Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NZ_LN832404 | 3,724,623:1 | +C | 100% | coding (1261/1353 nt) | xylB ← | xylulokinase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_LN832404 | 3,724,623 | 1 | . | C | 93.7% | 214.9 / 8.5 | 63 | coding (1261/1353 nt) | xylB | xylulokinase |
Reads supporting (aligned to +/- strand): ref base . (3/1); new base C (34/25); total (37/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.37e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. |
TTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGA‑AACCAAACTGGCTCGGCAC > NZ_LN832404/3724329‑3724916 | ttAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACGTAGATATATAGATGGGAATTAGCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑t < 2:743859/295‑1 (MQ=255) tAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGTCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑c < 1:581298/295‑1 (MQ=255) aaaTGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCCCACGCCTTGCTGCGCCCCGTCCTGGCCCCACC‑T‑ccc‑cc > 2:176149/1‑295 (MQ=255) atgatgTTCAGAATAAATTCAGACTAAATATTAATAAAATACTGAGATATATAGAGGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAAGGGCAGAAGTGGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑ccc‑cc‑cc < 2:594282/295‑1 (MQ=255) aTGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑ccc‑cc‑cc > 1:567001/1‑295 (MQ=255) tttaaaaaTAAATTAATACTAAATATTAATTAATGACTGGGATATATAGATGGGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑ccc‑cc‑cccgtac < 2:710485/289‑6 (MQ=255) tCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACgg < 1:297916/295‑1 (MQ=255) aaaTTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATAGa > 1:336379/1‑295 (MQ=255) aaTTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAg < 1:736131/295‑1 (MQ=255) aaTTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑AAGGTAATCAAg > 1:499655/1‑295 (MQ=255) tCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAgctg > 1:384822/1‑295 (MQ=255) attaattaattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGa > 1:54753/1‑295 (MQ=255) attaattaCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCACCA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACAGCTGAAATc > 1:758102/1‑295 (MQ=255) cttcctatctagaTATATGGTTGTGAAGTGGCCCCCACCCGGTCAGGCAGGGGATAACTGTTACGCCATTAATGGCAGAGGTTGCTGTAAGAGGCGACGGAACGTGTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATcc < 2:66686/285‑1 (MQ=255) aCTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGc > 1:769129/1‑295 (MQ=255) gggtctaataGATGGAAGTAATCCCCCACCCGGTCAGGCGGGGGAGAAGGTTTACGCCATTAATGGCAGAAGTGGCGGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc < 2:392358/288‑1 (MQ=255) agaTATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCATCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATccagatcggaag > 1:66686/1‑285 (MQ=255) agaTATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGAGACTGTTCTAACGGTAGTTGCGGCACCAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑AAGGTAATCGAACTGCTGACCGCTGATATCAGCCCGCATCt > 1:761490/1‑295 (MQ=255) gaTATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTCACCGCTGATATCCGCCCGCGTCTg > 1:645928/1‑295 (MQ=255) atatatAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTgc > 1:211345/1‑294 (MQ=255) aaTGTGAATGTACCCCCACCCGGGCAGGCAGGGGAGAACGTTTACGCCATTAATGGCATAAGTTGGTGTTAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGt < 2:163749/294‑1 (MQ=255) aTGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCCACCTTGATGCGCCCAGTGCTGGCCCCACC‑T‑CCC‑CCACCCG‑ACGGCACCACAGCTGCTCACCGCTCATAGCAGCCAGCCTCCGACGCCAgac > 2:727681/1‑293 (MQ=255) tgtgAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCAAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc > 1:427112/1‑295 (MQ=255) aTCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGATGCTGACCGCTGATATCCGCCAGCATCTGCAGCCCGTACTCACTAcg > 1:578555/1‑295 (MQ=255) cccccACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgcg > 1:337598/1‑295 (MQ=255) tcccGGTCAGCCAGGTGGTACCTGTTACTCATTGAAGTGCTGAAGTGGTTGCTGGAGGCGACGGAACTTTGCTCGTCGTGGC‑TGTGAAGCTGCATAACGCTGCGCATCTGGTAGATGCGACTTTTCTAACGGTAGTTGCGGCAACAATTCAATTAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg < 2:690526/294‑1 (MQ=255) agtcaggGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑GGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGTCCCCAAt < 1:456519/292‑1 (MQ=255) ggcaggGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCTCCCAATc < 1:442102/295‑1 (MQ=255) caggGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATCTACGCCAGCATCAGACGCCAGTACTCCCTACGCGCCCCGCCAccaaccaa > 1:523986/1‑295 (MQ=255) gATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTCCGCGCCCCGCCCACCATCAACgaca > 1:515183/1‑292 (MQ=255) aTAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCCACC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGACATCCGACAGCATCTGCCGCCAGTACTCACTCCGCGCCCCGGCCACAATCAAAGTAac > 1:457014/1‑295 (MQ=255) cGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACCTAACACTTTGCGGt > 1:517182/1‑295 (MQ=255) aTTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCCTCCTCCGACGCCAGTACTCCCCACCCCCCCCGCCCCCCATCACCAGCACACCTTACGGttgca > 2:375330/1‑292 (MQ=255) aTGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTg > 1:202388/1‑218 (MQ=255) aTGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTg < 2:202388/218‑1 (MQ=255) gCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACACC‑CCC‑CC‑CCGT‑CCGGTAATCGAGCTGCTGACCGCCGATTTCCCCCAGCCTCCGCCCCCCGTACCCCCCACGCGCCCCCCCCCCACCCCACCTCACCCTTTCCGGTTTAAAAccccg > 2:52001/1‑292 (MQ=255) gCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAAGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa > 1:678033/1‑295 (MQ=255) aGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAgg < 1:731142/295‑1 (MQ=255) gAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc < 1:742480/295‑1 (MQ=255) gCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACACTTTTCGTGTTAATACCGCAGGCATGccc > 1:330764/1‑293 (MQ=255) cTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATCCCGCCGGCATGCACg > 1:86224/1‑295 (MQ=255) agagGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCCCGACATc > 1:747221/1‑295 (MQ=255) gagGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc < 1:347878/295‑1 (MQ=255) ggtgACGGACTGTGTCTCGTTGTGGCGTGGT‑AGCGGCATCGCGCTTCGCATCGGGTAGATGCTGCTGTTCTAACGCGAGTTGCGGCAACAATTCAATGTGCGATTTCTCTGGATTCGCCGCGATCTGCTCCATCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt < 2:83620/292‑1 (MQ=255) gACGGAACGTTGCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGcc < 1:106282/295‑1 (MQ=255) gAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCCCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt < 1:337491/295‑1 (MQ=255) cgtcgtGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACCATTTGCGGTTTAATACCGCAGGCATGCACGACATCCATTCCATCTGCCAGCGCAt > 1:422659/1‑295 (MQ=255) cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGcc < 1:176149/295‑1 (MQ=255) gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCCCGCCTTCCAGCACTGCTCGCGCCCGTTCATTGGGGCCAt > 1:119520/1‑295 (MQ=255) gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCCGCACTGCTCGCGCCAGTTCATTGGGGCCAt > 1:530616/1‑295 (MQ=255) tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGtt < 1:291070/295‑1 (MQ=255) ggtggtTTCGGCAACAATTCCATGAGCGAGTTCTCTGGATTCGCCGCGATCCGCGCCCGCCTTGCTGCGCCCAGTGCCGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCa < 2:384822/295‑1 (MQ=255) gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAAcc > 1:593587/1‑295 (MQ=255) tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCaa < 1:200487/295‑1 (MQ=255) gtcaccaaCTCATTGGGCGATTGCTCTGGATTCGCCGCGATCGCCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGG‑CCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGa < 2:530616/290‑1 (MQ=255) aaaagccatagggggATTTCTGTGGATTCGCCGCGGTCTGCGCCGGCCTGGCTGCGCCCAGGGCGTGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGGGCTGCTGACCGCTGATAACCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAAc < 2:645928/281‑1 (MQ=255) tCAAGGAGCGATTTCTCTGGATTCCCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCt < 1:595563/295‑1 (MQ=255) aTGAGCGACTTCTCTGTGTTCGCGCGGATCCGCGCCAGCCCTGCTGCGCCCAGCGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGTTCTCCGCAAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCg < 2:23796/295‑1 (MQ=255) aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCt > 1:71877/1‑295 (MQ=255) gCGATTTATCGGGATTCGCCGCGATCTCGGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAAGCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTg < 1:86164/295‑1 (MQ=255) gctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGattat < 1:494256/294‑1 (MQ=255) gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtg > 1:54039/1‑295 (MQ=255) gATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCCCCTGGGGATTCTtgtt > 1:60165/1‑294 (MQ=255) aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATtgtgt > 1:746759/1‑295 (MQ=255) gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGGGGCGTACGc > 1:298983/1‑295 (MQ=255) aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTc < 1:753607/295‑1 (MQ=255) tCTGCCCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTc < 1:375330/295‑1 (MQ=255) gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCACACCAAAGAAAACCCCCTTCGCCTGGGGAATATTGTGTGGCGTAACCTCGCCgg > 1:295160/1‑295 (MQ=255) gccctgacggcgcCCGGGTCTGCCCCCACG‑T‑ACC‑CC‑CCTG‑AGGTGAACCGACGTGCTGACCGCTGGTAGCCACCAGCAGCGGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGGTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAg < 2:74945/286‑1 (MQ=255) gCCTTGCTGCGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAACGAAAACCCCCTTCGCCTGGGGATTATTGTTTGGCGTACGCTCGCCggcacg > 1:561909/1‑291 (MQ=255) cctgtctgcGCCCCTTGCGGGCCCCCCA‑C‑CCC‑CC‑CCCTGCCGGTACTCGTGCTTCTGACCGCTGTTCTCCTCCAGCATCTGACGCCCGTACCCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGa < 2:678033/290‑1 (MQ=255) gctgcGCCCAGTGCTGGCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAg < 1:587040/295‑1 (MQ=255) ggCCCCACA‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGA‑AACCAAACTg < 1:341893/295‑1 (MQ=255) caca‑TCCCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCCCTTCTCGCGCCAGTTCATTGGGGCCATGTTGATGCGTCAAACCAACGAAAACCCCCTTCCACTGGGGATTATTTTGTGGCGTACCACAGCCGGCAAGAAAAGGCCGACAACCGAAcggcccg > 2:484973/1‑289 (MQ=255) a‑T‑CCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCCCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAACCCAAAGAAAACCCCCTTACCCTGGGGATTATTGTGTGGAGTACGCCCGCCGGAAGGAGAAAGACGA‑AAACAAAATGGCTACGCAc > 2:373919/1‑295 (MQ=255) | TTAACAAATGATTTTCAGAATAAATTCATACTAAATATTAATTAATTACTGAGATATATAGATGTGAATTATCCCCCACCCGGTCAGGCAGGGGATAACGTTTACGCCATTAATGGCAGAAGTTGCTGATAGAGGCGACGGAACGTTTCTCGTCGTGGC‑TGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑T‑CCC‑CC‑CCGT‑ACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCCAGCACTGCTCGCGCCAGTTCATTGGGGCCATGTTGATGAGTCAAACCAAAGAAAACCCCCTTCGCCTGGGGATTATTGTGTGGCGTACGCTCGCCGGAAAGATAAGGCAGA‑AACCAAACTGGCTCGGCAC > NZ_LN832404/3724329‑3724916 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |