Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 4,023,922 T→G 7.1% intergenic (+221/+160) EK12AG100_RS19960 → / ← fadA NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324044,023,9220TG 7.1% 140.0 / 3.4 46intergenic (+221/+160)EK12AG100_RS19960/fadANAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA
Reads supporting (aligned to +/- strand):  ref base T (23/19);  new base G (2/2);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACC‑GGAACAACCC  >  NZ_LN832404/4023638‑4024211
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                    
gTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACattg                                                                                                                                                                                                                                                                                            <  1:610933/295‑4 (MQ=255)
                      cgGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATa                                                                                                                                                                                                                                                                    >  1:511555/1‑295 (MQ=255)
                            gATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCCTCTTATCGGGCCTACAAAATGT‑CC‑CAACCGTACGGCGGGTAAGGCgg                                                                                                                                                                                                                                                              >  2:177850/1‑294 (MQ=255)
                              tCGCCTTTTTGGCGATGGTTGTGCATTGATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACTGCAACTAAACTGTTATTCAGTGGCAGTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGttt                                                                                                                                                                                                                                                            <  1:405478/295‑1 (MQ=255)
                              tCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGttt                                                                                                                                                                                                                                                            <  1:123433/295‑1 (MQ=255)
                               ggCCGGTGTGGCGAGGCGTTGGCATGTACCTGCGATATAAAAAAACCCGCCCCGGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTa                                                                                                                                                                                                                                                           <  2:368229/294‑1 (MQ=255)
                               cGCCTGTTTGGCGATGCGTTTGCACTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTa                                                                                                                                                                                                                                                           >  1:631402/1‑295 (MQ=255)
                                gCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCCATTGGTGCATGATGCCTGATGCGACCCTGTCGCCTCTTTACGTGCCCACACATAGT‑CC‑GAACCGTAGGCCGGATAAGGCAttttc                                                                                                                                                                                                                                                          >  2:566626/1‑293 (MQ=255)
                                   tGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcgc                                                                                                                                                                                                                                                        >  1:719784/1‑295 (MQ=18)
                                           gATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATctg                                                                                                                                                                                                                                                >  1:125392/1‑293 (MQ=18)
                                            ttGCTTTTGCATTTATCTGAGAACTAAAAAACCCCGCCCCGGACAGGCGGTAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                                                                                                                              <  2:511555/294‑1 (MQ=255)
                                                 attGCTTTAATCTTATACATATAAAAACCCGCCCCGGGCAGGTGGGAAGAACTGCAACTAAACTGTTATGCTGTGGTGTTTGTAGCTATGACGTATAGGGCAAACCATGCCCGATGCGACGCGGGCGCGTCTGATCGTGCCTACAATGATTCCGAACCGTAGGCCGGATAATGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACTTg                                                                                                                                                                                                                                         <  2:718018/294‑1 (MQ=21)
                                                            cTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGc                                                                                                                                                                                                                              >  1:677313/1‑295 (MQ=255)
                                                                       aaaaaCCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACg                                                                                                                                                                                                                   <  1:637878/295‑1 (MQ=255)
                                                                                     gACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGCTGCGACGCTGGCGCGGCTTAt                                                                                                                                                                                                     >  1:424872/1‑295 (MQ=255)
                                                                                      aCAGGCGGGAAGAACGGCAACTAAACTGTGATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                                                                                                                    <  1:515053/295‑1 (MQ=255)
                                                                                                 gaaCGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTCCAAATAGT‑CC‑GAACCGTCGGCAGGGTAAGGCCTTTACGCCGCATCCGGCACTTGGTTCATGATTCCCGATCACAAGCTGCTGCGTATTCTCAGGTCtcaatt                                                                                                                                                                                         >  2:565571/1‑290 (MQ=21)
                                                                                                     ggCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACTTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTCGGCCGGATAAGGCGTTCACGCCGCATCCGGCCATGGGTGCATGATGCCTGCGGAGAAGCTGGCGCGTCTTAACAGGcccgcatctgtg                                                                                                                                                                                     >  2:272621/1‑285 (MQ=25)
                                                                                                     ggCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTAAGCCGCATCCGGCCATTTGGGAATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGACGACATTGATg                                                                                                                                                                                     >  1:415572/1‑295 (MQ=255)
                                                                                                            aaaCTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATACATTGGTGCCGGATc                                                                                                                                                                              >  1:335514/1‑295 (MQ=255)
                                                                                                             accgtgtgtccAGGGGCTTTTCGATCTATGACGGACCTGGCACACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCg                                                                                                                                                                             <  2:295343/285‑1 (MQ=255)
                                                                                                                      tCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCgg                                                                                                                                                                    >  1:767847/1‑295 (MQ=255)
                                                                                                                            gCATGTAGATGTATGACGTAGCGGGCAAAGCAGGGCCGGTGAGACGCTGGCGCGTCGTATCGGGCCTACAAATAGGCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAgg                                                                                                                                                              <  2:384313/295‑1 (MQ=25)
                                                                                                                             cATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATACGACGCTGTCGCGTCTTATCGTGCCTACCAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTCTCCGGCCCACCTTGGTGCCGGCTCGGTCTACCGGATCAGGc                                                                                                                                                             >  2:501301/1‑295 (MQ=25)
                                                                                                                                      cTATGTCGTATCTGGCAAACCATGCCCAATTCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcgcc                                                                                                                                                    <  1:515930/295‑1 (MQ=255)
                                                                                                                                         tGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCa                                                                                                                                                 <  1:177850/295‑1 (MQ=255)
                                                                                                                                                   ggCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGCAAAGGCGTTCACGCCCACTCCGGCAAGt                                                                                                                                       >  1:773849/1‑295 (MQ=255)
                                                                                                                                                      aatccATGCCCGATGCGACGCGGTCGCGGCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGt                                                                                                                                    <  2:79599/292‑1 (MQ=255)
                                                                                                                                                              cccGATGCGACGCTGGCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCg                                                                                                                            <  1:2141/295‑1 (MQ=255)
                                                                                                                                                                cgaggcgcggtggTCGGGTCTTATGGGGCCTAAAAATGGGCCGAACCGTAGGGCGGATAAGGCGTGTACGCGGCATCCGGCAATTGGTGCATGAGGCCTGATGCGACGCTGTCGCGTCTTATCGGGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCGGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCt                                                                                                                          <  2:441998/283‑1 (MQ=21)
                                                                                                                                                                cGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGCTAAGGAGTTCACGCCGCATCCGGCAAGTGGGTAAACCCGCt                                                                                                                          >  1:755372/1‑295 (MQ=255)
                                                                                                                                                                  aTGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCa                                                                                                                        >  1:362926/1‑295 (MQ=255)
                                                                                                                                                                  aTGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCa                                                                                                                        >  1:169090/1‑295 (MQ=255)
                                                                                                                                                                                   cTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAGTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATAcc                                                                                                       <  1:279271/295‑1 (MQ=255)
                                                                                                                                                                                          tGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCACGTGGTTAAACCCGCTCAAACCCCGTCGCAATAACCTGAcca                                                                                                >  1:605396/1‑294 (MQ=255)
                                                                                                                                                                                          tGCCTACAAAGAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTAGCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGAccc                                                                                                <  1:151339/295‑1 (MQ=255)
                                                                                                                                                                                                                    cgtgATAAGGCGTTTACGCCGCTTTCGGCAATTGGGGCATGATTCCTGATGCGACACTGTCGCGTCTTATCGTGCCTACAAATATT‑CC‑GAACCGTAGGCAGGATAAGGCGTTTAGGCCGCATCGGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGAcc                                                                      <  2:689831/292‑1 (MQ=37)
                                                                                                                                                                                                                             gCGTGTACGCCGCATCGGGCAATTGGTGCATGAGGCCTGATGGGACGCTGTAGCGTCTTATCGGGCCTACAAATAGT‑CC‑GAACCGGAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAAc                                                             <  2:568226/295‑1 (MQ=255)
                                                                                                                                                                                                                                       cgcATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATCCCCTGACCCAGACCGATACACATCGTCGCCAGACCAACCTGAACGTCTTTGCGt                                                   >  1:715982/1‑295 (MQ=255)
                                                                                                                                                                                                                                               gtatctggcaaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAg                                           >  1:240396/15‑295 (MQ=255)
                                                                                                                                                                                                                                                  aTTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGAtt                                        >  1:774609/1‑295 (MQ=255)
                                                                                                                                                                                                                                                     ggTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTcag                                     >  1:306647/1‑295 (MQ=255)
                                                                                                                                                                                                                                                             atgCCGGATGCGACTCTGTCGCGTCTTATCGTGCCTACAAATAGT‑AC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCgtgg                             <  1:565571/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                 cTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCAAGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCCGAACCAGACCGATACACCACGTAGCCCGACCACACCGAACGTCCTTTCGGTCCATAACATTCAGCAGCCTGGGGca                         >  2:755334/1‑294 (MQ=21)
                                                                                                                                                                                                                                                                        gACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GACCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATAcgc                  <  1:596607/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACgcgc                <  1:265025/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                           gCTTTCGCTTCTGTTTGTTCCTACCAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGACTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTAGACCAACCAAACTGAACGTCTTTGCGTTCCATCAGATTACACCGCGTGGTTCTGATCcgccac               >  1:251501/1‑292 (MQ=25)
                                                                                                                                                                                                                                                                                  cgTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACC‑GGaac       >  1:294031/1‑295 (MQ=255)
                                                                                                                                                                                                                                                                                     ttgttCGTGCCTACAATCAGT‑CG‑GAGACGTGGGCATGTGAAGGCGTGGACGACGCCTCCTGCAATTGGTGCAGGATGCCTGATGCGCCGGTTGGGCGTCGTATCAGACCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCAGCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACC‑GGaacaac    <  2:755206/291‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        aTCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCCAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGCCCGATACACATCGCCGCCAGACCAAACTGCAACTCTTTGCGTTCCATCAGCTTCAGCCGCGGAGACCTGATACGCGCCCCGGGAACAAccc  >  2:532165/1‑295 (MQ=25)
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                    
GTTTTGCAGTGAGCGTAATGCGCGGGAAGATCGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACC‑GGAACAACCC  >  NZ_LN832404/4023638‑4024211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑