Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	1,639,016	G→A	23.7%	H162H (CAC→CAT)	EK12AG100_RS08165 ←	tail fiber protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	1,639,016	0	G	A	23.7%	181.1 / 48.8	84	H162H (CAC→CAT)	EK12AG100_RS08165	tail fiber protein
Reads supporting (aligned to +/- strand):  ref base G (31/33);  new base A (6/14);  total (37/47)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.77e-01

ACCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAA  >  NZ_LN832404/1638728‑1639310                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                       aCCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGAt                                                                                                                                                                                                                                                                                                  >  1:551619/1‑295 (MQ=255)  ccATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTTTGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGGTGTGGTTTCCGTCCACAAATCCGT‑ACTGGATGCGCTGGAGTTGTGGGTGTGCGAtt                                                                                                                                                                                                                                                                                                 >  1:307519/1‑295 (MQ=255)  ccATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGAtt                                                                                                                                                                                                                                                                                                 <  1:37703/295‑1 (MQ=255)    aTCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGAACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTa                                                                                                                                                                                                                                                                                               >  1:681841/1‑295 (MQ=255)         atgatgTCTTCCCTGAATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCACCGCGCTTAGATTTGAAATAGCGGTATAACCATTAGGGAAAATGATCGTGTTCGTGCCACCAAATGCACCGGAACTCGTGTGTTGGTGCGCACTGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCACCGGTGTTATTCGTGGATTTGGTTACGTAATAAAACGAAGATGTGGTTTCCGTCGTCAAGTACGT‑ACTGAATGCGCTGGCCCTGTGGGTCTGCTGTTTCATgtg                                                                                                                                                                                                                                                                                          >  2:352773/1‑293 (MQ=255)             tgTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTcc                                                                                                                                                                                                                                                                                      <  1:770243/295‑1 (MQ=255)                   cccTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTcc                                                                                                                                                                                                                                                                                >  1:543348/1‑295 (MQ=255)                         aTTCCGAGGCTGGCCACTCCCGCTTGTTGTGCTCATAATCCCCGTGTTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGat                                                                                                                                                                                                                                                                          <  2:789408/295‑1 (MQ=255)                           tACTAGTCGGGCCCCTTCCGCTGGTGGGGCTCATAACCCCCTCGCTTAGATTGGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCTTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGataa                                                                                                                                                                                                                                                                        <  2:589704/295‑1 (MQ=255)                             cGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGataata                                                                                                                                                                                                                                                                      <  1:884450/295‑1 (MQ=255)                              gAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTTCTGAGataattc                                                                                                                                                                                                                                                                     >  1:185085/1‑293 (MQ=255)                                    ggCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCg                                                                                                                                                                                                                                                               <  1:894102/295‑1 (MQ=255)                                         cTACCGCTGTGTGGGCTCAGAATCCCGTCGCTTAGATTTGAAATGGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCAc                                                                                                                                                                                                                                                          <  2:872760/295‑1 (MQ=255)                                                ttgttgTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCggg                                                                                                                                                                                                                                                   <  1:843322/295‑1 (MQ=255)                                                  gtggtgGTCATAATCCCCGCGTGTAGATTTGAAATGGCTGTATAACTATAAGGGAAGAAGCTCGATATAGTTACAACAAATGTACCAGAACTCTTGGATTGTGGGGGACCGGAACTATTAGCTGTCCCGCTAATACTATGGGTATGCGCCTCGTTGTTATTCGTAGATTTGTTTCCGTAATCAAACGACGATGAGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCt                                                                                                                                                                                                                                                 <  2:621385/292‑1 (MQ=255)                                                    tgtgCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGTGCTTg                                                                                                                                                                                                                                               >  1:430162/1‑295 (MQ=255)                                                         tCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGCTTTAATGCCGTCCTGTTCCTGAGATAATCGGGCACGACCACTGGAGGCCTTGCCCta                                                                                                                                                                                                                                          >  2:764843/1‑294 (MQ=255)                                                           aTAATCCCCGCGCTTAGATTTGAAATCGCTGTATAACCATTAGGGAAAATGCTCGGGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGAGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAAGACGACCCGACCACTGGCGGGCTTGCCCTTaa                                                                                                                                                                                                                                        <  1:457326/295‑1 (MQ=255)                                                                   cgcgCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGCTTTAATGCCGTCCTGTTCCTGAGATACTCCGGCCCGACCACTGGCGGGCTTACCCTCACACGTCCAg                                                                                                                                                                                                                                >  2:573021/1‑295 (MQ=255)                                                                      gcTTAGATTCGACATTGGGGTAACACCATTAGGGAGATTGCTCGTGTTCGTTCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCa                                                                                                                                                                                                                             <  2:551619/295‑1 (MQ=255)                                                                        ttAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCACAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTACTGCCGTCCTGTTCCTGAGATAATACGGCCCGACCCCTGGCGGGCTTGCCCTTCATCGTCCCGCCACg                                                                                                                                                                                                                           >  1:404020/1‑295 (MQ=255)                                                                             ttttAAATCGCGGTATAACCTTAGGGGAAAAGGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGGACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCatat                                                                                                                                                                                                                      <  2:748535/295‑1 (MQ=255)                                                                                tgaattgggttctaACCATTAGGGAACTTGCTCGGTGTCCTGCCCCCAAATGCCCCGGAACCCTTGGTTTGGTGCGCACCGGAACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAg                                                                                                                                                                                                                   <  2:185085/283‑1 (MQ=255)                                                                                       cgGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCAc                                                                                                                                                                                                            >  1:12444/1‑295 (MQ=255)                                                                                       cgGTAGAACCATTAGGGAAAATGCGCGTGGTCGTGCTACCAAATGCACCGGAACTGTTGTTTTGGTGCGCACCGGCACTAGTTGCGGTCCCGTTAATACTATGGGTATGCTCTCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACTATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCAc                                                                                                                                                                                                            <  2:681841/295‑1 (MQ=255)                                                                                         gTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGCGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGc                                                                                                                                                                                                          >  1:765857/1‑295 (MQ=255)                                                                                           ataACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCTTGAGATGATACGGCCCGACCACTGGCGGGCTTGCGCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCt                                                                                                                                                                                                        >  1:678321/1‑295 (MQ=255)                                                                                              aCCATTAGGGAAAATGCTCGTGGTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGTGCACCGGCACTATTTGCGGTCCCGCTAATACTATGTGTATGCGCCCCGGTGTTATTCGTGTATTTGGTTGCGGAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGTTGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGAc                                                                                                                                                                                                     <  1:873549/295‑1 (MQ=255)                                                                                                  tttgggAAAATGCTCGGGTCCGGGCCACCGCATGCCCCGGAACTCTTGTGTTGGTGCGCACCGGCACGATTTGCGGTCCTGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGAt                                                                                                                                                                                                 <  2:12444/292‑1 (MQ=255)                                                                                                     gggAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCTAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATCATACGGCCCGACCACTGGCGGGCTTGCCCTTTATCGTCCAGACACGCCTAACAGGGACTAGGCCTGGAGGATAAg                                                                                                                                                                                              >  2:663562/1‑295 (MQ=255)                                                                                                          aaTGCTCGTGTTCGTGCCACGAATGGGACCGGATGTCTTGTGGTGGTGCGCGCCGGCACTATTTGCGGTGCCGCTATTACTATGGGTATGCGCCCCGGTGTTATTCGTGTATTTGGTTCCGTAATCTAACGACGATGTGGTTTCCGTCCCCGAATCCGT‑ACTGGATGCGCTGGCGCTATGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACt                                                                                                                                                                                         <  2:105627/295‑1 (MQ=255)                                                                                                                      cGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCggg                                                                                                                                                                             <  1:419856/295‑1 (MQ=255)                                                                                                                        gtcaagcacaTGCACCGGAACTCTGGGGTTGGTGCGCAGCGGCACTATTTGGGGTGCCGCTAATACTTTGGGTATGCGCCGCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGGTGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTa                                                                                                                                                                           <  2:613094/286‑1 (MQ=255)                                                                                                                         gCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGTTAATACGGCCCGACCACTGGCGGGCTTGCACTTACTCGTCCAGGCACGCATATCCGGGACCACGCCTGCCGGACACGCTCCTGAACGTTTCGGGTAt                                                                                                                                                                          >  2:264735/1‑295 (MQ=255)                                                                                                                              caAATGGACGGGAGCTCTGGGGTGGGTGGTCACCGGCAGTATTTGCGGGCCCGCTAATACTATGGGTATGGGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGa                                                                                                                                                                     <  2:75189/295‑1 (MQ=255)                                                                                                                               aaaTGCATCGGAATTCTTGTGTTGGTGCGAACCGGAACTATTTGGGGTCCCGCTAATACTACTGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGAt                                                                                                                                                                    <  2:585291/295‑1 (MQ=255)                                                                                                                                aaTGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCTGGGATCACGCCTGAAGGATAAGCAACTGCAAGTTTCGGGTATGCAGAtt                                                                                                                                                                   >  1:207729/1‑295 (MQ=255)                                                                                                                                     aCCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGCTTTGTCa                                                                                                                                                              >  1:848165/1‑295 (MQ=255)                                                                                                                                         gAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAg                                                                                                                                                          <  1:134267/295‑1 (MQ=255)                                                                                                                                              ctTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGAGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTCATAGTCCAGCCACGACTAGCCGGGACCACGCCTGACGGATAAGCCACACAAAGTTTCGGGTATGCAGATTTGCAACAAGTATGc                                                                                                                                                     >  2:86318/1‑295 (MQ=255)                                                                                                                                                 gtgtTGGTGCGCACCGTCACTAGTTGCGGTCCCGGTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGAGGTGGTTTCCGGCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGcccc                                                                                                                                                  <  2:543348/295‑1 (MQ=255)                                                                                                                                                   gtTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCCCGCATATCAGGGATCACGCCTGGCGGGTAAGCCACTGCAAGTTTTGGGGAAGAGGATTTGTCACAAGTCCGcacccg                                                                                                                                                >  2:391148/1‑293 (MQ=255)                                                                                                                                                        ggccccccggtcgtATTTGCGGTCCTGCTACTACGTTGGGTATGCGCCCCGGTGTTGTTCGTGGTTTTTGTTCCGTAGTCAAACGCGGATGTGGTTTCCGTCGCCAAATCCGT‑ACTGTATGCGCTGGCGCTGTGGGTGGGCGATTTAGTGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAAGCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCa                                                                                                                                           <  2:720088/282‑1 (MQ=255)                                                                                                                                                           gcACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAggg                                                                                                                                        >  1:270173/1‑295 (MQ=255)                                                                                                                                                                  cACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCACTGAGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGACTTGTCAAAAGTCAGCCCATGCATCCGGGCATAAcc                                                                                                                                 >  1:584758/1‑295 (MQ=255)                                                                                                                                                                   aCTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCa                                                                                                                                >  1:181916/1‑295 (MQ=255)                                                                                                                                                                      gtttgtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGAc                                                                                                                             >  1:252414/11‑295 (MQ=18)                                                                                                                                                                          gtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGaa                                                                                                                         <  1:320091/289‑1 (MQ=18)                                                                                                                                                                          gtagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGaa                                                                                                                         <  1:563859/289‑1 (MQ=18)                                                                                                                                                                           tagagccGCTCACACTGTGTGTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGacc                                                                                                                        >  1:710068/6‑293 (MQ=18)                                                                                                                                                                                    tAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCCCGCCTGCCGGCTAAGCAACTGCAAGTTTCGGGTATGCAGATTCTTCAACAATCTGCCCCTGCACACGGGCTTACCCAGCCCGAAAGGtctcccc                                                                                                               >  2:473709/1‑292 (MQ=255)                                                                                                                                                                                     cacaCTGTGTGTATGTGCCCCGGTGTTCTTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                              <  2:450406/292‑1 (MQ=18)                                                                                                                                                                                     aaTACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                              >  1:181198/1‑295 (MQ=255)                                                                                                                                                                                         ctgtgtgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGcc                                                                                                          <  1:123077/289‑1 (MQ=18)                                                                                                                                                                                           gtgtgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCAc                                                                                                        <  1:601053/291‑1 (MQ=18)                                                                                                                                                                                            tGGGTATGCGCTACGGTGTTATTCGGGGATTTGGTTCCGTAATCAAACGACGATGTGGTATCCGTCCCCAAATCCGT‑ACGGGATGCGCTGGCGCTGTGGGTGTTCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGACCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATAACAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACg                                                                                                       <  1:853155/295‑1 (MQ=255)                                                                                                                                                                                              tgTATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGAAAGATTCGGGTACGCGGATTTGTCAAAGGCACGCCCCTGCATCAGGGCATAAGCAGACAGAGCGGGCTACGGTGGCCACggg                                                                                                     >  2:540262/2‑295 (MQ=18)                                                                                                                                                                                               ggaTGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGGAATCAAACGACGAGGTGGTTTGCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCATGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGa                                                                                                    <  1:881723/293‑1 (MQ=255)                                                                                                                                                                                                tATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                              <  2:719929/284‑1 (MQ=18)                                                                                                                                                                                                tATGTGCCCCGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGAt                                                                                                              >  1:719929/1‑284 (MQ=18)                                                                                                                                                                                                gttgtgcCCCGGTGTTATGCTGGTATTTCGTGCAGTAATCAAACGACTATGTGGTTTATGTCCCCAAATCTGT‑ACTGGATGCGCTGGGGCTGTTGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCATGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGAt                                                                                                   <  2:778982/290‑1 (MQ=255)                                                                                                                                                                                                      cgccGGTTTTAGTTGTGGATTGGGTTCCGTAATCAAAGGACGTTGTGGTTTCCGTCCCCAAATCCGT‑ACTTGATTCTCTGGCGCTGTGGGTGTTCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCTGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGc                                                                                             <  2:207729/293‑1 (MQ=255)                                                                                                                                                                                                      ccccGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAGTCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGc                                                                                             <  1:337688/295‑1 (MQ=18)                                                                                                                                                                                                         cGGTGTTATTCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCACGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCCTCAGGGCATAACCCGACGGAACGGTATCTGATTGCCACCGGATTgggcagac                                                                                          >  2:530580/1‑289 (MQ=18)                                                                                                                                                                                                            tgtTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATCTCAGGGATCACGCCTGACGGATAAGCAACTGCCAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCCTCACCAGACGGACCGGTCTCTGATGTCCACGGGGTTGGTGCCCCgct                                                                                       >  2:759117/1‑293 (MQ=255)                                                                                                                                                                                                            tgtTATTCGTGGATGTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAc                                                                                       <  1:624734/295‑1 (MQ=18)                                                                                                                                                                                                            tggtATTCGTGGATTTAGTTCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCCATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGAc                                                                                       <  1:641686/292‑1 (MQ=18)                                                                                                                                                                                                               tggtCGTGGGGTTGGTTCCGTAATCAAACGGCGATGTGGTTTCCGTCCCCAACTCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTgg                                                                                    <  2:63647/292‑1 (MQ=255)                                                                                                                                                                                                               tATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTgg                                                                                    >  1:748749/1‑295 (MQ=255)                                                                                                                                                                                                                  tCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCCTCACCAGCCGGAACGGTATCTGATGGCCACGGGGTTGGTGAACCGACTgggaa                                                                                 >  2:415851/1‑292 (MQ=255)                                                                                                                                                                                                                  tCGTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTGCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTa                                                                                 <  1:814240/295‑1 (MQ=255)                                                                                                                                                                                                                    gTGGATTTAGTGCCGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGGCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagc                                                                               <  1:259196/295‑3 (MQ=18)                                                                                                                                                                                                                      ggATTTAGGGCCGTAGTCGAACGACGATGTGGTTTTCGTCCCCAAA‑CGGTGGCTGGATGGGCGGGGGCTGTGGGTGTGCGATTTAAAACCGTCCTGTTCCTGAAGCAAGACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAGGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagctt                                                                             <  2:371027/295‑5 (MQ=255)                                                                                                                                                                                                                        atgtGGTGCCGTAAGTAAACGACGAGGTGGTTTCCGACCCCAGAGCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGAGTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTGAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAActc                                                                           <  2:848165/292‑1 (MQ=255)                                                                                                                                                                                                                         tttGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAActct                                                                          >  1:206335/1‑295 (MQ=255)                                                                                                                                                                                                                           tagtgccGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGAGGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTGCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCGCCGACTGGGTagctttctgg                                                                        <  1:610552/290‑10 (MQ=18)                                                                                                                                                                                                                            ggTTCCGTAAGCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCa                                                                       <  1:880980/295‑1 (MQ=255)                                                                                                                                                                                                                                ccGTAATCAAACGACGATGTGGTTTTCGTCCCCAAATCTGT‑ACTGGATGCGCTGGCGCTGTGGGTATGCGATTTAATGCCGTCCTGTTCCTGAGACAATACGGCCCGACCACTGGCAGGTTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCGGCTGCAAGTTTCGGGTAAGCAGATTTGTCAAAAGCCTTCCCCTGCATCAGGGCATAACCAGACGTAACGGTATCTGATGGCCACGGGATTGGTGCGCCGATTGGGTagctttctggtggaa                                                                   >  1:491521/1‑281 (MQ=18)                                                                                                                                                                                                                                  tgtataaaatgagcaTGTGGTTTGCGTCCCAAGTGCCAT‑ACTGGATTCGCTAGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATAACGCCCGACCACTGGCGTGCGTGCCCTTAATCGTCCAGCCACGCATATCAGGGATAACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAggagga                                                                 <  2:881442/281‑1 (MQ=255)                                                                                                                                                                                                                                             acgaTGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTCATCTTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCCGATTTGTCAAAAGTCTGCCCCTGCCTCCGGGCATAACCAGACGGAACGGTATCCGCTGGCCACGGGATTTGTGCACCCACCGGCACCAAATCTGACGGAGGATGAGCCGAggg                                                      >  2:636801/1‑294 (MQ=255)                                                                                                                                                                                                                                                aTGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACTCATACCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTATGCACCTGCATCAGGTCATGAACAGACGGAACGGTATCTGATGGCCACGGGTTCGGTGCCCCCGCTGCATAAAACGTTGCTGGAGGACGCGCCGATGGGTa                                                   >  2:106023/1‑295 (MQ=255)                                                                                                                                                                                                                                                    ggTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAAATCTGCAGGAGGATGAGCCGGGGTGGAAAGc                                               >  1:150801/1‑295 (MQ=255)                                                                                                                                                                                                                                                           gTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGGCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGccc                                        <  1:224281/295‑1 (MQ=255)                                                                                                                                                                                                                                                                gcaaTCCGG‑AGTGGGTGCGTGGGGGGTGTGGGTGTGCGATTGAAGGCCGTCCTGCTCCTGGGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGc                                   <  2:301438/293‑1 (MQ=255)                                                                                                                                                                                                                                                                        ggtcTGGATGCGCTGGCGCGGTGGGTGTGCGTGTTAATGCCGTCCTGTTCCGGCGATAATACGGCCCGACCCCTGGCGGGCTTTCCCTTAAACGTCCAGCCAGGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGt                            <  2:605009/292‑1 (MQ=255)                                                                                                                                                                                                                                                                               aTGCGCTGTGTCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAAGACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtc                     <  1:264735/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                 gcgctggcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGGGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcgg                   <  1:859721/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gcttgcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGTCTTGCCCTTAATCGTCCAGCCACGCATATCAGGAATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggtc                 <  1:86318/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gctggcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTAGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGTGATCACGCCTGACGAATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCgtcggtc                 <  1:869581/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                    ctggcgctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATACAACTCTGCAGGAGGCTGAGCCGAGGTGTAAAGCTGCCCCCACGGCCACCAGTTTGCgtcggtcg                >  2:595226/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                          ctgTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATccc          >  1:29904/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                                  gtgCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTCAAGCTGCGCCCACGGCGACCAGTTTGCGCCGGTCGTATCCcgtcggtaa  >  2:609423/1‑291 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                       ACCATCTGATGATGTCTTCCCTGCATTACGAGTCTGGCCACTACCGCTTGTTGTGCTCATAATCCCCGCGCTTAGATTTGAAATCGCGGTATAACCATTAGGGAAAATGCTCGTGTTCGTGCCACCAAATGCACCGGAACTCTTGTGTTGGTGCGCACCGGCACTATTTGCGGTCCCGCTAATACTATGGGTATGCGCCCCGGTGTTATTCGTGGATTTGGTTCCGTAATCAAACGACGATGTGGTTTCCGTCCCCAAATCCGT‑ACTGGATGCGCTGGCGCTGTGGGTGTGCGATTTAATGCCGTCCTGTTCCTGAGATAATACGGCCCGACCACTGGCGGGCTTGCCCTTAATCGTCCAGCCACGCATATCAGGGATCACGCCTGACGGATAAGCAACTGCAAGTTTCGGGTATGCAGATTTGTCAAAAGTCTGCCCCTGCATCAGGGCATAACCAGACGGAACGGTATCTGATGGCCACGGGATTGGTGCACCGACTGGATAAAACTCTGCAGGAGGATGAGCCGAGGTGTAAAGCTGCGCCCACGGCGACCAGTTTGCGTCGGTCGTATCCCGTCGTGAA  >  NZ_LN832404/1638728‑1639310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑