Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	3,503,761	T→C	85.5%	N30D (AAC→GAC)	EK12AG100_RS17515 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	3,503,761	0	T	C	85.5%	207.9 / 25.7	83	N30D (AAC→GAC)	EK12AG100_RS17515	membrane protein
Reads supporting (aligned to +/- strand):  ref base T (7/5);  new base C (38/33);  total (45/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

GCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCAC  >  NZ_LN832404/3503487‑3504050                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                   gcgGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCCGGACCGGGCGGAAGCCGTTATGGAAAACCGCCGCCGGGc                                                                                                                                                                                                                                                                                >  2:246500/1‑295 (MQ=255)       agcagcTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATgcg                                                                                                                                                                                                                                                                          <  1:404978/295‑1 (MQ=255)         cagcTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGcc                                                                                                                                                                                                                                                                        <  1:252471/295‑1 (MQ=255)                  tACAGGCAAAAGGCAAGTAAGGATCAACACGCCCCAGAAAGGGCAAAGTCCAAACGCCATAAGGCTGTTTATCGCCAGGAGGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGAt                                                                                                                                                                                                                                                               <  2:176609/295‑1 (MQ=255)                       gCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCAt                                                                                                                                                                                                                                                          <  1:159898/295‑1 (MQ=255)                           cAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATAccc                                                                                                                                                                                                                                                      >  1:35242/1‑295 (MQ=255)                                   tAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCa                                                                                                                                                                                                                                              <  1:601599/295‑1 (MQ=255)                                           aaCACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTTTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGTTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGccacca                                                                                                                                                                                                                                      <  1:718753/295‑1 (MQ=255)                                                   ccAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATc                                                                                                                                                                                                                              <  1:494120/295‑1 (MQ=255)                                                   ccAGATAGCGCAAAGACAAAACGCCATCAGGGGTGTCATCGCCAGGAAGCCGAGGATATCGGTGGGAAGAAAGAGTAACCAGGCGTTGAGCAGCCCGTTTTTCAGGGTAAAAGCGATACCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATc                                                                                                                                                                                                                              <  2:100235/295‑1 (MQ=255)                                                       aTAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTTCGGCCGGATCGGGCGGATGCCGTCATGACAAACAGGCACCGAACGATTCGCCAGGAGCCATGTCATCACCGTCGCGCCAGCCACCACGCTAATCTGac                                                                                                                                                                                                                          >  2:275994/1‑294 (MQ=255)                                                       aTAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGaa                                                                                                                                                                                                                          <  1:529802/295‑1 (MQ=255)                                                       aTAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGaa                                                                                                                                                                                                                          >  1:647639/1‑295 (MQ=255)                                                          gcgcCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAatat                                                                                                                                                                                                                       >  1:584018/1‑295 (MQ=255)                                                           cgcCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCCTGACAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCAAGCCACCACGATACTCCGAAtcta                                                                                                                                                                                                                      >  1:222435/1‑295 (MQ=255)                                                            gcCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACAACGATAATCTGAATATAc                                                                                                                                                                                                                     >  1:553468/1‑295 (MQ=255)                                                                aGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCATAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACTGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGAt                                                                                                                                                                                                                 <  1:398552/295‑1 (MQ=255)                                                                    cAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCAt                                                                                                                                                                                                             <  1:22938/295‑1 (MQ=255)                                                                      aaCGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGACTTCAATCAGTTGCGGCAGGAACGGGCGGATGCCGTCATGAAAAACAGACGCACCACGATGCGCCAGACGCGATGGCATACCCATCAGGCACGCCTCCGCGATATTCTGCATAACCAGATctcaag                                                                                                                                                                                                           >  2:74253/1‑290 (MQ=255)                                                                        cGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTc                                                                                                                                                                                                         <  1:37964/295‑1 (MQ=255)                                                                          ccATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGt                                                                                                                                                                                                       >  1:667836/1‑295 (MQ=255)                                                                           cATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATACATAGTCGTg                                                                                                                                                                                                      >  1:624616/1‑295 (MQ=255)                                                                            aTCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTTCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGAGGCGCCAGAAGCGATGTCATACCCGTCAGGCCCGCCACCACGATACACTCAATACACAGAACCaacatcctgc                                                                                                                                                                                                     >  2:610446/1‑286 (MQ=255)                                                                                 gCTGTTTAACGCAAGGAGGCCGAGGATAACGGTAGGAGGAAAAAGTACCCATGCGTTGAGCAGCCCGTGTTTCAGGGTAAACAAGATCCCAACCGAGGCCACAAAACCAATGCTCAGACCAAAAGAGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCtttt                                                                                                                                                                                                <  2:481223/295‑1 (MQ=255)                                                                                      ttATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTc                                                                                                                                                                                           <  1:96381/295‑1 (MQ=255)                                                                                      ttATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTGTTATTTc                                                                                                                                                                                           <  1:450595/295‑1 (MQ=255)                                                                                      ttATCACCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATTCATCGTCGGGCCTTTTATTTc                                                                                                                                                                                           >  1:725776/1‑295 (MQ=255)                                                                                       tATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGACTTCAATCAGTTGCGGCAGGATACGGCGGATGCCGTTCTGAACAACAGCCGCCGCGCGATGCGCCAGAAGCGATGTCATACCCGTCAAGCACGCCACCCAGACACTCTCAAAATCCACACACATAGACTTGCCTTTTATTTCa                                                                                                                                                                                          >  2:467117/1‑295 (MQ=255)                                                                                         tcgaccggACGCCGGGGATATCGGTAGGAAAAAAGAGGAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGa                                                                                                                                                                                        <  2:35242/289‑1 (MQ=255)                                                                                           gCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATg                                                                                                                                                                                      >  1:166647/1‑295 (MQ=255)                                                                                                      gAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCa                                                                                                                                                                           >  1:195878/1‑295 (MQ=255)                                                                                                       aGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATACTCTGAATATACAGATCAATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCAt                                                                                                                                                                          >  1:62835/1‑295 (MQ=255)                                                                                                            atCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCCTACCCGTCAGGCAAGCCACCACGGAAATCTGAATATCAAGATCACAAGTAGAGCCTTTTATTTCAAATGGTTAATCTGCATCGGaa                                                                                                                                                                     >  2:290293/1‑295 (MQ=255)                                                                                                                gTAGGAATAAAGATTAACCATTCCGTGAGCATACAGGTTTACTGAGTAAACAAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTGGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATcgc                                                                                                                                                                 <  2:559424/295‑1 (MQ=255)                                                                                                                gTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCCGAAGCGATGTCATACCCGTCCGACACGCCACCACGATAATCCGAATATCCAGATCCATAGTAGTGCCTTTTATTTCCGATGGGTAATCAGTCTGGGAATtgc                                                                                                                                                                 >  2:528471/1‑295 (MQ=255)                                                                                                                gTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAACAACAGCCGCCGAGCGATGCGCCCGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCCGAATAAACAGCTCCAACGTCGTTCATTTTATTTCAGATGGTTAAACCCCACAGGAATcgc                                                                                                                                                                 >  2:467352/1‑295 (MQ=255)                                                                                                                    gaagaaAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAGAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCgtgt                                                                                                                                                             <  1:246500/295‑1 (MQ=255)                                                                                                                      agaaagaGTAAACATGCGTTGAGCAGCCCGGTGTTCAGGGAAAAAGAGATCCCCACCGAGGCCACAAAACAAAGGCTAAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTGCAATCAGGTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTc                                                                                                                                                           <  2:602427/295‑1 (MQ=255)                                                                                                                              tAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGAAGTGCt                                                                                                                                                   >  1:171459/1‑295 (MQ=255)                                                                                                                                aCCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCCCCACGCACATCTGTATACAAAGACCCACAGTCGTTCCTTTTCTTACAGATGGTTCATACGCATACGAATCGCGTGTTCGCGGTGCGCa                                                                                                                                                 >  2:16021/1‑295 (MQ=255)                                                                                                                                   aTGCGTTGAGCAGCCCGGGTGTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAAGGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACg                                                                                                                                              <  1:461542/295‑1 (MQ=255)                                                                                                                                      gttttagcagcCCGTTTTTAAGAGTAAACAAGGTCCCCACCGACACCACAAAACCCATTCTAAGACCAAAAGCGAGCCTCCCCGCCTCGCGCCGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGaa                                                                                                                                           <  2:166647/290‑1 (MQ=255)                                                                                                                                         tGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAgg                                                                                                                                        >  1:66830/1‑295 (MQ=255)                                                                                                                                          gAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCAACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGc                                                                                                                                       <  1:486630/295‑1 (MQ=255)                                                                                                                                                  cGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCAACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCCGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑cc                                                                                                                              >  1:154116/1‑295 (MQ=255)                                                                                                                                                       ttCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCCGAAGCGATGTCATACCCGTAAGGCACGCCAACACGATAATCTGACTATCCAGATACATAGTCGTGCCTTTTATTTCAGATGGTTACTCAGCATCGGCATAGCGTGTTCGCACAGCTCAACGGAAAGGCCACACG‑CCACttt                                                                                                                         >  2:542998/1‑295 (MQ=255)                                                                                                                                                         cAGGGTAAAAGAGCTCCCCACCGACGCCACAAAACCAATGCGCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTc                                                                                                                       <  1:355210/295‑1 (MQ=255)                                                                                                                                                                   acgaaccccACACACGCCACAAAACCAACGCCCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCCTATAGCCTTAAAGCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGAt                                                                                                             <  2:370450/290‑1 (MQ=255)                                                                                                                                                                            aCCGGGGCCACCAAACCAAGGCGAGGACCAAAAGCGAGACTCCCCGCCGCGGGACGGTTGATATAGCCTTAAAGCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGAGGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGc                                                                                                    <  2:88921/295‑1 (MQ=255)                                                                                                                                                                            aCCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGAACCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTTCTCAACGGACAGGCCCAACC‑CCAATTTTCCTTCCGCGATCCTTTTGGc                                                                                                    >  2:446634/1‑295 (MQ=255)                                                                                                                                                                               gAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAAGCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGat                                                                                                 <  2:381820/295‑1 (MQ=255)                                                                                                                                                                                    cacaAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCCCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCCATCATTTTCGCCGTATGcc                                                                                            >  2:344876/1‑294 (MQ=255)                                                                                                                                                                                         aaCCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCCGACGCGATGTCATACCCGTCAGGCACGCCACCACGCTACTCTGAATATACAGAACCATAGTCGTTCCTTTTATTTCAGATGGTTAATCATCATCGGAATCTCTTGTTCGACGTGCTCAACGGAAAGGCCAACAC‑CCACTTCTCCTTCAGGCAACCTTTTGGTGaattcaccgttt                                                                                       >  2:563979/1‑284 (MQ=255)                                                                                                                                                                                          aCCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCCGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAAGCAGCCTCCGCATCGAGTGTTCGACGTGCTCAACGGCAAGGCACAACGACCAC‑TTTCCTTCATCTATCATTTTGGAGATATGTGCGTCtt                                                                                      >  2:198715/1‑295 (MQ=255)                                                                                                                                                                                            cAATGCTCAGACCAAACGCTCTACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTc                                                                                    <  1:42377/295‑1 (MQ=255)                                                                                                                                                                                            cAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCCGGCACGCCACCACGATAATCTGAATATACAGAACCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAAACGCGTGTTCGACGTGCTCAACGGCACGGCCAAACG‑CCACTTTTCCTTCAACGAGCATTTTGGAGTTATGCGCGTGTTTc                                                                                    >  2:555262/1‑295 (MQ=255)                                                                                                                                                                                             aaTGCCCAGACCAAAAGCGATACGCCCCGCCTCGGGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGAGCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCg                                                                                   <  2:66830/295‑1 (MQ=255)                                                                                                                                                                                              aTGCTCAGACCAAACGCGAGCCCCCCGCCCTCGCGACGGTTCATATAGCCTTCAATCAGTGGCGGCCGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGc                                                                                  <  2:34545/295‑1 (MQ=255)                                                                                                                                                                                                 cccAGACCAAACGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCttt                                                                               <  1:131235/293‑1 (MQ=255)                                                                                                                                                                                                  tCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGAACATTTTGGCTATATGCTCGTTTTTCGCTTTa                                                                              >  2:284342/1‑295 (MQ=255)                                                                                                                                                                                                    aGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAAt                                                                            >  1:155022/1‑295 (MQ=255)                                                                                                                                                                                                    aGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAAATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGCAAGGCCAACCG‑CCACCTTTCATTCACCGCTCATTTTTGTGACACGACCGTCTTTAGCttttct                                                                            >  2:544517/1‑292 (MQ=255)                                                                                                                                                                                                       ccAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGcc                                                                         >  2:623250/1‑295 (MQ=255)                                                                                                                                                                                                        cAAAAGCGAAACCCCCGGCCCCGCGACGTGTCATATAGCCGTCAATCAGTTGGGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCt                                                                        <  2:584018/295‑1 (MQ=255)                                                                                                                                                                                                          aaaGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTTCTCAACGGAACGGCCAAACG‑ACCCTTTTCCTTCAGCGATCATTTTGGGGAAATGCCCGTCTTTCGCTTTAATTCCttg                                                                      >  2:700234/1‑293 (MQ=255)                                                                                                                                                                                                              cGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTCATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCCCCTTTCCTTCCGCCCTCATTCTGGCCATTCTCCCGTCCTTCCCCTTAATGCTTTGtttt                                                                  >  2:353215/1‑294 (MQ=255)                                                                                                                                                                                                                     ccccGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATgg                                                           <  1:678269/295‑1 (MQ=255)                                                                                                                                                                                                                        cccctctagACGGTTCATATATCCTTCAATCAGTTGCGGCAGGATCGTGCGGATGCCATCAAGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTAGTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGc                                                        <  2:597978/287‑1 (MQ=255)                                                                                                                                                                                                                                gACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCTATGCTCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTg                                                <  1:164530/295‑1 (MQ=255)                                                                                                                                                                                                                                 aCGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGt                                               >  1:598054/1‑295 (MQ=255)                                                                                                                                                                                                                                  cGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGACAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATCATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGCTGGTTAATCAGCATCGGAATCGCGTGTTCGACCTGCTCAACGGACAGGCCAAACG‑CCCTTTTTCCTTCCGCGCTAATTTTGGCCCACAGCTAGTACTTCGCTTTAATGCCTGGTTTGGCAAGGGTTCCACttgcgtt                                              >  2:543645/1‑290 (MQ=255)                                                                                                                                                                                                                                     ttCATATGGCCTTCAAGCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTtata                                           <  1:187968/295‑1 (MQ=255)                                                                                                                                                                                                                                      tCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAg                                          >  1:58593/1‑295 (MQ=255)                                                                                                                                                                                                                                      gcATATAGCCTCCAAGCATTTGCGGCAGGATCGGGCGGAGGCCGTCATGAAAACCAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAg                                          <  1:329841/294‑1 (MQ=255)                                                                                                                                                                                                                                                   aaTCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGa                             >  1:269168/1‑295 (MQ=255)                                                                                                                                                                                                                                                      cAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCCTCGGAATCGCGTGTTCGACGTGCCCAACGGAAAAGCCAACAG‑CCACTTTTCCTTTATCGCTCCTTTTCGCGATATCCTCGTCTTTCCCTTCAACGCCCGTCTTGCACACGTCGCACCTTTCGTTATCGCCTATGACagcactcg                          >  2:20420/1‑290 (MQ=255)                                                                                                                                                                                                                                                        gTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCa                        >  2:691581/1‑295 (MQ=255)                                                                                                                                                                                                                                                           gCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATc                     <  1:657491/295‑1 (MQ=255)                                                                                                                                                                                                                                                               cAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACa                 >  1:658184/1‑295 (MQ=255)                                                                                                                                                                                                                                                                  gATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATg              <  1:700234/295‑1 (MQ=255)                                                                                                                                                                                                                                                                      tggCTGATGACGTGAGGAAAAACAGCCGCGGAGCGAGGCGCCAGAAGCGATGTCATACCCGTCAGGCACGTCACCACGATAATCTGAATATACAGATCCATAGTAGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAAGCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCcgc          <  1:149244/294‑1 (MQ=255)                                                                                                                                                                                                                                                                      gggCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCcgc          >  1:262566/1‑295 (MQ=255)                                                                                                                                                                                                                                                                          ggATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGccc      >  1:138968/1‑295 (MQ=255)                                                                                                                                                                                                                                                                          ggATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAAGGGTGCAACTTTGGTTATAGCCTAACCCCGCGATGGCAACTCACAATGCcggcccc      >  2:697972/1‑290 (MQ=255)                                                                                                                                                                                                                                                                             tGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGACCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCTATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCCATCGACAATGCCGCGCCCGCa   >  2:489005/1‑295 (MQ=255)                                                                                                                                                                                                                                                                              gCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCAc  >  1:179985/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                   GCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACG‑CCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGCAATGGTGCAACTTTTGTTATAGCCTATCACCGCGATGGCAATCGACAATGCCGCGCCCGCAC  >  NZ_LN832404/3503487‑3504050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑