Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	2,583,288	T→C	89.0%	V461A (GTA→GCA)	narQ →	nitrate/nitrite two‑component system sensor histidine kinase NarQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	2,583,288	0	T	C	89.0%	211.3 / 12.7	74	V461A (GTA→GCA)	narQ	nitrate/nitrite two‑component system sensor histidine kinase NarQ
Reads supporting (aligned to +/- strand):  ref base T (5/3);  new base C (36/29);  total (41/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

CCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGCTCT  >  NZ_LN832404/2582997‑2583577                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                   ccATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACt                                                                                                                                                                                                                                                                                                 <  1:748591/295‑1 (MQ=255)  aatgcgcggcgAGTTGCACGCAGCGCTGGATCAGGGACTTTGTGACTTAGGTGTCCAGTGGACGTGACGGAGTCGTTGGATACCGGAAGATAACGCCACCGAGCAAAGGATCATGGCCGATTTTGCCCAGGCGTTGAATGATGCTGATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTg                                                                                                                                                                                                                                                                                                <  2:758995/287‑1 (MQ=255)       cccGCGAATGGCACGACTCGCTGGCTCAGGTACGTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgc                                                                                                                                                                                                                                                                                           <  1:513415/295‑1 (MQ=255)         cgggAATTGCACGATTCGCTGGCTCAGGTACTTACTTACTTACGTATCCAGTTGACGTTACGGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATgcgc                                                                                                                                                                                                                                                                                         <  2:770064/292‑1 (MQ=255)          gcgAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCa                                                                                                                                                                                                                                                                                        >  1:29600/1‑295 (MQ=255)                     gACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCAAACCCTGGTACTGGATGCGCAAATGCAGGTGc                                                                                                                                                                                                                                                                             >  1:731218/1‑295 (MQ=255)                        tCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCAtt                                                                                                                                                                                                                                                                          >  1:37729/1‑295 (MQ=255)                              gCTCCGGTACTTTCTGACGTACGTAGCCAGTTGACGTTACTGAAGCGGTGGATACCGCAAGATAACGCCACCGCACAAGGGATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCGTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGc                                                                                                                                                                                                                                                                    <  2:669031/295‑1 (MQ=255)                                 cAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCaaa                                                                                                                                                                                                                                                                 <  1:289148/295‑1 (MQ=255)                                  aGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAat                                                                                                                                                                                                                                                                >  1:566569/1‑295 (MQ=255)                                         ttCTTACGTACGGATCAAGTTGCAGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTGTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTcgc                                                                                                                                                                                                                                                         <  2:279665/295‑1 (MQ=255)                                                   cGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGc                                                                                                                                                                                                                                               >  1:544866/1‑295 (MQ=255)                                                     tATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTg                                                                                                                                                                                                                                             >  1:276291/1‑295 (MQ=255)                                                      aTCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATGGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGa                                                                                                                                                                                                                                            <  1:660143/295‑1 (MQ=255)                                                          aGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGc                                                                                                                                                                                                                                        <  1:25263/295‑1 (MQ=255)                                                           gTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCCGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCg                                                                                                                                                                                                                                       >  1:13210/1‑295 (MQ=255)                                                             tGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGTCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGAt                                                                                                                                                                                                                                     <  1:761849/295‑1 (MQ=255)                                                                ctttccttacgCGTTATATACCGGACGCTACCGCCCCCGCACAAATTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGGGCTGTTGCCTACTTTCCGCCTGACGCTGA‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGaa                                                                                                                                                                                                                                  <  2:356866/285‑1 (MQ=255)                                                                    aCTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGAACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCac                                                                                                                                                                                                                              <  2:122609/295‑1 (MQ=255)                                                                       gAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTCCTGGATGCGCACATGCCAGTGCCTTTGTTTGAAATTTTTCCCAAAGCGGTGCATAATGCGAGGAAGCacgcc                                                                                                                                                                                                                           >  2:113750/1‑295 (MQ=255)                                                                        aaGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCacgcca                                                                                                                                                                                                                          <  1:474074/295‑1 (MQ=255)                                                                          gggtttgtaaacGGAAGGTAACGCCACCGCACAAAGAATCAGTGCCGATTTTTCCCAGGCGTGGAATGATGCTTATCGGCAGTTACTCGAGCTGGTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCacgccaac                                                                                                                                                                                                                        <  2:271896/284‑1 (MQ=255)                                                                                  tACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGa                                                                                                                                                                                                                >  1:700372/1‑295 (MQ=255)                                                                                     cGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAAt                                                                                                                                                                                                             >  2:345690/1‑295 (MQ=255)                                                                                     cGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAAt                                                                                                                                                                                                             >  1:135269/1‑295 (MQ=255)                                                                                      ggAAGATAAAGCCACCGCACAAAGTATAATGGCCGATGTTTCCCAGTCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATc                                                                                                                                                                                                            <  2:261305/295‑1 (MQ=255)                                                                                           aTAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGt                                                                                                                                                                                                       >  1:286169/1‑295 (MQ=255)                                                                                            tAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCCAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCCCGCCAACGCCCGCGAAACCGcccgc                                                                                                                                                                                                      >  2:687087/1‑292 (MQ=255)                                                                                               cGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTAAGt                                                                                                                                                                                                   >  1:418841/1‑295 (MQ=255)                                                                                                gCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGtt                                                                                                                                                                                                  <  1:634590/295‑1 (MQ=255)                                                                                                gCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGtt                                                                                                                                                                                                  <  1:127034/295‑1 (MQ=255)                                                                                                gCCAACGCACAAAGTATACTGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCGGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGtt                                                                                                                                                                                                  <  2:222080/295‑1 (MQ=255)                                                                                                     cGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTc                                                                                                                                                                                             >  1:30913/1‑295 (MQ=255)                                                                                                      gCACAAAGTATCATGGCCGAGTTGTCCCAGGCGTTGAATGATGCTTAACGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCa                                                                                                                                                                                            <  2:79545/295‑1 (MQ=255)                                                                                                        acaAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCAcc                                                                                                                                                                                          >  1:22310/1‑295 (MQ=255)                                                                                                         caAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTTACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGTGTCACcg                                                                                                                                                                                         <  1:453372/295‑1 (MQ=255)                                                                                                            aGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCgcg‑c                                                                                                                                                                                     >  1:267467/1‑295 (MQ=255)                                                                                                             gTATCATGGCCGATTTTTCCCAGGCGTTGAATTATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑cc                                                                                                                                                                                    <  1:92457/295‑1 (MQ=255)                                                                                                             gTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑cc                                                                                                                                                                                    <  2:320007/295‑1 (MQ=255)                                                                                                              tATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCg                                                                                                                                                                                   >  1:700190/1‑295 (MQ=255)                                                                                                                  aTGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTGGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCACCGCCAGCGAAATCGCCGGCAGTTGCGTCAACGCG‑ACGGCCg                                                                                                                                                                               >  1:209038/1‑295 (MQ=255)                                                                                                                    ggCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGc                                                                                                                                                                             >  1:684695/1‑295 (MQ=255)                                                                                                                        gATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACAAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGAATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATAGCAGTCAGTTGAGTCA‑CGCGACCGGACGGAAAta                                                                                                                                                                         >  1:756655/1‑294 (MQ=255)                                                                                                                                      gTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGCTTCCGAATGCGATGAAGCACGCCAACGCACGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGCCGGCCACCCCACGGTGTATATc                                                                                                                                                           >  2:122122/1‑295 (MQ=255)                                                                                                                                       ttGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATcc                                                                                                                                                          <  1:687087/295‑1 (MQ=255)                                                                                                                                       ttGAATGAGGCTGATCGGCAGTTACGCGAGCTGTTGACGACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATcc                                                                                                                                                          <  1:38619/295‑1 (MQ=255)                                                                                                                                         gAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCCCGCCAACGCCAGCGAAATCGCCGTCCGTTGCGTCACCGCG‑CCGGACGGCCATCCACCGGTTtctaaacgt                                                                                                                                                        >  2:319542/1‑290 (MQ=255)                                                                                                                                           atgatgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCCCCGCG‑CCGGCCGGCAATCCCCCGGtttcactcctgtc                                                                                                                                                      >  1:620655/1‑285 (MQ=255)                                                                                                                                                gctaaacGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACg                                                                                                                                                 <  1:480877/289‑1 (MQ=255)                                                                                                                                                   tATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCCCCGCG‑CCGGCCGGCAACCACACGGTTTATCTCCGTGATACCGGGa                                                                                                                                              >  2:97762/1‑295 (MQ=255)                                                                                                                                                          cgTTCCGCGGGCGGTTGACTACGTTCCGCCGGACGGTGA‑AGCAGGCGGATCTCCCCGCCGCATGGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTAt                                                                                                                                       <  2:264078/294‑1 (MQ=255)                                                                                                                                                              aCGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGACGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCAACGCG‑CCGGCCGGCAACCACCCGGTTTATATCCGTTATACTGGGATTGGTacaggt                                                                                                                                   >  2:449981/1‑290 (MQ=255)                                                                                                                                                                gcgAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCCCCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGa                                                                                                                                 >  1:48472/1‑295 (MQ=255)                                                                                                                                                                         tGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTTCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCC‑CCGGCCGGCAATCACACGGTTTATACCCGTGATAACGGGAATGGTATCGGTGGACCgcacgc                                                                                                                        >  2:563173/1‑290 (MQ=255)                                                                                                                                                                                   ctgCCTGACG‑TGCAAGCCGGCGGATCTCCCCTCCGCATGGGGGAAAATGCTGGATACGTAACAAAATCAAACCCGC‑GCCAATCTGACCCTCGACGTCCGTCGGCCAACCCTGGTACGGGATGCGCAAATGCAGGTGCATTTTTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGt                                                                                                              <  2:731218/293‑1 (MQ=255)                                                                                                                                                                                                     agGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAatat                                                                                             >  1:187277/1‑295 (MQ=255)                                                                                                                                                                                                      ggCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATc                                                                                            >  1:134386/1‑295 (MQ=255)                                                                                                                                                                                                      ggCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATc                                                                                            <  1:522216/295‑1 (MQ=255)                                                                                                                                                                                                          gATCCCCCCTCCGCTTTTAGGGAATCTCTGGTTACGTTCCAAAATCAACCCTGC‑GCCACACTGACCCTCGCCTGCCGTCTGCCAACCCTTGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTAGTCGCGAAGCGGTGCTGAATGCTATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATgc                                                                                        <  2:418841/295‑1 (MQ=255)                                                                                                                                                                                                                          tGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGGTTATATCAGTGATCACGGGTATGGTAACGGGTAAACGAAAGAACACGAAGGGCCGTGAGGGCTGAATAACAGTCGCGAACGCGAgggagt                                                                        >  2:22406/1‑291 (MQ=255)                                                                                                                                                                                                                            aGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACCCGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCCTTAGGGGCTGAATATAATGCCGGAACGAGGGGAACggg                                                                      >  2:689727/1‑294 (MQ=255)                                                                                                                                                                                                                             gggAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCt                                                                     <  2:134386/295‑1 (MQ=255)                                                                                                                                                                                                                             gggAAATGCGGGCTACTTTCCAAAACCCAACCCTC‑GCCAAACTGCCCCTCGACTGCCGTCTGCCACCCCTGGCACGGGATGCGCAATGGCAGGTGAATTGGGTGGAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGGCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCt                                                                     <  2:392287/295‑1 (MQ=255)                                                                                                                                                                                                                                    gCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTACCGGTGAACCGAAAGAACCCGAAGGCCATTCAGGTAGGACTATCATTCGAGAACCAGCGGAAAGGCCAGGTggg                                                              >  2:669918/1‑295 (MQ=255)                                                                                                                                                                                                                                              ttACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACtt                                                    >  1:494871/1‑295 (MQ=255)                                                                                                                                                                                                                                                     aTCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTcaca                                             <  1:193554/295‑1 (MQ=255)                                                                                                                                                                                                                                                       cAAACCAGC‑GCCAAACGGACCCTCGACTGCCGTCTGCCAACCCGGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAAc                                           <  1:271937/295‑1 (MQ=255)                                                                                                                                                                                                                                                         aaCCAGCGGCCAAACTATCCCTCGAGGGCCTGCGGCCAACCCGGTGACTGGATGGGCAAAGGCAGTGGCATGTGTTGCAAATTAGTGGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAAcc                                          <  2:683048/295‑1 (MQ=255)                                                                                                                                                                                                                                                          aCCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCtt                                        >  1:428779/1‑295 (MQ=255)                                                                                                                                                                                                                                                           ccAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCCACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGGTTGGGAGCGGTGAACCGAAAGAACCCCACGGGCATTATGGTCTGAATATCATGCCCGAACGCACGGAACCGATAGGGGGGGAGCCGACCTTTTAACaaacata                                       >  2:415695/1‑290 (MQ=255)                                                                                                                                                                                                                                                            cAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTcc                                      >  1:461009/1‑295 (MQ=255)                                                                                                                                                                                                                                                                    aaaCTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCcggcggcg                               >  1:731646/1‑295 (MQ=255)                                                                                                                                                                                                                                                                          aCCCTCGACGGCCGTCGTCCAACCCGGGGCATGGAAGGGCAAAATCAGGGGCATTTGTTGCAAATTATTGTCGAAGCGGTGCTGAATGCGATGAAGAACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGGTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGt                         <  2:505801/295‑1 (MQ=255)                                                                                                                                                                                                                                                                              tCGACTGCCTTCGTCCAACCCTGGCACTGGATGCGCAAATGCAGATGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGt                     <  1:399665/295‑1 (MQ=255)                                                                                                                                                                                                                                                                               caaCTGCGGTCTGACATACCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATGCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCGGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACACGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTg                    <  1:502904/293‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aCTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGACTATCATGCGCGAACGCGCGGAACGGCTAGGGGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGCCACGTTGGGGAg                  >  2:73259/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                            cgcaCCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCTATGAAGCACGCCAACGCAAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTc       <  2:494782/292‑1 (MQ=255)                                                                                                                                                                                                                                                                                               aCCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGct    >  1:42781/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                cccTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGctc   >  1:283471/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                 ccTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGctct  >  1:455564/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                   CCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGC‑AGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGC‑GCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCG‑CCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGCTCT  >  NZ_LN832404/2582997‑2583577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑