| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NZ_LN832404 | 4,190,469 | G→T | 86.1% | A70D (GCT→GAT) | thiE ← | thiamine phosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_LN832404 | 4,190,469 | 0 | G | T | 86.1% | 149.5 / 15.7 | 58 | A70D (GCT→GAT) | thiE | thiamine phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base G (2/6); new base T (24/26); total (26/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01 | |||||||||||
CGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATA > NZ_LN832404/4190189‑4190758 | cccagcggggtgcgaaTCCGTGAGTCGCTAGAGCTGCTGTGCCAGCTGTGACAGCCGCTGCGGAGCGGAATTGATCTGGTTGGTTTGCGTCGGGAACACATGTTCATGCGAGATATAAGGGGGGCGTGCTGCCAGCGCGACGTCGATGTCCAGAGCGTCATGTGTCGAACAGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑At < 2:28320/281‑1 (MQ=255) aCGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATATTCCTGCCCCAAATGGACGCCATACACCTGATGCTTGATCGCCAGCCGCCACTAACCGTTGATCACCA‑ATcgcc > 2:757059/1‑294 (MQ=255) cGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATcgcgc > 1:356915/1‑295 (MQ=255) ggTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGCCGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATcgcgcg > 1:94213/1‑295 (MQ=255) tGGGATAATCCGCCCGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGtt < 1:136059/295‑1 (MQ=255) ggATAATCCGCCAGTCGCGCAACATGCCGTGCCAGCTGTTCCAGCCCCGGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTtat < 1:468891/295‑1 (MQ=255) caccgggCCGGGCCAGCGGGCCCACCCCGTGGGGGTGAGAAGGCATTGGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGAGATAAGAGGGGCGTGCGGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAAt < 2:32593/289‑1 (MQ=255) tGCCGTGCCAGCTTTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTgccg < 1:757059/295‑1 (MQ=255) gTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAAATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGCTAGCCCGACGCCAGTAAGCGTTGATAAACA‑ATCCCGCGTTATACCGGCCGCCCACCCCACTTgccagccc > 2:555143/1‑290 (MQ=255) cAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCAAGTCATCGTTGATAAACA‑ATCGCGCGTTATAGCGTCGGCCACGCGCAATTTCCGCCAcacccaatgattca > 1:474107/1‑282 (MQ=255) gTGCAGAAGGCAGTTGTTTGGTTTGCGTCGGTAACACATGTCCCAGCGCGATAGAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATc < 1:671056/295‑1 (MQ=255) gCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATcgc > 1:634034/1‑295 (MQ=255) aGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCgcgc > 1:195706/1‑295 (MQ=255) gAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGAGGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCCGTAATCGTTGATAAACA‑CTCGCGCCTTATCCACGCGGCCCAGCCACATTGCCGCCCAGACATCCGCTTCCACCTCTTCATCCCGcc > 2:199572/1‑295 (MQ=255) ggCATCGGTGTGGTTTGCGTCGGGAACACAGGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGAt < 1:227339/295‑1 (MQ=255) ggAACACAGGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATGTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTAAAGCGAGCCGAGCTTTGATGCGTAGCTGGAGAgt < 1:120341/295‑1 (MQ=255) aGCGCGATATAAGAGGGGCGGGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCAt < 1:687489/295‑1 (MQ=255) cgcgGTATAAGAGGGGCGGCGTGCCGGCGCGACGGTGATTTCCATTTCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCCGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATcc < 2:88235/295‑1 (MQ=255) cggaggagggggggCGGGCTGCCAGCGCGACGGCGCTTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGGGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCaa < 2:226009/285‑1 (MQ=255) taAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCAGGTGTCGAACCGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGAc < 1:397148/295‑1 (MQ=255) gaggggggCGGGCTGCATGTGCGACGTCGATTTCCGTATCGTCACGGGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGTATGGCATTGAGATCGGTGGCGTGCAAATCTTCCGGCCCCAAATGGAGGCCATACGCCTGAGGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACg < 2:224475/291‑1 (MQ=255) ggCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGAt < 1:329585/295‑1 (MQ=255) cgccgtctcctcgctACGTCGATTACAAGATCGTCAGGTGGCGAACCGCCCGGCCGCAGGACTGCCGCGCGGATGGCATTGAGTTCGGTGGCGTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATcc < 2:94213/284‑1 (MQ=255) tgctgcCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCAc < 1:126369/295‑1 (MQ=255) gctgcCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAACCGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACTGACGTTCGATCCACt < 1:273855/295‑1 (MQ=255) aGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGCGATGCAGTACGCCTGCATCCACCACACGTTCGCGCCACTGtcccc > 2:161769/1‑291 (MQ=255) aGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGc > 1:403561/1‑295 (MQ=255) cGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑ccac > 1:51848/1‑295 (MQ=255) gTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑ccacc > 1:654048/1‑295 (MQ=255) tcttCATGTGTCCAACCCCCCGGCCGCAGGCCGCCCGCGCGGCGGGCATTGGTATCGTTGGCTTGCAAATCGTCCTTCCCCCAATGGACGCCATACGCCTGATCCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACC‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTc < 2:537487/292‑1 (MQ=255) cGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGCTCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCCTCCAACAGACGTTCGATCCCCTGGACTCTTT‑CCCACCCCGGGTACAGTcc > 2:275076/1‑295 (MQ=255) gTCATGTGTCTAACCCCCCAGCCGAAGTCCTGCCGCGCGGAGGTCTGTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGACCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCt < 2:195706/295‑1 (MQ=255) gtggCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACg < 1:613109/295‑1 (MQ=255) cAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTTATGCGTAGCTGCAGAGTACGTACGCCTGCATCCAACAGCCGTTCGACCCACCGTACCCCGG‑CCCACACCGGCTCCCACCCTGAACGAAAAggctcccga > 2:97323/1‑288 (MQ=255) ccGCAGGCCTGCCGGGCGGATGGCATGGGGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑ggagga < 2:388089/295‑1 (MQ=255) ccTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAg > 1:279731/1‑295 (MQ=255) tGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTAATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGc < 1:254668/295‑1 (MQ=255) cgcgcgGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGa < 1:277861/295‑1 (MQ=255) cgcgGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATa > 1:540736/1‑295 (MQ=255) ggATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGGACGTACGCCTGCATCCAACAGACGGTCGATCCCCTGTACGCTGT‑CCACCACCGGGTCCAGTCCCTCACGAAAAGGTACA‑AGCGGAAAATCAGGGTGAtcaca > 2:206041/1‑291 (MQ=255) tGGCAATGGGATCCGTGGCTTTCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CAc < 2:150957/295‑1 (MQ=255) aTCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGCGAGTACGTAAGCCTTCATCCCACAAACGTTCGATCCACTGTTAGCTGT‑CCAACACCGGGTACAGTCCTGACCGAAAA‑GCACACGGAGGAAAATCAGGCTGAACCAC‑CACGCTccccact > 2:329261/1‑291 (MQ=255) ggCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCCTCGCGCCGATCTTTGCTGCGTAGCTGGAGAGTACGTCCGCCTGCCTCCCCCAGACGTTCGATCCAATGTACGCTGT‑CCACCCCCCGGTCCAGTCCTGAACGACACGGTAAC‑GGAGCAAAATACAGCGGATACAA‑CACGCTTCCTAATCACGCa > 2:573614/1‑295 (MQ=255) cAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGCGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCCCCACCGGGTCCAGTCCTGAACGAAAAGGTACC‑GGAGGAAACTACGGCTGATACAT‑CAACCTTCCCCCTTACGCCGGTAgc > 2:58658/1‑294 (MQ=255) tCTTCCTGCCCCAAATGGACGCCATACGCCGGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTtc < 1:486823/295‑1 (MQ=255) ttCCGGCCCCAACTGGACGCCATACGCCTGAGGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCACAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTtct‑c < 2:634034/295‑1 (MQ=255) ccTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAACGGTACA‑GGAGGAAAATCAGGCAGATACATACAC‑CTTCCTCCTTACGCAGGTAGATTTCA‑ccc > 2:206109/1‑294 (MQ=255) cTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGc > 1:672359/1‑295 (MQ=255) tGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGcc > 2:79549/1‑295 (MQ=255) aTGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACCGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAA‑CACGCTTCCCCCTTACGCAGGTAGATTTCT‑CCGcccatgcccc > 2:89314/1‑287 (MQ=255) gACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTCCGCCTGCATCCAACAGAAGTTCGATCCACTGTACGCTGTCCCACCA‑CGGGTACAGTCCTGAACGACAGGGTACA‑GGAGGTACATCAGGCGGATCCAC‑ACCGCTTCCTCCCTACGCAGGTGGTTTTCTACCGccatgacccgca > 2:254679/1‑285 (MQ=255) tACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCCTCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCACCAGACGTTCCATCAACTGTACGACGT‑CCCACACCGGGTACAGCCCTGACCGACAAGGTACA‑GGAGGAAAATCAGGACGATACAT‑CAAGCTTCATCCTTACGCAAGTCGAATTAT‑ACGCCACTGGCTCgtacataact > 2:144606/1‑286 (MQ=255) aCGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTtctcg > 1:270981/1‑294 (MQ=255) ttGATCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGc > 1:234719/1‑295 (MQ=255) gctCGCCAGCCGCCCGTAAGCGTTGAGAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCa < 2:51848/293‑1 (MQ=255) tCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCAtt < 1:275076/295‑1 (MQ=255) tCGCCAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCAtt < 1:330783/295‑1 (MQ=255) tCGCCAGCCGCCAGTAAGCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCAtt < 1:135391/295‑1 (MQ=255) ccAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTccc < 1:252722/295‑1 (MQ=255) cAGCCGCCAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCa < 1:13042/295‑1 (MQ=255) gccgccAGTAATCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCAtt > 1:202165/1‑295 (MQ=255) gccgccAGTAAGCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCAtt < 1:523119/295‑1 (MQ=255) cgccAGTAATCGTGGA‑AAACACATCGGGCGTGATAGCGGCGGCCCAGCCAAATGGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCGTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTc < 2:37299/295‑1 (MQ=255) aGTAATCGTGGATAAACA‑AGCGCGCGTTATAGCGCCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATa < 2:540736/295‑1 (MQ=255) | CGCCACGGTGGGATAATCCGCCAGTCGCTCAACATGCCGTGCCAGCTGTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACA‑ATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGT‑CCACCACCGGGTACAGTCCTGAACGAAAAGGTACA‑GGAGGAAAATCAGGCTGATACAT‑CACGCTTCCTCCTTACGCAGGTAGATTTCT‑CCGCCTCTGGCACGGAAGTTCTCCGACATATCCGCCATTCCCATTTCAATA > NZ_LN832404/4190189‑4190758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |