base T (5/0); new base G (10/9); total (15/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGAT > NZ_LN832404/4023625‑4024192 | ttGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAACTAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCCTCCGGCAACTGGTGCATGATGCCTGATGCGCCCCTGGCGCGGCt > 2:522701/1‑292 (MQ=255) gcgATCTTTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATcag < 1:145972/292‑3 (MQ=255) gcgATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATTACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATcag > 1:441864/1‑290 (MQ=255) aTCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGTCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACAGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCTCGTCTTATcaggcc > 1:102110/1‑287 (MQ=255) tGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACattgg < 1:467943/292‑5 (MQ=255) gCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACattggt < 1:315244/292‑6 (MQ=255) gCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACattggtgccggat > 1:387768/1‑280 (MQ=255) gCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTCGGCCGGATCAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGt > 1:731433/1‑292 (MQ=18) tGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGttca > 1:234276/1‑290 (MQ=18) ttGGCGAAGCGTTTGCATTTATCTGAGAAATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcgc < 1:366242/292‑1 (MQ=18) ggCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcgccg > 1:542120/1‑292 (MQ=18) tGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATcc < 1:136422/292‑1 (MQ=18) tGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATcc > 1:299922/1‑292 (MQ=18) tttgttaTTTAGCTTAGTGATAAAAAAACCCGCCCCTTGCAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGAGCTATGACGTAGCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCGTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGc < 2:780562/286‑1 (MQ=14) aTTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATCAGGCGTTTACGCCGCCTCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTACCAGGCCTAC‑CTTGGTGCCGGATCGGTAGACCGCCTAAGGCGATCACGCCGAATCCGGCaggtgg > 1:607211/1‑287 (MQ=17) tttATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGt > 1:543537/1‑292 (MQ=17) ttATCGGAGATAGAAAAAGACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGGTATTCAGTGGCATTTAGATCTATTACGTAGCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACTCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGtt < 2:441864/292‑2 (MQ=14) gagaTATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGttaaaccc < 2:234276/292‑8 (MQ=14) gCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCt < 2:283834/200‑1 (MQ=255) gCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCt > 1:283834/1‑200 (MQ=255) gtatctggcaaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTCAACCCGCTCCAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCCGACCAAACTGAACGCCTTTGCGTTCCAt > 1:328008/15‑292 (MQ=255) gtatctggcaaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGGTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGCCTTTGCGTTCCAt > 1:456412/15‑292 (MQ=255) ctggcaaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCCCAAACACCGTCGCAATACCCTGACCCACAACGAGACACACCCTCCGCAGCCCAAACCGACCGTCCTTGCGGTCCATCaaa > 2:323647/11‑290 (MQ=18) gATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATCCACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGc > 1:702934/1‑292 (MQ=255) gctgcGCTGTGTCGTCTGTGCGTGCCTACAAATAGT‑CC‑GAACCGTGGGCCGGATAAGGCGGTTGCGCCGCATCCGGCAATTGGTGCATGATGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTACATGGGGGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTTGTGCTGa < 2:456412/288‑1 (MQ=37) cGACGCTGTCGCGTCTTACCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCCACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCCTCCGGCAAGCGGTTAAACCCGCTCAAACACCCTCGCAATACCCCGACCCAGCCCGTTACACATCCCCGCCAGACCCAACTGAACGTCCTCCCCTTCCATCAGACTCAGCAGCGTGGGGCTGAt > 2:28836/1‑292 (MQ=25) | TTGCCCGCGATCTGTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGAT > NZ_LN832404/4023625‑4024192 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |