Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	4,212,143	T→G	79.9%	Y65D (TAT→GAT)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	4,212,143	0	T	G	79.9%	147.7 / 32.3	65	Y65D (TAT→GAT)	aceB	malate synthase A
Reads supporting (aligned to +/- strand):  ref base T (7/6);  new base G (24/28);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

TAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCC  >  NZ_LN832404/4211856‑4212423                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                          tAAATGAGTATTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCtgattttat                                                                                                                                                                                                                                                                                    <  1:738925/295‑1 (MQ=255)  aaaTGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCAAACCGCAATAAACTTCTGGCAGAGCGCATTCCGCAGCAGCCAGATATTGATCCCGGCACGTTTACtgattgtatt                                                                                                                                                                                                                                                                                   >  2:449372/1‑295 (MQ=255)     tGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCCAGATATTGATAACGGAACGTTGCCTGATTTTATTTCg                                                                                                                                                                                                                                                                                >  1:401459/1‑295 (MQ=255)      gAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCgg                                                                                                                                                                                                                                                                               >  1:25331/1‑295 (MQ=255)           gtCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGACGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGAGCATTCAGCAGCAGCACTATACTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACa                                                                                                                                                                                                                                                                          >  2:463188/1‑295 (MQ=255)              gtAGTTGGTCTAAACAAAAAGAGCACACCGTTCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGTAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCt                                                                                                                                                                                                                                                                       <  2:356652/294‑1 (MQ=255)               tAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCtt                                                                                                                                                                                                                                                                      >  1:610346/1‑295 (MQ=255)                attgatgCTAAACGAAAAGAGAACAACGATTCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGAACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTc                                                                                                                                                                                                                                                                     <  2:397941/290‑1 (MQ=255)                aGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGCGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGAAGGAGAAGCAAA‑TTATTACTGCCGAAGCGGTAGACTTTCTGACTGAGCTGGTGACGCATTTTACGCCACATCGCAATACACTTCTGGCAGCGCGCATTCAACAGCAGCTAGATCAGGATCAAGTAACGTAGCCTTATTTTCGTTAGGAAACaccatc                                                                                                                                                                                                                                                                     >  2:505483/1‑290 (MQ=255)                 gTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACACGGCCGTATGGCGAGCAGGAGAAGCAAA‑CTCTTACTGCCGAAGAGGTAGAATTGCTGACTGAGCTGGGCACGCAACTTACGCAACAGCGCAAGACACTTCTGGCAGCGCGACTTAAGCCGCAGCAAGCTATTGATACCGGACAGCTGCCTTATTCAATTTCGGaacctggtgtc                                                                                                                                                                                                                                                                    >  2:62811/1‑286 (MQ=255)                    gtgCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCACCAACGCAATAAACTTCTGGCAGCGCGAATTCAGCAGCAGCAAGCTAATGATAACGGAACGTTGCCTCACTTTATTTCCGAAACCACTTCCAtt                                                                                                                                                                                                                                                                 >  2:689969/1‑295 (MQ=255)                          aaCGAGAAGAGGACAAGCATCCTGCGGTGTAAGGGGGGAAGTGTTGGGGACACGGACGACGGGCTGTGCGATGACTGAACAGGCAACAACAACCTATGAACTGGCGTTCACAAGGCCGTATGGCGGGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCATAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGAt                                                                                                                                                                                                                                                           <  2:534189/295‑1 (MQ=255)                                        aaCGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAAt                                                                                                                                                                                                                                             >  1:200951/1‑295 (MQ=255)                                          cGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTc                                                                                                                                                                                                                                           <  1:137286/295‑1 (MQ=255)                                                     tCACAGTGGGGAAGGTTTCGGATCCATGACGAGTAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTTGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCAATGCTGATTGGAAAATTCGCGGGATTCCt                                                                                                                                                                                                                                <  1:578798/295‑1 (MQ=255)                                                         aGTGGGGAAGTTTTCGGATCCAGGACGAGGAGCTGAACGATGACGGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCgg                                                                                                                                                                                                                            <  2:58440/295‑1 (MQ=255)                                                                              aTGACGAGGTGCTGAACGATGACTGAAAAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTag                                                                                                                                                                                                       <  2:265294/295‑1 (MQ=255)                                                                                gACGGGGAGATGCACGATGACGAAACAGGCAACAACAACCGGTGAACTGGCTTTCACGAGGCCGTAGTGCGAGCAGGAGAAGCAAA‑GTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTagag                                                                                                                                                                                                     <  2:430373/295‑1 (MQ=255)                                                                                   gaggagCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATa                                                                                                                                                                                                  >  1:721167/1‑295 (MQ=255)                                                                                   gaggagCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCAGCGTAGAGATa                                                                                                                                                                                                  >  1:408795/1‑295 (MQ=255)                                                                                                    cTGAACAGGCAACAACAACCGATGAATTGGCTTTCACAAGGCCGTATGGCGGGCAGGGGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg                                                                                                                                                                                 <  2:171571/295‑1 (MQ=255)                                                                                                        aCAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGGGATAACTGGCCCGGAaggcagcacg                                                                                                                                                                             >  2:76302/1‑287 (MQ=255)                                                                                                             gaaaaaaaacCGGTGAAATGGGTTCAAAAGGGCCGTATGGCGAGTAGGAGAAGCAAA‑TTGTGACGGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGt                                                                                                                                                                        <  2:328171/288‑1 (MQ=255)                                                                                                                 aacaacCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATTCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGCCCGCCGCGTAGAGCTACCTGGCCCGGTCGATCGCAAGATGGTGATc                                                                                                                                                                    >  2:513990/1‑295 (MQ=255)                                                                                                                         aTGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATCTTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAACTTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGCGCGCAAGATGGTGCTCAACGCGCt                                                                                                                                                            >  1:427391/1‑295 (MQ=255)                                                                                                                            aaCTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGAGTCGCGCTCACTGCGCAGGTAGGGCGCACAGTGGTGCTCaatggcctaca                                                                                                                                                         >  2:739596/1‑286 (MQ=255)                                                                                                                                ggcgttccgaaGGCCGGATGGCGAGTAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGcc                                                                                                                                                     <  2:176039/286‑1 (MQ=255)                                                                                                                                ggCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGcc                                                                                                                                                     <  1:421099/295‑1 (MQ=255)                                                                                                                                   tttCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAAt                                                                                                                                                  >  1:498428/1‑295 (MQ=255)                                                                                                                                     gtacaAGGCCGGATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGACGAAGCGTGAGAAGTGCTGACGTAGCTGGGTACGAATGTTACGCCACAACGCAAGAAACTGCGTGCAGCGCGTATTCAGCATAAGCAATATATTGAGAACGGAACGTTGACTTATTTTATTTCGGAAACAGCTTCAATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAAtgt                                                                                                                                                <  2:427391/293‑1 (MQ=255)                                                                                                                                        caAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGaa                                                                                                                                             >  1:394808/1‑295 (MQ=255)                                                                                                                                              cGTATGGGGAGAAGGAGAAGCAAA‑TTCTGACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGGGACGCATTTTACGCCACAACGCAATAAACTGCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCtt                                                                                                                                       <  2:25331/295‑1 (MQ=255)                                                                                                                                               gTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGGGATCAACGCGCTCAACGCCAATGTGAAAGTCttt                                                                                                                                      >  1:292433/1‑295 (MQ=255)                                                                                                                                                 aTGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTAt                                                                                                                                    <  1:104772/295‑1 (MQ=255)                                                                                                                                                          agtagaAGCAAAGTTGTTA‑TGCCGAATGGGTAGAAGTTCACACTGAGCTGGTGACGCATTTTACGCCAAAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGAttt                                                                                                                           <  2:364614/292‑1 (MQ=255)                                                                                                                                                          aGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTGCTGACTGAGATGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCAGTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGAttt                                                                                                                           <  1:166204/295‑1 (MQ=255)                                                                                                                                                             agaAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGa                                                                                                                        <  1:463188/295‑1 (MQ=255)                                                                                                                                                                       ttcttcCTGCCTAAGCGGGAGAATTTCGGACTGGGCTGGGGACGCATTTTACGCAACAACGAAATACACTTCTGGCAGGGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACt                                                                                                               <  2:755337/295‑1 (MQ=255)                                                                                                                                                                       ttCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACt                                                                                                               <  2:721167/295‑1 (MQ=255)                                                                                                                                                                       tgtttATTGCCCAGGCGGTTGAATTTCGGACTGAGCGGTTGACTCATTTTAAGCCACAACGCAATAAACTACTGGCAGCGCGCAGTCAGCAGCAGCAAGTTATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACt                                                                                                               <  2:775941/292‑1 (MQ=255)                                                                                                                                                                            aCTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTTCGGACTTAGAAGACCGCCGCGTAGAGTTAACTGACCCGGTAGCGCGCAAGATGGGGATCAAGCCGCCCAACGCCAATGTGAGAGTCCTTCTGGCCGTTTTCGAAGATTAGCTGGcgc                                                                                                          >  2:16470/1‑293 (MQ=255)                                                                                                                                                                                 cGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGAc                                                                                                     >  1:705244/1‑295 (MQ=255)                                                                                                                                                                                       ggTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAAc                                                                                               <  1:627103/295‑1 (MQ=255)                                                                                                                                                                                       ggTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAAc                                                                                               <  1:262162/295‑1 (MQ=255)                                                                                                                                                                                       ggTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAAc                                                                                               >  1:233613/1‑295 (MQ=255)                                                                                                                                                                                          aGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACaaa                                                                                            <  1:305375/295‑1 (MQ=255)                                                                                                                                                                                            aaTTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGt                                                                                          <  1:310155/295‑1 (MQ=255)                                                                                                                                                                                                 ctgactgaGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGAACAACGCGCTCAACGCCAATGTGAAAGTCTTTCTGGCCGCTTTCCAAGATTCCCTGGCACCAGAACGGAACACAGTGATCg                                                                                     >  2:712526/1‑295 (MQ=255)                                                                                                                                                                                                   gactgaGCTGGTGACGCA‑TTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACg                                                                                  <  2:121203/295‑1 (MQ=255)                                                                                                                                                                                                    actgaGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACg                                                                                  <  1:442533/295‑1 (MQ=255)                                                                                                                                                                                                        aGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCa                                                                              >  1:151448/1‑295 (MQ=255)                                                                                                                                                                                                            ggTTACACATTTTAAGCCACAACGCAATAAACGTCTGGCAGCGCGCATTCAGCATGAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAAtt                                                                          <  2:610346/295‑1 (MQ=255)                                                                                                                                                                                                            cgTGACGCACTTAAAGCCAATCCGATATAAACGTCTGGTAGCGCGCAGTTAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAAtt                                                                          <  2:408795/294‑1 (MQ=255)                                                                                                                                                                                                             gTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTa                                                                         >  1:247812/1‑295 (MQ=255)                                                                                                                                                                                                                 cccATTTTCGGCCCCACCGCAATAACCTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCt                                                                     <  2:292433/293‑1 (MQ=255)                                                                                                                                                                                                                          ggcccaaaCGCATTAACCTTCTGGCGGCGCGCAGTCAGCAGCAGCAATATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGc                                                            <  2:84857/289‑1 (MQ=255)                                                                                                                                                                                                                               caACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTAAACGCCAATGTTAAAGTATTTATGGCCGATTTCGAAGATTCACTGGCACACGCCTGGACCAAAGTGATCGACGGGCAAATTAACCTGCGTGACGCGGTTa                                                       >  2:603504/1‑295 (MQ=255)                                                                                                                                                                                                                                     aaTAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCCCTGGCACCAGACTGGAACAAAGTGATCGACGGGCCAATTAACCTGCGTGATGCGGTTA‑ACGGCa                                                >  1:412115/1‑295 (MQ=255)                                                                                                                                                                                                                                      aTAAACTTCGGGCAGCGCGCAGTCAGAAGAAGCAAGATATTGAGAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCAc                                               <  2:155228/295‑1 (MQ=255)                                                                                                                                                                                                                                          aCTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACAGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCCAGATGGTGATCAACGCGCTCAACGCCAATGTGACAGGCTTTATGGCCGATTTCGAAGATTCCATGGCACCAGCCTGGAACAAAGTGATCGAAGGGCAAATTCACCTGCGTGATGCGGTTA‑AAGGCAcactc                                           >  2:514233/1‑291 (MQ=255)                                                                                                                                                                                                                                              cTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGtt                                       <  1:712526/295‑1 (MQ=255)                                                                                                                                                                                                                                                   aGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTACAcc                                  >  1:256323/1‑295 (MQ=255)                                                                                                                                                                                                                                                   aGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTACAcc                                  >  1:177721/1‑295 (MQ=255)                                                                                                                                                                                                                                                   aGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCAGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCCACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTCACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTacaca                                  >  1:414221/1‑294 (MQ=255)                                                                                                                                                                                                                                                       cgcATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTACACCAATg                              >  1:606743/1‑295 (MQ=255)                                                                                                                                                                                                                                                        gcATTCAGCAGCAGCAAGATATTGATAACGGAACGCTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGCAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCCGTGTGAAAACACTTATGGCCGATCTCGAAGCATCATTGGCAGCAGACTGGAACAAAGTGAGTGACCGGCACATTAACCGTCGTGATGCGGTTACACGGCACCCTC‑GTTACACATATGa                             >  2:171908/1‑295 (MQ=255)                                                                                                                                                                                                                                                          aTTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAACGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATAAGTTACAACAATGacg                           >  1:575115/1‑293 (MQ=255)                                                                                                                                                                                                                                                              agcagcagcAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGCCTGGAACAACGTGATCGCCGGGCAACTTAACCACCTTGATGCGGTTA‑ACGGCACCCTCAGTTCCACCAATGAAgacgg                       >  2:691737/1‑291 (MQ=255)                                                                                                                                                                                                                                                                   cagaaAGATATTTGTAACTTAACGTTGCCGTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAAGTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTACACCAATGAAGCAGGCaaaa                  <  2:498428/295‑1 (MQ=255)                                                                                                                                                                                                                                                                       aaGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTa              <  1:120072/295‑1 (MQ=255)                                                                                                                                                                                                                                                                         agtatTGAAATCGGAACGTTGCCGAGTTTCATTTCGGAAAAAGGTTCCATTGGCTATGCGGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTAcc            <  2:664753/293‑1 (MQ=255)                                                                                                                                                                                                                                                                           tatTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTACAACAAAGAAGCAGGAAAAATTTAACAg          >  2:379223/1‑295 (MQ=255)                                                                                                                                                                                                                                                                             ttGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCt        <  2:394808/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aagggAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTGGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTGAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGc   <  2:11441/292‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aCGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGCCGGGCAAATTACCCTGCGTGATGCGGTTA‑ACGGCCCCATCCGTGACACCACTGAAGCAGGCAAAATTTACCAGcaacagcc  >  2:120807/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                          TAAATGAGTAGTCTTAGTTGTGCTGAACGAAAAGAGCACAACGATCCTTCGTTCACAGTGGGGAAGTTTTCGGATCCATGACGAGGAGCTGCACGATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAGGAGAAGCAAA‑TTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTTATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTTA‑ACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCC  >  NZ_LN832404/4211856‑4212423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑