Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NZ_LN832404 | 4,613,143 | G→T | 88.9% | L133I (CTA→ATA) | EK12AG100_RS22840 ← | YjjI family glycine radical enzyme |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_LN832404 | 4,613,143 | 0 | G | T | 88.9% | 266.3 / 19.4 | 89 | L133I (CTA→ATA) | EK12AG100_RS22840 | YjjI family glycine radical enzyme |
Reads supporting (aligned to +/- strand): ref base G (4/6); new base T (42/37); total (46/43) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.13e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01 |
CCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTT > NZ_LN832404/4612852‑4613433 | ccccTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGGTTTATTGGAACATCGATCCCGTCTTGTGTTATAAt > 2:32269/1‑295 (MQ=255) ccTTTTGTGAAAATTTTTTCATGCACCGGACCGTGGGGGATGTGCGGTTTGCTACATTCACAGATGTTCCTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTc < 2:468845/295‑1 (MQ=255) tttGTGAAAATTTTTTCATGCACCGTACCGTTGGCGATGTGCGGTTTTCTACAGTCACAGATGTTCTTCGCCACGTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTaa < 2:309000/295‑1 (MQ=255) tgAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCGGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATa < 2:284381/295‑1 (MQ=255) gAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGACTCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCCGGCGGGGTAACGCCAGAAACGTTTTATTCGAACATCCATCTCGTCTTGTGTTCTAATTATAACATAc > 2:68664/1‑295 (MQ=255) aaTTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGt > 1:652095/1‑295 (MQ=255) tATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGcaa > 1:79094/1‑295 (MQ=255) aTGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCaacaac > 1:81007/1‑295 (MQ=255) aTGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTGCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCaacaac < 1:627029/295‑1 (MQ=255) aCCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCAt > 1:261880/1‑295 (MQ=255) ggACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCa < 1:390982/295‑1 (MQ=255) ggACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCa > 2:390982/1‑295 (MQ=255) ccgttgtcgtgttgCTGTTTTCTACATTCCCAGATGTTCTTTGCCCTGTCCAGAGGCAGTTCATCAGGGGGGATTTCAGTCTCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGT‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGtt < 2:697772/283‑1 (MQ=255) ttGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGcc < 1:508043/295‑1 (MQ=255) gATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAgg > 1:714513/1‑295 (MQ=255) aTGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTAGAGGTAACGCCAGAAACGATTTATTCGAACATCGATCTAGGACTGTGTTATAATTCTAAACTACGGTTGAAACACAGAATCAAGTTGCCCCAGGt > 2:540818/1‑295 (MQ=255) tgtgCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTCTAATTCTAACATAGGTTTGCACCAACGCATCCAGATTCACCAGTTa > 2:650280/1‑295 (MQ=255) cTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAAAAACGCATCCAGTTGCCCCCGGTCGACCGGCATCg > 2:626961/1‑295 (MQ=255) aTTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGAtgt < 1:304248/295‑1 (MQ=255) tCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGa > 1:683009/1‑295 (MQ=255) gcccggATGTTGTTCGCCACTGCCAGCAGCAGGTCATCAGGGGTGATGTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACGCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGa < 2:198626/290‑1 (MQ=255) acaGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGCAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGACACAACGCACCCAGTTGCCCCAAGTAGACACGCATAGAtggtaac > 2:703680/1‑290 (MQ=255) caGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGACCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATACTTCTAAACTACGTTTGCAACAACGCATCAAGTTGCCCATGTTAGACCGGCCTACATGTGCCCg > 2:514129/1‑295 (MQ=255) aaaggTTCTTGACCAATTCCAGCAGCGGGGCAATAGGGGTGATTGCAGAATCGTAGAGAAAGGTCATGTTCGGTGAAACCGGCTTCAACTCTGCAACTGCACGTAAGATCGCGCGGGTAATGG‑GCGAAGCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGa < 2:384464/291‑1 (MQ=255) tcttcGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTTGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTAc < 2:308439/295‑1 (MQ=255) cGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACgtgg < 1:341104/295‑1 (MQ=255) cagcagcagGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGAGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGGAGTAACGACAGAAACGTTTTATTCGATCATCGCTATCGTCTTGTGTTAGCATTCTAACATCAGGTTGAAACAACGCATCCAGTGTCCACCGGTCGACCGTATACGACGAGAACGACTGTTCATGGTGGTcaata > 2:438738/1‑293 (MQ=255) gcagcagGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAAt < 1:38193/295‑1 (MQ=255) cagcagGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATg > 1:111880/1‑295 (MQ=255) agcagGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATgg > 1:598002/1‑295 (MQ=255) agGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGGAAAGAATGGTca > 1:327647/1‑295 (MQ=255) ggatagaGGGGTGTTTTAAGGTAAGTAGATAAAGGGAAGTGTGGTGGAAACCTGGTTAAAGTCTGCACCTGCACGAAAGATCGAGCGGTTAATGG‑GCGAATCAGACGGGCCGAGATTGGCGTGCATAAAGGCGTCGGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTcagc < 2:5996/289‑1 (MQ=255) tcatcaGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTcagca < 1:254986/295‑1 (MQ=255) aGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCagagag < 1:483339/295‑1 (MQ=255) cAGGATCGTATATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGtcatc < 1:442068/295‑1 (MQ=255) aGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGACACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACCTACGGTTGCCACAACGCATCCAGTTGCCACAGGTAGACCGGCATCGATGTGACCGACCGTCCGTGGTGGGACAGAATGGTACGCAGCGCGCGTG‑CGtcatca > 2:585925/1‑295 (MQ=255) aTCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGGATGGTCAGCAGAGAGAGTC‑CGTCATCAAgc > 2:39311/1‑294 (MQ=255) cGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATTTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGCCGGTACGTTGTGGTAAAGAATGGTCAGCAGAGA‑CGTGTCGGCATCAAGATc > 2:480763/1‑295 (MQ=255) gATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGCTCTTTc > 1:467081/1‑295 (MQ=255) aGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTGTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGAATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGCGGTAACGAATGGTCAGCAGAGCGAGTG‑CGACATCAAGACCTGTCgcgca > 2:145844/1‑294 (MQ=255) cAGGTTCGGGGAAACCTGCTTCAACTCGGATTCTGCACGTAAGATCGCGCGGTTAATGT‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCATGGTTCTGTCGAGGTAACGCCAGAACCGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTtcc < 2:683009/295‑1 (MQ=255) aGGTTCGGTGAAACCTGCTTCAATGCGGCCTCTGCACGTAAGAGTGCGCGGGTAAGGG‑GCGAAGCAGACGGGCCGAGATTGGCGTGCATAAAGGCGTCGGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTtcca < 2:81007/295‑1 (MQ=255) aGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTtcca > 1:188092/1‑295 (MQ=255) ttCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGTATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTT‑CGTCATCAAGATCTTTCGCGCCTtccagct > 1:473336/1‑295 (MQ=255) ttCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGAAACAACGCATCCAGTTGCCCCAGGTAGAACGGCATCGATGTGACCGACGGTACGTGGTGGTGAAGAATGGTCAGCAGAGAGAGGG‑CGGCGTCAAGATCTTGCGCGCCTtacagac > 2:395369/1‑293 (MQ=255) cGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATATGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTtccagctcc < 1:405238/295‑1 (MQ=255) ggTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCCTCGATGTGACCGACGGTACGTGGTGGTAGAGAATGGTCAGCGGAGAGAGTG‑CGTCATCAAGCTCTTTCCCTCCTtcgagcacta > 1:762188/1‑291 (MQ=255) gTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCTATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTGGCGCCTtccagctaccg > 1:313190/1‑291 (MQ=255) gaacgccTGCTGCAACCCGGCATGTGCAGGTAGTGCCGCGC‑GGTCATGGAGCGACTCAGACGGGCCGATATTTGCGTGCAGACATGCGTCTGGCATGTTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTtccagctccagc < 2:209237/290‑1 (MQ=255) aaaCCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCa < 1:280901/295‑1 (MQ=255) ttCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGCAc > 1:100189/1‑295 (MQ=255) tCAACTCTGGAGTGGCACGTAAGATGGCGCGGGTAATGT‑GCGGATCAGCCGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAAcc < 2:52751/295‑1 (MQ=255) aaCTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACCACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGt > 2:109930/1‑295 (MQ=255) ctgcatctgcaCGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGCTCTTTCGCGCCTTCCAGCTCCCGCCCTTCGGACCCGGTCGc > 2:322060/1‑295 (MQ=255) catctgcaCGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAg > 1:260672/1‑295 (MQ=255) tctgcaCGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGaa > 1:582916/1‑295 (MQ=255) gcaCGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAAc < 1:97102/295‑1 (MQ=255) aaGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGTTAGACCGGCATCGATGTGACCGACGGTACGTGGTGTTAACGAATGGTCAGCAGAGAGCGTG‑CGTCATCCAGATCTTCAGCCCCTTCCAGCCCCAGCCCTCACGAACCGTTCGCCaacaaaaggcggg > 2:613758/1‑283 (MQ=255) aaGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGt > 1:655716/1‑295 (MQ=255) gATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGACTGGTCAGCAGAGAGAGTG‑CGTCCTCAAGATCTTTCGCGCCTTCCAGCTCAAGCCATTCGGAACCGTTCCCCAGCCACCGGGCgaca > 2:139593/1‑292 (MQ=255) gctgggaaagg‑gCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGT‑ACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTa < 2:714513/286‑1 (MQ=255) gTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGTTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAACGAATGGTCAGAAGAGCGAGTG‑CGTCATCAAGACCTTTCGCCCCCTCCAGCACCCGCCATTCGGAACCGCTCGCCCGTAACGCGGCCGAATCGGGTaaaa > 2:744284/1‑293 (MQ=255) cAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGttt < 1:32269/295‑1 (MQ=255) gATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCa < 1:650280/295‑1 (MQ=255) gATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCa > 1:330244/1‑295 (MQ=255) ttatTGTCGTGCATAAAGGCGTCTGGCAGGGTGCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATTTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCAt < 2:79094/294‑1 (MQ=255) tggttggCATAAAGGTGTCTTGAAGTGTGCTGTCGAGGTAACGCAAGAAACGTTTTATTCGACCAACTATCTCGTCTTGGGTTATAATTCTAACATACGGGTGCAACAACGCATCCAGTGGCCCCAGGTAGACCGTCATCGAGGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTGCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGAcc < 2:489506/289‑1 (MQ=255) ggCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCt < 1:406614/295‑1 (MQ=255) gTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGCATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAACGCGGTTTGTACGGCCCCTGGCCTTcc > 2:724043/1‑294 (MQ=255) tGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCaa < 1:291279/295‑1 (MQ=255) tAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACat < 1:644576/295‑1 (MQ=255) tAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACat < 1:139593/295‑1 (MQ=255) aaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGACTGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTACCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGAATGACCTTACAACatat > 2:710997/1‑295 (MQ=255) aaGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACAGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACatat < 1:240872/295‑1 (MQ=255) ggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAACCGGGCGTAATCGGGTAAGACCTAGCGCGGTTTGTACGGCGCATGACCTTCAAACATACCGCATCTTACACCt > 2:426153/1‑295 (MQ=255) tCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCa < 1:626961/295‑1 (MQ=255) cGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGACCCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAAACTACAGCAGATTACACCTTCATCAAg > 2:419338/1‑295 (MQ=255) cGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAg < 1:540818/295‑1 (MQ=255) cGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAg < 1:724043/295‑1 (MQ=255) gAGGTAACGCCAGAAACGGTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAgc < 1:514129/295‑1 (MQ=255) tAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAgcgcgc < 1:322060/295‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCTCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAgcccgccg > 2:205573/1‑293 (MQ=255) cGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCgcggc < 1:735625/295‑1 (MQ=255) aGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGc > 1:481221/1‑295 (MQ=255) aaaCGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCCGCGCGCGGCGGGCtt > 1:530034/1‑295 (MQ=255) aaaCGTTTTATTCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGACAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCATTCAAGCTACAGCCATTCGGAACCGTTCGCCCGAAAACGGGCGTAATCGGGAAAGACAGAGCGCGGGTAGTACGGCGGATGACCTTAACACATATCGCCGCATACACCATCATCCAGAGCGCGGAgggact > 2:209923/1‑292 (MQ=255) tttttATTCGAACATGGATCTCGGCTTGTGTTATAATCCTAAGTTACGGTGGCAACAACGCATCCAGTTGCCCCATGTAGACCTGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCATAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGc < 2:564136/294‑1 (MQ=255) gTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGc < 1:39311/295‑1 (MQ=255) tCGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGc > 1:498784/1‑295 (MQ=255) cGAACATCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCt < 1:145844/295‑1 (MQ=255) aTCGATCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTggg > 1:434062/1‑295 (MQ=255) aTCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAg < 1:585925/295‑1 (MQ=255) aTCTCGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAg > 1:642097/1‑295 (MQ=255) ctacTTGTGTTATAATTCTAACATACGGGTCCAACCATGCATCCGGTTGCCCCCGGTCGGCCGGCATAGATGGGACCGACGGTACTGGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCGCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAg < 2:195946/292‑1 (MQ=255) cGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCCCCAGCCATTCGGAACCGTTCGCCAGAACACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCCAATTACACCTTCATCCAGCGCGCGGACGGCTTCGGCAGGAAGCTGGGGGGAAGGCAg > 2:74793/1‑295 (MQ=255) cGTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACAGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGACTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACATTCAAACATATCGAAGATACCACCTTCCTCCAGCGCGCGGCGGGCTTCGGAAGGAAGCTGGGGGTAAGGCAg > 2:498542/1‑295 (MQ=255) gTCTTGTGTTATAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGACGCTGTAGGTAAGGCAGa > 2:346799/1‑295 (MQ=255) cTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGCACCGTTCGACAGCAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATCACACCTTCCTCCAGAGCGCGGCGGGCTTCGGCAGGCACCTGTGGGTAAGGccgcat > 2:518342/1‑290 (MQ=255) tgtggAATTCTAACATACGGTTGCAACAGCGCAGCACGTTGCCCAAGGTGGACCGGCATCGAGTTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGGGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGtttt < 2:181487/291‑1 (MQ=255) | CCCCTTTTGTGAAAATTTTATCATGCACCGGACCGTTGGCGATGTGCGGTTTGCTACATTCACAGATGTTCTTCGCCACTTCCAGCAGCAGGTCATCAGGGGTGATTTCAGGATCGTAGATAAAGGTCAGGTTCGGTGAAACCTGCTTCAACTCTGCATCTGCACGTAAGATCGCGCGGGTAATGG‑GCGAATCAGACGGGCCGATATTGGCGTGCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCAGGTAGACCGGCATCGATGTGACCGACGGTACGTGGTGGTAAAGAATGGTCAGCAGAGAGAGTG‑CGTCATCAAGATCTTTCGCGCCTTCCAGCTCCAGCCATTCGGAACCGTTCGCCAGAAAACGGGCGTAATCGGGTAAGACATAGCGCGGTTTGTACGGCGCATGACCTTCAAACATATCGCAGATTACACCTTCATCCAGCGCGCGGCGGGCTTCGGCAGGAAGCTGTGGGTAAGGCAGATTGTTTT > NZ_LN832404/4612852‑4613433 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |