Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	3,106,724	C→A	90.7%	noncoding (56/76 nt)	EK12AG100_RS15430 →	tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	3,106,724	0	C	A	90.7%	238.7 / 11.1	75	noncoding (56/76 nt)	EK12AG100_RS15430	tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base A (31/37);  total (35/40)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

AGGCGGTTTATTGCTGCTGGCAACCGGATTACGCATCTGTGGCATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGTTTAACCATTTC  >  NZ_LN832404/3106434‑3107010                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                aGGCGGGTGTTTGCTGCTGTGAACCGGATTAGTGAGCTGTGGCATTAAAAGGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGAtt                                                                                                                                                                                                                                                                                             <  2:769197/295‑1 (MQ=38)   gCGGTTTTTGGCTGCGGGCAACCGGATTACGCAGCTGTGGCATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAAGGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTcc                                                                                                                                                                                                                                                                                           <  1:15774/295‑1 (MQ=255)   gCGGTTTATGTCGTCTGGCAACCGGATTACGCATCTGTGGCAGTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTcc                                                                                                                                                                                                                                                                                           <  2:286244/295‑1 (MQ=255)     ggttgttgtctgctgGAAACCGGATTACGCATCGGTGGCATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCCCTCCTTCTGGCAAGGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGa                                                                                                                                                                                                                                                                                         <  2:363260/286‑1 (MQ=255)      gTTTATTGCTGCTGGCAACCGGATTACGCATCTGTGGCATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATACCCGTGTCCTTGGTATGATTTCgtg                                                                                                                                                                                                                                                                                        >  2:546184/1‑293 (MQ=255)                gctgtgaaCCTGATTCTGCATCTGTGGCATTAAAAGTTTCCCGTTGGTCAACTGGCTTCCAGCACTCCTTCTGGCAAGGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTTACAATGCGTGCAATATCTGCAAAGTTAGTATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGcacc                                                                                                                                                                                                                                                                              <  2:734930/289‑1 (MQ=37)                   ggCAACCGGATTACGCATCTGTGGCATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCCGCCCGGTGCCCGGATAGCTCAGGCGGTAGGGCAGGGGGTTGAAAATACCCGGGGCCTTGGTTAGGTTGCGGATACGGGcacaaat                                                                                                                                                                                                                                                                           >  2:292235/1‑291 (MQ=255)                          ggATTACGCATCTGTGGCATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCtt                                                                                                                                                                                                                                                                    >  1:396005/1‑295 (MQ=255)                          ggATTACGCATCTGTGGCATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCtt                                                                                                                                                                                                                                                                    >  1:190255/1‑295 (MQ=255)                              tACGAATCTGTGGCATTAAAAGGTGCCCGGTGGTCAAAATGCGTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGa                                                                                                                                                                                                                                                                <  2:565393/295‑1 (MQ=255)                                cGCATCTGTGGCATTTAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCTCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAAc                                                                                                                                                                                                                                                              <  1:32158/295‑1 (MQ=255)                                  cATCTGTGGCATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAAccc                                                                                                                                                                                                                                                            <  1:12190/295‑1 (MQ=255)                                     ctggttccttAAAATTTGCCCGGTTGTCGACCTTCTTCCGTCACCTCTTCGGTCTATGCCGCTTTCCGCCGCCTGGTCCGCCTGTTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGcc                                                                                                                                                                                                                                                         <  2:438215/287‑1 (MQ=37)                                     cTGTTGCATTAAAATGTGCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCGGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGcc                                                                                                                                                                                                                                                         <  1:483800/295‑1 (MQ=255)                                      tgtgGCATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGccc                                                                                                                                                                                                                                                        <  1:79900/295‑1 (MQ=255)                                          gCATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACaa                                                                                                                                                                                                                                                    <  1:161996/295‑1 (MQ=255)                                           cATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAg                                                                                                                                                                                                                                                   <  1:38219/295‑1 (MQ=255)                                             ttAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGc                                                                                                                                                                                                                                                 <  1:319462/295‑1 (MQ=255)                                                aaaTGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCggg                                                                                                                                                                                                                                              <  1:326249/295‑1 (MQ=255)                                                       cccGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGc                                                                                                                                                                                                                                       <  1:74110/295‑1 (MQ=255)                                                         cGGTGGTCAACATGCTTCCCGCACTCCTTCTGGCAATGGCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGGAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCtt                                                                                                                                                                                                                                     <  2:267094/295‑1 (MQ=255)                                                         cGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTATGAACCCGCCCACAAGGCGGGTTTTTGCtt                                                                                                                                                                                                                                     <  1:581564/295‑1 (MQ=255)                                                               tCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGAt                                                                                                                                                                                                                               >  1:124953/1‑295 (MQ=255)                                                                cAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATc                                                                                                                                                                                                                              >  1:694754/1‑295 (MQ=255)                                                                   cATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGa                                                                                                                                                                                                                           >  1:36149/1‑295 (MQ=255)                                                                      gCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACaa                                                                                                                                                                                                                        >  1:625185/1‑295 (MQ=255)                                                                            aGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCt                                                                                                                                                                                                                  >  1:441376/1‑295 (MQ=255)                                                                               aCTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCCGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGACTTGACAATCACCttac                                                                                                                                                                                                               >  2:572319/1‑293 (MQ=255)                                                                                      cTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑aaaaa                                                                                                                                                                                                       <  1:721866/295‑1 (MQ=255)                                                                                              gCCTCTTGCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCt                                                                                                                                                                                               <  1:292235/295‑1 (MQ=255)                                                                                              gCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCt                                                                                                                                                                                               <  2:335850/295‑1 (MQ=255)                                                                                               ccGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAACAATTAGCtt                                                                                                                                                                                              >  1:745288/1‑295 (MQ=255)                                                                                               ccGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAACATTAGCtt                                                                                                                                                                                              >  1:612895/1‑295 (MQ=255)                                                                                               ccGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCtt                                                                                                                                                                                              <  1:96613/295‑1 (MQ=255)                                                                                                cGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACCATAACCTTCACG‑AAAAAACTTAGCTta                                                                                                                                                                                             >  1:308010/1‑295 (MQ=255)                                                                                                  cTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTtata                                                                                                                                                                                           <  1:171350/295‑1 (MQ=255)                                                                                                   tttCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATaa                                                                                                                                                                                          <  1:435730/295‑1 (MQ=255)                                                                                                   tttCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATaa                                                                                                                                                                                          <  1:706131/295‑1 (MQ=255)                                                                                                          cgccTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTtat                                                                                                                                                                                            <  2:307312/286‑1 (MQ=255)                                                                                                          cgccTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTtat                                                                                                                                                                                            >  1:307312/1‑286 (MQ=255)                                                                                                                           ttGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCa                                                                                                                                                                  <  2:190255/295‑1 (MQ=255)                                                                                                                           ttGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCACGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCAATAATGCTTAAGAACACGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCCCG‑AACAACATTCGCTCATAAAGTCTGGGGGAAGTACTCACGcaa                                                                                                                                                                  >  2:42851/1‑293 (MQ=38)                                                                                                                            tGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCAc                                                                                                                                                                 >  1:353652/1‑295 (MQ=255)                                                                                                                             tcgtGACTATGCGTGAAATATCGGCAATGATTGGGTAGATTGTTCAGTCTTCATGAAAGTGAAGACATTTTGTGGACGTGACGAGGCGAAGCAGGGTTAATGCGCCCCGTAGCCCGGTGAGCTCATTCGGTAGAGCACGTGAATGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGAGCACCACTAATGCTTAAGAACCCGCCCACAATGCTGGTTTTTGCTTTTGTATCTGACAATAACCTTCTCG‑TAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACg                                                                                                                                                                <  2:279212/292‑1 (MQ=255)                                                                                                                              ccTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGt                                                                                                                                                               >  1:476075/1‑295 (MQ=255)                                                                                                                                tGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTAATCTCGCAACGTTa                                                                                                                                                             >  1:496457/1‑295 (MQ=255)                                                                                                                                      tGCGTGCAATAACGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACgaga                                                                                                                                                       >  2:275483/1‑295 (MQ=255)                                                                                                                                              atatCGGCAAATTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAAtttt                                                                                                                                               <  1:66690/295‑1 (MQ=255)                                                                                                                                              atatCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAAtttt                                                                                                                                               <  1:546184/295‑1 (MQ=255)                                                                                                                                                               atAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATCAAGTCTGGTGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCt                                                                                                                              >  2:112006/1‑295 (MQ=255)                                                                                                                                                               atAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCt                                                                                                                              <  1:275483/295‑1 (MQ=255)                                                                                                                                                                     tgtgCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACt                                                                                                                        <  1:720226/295‑1 (MQ=255)                                                                                                                                                                     tgtgCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACt                                                                                                                        >  2:153478/1‑295 (MQ=255)                                                                                                                                                                         cAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTAc                                                                                                                    >  1:225256/1‑295 (MQ=255)                                                                                                                                                                         cAGTCTGCAGTAAATTGAAGAAAGTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGGTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTAc                                                                                                                    <  2:677292/295‑1 (MQ=255)                                                                                                                                                                                     aaTTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCacac                                                                                                        >  1:700425/1‑295 (MQ=255)                                                                                                                                                                                     aaTTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAAc                                                                                                        <  1:223596/295‑1 (MQ=255)                                                                                                                                                                                           agaaATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGc                                                                                                  >  1:501520/1‑295 (MQ=255)                                                                                                                                                                                                 ttgattgaCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCACGTCCAAACCCCCACcgccgc                                                                                            >  2:488357/1‑295 (MQ=255)                                                                                                                                                                                                            gacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAACAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCCATCCCCAGCCGCTCCACCTTccacat                                                                                 >  2:133257/1‑290 (MQ=255)                                                                                                                                                                                                              cgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTTGCCACGTTAACGAGAGTAAATTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATcc                                                                               >  1:231542/1‑295 (MQ=255)                                                                                                                                                                                                               gagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATccc                                                                              >  1:91718/1‑295 (MQ=255)                                                                                                                                                                                                                   cGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGc                                                                          >  1:393492/1‑295 (MQ=255)                                                                                                                                                                                                                      agcAGGGTGAATGCGCCCCTGTGCCCGGATAGCTCAGGCGGTAGAGCAGGTGATTGAAAATCCCCGTGTCCTTGGTTAGATGCCGAGTCCGGGCACCACTAATTCTGAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTtc                                                                       <  2:359740/293‑1 (MQ=255)                                                                                                                                                                                                                         aGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGCTCTGACAATAACCTTCACG‑AAAAACATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTACTTTTATTGATATTAATCTCCTTATCCTTTACCCCCGTCCAAACCCCAGCCGCTGCATCTTCCCCCTCCCAGGCATCCca                                                                    >  2:388685/1‑295 (MQ=38)                                                                                                                                                                                                                                    gcCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATCTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGa                                                          >  1:245528/1‑295 (MQ=255)                                                                                                                                                                                                                                     ccccGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCCCCCTCCCAGGCTTCTCCACCGCCCTGAcc                                                        >  2:306737/1‑294 (MQ=255)                                                                                                                                                                                                                                          ttGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAAt                                                   <  1:769926/295‑1 (MQ=255)                                                                                                                                                                                                                                              ccGGGTAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATGCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTtgt                                               <  2:95547/295‑1 (MQ=255)                                                                                                                                                                                                                                                    aGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTCCCCCCGTCAAAACTCCAGCCGCTGCACATTCACCATCCCCGGCCTCTCAACAACCCTACCCTCAATTTGTGTCAcc                                         >  2:639205/1‑295 (MQ=39)                                                                                                                                                                                                                                                     gCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCAccc                                        >  1:409285/1‑295 (MQ=255)                                                                                                                                                                                                                                                        acggcgggtgagacggggATTGAAAATCCCGGTTTCCTTGGTTAGAGTCCGATGCCGTGCACCACTAATTCGTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTTGATCTGACAATAACCTTCGCG‑AAAAAAATTAGCTTATAAAGGCTGGGGGAATTACTCTCGCCACGTTAACGAGTGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCa                                     <  2:694754/281‑1 (MQ=255)                                                                                                                                                                                                                                                            cGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAgcgc                                 >  1:462871/1‑295 (MQ=255)                                                                                                                                                                                                                                                             ggTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCACACTCCAGCCGCTGAACATTCACCATCCCAGGCTTCCCAGCAGACCTGACATCAATTTGTTACACCCGCCGCGCa                                >  2:613756/1‑295 (MQ=255)                                                                                                                                                                                                                                                                    cAGGGGATTGAAAATCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATAttttt                         <  1:692273/295‑1 (MQ=255)                                                                                                                                                                                                                                                                         gATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATcc                    <  1:572319/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aTCCCCGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGtt            <  1:589339/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                   ccccGTGTCCTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGTTTa          >  1:255822/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                          tCCTTGGTTAGATGCCGGGTCCGGGCACCACGAATTCTTAAGAAACCGCCCACAAGGCGGGTTGTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGTTTAACCAttt   <  2:353652/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                           ccTTGGTTAGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGTTTAACCATTTc  <  2:625185/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                AGGCGGTTTATTGCTGCTGGCAACCGGATTACGCATCTGTGGCATTAAAATGTTCCCGGTGGTCAACATGCTTCCAGCACTCCTTCTGGCAATGCCGCTTTCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACG‑AAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCACGTTAACGAGAGTAATTTTATTGATATTAATCTCCTGATACTTTACCCCCGTCCAAACTCCAGCCGCTGCACATTCACCATCCCAGGCTTCTCAGCAGCACTGACATCAATTTGTGTCACCCGCAGCGCATATTTTTCATCCAGTGCGTTTAACCATTTC  >  NZ_LN832404/3106434‑3107010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑