Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 2,741,904 G→A 84.8% H107Y (CAC→TAC)  rimM ← ribosome maturation factor RimM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324042,741,9040GA84.8% 151.8 / 15.6 59H107Y (CAC→TAC) rimMribosome maturation factor RimM
Reads supporting (aligned to +/- strand):  ref base G (7/2);  new base A (25/24);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGAAACAGGCTAATTATGCCAATCCACATAGCGCCGTCTTTTACCGTTTATCCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGA  >  NZ_LN832404/2741614‑2742187
                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                           
tggAACCAGGCTAATTATGCCAATCCACATAGCGCGTTCTTTTACCGTTTATCCGGTGGTTTAAAAACCAGGTTCCCAATCTACTTCGATTGAACGAGTATTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGtagtag                                                                                                                                                                                                                                                                                         <  2:330262/294‑1 (MQ=255)
           tAATTATGCCAATCCACATAGCGCCGTCTTTTACCGTTTATCCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTc                                                                                                                                                                                                                                                                              >  1:732542/1‑295 (MQ=255)
              ttATGCCAATCCACATAGCGCCGTCTTTTACCGTTTATCCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAg                                                                                                                                                                                                                                                                           <  1:291126/295‑1 (MQ=255)
                  gCCAATCCACATAGCGCCGTCTTTTACCGTTTATCCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGa                                                                                                                                                                                                                                                                       <  1:494187/295‑1 (MQ=255)
                    cAATCCACATAGCGCCGTCTTTTACCGTTTATCCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAgg                                                                                                                                                                                                                                                                     >  2:256929/1‑295 (MQ=255)
                          gcaTTGCGCTGTCTGTGTCCGTTTATCCGGTGGGTGAAAACACAGGATCCCAATCGACTTCGTTTGATCGAGGATTGAGTTCGACTTTCTTGTTAACCTGCCCATCGATGAACTGTACGAGACGTTACTTGATACCAACCGCATCTTTCAGGTGTGCTTTAATTACGAGAACGTCATTAGATCCTGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTg                                                                                                                                                                                                                                                               <  2:500312/294‑1 (MQ=255)
                           caTAGCGCCGTCTTTTACCGTTTATCCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGc                                                                                                                                                                                                                                                              >  1:327089/1‑295 (MQ=255)
                               gcgcCGTCTTTTACCGTTTATCCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATg                                                                                                                                                                                                                                                          >  1:205521/1‑295 (MQ=255)
                                cgcCGTCTTTTACCGTTTATCCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGa                                                                                                                                                                                                                                                         <  1:535579/295‑1 (MQ=255)
                                          tACCGTTTATCCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTACGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCCACCATGAATCCACcaca                                                                                                                                                                                                                                               >  2:703413/1‑295 (MQ=255)
                                              gTTTATCCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTACTGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCCTTGGTTACTACCTGGCAGCCCATCAGGTCTTACCAGTCGTAGTCCCCCTCTTCAAGCTGCGGCAGCTCCGATGAATCCACGACAAttt                                                                                                                                                                                                                                           >  2:511916/1‑295 (MQ=255)
                                               tttacacGGTGGTTTAAAAACCAGGATCCCAAGCTACTCCGATTGAACGAGTAGTGAGATTGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACATTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTc                                                                                                                                                                                                                                          <  2:344587/289‑1 (MQ=255)
                                                                aaaCCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACAGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATAAGGTCTTTCCAGTAGTAGTCCCCATCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTAACAATTCGTCAGCAGGt                                                                                                                                                                                                                         >  1:72999/1‑295 (MQ=255)
                                                                  aCCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCCGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTTGTCAGCAGGtta                                                                                                                                                                                                                       >  1:540079/1‑294 (MQ=255)
                                                                  aCCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTc                                                                                                                                                                                                                       <  1:374632/295‑1 (MQ=255)
                                                                          cccAATCTACTTCGATTGAACGAGTATTGATATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATc                                                                                                                                                                                                               <  1:638410/295‑1 (MQ=255)
                                                                                              cGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGACCGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCAATCAGGTCTTTCCAGTAGTAGTCCCCCCCTTCAAGCTGAGGCAGCTGCGATGAACCCACGACAATTTCCCACTTCGTCAGTCGGTTCCACGCCTCACGATCATCCAAGCCttccc                                                                                                                                                                                           >  2:583966/1‑292 (MQ=255)
                                                                                                      gagaTCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCCGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATAGTCAACGCCTTTCCGCTtgatg                                                                                                                                                                                   >  1:496451/1‑295 (MQ=255)
                                                                                                          tCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCCACGCCTTTCAGCTTGATGATCa                                                                                                                                                                               >  1:765906/1‑295 (MQ=255)
                                                                                                            gACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACCATTTCACCATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACCCCTTTCAGCTTGATGATCAtt                                                                                                                                                                             >  1:516184/1‑294 (MQ=255)
                                                                                                             aCTTTCTTGATAGCCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTATCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTGCCATCATATCGACGACTTTACCGAGATCGTAGCCATCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGACCCCCTCTTCAAGCAGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTAAACGCCTTTCAGCTTGATGATCATGt                                                                                                                                                                            <  1:297243/295‑1 (MQ=255)
                                                                                                               tttccgctaaaCTTGCCCATCTGGGCACGGTACTAGACGTTCCTCGATACCAAACGCTTCTTGCAGGTTTGCCTTAATGACGAGAATGTCATTAGATTCGTTTTCCATCATATCGCCGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTcc                                                                                                                                                                          <  2:273209/286‑1 (MQ=255)
                                                                                                                 tCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTg                                                                                                                                                                        >  1:412044/1‑295 (MQ=255)
                                                                                                                    tGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGAtt                                                                                                                                                                     <  1:142587/295‑1 (MQ=255)
                                                                                                                    tGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGAtt                                                                                                                                                                     <  1:98359/295‑1 (MQ=255)
                                                                                                                       tacccTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATtgtg                                                                                                                                                                  <  1:350659/292‑1 (MQ=255)
                                                                                                                                    gAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCttccagctt                                                                                                                                                     >  1:275520/1‑295 (MQ=255)
                                                                                                                                          cGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCCGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACCACAATTTCACAATTAGTCAGCAGGTTCGACGAATCACGATCGTAAACGCCTTTCAGCTTGATGAACATGTCATGATTGTGGTACttcccgctttcatgc                                                                                                                                               >  2:235133/1‑291 (MQ=255)
                                                                                                                                           ggTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCCAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACCATTCGTCAGCAGGTTCCCCCCATCCCTATCGTCAACCACTTTCCACTTGATGACCATGTCCTGATTGTGGTTCctactgctttccagct                                                                                                                                              >  2:148311/1‑295 (MQ=255)
                                                                                                                                            gTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCGGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTg                                                                                                                                             <  1:183437/295‑1 (MQ=255)
                                                                                                                                                gagaCGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGGGGTGCTTCAAGCTTTCCAGCTGGACt                                                                                                                                         >  1:457880/1‑295 (MQ=255)
                                                                                                                                                    cGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTGCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTtgct                                                                                                                                     <  1:703413/295‑1 (MQ=255)
                                                                                                                                                          ttGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGCATCCACGACACTTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCCGCTTGCTGATCCTGTCCTTATTCTCGTGCTTCCAGCTTTCCAGCCTGACTTGCTGCCaat                                                                                                                               >  2:692982/1‑293 (MQ=255)
                                                                                                                                                             aTACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCGGGCCTTGCTGCCACTgcc                                                                                                                            >  2:120360/1‑293 (MQ=255)
                                                                                                                                                              tACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTGGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGAcc                                                                                                                           <  1:235133/295‑1 (MQ=255)
                                                                                                                                                                      cgcttttttgatgttGCCGTAGTGGCGGGAAATTCATTATATCTGTTTTCTAGCATACCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCt                                                                                                                   <  2:298926/282‑1 (MQ=255)
                                                                                                                                                                      agacatgtaagtgttGCCTGAATGACGGGAATGACATTAGATCCTGTTTGCAGTAGATCGACGGCTTTACCGAGAACGTAGCCTTCAGTGGGTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCt                                                                                                                   <  2:228682/282‑1 (MQ=255)
                                                                                                                                                                            ttCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATaa                                                                                                             <  1:351307/295‑1 (MQ=255)
                                                                                                                                                                              cAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAAc                                                                                                           >  1:652427/1‑295 (MQ=255)
                                                                                                                                                                                       cTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGa                                                                                                  >  1:167808/1‑295 (MQ=255)
                                                                                                                                                                                          aaTGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGTTTCTTCCAGCTTTCCACATGGACTTGTTGCCACTGACCCGCCTTCTGGATAAACCCGGGCTGAtcg                                                                                               >  2:654624/1‑293 (MQ=255)
                                                                                                                                                                                           aTGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGt                                                                                              <  1:256929/295‑1 (MQ=255)
                                                                                                                                                                                            tGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTc                                                                                             <  1:425929/295‑1 (MQ=255)
                                                                                                                                                                                              aCGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCaa                                                                                           <  1:214018/295‑1 (MQ=255)
                                                                                                                                                                                                             gATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCgc                                                                            >  1:451429/1‑294 (MQ=255)
                                                                                                                                                                                                                       ccATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGATTGCGGCGTCCTGGGTGga                                                                  >  2:422333/1‑295 (MQ=255)
                                                                                                                                                                                                                         ttcgtattgtggacTTTACCTGGATGGTAGCCTTCAGGGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGgaag                                                                <  2:425057/284‑1 (MQ=255)
                                                                                                                                                                                                                          ttacatCCACGACTTTCCCGGTATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCGTTCCAGTAGTAGTCCCCCTCGTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGgaaga                                                               <  2:327089/291‑1 (MQ=255)
                                                                                                                                                                                                                           catATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCACGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCCCTGACCCGCCTTCTGGATAAACCAGGGCTGATCGTCAACAATGCTTTCGGCGTCTTCGGttgcagca                                                              >  1:551431/1‑288 (MQ=255)
                                                                                                                                                                                                                            atatCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATACACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGaaa                                                             >  1:772130/1‑295 (MQ=255)
                                                                                                                                                                                                                              atCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATCAACCAGGGCTGATAGACAAAAATGCTTTCGGCGTCTTCGGGGGAAGAAAac                                                           >  1:556044/1‑295 (MQ=255)
                                                                                                                                                                                                                              atCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAac                                                           >  2:87942/1‑295 (MQ=255)
                                                                                                                                                                                                                              atCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAac                                                           >  1:227065/1‑295 (MQ=255)
                                                                                                                                                                                                                                         tACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGcca                                                >  1:565719/1‑295 (MQ=255)
                                                                                                                                                                                                                                              agaTCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGccacccac                                           >  1:152975/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                    ctacCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCAt                     <  2:652427/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tacCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCTACCCACGAATACCGTAAGACGAACCCAtt                    <  1:583966/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                      cccTGGCGGCCCTTCAGGTTGTTCCAGTATGAGTCCCCCGCTTCACGCGGTGGCAGCTGCGCTTGATCCACGACAATTGCACGATTCGTCAGCTGGTTCGCCGCATCACGATCGTCAACGCCTGTCAGCTTGATGATCATTTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCAttt                   <  2:496451/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                             aGCCCACCAGGTCTTCCCAGTCGTAGTCCCCCTCTGCAATCTGAGGCAGCTGCGATGCATCCACGCCAATTCCACAATTCTTCAGCAGGTTCTCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCaa            <  2:139875/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  aTCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGa       >  1:384452/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                   tCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGAt      >  2:549519/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                       gTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACAATCTGAGCAACCCACGAACACCGTAAGCCGAACCCATTTTTCCCAAAACGAAGGGa  >  2:352754/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                       gTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGa  <  1:654624/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                           
AGGAAACAGGCTAATTATGCCAATCCACATAGCGCCGTCTTTTACCGTTTATCCGGTGGTTTAAAAACCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCCACCCACGAATACCGTAAGACGAACCCATTTTTCCCAAAACGATGGGA  >  NZ_LN832404/2741614‑2742187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: