Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	578,168	C→A	100%	L132I (CTC→ATC)	EK12AG100_RS02885 →	DNA‑packaging protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	578,168	0	C	A	100.0%	126.4 / ‑6.7	47	L132I (CTC→ATC)	EK12AG100_RS02885	DNA‑packaging protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (26/20);  total (27/20)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGCACAGGAACTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGTTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGCTCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCC  >  NZ_LN832404/577880‑578459                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                     gggaaTGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATccccc                                                                                                                                                                                                                                                                                                <  1:710455/292‑1 (MQ=9)  gTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCt                                                                                                                                                                                                                                                                                               <  1:415000/295‑1 (MQ=9)   tAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGCTCCCCCTg                                                                                                                                                                                                                                                                                              >  1:742034/1‑295 (MQ=16)    aaTGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTCCGCGTGCGCAGCCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTACCGGATCGCCGGTGAGACTGCCAGTATTCTCGACGGGATCCCGCTGt                                                                                                                                                                                                                                                                                             >  2:677612/1‑295 (MQ=11)         ggTGCTTTATGACTCTGCCGCCGTCATAAACTGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCACGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGATGTGGAGGAAACCGCATTCTGTGCGTTCGTGCTGTCGCGGATCGCACGTGCAATTGCCAGTATTCCAGACGGGATCACCCCTTCGGtc                                                                                                                                                                                                                                                                                        >  2:726857/1‑294 (MQ=11)                tATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTCTCGTGCTGTCGCGGATCCCCGGTGAAATTGACAGGATTCTCGCCGGGACCCCCCTGCAGGTGTCgcggc                                                                                                                                                                                                                                                                                 >  2:139804/1‑295 (MQ=11)                tATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGAGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACCCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAgcggc                                                                                                                                                                                                                                                                                 >  1:813995/1‑295 (MQ=9)                    aCTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCATGCCGATGCACAGGCGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTCATTTCGTGCTGTCGCCGCTCGCCGGTGAAATTGGCAGGATCCGCGACGGGCACCCGCTGTCGGGGAAGCGGCGt‑tt                                                                                                                                                                                                                                                                            >  2:666269/1‑295 (MQ=11)                        tGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCg                                                                                                                                                                                                                                                                        >  1:292678/1‑295 (MQ=9)                        tGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCTGCAGGCCAGCGAGACAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCg                                                                                                                                                                                                                                                                        <  1:550735/295‑1 (MQ=9)                                                                                                                                          tagtGAGTACGAACGCCAACGACGTAAGCGTGCGCAGGCCGATGCACAGGGGCTGAAAAAGGCCAGAGGCTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAg                                                                                                                                                      <  2:271267/292‑1 (MQ=11)                                                                                                                                            ttGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTc                                                                                                                                                    >  1:200725/1‑295 (MQ=14)                                                                                                                                            ttGAGTACGAACGCCAGCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTc                                                                                                                                                    <  1:346315/295‑1 (MQ=9)                                                                                                                                                        gCCATCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGc                                                                                                                                        >  1:792021/1‑295 (MQ=25)                                                                                                                                                           aTCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGTCACTGGAAAACCGACATGTTGATTTCCTGCAACGGGATATCATCAAAACCATGAACAACGCAGCCGCCCTGGATGAGCTGATAACGTGGATGCTGAGGTAATATATCCAACAGTCAGTCTAACAgacgcc                                                                                                                                     >  2:194212/1‑290 (MQ=11)                                                                                                                                                           aTCGACTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCAAACAGTCAGGTTCACAGACTGc                                                                                                                                     >  2:398998/1‑295 (MQ=255)                                                                                                                                                               aCTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGCACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGCACAGTCAGGCTAACAGGCTGCggta                                                                                                                                 >  1:588498/1‑293 (MQ=255)                                                                                                                                                                cTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACCAAGCAGCCGCGCTGGCTGAACTGATACCGGGGTTGCTTAGTGACTATATCGCACAGGCCGGTTAACCGGCTGCGGcct                                                                                                                                >  2:451012/1‑293 (MQ=11)                                                                                                                                                                cTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCAt                                                                                                                                <  1:412073/295‑1 (MQ=255)                                                                                                                                                                cTTACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCAt                                                                                                                                >  2:236171/1‑295 (MQ=255)                                                                                                                                                                  tACGCGTGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATCTATCGAACAGTCAGGTTAACAGGCTGCGGCAttt                                                                                                                              >  1:871585/1‑295 (MQ=255)                                                                                                                                                                     gcgGGCGAAGGCCGATGCACAGGAGCTGAAAAAGGCCAGAGACGCCGCGGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGACTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGt                                                                                                                           <  2:838088/295‑1 (MQ=14)                                                                                                                                                                       gTGCGCAGGGCGATGCACAGGTGCAGAAAAATGCCAGGGCCTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTGGCGTGCTGTCGCGGATCTCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑GTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTcc                                                                                                                         <  2:583186/295‑1 (MQ=14)                                                                                                                                                                        tGCGCAGGCCGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCGGGTTAACAGGCTGCGGCATTTTGTCcg                                                                                                                        >  1:468653/1‑295 (MQ=255)                                                                                                                                                                             gatctgaggaacaGGGGCTGAAAAATGCCCGAGACTCCGCTGAAGGGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGACTGCGGCATTTTGTCCGCGCCg                                                                                                                   <  2:505692/285‑1 (MQ=14)                                                                                                                                                                                gcGATGCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGGGGAAACCGCATGCGAGACTTGCGTGCGGTGGCGGATCGAAGGGGAAATTGCCAGTATTCACGACGGGAGCCCCCAGACGGTGGAGCGGCGT‑TTGCCGGAACAGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAATCAGCCGCGCTGGATTAACTGATACCGGGGTTGCTGAGTGAATATATCGATCAGGCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGc                                                                                                                <  2:749929/294‑1 (MQ=14)                                                                                                                                                                                  ggggcaaaggggaggaagaaAGCCAGCGACGTCGCGGAAGTGGTGGAGACCACAGCAGGTACTTGCGTGGTGTAGGTGCTCCAAGGAGAAATTGCCAGTAAGCCGGACGGAATCCCCGTGTGGGAGCGGCGGCGT‑TTTCCGGAACTGGAAAAACGAAATATTGATGTCCTGAAACGGGATATCATCAAAGCGAGGAACAAAGCAGCCGCGCTGGATGAGCTGATACCGGGGTTGCTGAGTGAATATATCGAACAGGCAGGTTAACAGACTGCGGCATTTTGTCCGCGCCGGGCtt                                                                                                              <  2:291598/281‑1 (MQ=255)                                                                                                                                                                                   atgCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGACTGCGGCATTTTGTCCGCGCCggagatc                                                                                                             >  1:505692/3‑290 (MQ=255)                                                                                                                                                                                     gCACAGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGc                                                                                                           >  2:583980/1‑295 (MQ=255)                                                                                                                                                                                        caGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACACAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGACGAGTGAATATATCGCACAGTCAGGTTAACAGGCTGCGGCATTTTTTCCTCGCCGGGCTTccacca                                                                                                        >  2:463572/1‑290 (MQ=14)                                                                                                                                                                                        caGGAGCTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCa                                                                                                        <  1:164674/295‑1 (MQ=255)                                                                                                                                                                                          ggAGCTGAAAAATGACAGAGACTCCGCTGAAGTGGGGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCA‑TTTGTCCGCGCCGGGCTTCGCTCACTg                                                                                                     <  1:354721/295‑1 (MQ=255)                                                                                                                                                                                              cTGAAAAATGCCAGAGACTCCTCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGATCAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTc                                                                                                  <  1:178540/295‑1 (MQ=255)                                                                                                                                                                                                 aaaaaTGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATCTATCGAACAGTCAGGTTAACAGGCTGCGTCAGTTTGTCCGCGCCGGGCTTCGCCCCCGGTTCAgg                                                                                               >  2:38510/1‑295 (MQ=255)                                                                                                                                                                                                     cggCCAGGGAGTCCGCTGAGGTGTTGGAAACCGCATTCTGTACTTTCGTGCTGGCGCGGATCGCAGGGGAAATTGCCAGTATTCTCGACGGGATCCCCCTTTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCgg                                                                                           <  2:265519/293‑1 (MQ=255)                                                                                                                                                                                                              ggtcaggtGAAGGGGGGGAAGCCGCAGGGTGGACTGTCGGGCGGGCGCGGAAGGAGGGGGAAAGTGCCATGATTCTCGACGGGAGCCCCCGGTCGGGGCAGCGGCGG‑TTTCCGGAACTGGAAAACCGACAGGTTGATGTACTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCGGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGa                                                                                  <  2:68488/288‑1 (MQ=17)                                                                                                                                                                                                               caccGCGGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGACGCGGATCGCAGGTGAAATGGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTAGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGAc                                                                                 <  1:212143/293‑1 (MQ=255)                                                                                                                                                                                                               cTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGAc                                                                                 <  1:666269/295‑1 (MQ=255)                                                                                                                                                                                                                       aaGTGGTGGAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTg                                                                         <  1:554005/295‑1 (MQ=255)                                                                                                                                                                                                                              ggAAACCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGc                                                                  >  1:622122/1‑295 (MQ=255)                                                                                                                                                                                                                                aaaCCGCATTCTGTACTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAAACGGCTGCGGCATTTTGTCCGCCCCGGCTTTCGCCCACTTTTCCGGACGGCGCCACCGACCGCCGCTGCATGGGCgg                                                                >  2:743329/1‑295 (MQ=17)                                                                                                                                                                                                                                      ccttCTGTACTTGCGGGCTGGCGCGGATCGCAGGGGAAATTTCCAGTATTCGCGACGGGACCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCGGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTa                                                          <  2:12363/293‑1 (MQ=21)                                                                                                                                                                                                                                       aTTTTGGACTTTCTGGCTGGCGCGGATCGCAGGTGAAATTGCCTGGATTTGCGTCGTGATCCCCCGGTGGGTGCCGGGGCGT‑TTTCCGGAACTGTAAAACCGGCATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGGCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTaa                                                         <  2:449512/295‑1 (MQ=18)                                                                                                                                                                                                                                              aCTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTAt                                                  >  1:475446/1‑295 (MQ=255)                                                                                                                                                                                                                                               cTTTCGTGCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCAGTTGAATGGGCGGAGGCTAATTACTAtg                                                 >  1:273187/1‑294 (MQ=255)                                                                                                                                                                                                                                                       ctgtCGCGGATCGCAGGTGAAATTGCCAGTATGCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGaaa                                         <  1:616009/294‑1 (MQ=255)                                                                                                                                                                                                                                                                gttggaGGTGGAATTGCCAGTAGTCTCTACGGGATCCCCCTGTCGGTGCAGCGGGGT‑TGTCCGGAACGGGAAAACCGACATGTTGATTTCCTGAAACGGAATATCATCAAAGCCATGAACAAAGCAGCCGCGCTTGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCa                                <  2:11886/290‑1 (MQ=255)                                                                                                                                                                                                                                                                 tCGCAGGTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCCCCCGAAAGAATCCGCAt                               >  1:76144/1‑295 (MQ=255)                                                                                                                                                                                                                                                                 gtgCAGGGGAAATGGCCAGTAGTCGCGACGGGATCCCCCTGTCGGTGCATCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCAt                               <  2:742034/293‑1 (MQ=255)                                                                                                                                                                                                                                                                  agCAGGGGAATGTGCAAGTCTGCTCGACGGGATCCTCCTGTCGGTGAAGCGTCGT‑TGTCGGGTACGGGAAGACCGAAAAGTTGATTGTCTGAAACGGGGTATCAACAAGGGCATGAAGAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGTGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATa                              <  2:14007/294‑1 (MQ=25)                                                                                                                                                                                                                                                                      ggTGAAATTGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAg                          <  1:726857/295‑1 (MQ=255)                                                                                                                                                                                                                                                                             ttGCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATCCTAATTCATATCCCCCGAAACAATCCGCATACCCGGAAGGgc                   >  1:856819/1‑295 (MQ=255)                                                                                                                                                                                                                                                                               gCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAACGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCAGTGTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCCAACTGCTATCCCCCGAAAGAAACCGCCTACCAGGAAGGgcgg                 >  2:23843/1‑294 (MQ=21)                                                                                                                                                                                                                                                                               gCCAGTATTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAACGAATCCGCATACCAGGAAGGgcgc                 >  1:731024/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                     aTTCTCGACGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGaa           >  1:343405/1‑295 (MQ=255)                                                                                                                                                                                                                                                                                            aCGGGATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTAAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTAAGGACGGCGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTAACATCTACCGACAGATTCCGCATACCAGGCAGGGCGCCGGGTAacaacgc    >  2:80448/1‑291 (MQ=21)                                                                                                                                                                                                                                                                                             cGGGATCCCCCGGGCGAGGCAG‑GGCGTATTTCCGCAACTGGAAAACCGACATGTTGATTTCCAGAAACGGGATATCATCAAAGCCAGGAACAAAGCAGCCGCGCTTGATGAACTGATACCGGAGTTGCTGAGTGAATATATCGAACCGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGGATGGGCGGATGCTAATTACTATCACCCGAAAGAATCCGCATACCAGGAAGAGCGCTGGGAAACACTGcc   <  2:292678/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                              gggATCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCACCCGACAGAACCCGCATACACGGCAGGGAGCCGGGCAACCCGGccc  >  2:303320/1‑295 (MQ=25)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                     GGTAATGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAATTGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCTCCAGCCAGGGACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGCACAGGAACTGAAAAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGTGTTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGCTCCCCCTGTCGGTGCAGCGGCGT‑TTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAAAGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATATCGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCAGGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAATCCGCATACCAGGAAGGGCGCTGGGAAACACTGCCC  >  NZ_LN832404/577880‑578459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑