Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 3,204,060:1 +G 86.5% pseudogene (177/605 nt) ttdB → L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324043,204,0601.G86.5% 162.6 / 21.2 52pseudogene (177/605 nt)ttdBL(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base . (4/3);  new base G (22/23);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

ATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCG  >  NZ_LN832404/3203769‑3204349
                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                   
aTCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTACCCGCTgga                                                                                                                                                                                                                                                                                                  >  1:615073/1‑293 (MQ=255)
  ccgtccccctccGGCGTGGCTGTCGCTGCGGGCTGGTGGGGGCAGCGTCGCGGCACGCTGCTGGTGCAGGCCGATCTCACCTTTGAAAATCGGTCTCACACCCGGGGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGAGCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGgccc                                                                                                                                                                                                                                                                                                <  2:765495/284‑1 (MQ=255)
              ggCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGAACTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCaa                                                                                                                                                                                                                                                                                     >  1:357102/1‑295 (MQ=255)
                            tACCGGCTGCTGGGCGCATCGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAACAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACCTCCGAACCCTTTCGATCCCAACGGCACAGCGATTTTCCCCGCCGGCCCCATCCGGCGCCAAAACGGCGCCAAAt                                                                                                                                                                                                                                                                       >  2:580619/1‑295 (MQ=255)
                                 gctgctGGGCGCATCGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGgag                                                                                                                                                                                                                                                                  >  1:234170/1‑295 (MQ=255)
                                  ctgctgGGCGCATCGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGgaga                                                                                                                                                                                                                                                                 <  1:445589/295‑1 (MQ=255)
                                   tgctgGGCGCATCGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGCTCCCTTACGATCTCACCGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAACCGGCGACAAATggacaat                                                                                                                                                                                                                                                                >  2:621349/1‑290 (MQ=255)
                                   tgctgGGCGCATCGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGAt                                                                                                                                                                                                                                                                >  1:73762/1‑295 (MQ=255)
                                   tgcgttgcgcAGCGGCGCGTCACGCTGCTGGTTCATGCCGATCTCACCTGTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGAt                                                                                                                                                                                                                                                                <  2:641362/289‑1 (MQ=255)
                                      gcggCGCATCGTCGCGGCACCCTGCGGGTTCATGCCGATCCCACCTTTGAAAATCGGTCCCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCGCAAGATCTGCAAGATATGCGCGTCGGCGATGTGATCTACCTGACCGGTACGCGGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGt                                                                                                                                                                                                                                                             <  1:112854/293‑1 (MQ=255)
                                          gtcATCGCCGCGTCACTCTGCTGGTTCAAGCCGAGCACACCTGTGAAAATCGGTCTCACAACCTGAGCGCGTTATGAAAAAGATCCGAACAACCCCGATCAAAGCGGAAGATCTGCAAGATAGTCGGGTCGGCGAGGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTcc                                                                                                                                                                                                                                                         <  2:599331/293‑1 (MQ=255)
                                                     ggCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGCCCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGCCCCCTCGTGCGCACAAACGGCGACAAATGGGAGCTGGTCTCCGGCGGCCCGACa                                                                                                                                                                                                                                             >  2:512300/1‑295 (MQ=255)
                                                                       gCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAACCGTCTGATCCCTCACGATCTCAACGGCAAAGCGCTTTTCCACGCTGGCCCCACCGTTCACAAAAACCGCCACAAATGGGGGCTGGTCCCCGTCCGCCCGACCACCAGTacgagaagggt                                                                                                                                                                                                                            >  2:393874/1‑285 (MQ=255)
                                                                           tatccACCTTTTAAAACCGTTCTACCACCCTGTGCACGTTATGATAAAGATCCTGACAACCCCGTGCACAGCTGAGTATTTGCAAGATATTCGCTTCGGCGATGTGTGCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTGGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACTATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGt                                                                                                                                                                                                                        <  2:768986/291‑1 (MQ=255)
                                                                                 ccTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGTTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGaa                                                                                                                                                                                                                  >  1:212130/1‑295 (MQ=255)
                                                                                  cTTTGAAAATCTGTCGCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAAc                                                                                                                                                                                                                 <  1:32558/295‑1 (MQ=255)
                                                                                        aaactgccctgccacCCGGCGCGCTGTATGAAAAAGCGCCTGCCAACCCCGATCAAAGCTGAAGATCTGCAATATGTTCGCGTCGGCTGTGTGATCTACCGGACCGGCACGCGGGGGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCACCGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAAt                                                                                                                                                                                                           <  2:666573/283‑1 (MQ=255)
                                                                                            cgccctcACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGCCGAGGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCTACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTAt                                                                                                                                                                                                       <  1:382237/291‑1 (MQ=255)
                                                                                            cTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTAt                                                                                                                                                                                                       <  1:560813/295‑1 (MQ=255)
                                                                                               tctcACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGCTTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTCTGCGGATGGAACGTTTTGAAGGTGAATTTAGTgc                                                                                                                                                                                                    >  2:473621/1‑294 (MQ=255)
                                                                                                        cGGAGCGCGTTATTAAAAAGATCCTTACAACCCCGAACAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGc                                                                                                                                                                                          <  1:211800/295‑1 (MQ=255)
                                                                                                                gTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAAc                                                                                                                                                                                   >  1:79466/1‑295 (MQ=255)
                                                                                                                    tGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACtggtg                                                                                                                                                                              <  1:667470/295‑1 (MQ=255)
                                                                                                                        aaaGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGGGGtt                                                                                                                                                                           >  1:698469/1‑295 (MQ=255)
                                                                                                                          aagcccTGTCAACCCCGTCCAAGTCGGAGGTTCTCCAAGATTTGCGGTTCGGCGATGTGATTTACCTGACCGGTACGCTGGGGACCTGCCGCGACGTTGGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTgg                                                                                                                                                                         <  2:603070/291‑1 (MQ=255)
                                                                                                                                 taCAACCCCGTTCAAAGCTGTAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTTACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGAGCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAggt                                                                                                                                                                  <  1:638549/294‑1 (MQ=255)
                                                                                                                                           aTCAACGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGc                                                                                                                                                        <  1:272464/295‑1 (MQ=255)
                                                                                                                                           aTCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGc                                                                                                                                                        <  1:621349/295‑1 (MQ=255)
                                                                                                                                            ctcttccgatctaTCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGcc                                                                                                                                                       <  1:208760/283‑1 (MQ=255)
                                                                                                                                             cAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCCAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTACGCGTCTGGAAAGTGTGGAACGTGAATTTATTGCGCAGACCGGCGTGAAACTGTGGGTTGGCAAAGGGGGGATGGGGCCg                                                                                                                                                      >  2:131959/1‑295 (MQ=255)
                                                                                                                                                agcataaGATCTGCGAGATATTCGCGTCGGCGATGTGATCTACCTAACCGGGACGCTGGTGACCTGCCGCGACGTTTGGCACCGCCGGTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTg                                                                                                                                                   <  2:465195/290‑1 (MQ=255)
                                                                                                                                                        aTCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAACAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGACTGGAAAGGTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAAACGGGGGGTGGGAAGGGTGGGATgggggcaggcagggagga                                                                                                                                           >  2:208760/1‑281 (MQ=255)
                                                                                                                                                                         gTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAACTGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTCTGGAAAGTTTTGAAAGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCACAGGTGGTATGGGGCCGCTTACCGAAGACGGCTGCCAGaaactta                                                                                                                          >  2:298997/1‑291 (MQ=255)
                                                                                                                                                                         gTCGGCGATGTGAGTTTCCTGACCGGTACGCGGGGGACCAGCCACGACGGTGGTCACCGCCGTTTTATCGAACGGAAACGTCCGATCCCTTACGATATCAACGGCAAAGCGGTTTTCCACGCTGGCCCCATCGTGCGCAAAGACGGCGACAAATGGGGGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCa                                                                                                                          <  2:331311/295‑1 (MQ=255)
                                                                                                                                                                               gATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGgcgc                                                                                                                   >  1:652310/1‑295 (MQ=255)
                                                                                                                                                                               gATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCt                                                                                                                  >  1:606844/1‑295 (MQ=255)
                                                                                                                                                                                    gATCTACCTGACCGGGACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAt                                                                                                              <  1:564078/295‑1 (MQ=255)
                                                                                                                                                                                      tCTACCGGACCGGTACGCTGGTGACCTGCCCGGACGGTTGTAACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTAAACGGAAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACAtgt                                                                                                            <  2:234170/295‑1 (MQ=255)
                                                                                                                                                                                                    aCGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGGGAAACTGGTGGTTGGCACAGGTTGTATGGGGCCGCTGCCCGGAGAAGCAGGCCAAACATTCCAGGCCGCCACACTGATTTTCCCggtat                                                                                              >  2:434278/1‑292 (MQ=255)
                                                                                                                                                                                                     cGCTGGTTACCTGCGTCGACGTTGTTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAgg                                                                                             <  1:382149/295‑1 (MQ=255)
                                                                                                                                                                                                                  ccGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTgg                                                                               >  1:140218/1‑295 (MQ=255)
                                                                                                                                                                                                                           ttGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAcc                                                                       <  1:381850/295‑1 (MQ=255)
                                                                                                                                                                                                                              tCACCGCCGTTTGATCGAACTGAAACTTCCGATCCCCTACGATCGCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGAAAAATGGGGGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAg                                                                    <  2:73762/295‑1 (MQ=255)
                                                                                                                                                                                                                                            tCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGAGGCAACCCAGGTGGAAGAGATTga                                                      >  1:601594/1‑295 (MQ=255)
                                                                                                                                                                                                                                              gAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGACGAGATTgaaga                                                   >  1:184814/1‑295 (MQ=255)
                                                                                                                                                                                                                                                  tGAAACGTCCGAGCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGt                                                <  1:471422/295‑1 (MQ=255)
                                                                                                                                                                                                                                                   gAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGCTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCCGGCTGCGCGGTGCTGGCGGCAACCCATGTGGCAGAGATTGAAGAAGTg                                               >  1:224585/1‑295 (MQ=255)
                                                                                                                                                                                                                                                     aaCGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGACGAAGTGCa                                             >  1:402184/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                ccTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGc                                 >  1:532063/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                     cGACCTCAACGTCAAAGCGATATTCCACGCTGCCCCATCGTGCGGAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGaa                           <  2:297046/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                           cAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGACACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTTCTGGCGGCCACCCAGGTGGCAGGGATTGAAGAAGTTCACTGGACCGGGCtagtgattacg                      >  2:502885/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                           aaaCGGCAAAGCGGTTGTCTCCCCTGCCCCAACGTGCGCAAAAACGGGGACAAAGGGGAGAGGGTTTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTGTTGAACGTGAAGTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCgg                     <  2:344455/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                            aaCGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGAAGGCTGCGCGGTGCTGGCGGCAACCCAGGGGGCAGTGGGTGAAGAAGTGCACTGGACCAGACTCGGAAGGCAgg                     >  2:436395/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                gCAAAGCGATTTTCCCCGCTGGCCCCATCGTGCGCAAAAAGGGCTAAAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTc                 <  2:698469/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                gCAAAGCGATTTTCCACGCTGGCCCAATCGGGCGCAAAAACGGCGACAAATGGGGGATGGTCTCGGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTc                 <  2:55960/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   aaGCTATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACt              <  1:209790/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     gCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCCACCCAGGCGGAAGACATTTAAGAAGTGCACTGGACCGAGCTCGGACGGCCGGAGTcgcggt            >  2:260188/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                          tttCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCCCGGCATGCCGGAGTCACTGTGGGTc       >  2:345372/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                          tttCCACGCTGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCt      <  2:140218/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               aCGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGGTTGGAAAAGTGCACTGGACAGAGCGCGGAAGGGCGCAGCAACGGTggggatggcg  >  2:52346/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                   
ATCCGTCAACCATCGGCGTTGCTGTCTCTACCGGCTGCTGGGCGCATCGTCGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTCACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCG  >  NZ_LN832404/3203769‑3204349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: