Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 3,799,456 T→G 72.9% K38N (AAA→AAC waaO ← lipopolysaccharide 3‑alpha‑galactosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324043,799,4560TG72.9% 111.2 / 37.2 59K38N (AAA→AACwaaOlipopolysaccharide 3‑alpha‑galactosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (7/9);  new base G (24/19);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 5.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

AAATGATATCTGCATCCAGATAAAGGACTTTAGGCGCCTTATTAATAAAGTAATCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCTGCTATATATTTATTTAGAAGAGTTACAAAACCAGCAATATCTCCCGGTTGATACAGATGG  >  NZ_LN832404/3799164‑3799746
                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                  
aaaTGATATCTGCATCCAGATAAAGGACTTTAGGCGCCTTATTAATAAAGTAATCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCCCCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTAATTTTTAATTTTAAAGCAAACAAAATTCACCAACCAAATAAAAAgtt                                                                                                                                                                                                                                                                                                  >  2:352591/1‑295 (MQ=255)
     atatCTGCATCCAGATAAAGGACTTTAGGCGCCTTATTAATAAAGTAATCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATAAGATATATTTAAATTCTGGTTTCATAATGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATAAGTAAAAATAAGGAAAAACTAACGAATTCCTTACATATATTTTAATATTGACACAATCAAAATTACGAAACAAAATAAAAAATTTTGGt                                                                                                                                                                                                                                                                                             >  2:457483/1‑295 (MQ=255)
                 aGATAAAGGACTTTAGTCTCCTTATTAATAAAGTAATCTGCAATAACAATACGAAAATATATTGCATGAGTCCAATTCTTATTGCTAGGCAATGAGCGCAGTCTATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGTAAACACAATCGACTTCCTTCATTATATTTTAATATTGAATCAATCTAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAg                                                                                                                                                                                                                                                                                 <  1:550780/295‑1 (MQ=255)
                   aTAAAGGACTTTAGGCGCCTTATTAATAAAGTAATCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCg                                                                                                                                                                                                                                                                               >  1:604336/1‑295 (MQ=255)
                    tAAAGGACTTTAGGCGCCTTATTAATAAAGTAATCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGa                                                                                                                                                                                                                                                                              <  2:461924/295‑1 (MQ=255)
                      aaGGACTTTAGGCGCCTTATTAATAAAGTAATCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATg                                                                                                                                                                                                                                                                            <  2:29031/295‑1 (MQ=255)
                               aGGCGCCTTATTAATAAAGTAATCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAAAAAGTTTTTGTCAGTTCCATAAGCGACTTCCAGACaa                                                                                                                                                                                                                                                                   >  2:567721/1‑295 (MQ=255)
                                  cgcCTTATTAATAAAGTAATCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGa                                                                                                                                                                                                                                                                >  1:606381/1‑295 (MQ=255)
                                       tattaATAAAGTAATCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTc                                                                                                                                                                                                                                                           <  2:51091/295‑1 (MQ=255)
                                          taataaAGAAAACTGAAATAAAAAAACAAAAATACTGTGCATAGTTCCAAATCTTGATGCTTGGCAATGAGCGCAGTCGATCACCATTAATCATATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTgc                                                                                                                                                                                                                                                        <  2:18476/295‑2 (MQ=255)
                                              aaaGTAATCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCt                                                                                                                                                                                                                                                    >  1:620810/1‑295 (MQ=255)
                                                aatAACCTTCAATAACAAAACGAAAATTTATTCACTGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCTATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTAc                                                                                                                                                                                                                                                  <  2:419866/293‑1 (MQ=255)
                                                  tAATCTGCAAAAAAAAAACGAAAATATATTGAAAGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCAATAATCAGATATATTTTAATTCTGGGTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACtt                                                                                                                                                                                                                                                <  2:443317/295‑1 (MQ=255)
                                                     tCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAACCGATGTCCAACCAAAGATTTTCTGTTTCTACTTTAt                                                                                                                                                                                                                                             >  2:445334/1‑295 (MQ=255)
                                                               cAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATc                                                                                                                                                                                                                                   <  1:140901/295‑1 (MQ=255)
                                                                     gAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAAc                                                                                                                                                                                                                             <  2:489859/295‑1 (MQ=255)
                                                                      aaaaTATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTGTACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACt                                                                                                                                                                                                                            <  1:246820/295‑1 (MQ=255)
                                                                           atatTGCATGAGTGCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAAtt                                                                                                                                                                                                                       <  2:37885/295‑1 (MQ=255)
                                                                               tGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGa                                                                                                                                                                                                                   >  1:63546/1‑295 (MQ=255)
                                                                                       tCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCACAACCAAATAGCAATTTTTTGACAGTTCAATAAGCAATGTCCAGACAAAAATTTTCTGTTTTTACTTTATGATCAAAATCAATACATAAATTAAAAAACTCaat                                                                                                                                                                                                           >  2:517104/1‑293 (MQ=255)
                                                                                                    gCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAac                                                                                                                                                                                              >  2:433334/1‑295 (MQ=255)
                                                                                                       aGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACAcc                                                                                                                                                                                           >  1:743207/1‑295 (MQ=255)
                                                                                                           aaTGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAACGATTTTCTGTTTCTACTTTATGATTATAATATATAACTGACTTAAGAAAATCAGTTTCCTGGACAAACCCcttct                                                                                                                                                                                       >  2:619469/1‑295 (MQ=255)
                                                                                                             tGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAACGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCtgctgc                                                                                                                                                                                     >  2:203922/1‑295 (MQ=255)
                                                                                                                     gTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTtc                                                                                                                                                                             >  1:267797/1‑295 (MQ=255)
                                                                                                                        gATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTtcctc                                                                                                                                                                          <  1:433334/295‑1 (MQ=255)
                                                                                                                                 tAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATa                                                                                                                                                                 >  1:428627/1‑295 (MQ=255)
                                                                                                                                       gATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAgca                                                                                                                                                           >  2:638974/1‑293 (MQ=255)
                                                                                                                                                 aaTTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCt                                                                                                                                                 >  2:531065/1‑295 (MQ=255)
                                                                                                                                                 aaTTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCt                                                                                                                                                 >  1:383278/1‑295 (MQ=255)
                                                                                                                                                            tataCTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCTGGAAATAGCAGAAATAATTACCTTATGTAATCTa                                                                                                                                      >  2:677414/1‑295 (MQ=255)
                                                                                                                                                             ataCTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTAt                                                                                                                                     >  1:117076/1‑295 (MQ=255)
                                                                                                                                                             ataCTGCAAAGCAAAGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTAt                                                                                                                                     <  2:606381/295‑1 (MQ=255)
                                                                                                                                                                      acccAAGGAATCAAAATACATACGATCAGCAACACCAAAAAAATCAGTAAAAATATGGAAACACCATCGACGTCCTGCATTATAGTTTAATATTGAAGCAATCGAAAGTCCGCAACCAAAGAGAAATTTTGTGTCAGTTCCATAAGCGATGTCCAGATAAAGAGTTTCTGTTTCTACTTTAGGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTAGTTCCTCCGGGAAAGAGCAGAAATAAGTACCTTATGTAATCTATCGTAATACt                                                                                                                            <  2:514674/293‑1 (MQ=255)
                                                                                                                                                                       gCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACtt                                                                                                                           <  1:671667/295‑1 (MQ=255)
                                                                                                                                                                       gCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACtt                                                                                                                           >  1:322157/1‑295 (MQ=255)
                                                                                                                                                                         cAGGGCATCAAAATACTTACGATCCTCATCACCAACATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCCACAAAATAGCAAGTTTTTGTCAGTTCCATACGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCATTAACTGAATTAAGACACTCAGTTTCCTGGCAAACCTCCTACTGCATTATTTACTACGGTACATATCATAAATACTTACATTATGTAATCTactgtacacctttg                                                                                                                         >  2:202182/1‑282 (MQ=255)
                                                                                                                                                                         cAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAACTAGCAGAACTAATTACCTTATGTAATCTATCTTAATACTTCg                                                                                                                         >  2:94144/1‑295 (MQ=255)
                                                                                                                                                                             gCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTg                                                                                                                     <  2:620810/295‑1 (MQ=255)
                                                                                                                                                                             gCATCAAAATACTTACGATCATACTCACCCAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTGTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTg                                                                                                                     <  2:327385/295‑1 (MQ=255)
                                                                                                                                                                                      tACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATTGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTAGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATTGTAATACTTCGATTGGTAAAATTc                                                                                                            <  1:242746/295‑1 (MQ=255)
                                                                                                                                                                                             gATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCatcgatc                                                                                                     >  1:46994/1‑295 (MQ=255)
                                                                                                                                                                                                  tcatcaCCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCTATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGc                                                                                                >  1:660522/1‑295 (MQ=255)
                                                                                                                                                                                                    atcaCCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTCCTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTCTTTCCTCCGGGAACTAGCAGAATACATTACCTTATGTATCCTATCTTAATATTTAGATTTGTACAATTCATCTATATACGCac                                                                                              >  2:624738/1‑294 (MQ=255)
                                                                                                                                                                                                             ataatCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAAtttttt                                                                                     >  1:558352/1‑295 (MQ=255)
                                                                                                                                                                                                                  cAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGt                                                                                >  1:317791/1‑295 (MQ=255)
                                                                                                                                                                                                                   aGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGtt                                                                               <  1:722468/295‑1 (MQ=255)
                                                                                                                                                                                                                       aaaaTATGGAAACACAATCGACTTCCTTCAGTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGACACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCTGGTAAATAGCAGAAATAATATCCTTATGTAATTTATCGAATTACTTTGAGTGGTAAATTTTATCGTTCGATGTAGTAATTTTATAATGTTcttt                                                                           >  2:390676/1‑292 (MQ=255)
                                                                                                                                                                                                                           tatGGAAACACAATCGACTTCCTTCATTAAATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGa                                                                       <  2:343802/295‑1 (MQ=255)
                                                                                                                                                                                                                             tGGAAAAAAAAACGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAAGTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTGTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAAt                                                                     <  2:741542/295‑1 (MQ=255)
                                                                                                                                                                                                                               gAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAAttt                                                                   <  1:352591/295‑1 (MQ=255)
                                                                                                                                                                                                                                    acaATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCt                                                              <  1:213385/295‑1 (MQ=255)
                                                                                                                                                                                                                                       aTCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCTGCt                                                           <  1:531065/295‑1 (MQ=255)
                                                                                                                                                                                                                                          taCTTCCTTCATTAAATTTAAATAATGAAGCAAACGAAAATCCTCAAACAAATATAAAGTTGTTGTCAGTTCCATAAGCTATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCTGCtata                                                        <  2:555467/294‑1 (MQ=255)
                                                                                                                                                                                                                                                 ttaattttattttAAAATTGAAGCAACCGAAAATCCGCAACCAAATAGAAATTTTTTGTCAGTTCAATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCTGCTATAtatttat                                                 <  2:338459/292‑1 (MQ=255)
                                                                                                                                                                                                                                                             tAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTAATAGATCGACGCAGGAATTTTTTAATGTTCGATATGAATTTCAACTGCTATATATTTATTTAGCAGAGTTa                                     >  1:395420/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                       gCAATCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCTGCTATATATTTATTTAGAAGAGTTACAAAACCAGc                           <  1:445334/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aTCGAAATTCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCTGCTATATATTTATTTAGAAGAGTTACAAAACCAGCAat                        <  2:428627/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 ttCCGCAACCAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCTGCTATATATTTATTTAGAAGAGTTACAAAACCAGAAATATCTccc                 >  2:210071/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                         ccAAATAGAAAGTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCTGCTATATATTTATTTAGAAGAGTTACAAAACCAGCAATATCTCCCGGTTGATa         >  1:369984/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                              tAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTTGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCTGCTATATATTTATTTAGAAGAGTTACAAAACCAGCAATATCTCCCGGTTGATACAGAt    <  1:517104/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                gAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCTGCTATATATTTATTTAGAAGAGTTACAAAACCAGCAATATCTCCCGGTTGATACAGATgg  >  1:677368/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                  
AAATGATATCTGCATCCAGATAAAGGACTTTAGGCGCCTTATTAATAAAGTAATCTGCAATAACAAAACGAAAATATATTGCATGAGTCCAATTCTTAGTGCTAGGCAATGAGCGCAGTCGATCACCATTAATCAGATATATTTTAATTCTGGTTTTATACTGCAACGCCAGGGCATCAAAATACTTACGATCATCATCACCAAAATAATCAGTAAAAATATGGAAACACAATCGACTTCCTTCATTATATTTTAATATTGAAGCAATCGAAATTCCGCAACCAAATAGAAATTTTTTGTCAGTTCCATAAGCGATGTCCAGACAAAGATTTTCTGTTTCTACTTTATGATCATAATCAATAACTGAATTAAGAAACTCAGTTTCCTGGAAAAACACCTGCTGCATTATTTCCTCCGGGAAATAGCAGAAATAATTACCTTATGTAATCTATCGTAATACTTCGATTGGTAAAATTCATCGATCGACGCAGGAATTTTTTCATGTTCGATATGAATTTCACCTGCTATATATTTATTTAGAAGAGTTACAAAACCAGCAATATCTCCCGGTTGATACAGATGG  >  NZ_LN832404/3799164‑3799746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: