Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NZ_LN832404	1,982,569	(ATTA)2→1	68.2%	intergenic (‑536/‑117)	flhD ← / → uspC	flagellar transcriptional regulator FlhD/universal stress protein UspC

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_LN832404	= 1982568	24 (0.220)	51 (0.480)	38/550	NT	68.2%	intergenic (‑535/‑121)	flhD/uspC	flagellar transcriptional regulator FlhD/universal stress protein UspC
	?	NZ_LN832404	1982573 =	24 (0.220)					intergenic (‑540/‑116)	flhD/uspC	flagellar transcriptional regulator FlhD/universal stress protein UspC

TTTCAATCTGTTAAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATGCTTGAAGATT  >  NZ_LN832404/1982434‑1982857                                                                                                                                       |                                                                                                                                                                                                                                                                                                  aaagaaaggcaacaatcaacttaatgttAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttaca                                                                                                                                        >  1:1001025‑M1/14‑135 (MQ=255)   agaaaggcaacaatcaaattaatgTTAATTAAATGTTTTACATATATTAACCACTTGGTTAATGCGGAGATTCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacacc                                                                                                                                      <  1:768341‑M1/279‑158 (MQ=255)        gtgaacaatcaactTAATGTTAAAGAAAAGTTTTACATATATTAACCACTTGGTTAATGCGGAGAATCAGTACACTATTTGCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaa                                                                                                                                 <  2:536020‑M1/284‑163 (MQ=255)         gcaacaatcaacTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaatacggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaag                                                                                                                                >  1:673‑M1/6‑127 (MQ=255)         gcaacaatcaacTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatcttcttgtcgctgttgcggttacaccggaaag                                                                                                                                >  1:924506‑M1/6‑127 (MQ=255)         gcaacaatcaacTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaag                                                                                                                                <  2:971607‑M1/285‑164 (MQ=255)           aacaatcaACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtc                                                                                                                              >  1:573921‑M1/4‑125 (MQ=255)            acaaTCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGaaa                                                                                                                             <  2:50696/288‑1 (MQ=255)              aaTCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagc                                                                                                                           <  1:517955‑M1/290‑169 (MQ=255)              aaTCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtaagc                                                                                                                           >  1:689633‑M1/1‑122 (MQ=255)              aaTCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagatatcgcaatattcttgtcgatgttgcggttaacccggacagtccgc                                                                                                                           >  2:830077‑M1/1‑122 (MQ=255)               aTCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatggcaatattcttgtagctgttgcggttacaccggaacgtcagga                                                                                                                          >  2:512015‑M1/1‑121 (MQ=255)                             aatgAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTTTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTCATGTCACCTGCAGTTCACAGAATAAGGAAACGTTTCTGCGCTATACAAATCTTATTTTCACTGTTGCTGTTCACCCGGACCGTCAGCACATGCTAGCa                                                                                                            >  2:848248/1‑290 (MQ=255)                             aatgAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACATGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAAGATTCTTGTCGCTGTTGCGGTGTCAACGGAAAGTGAGCAActgagtgca                                                                                                            >  2:981663/1‑284 (MQ=255)                                 aaaTGGTGTAAAGATATTAAACAACTGTGTAATGCGTAGAATCAGTACACTATTTTCTTATAATTACAGTTGACATTATATGATCACCTGTAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATGTACCTGTTTGACCGAGTTGGGATTGCGTCGGTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAg                                                                                                        <  2:889991/290‑1 (MQ=255)                                    tGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatc                                                                                                     <  1:108827‑M1/290‑191 (MQ=255)                                        ttACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacacctgaacgtcaacaactgcttgccaaagcggtttatatc                                                                                                 >  2:572447‑M1/1‑96 (MQ=255)                                        ttACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaacgcggtatctatc                                                                                                 >  1:419797‑M1/1‑96 (MQ=255)                                         tACATATATTAACCACTTGTTTAATGCGGAGAATTAGTACACTATTTTCTTATAATTACATTTGAAAGTATATGATTACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGtatct                                                                                                <  2:202899/290‑1 (MQ=255)                                          aCATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtagctgttgcggttccaccggaaagtcagcaactgcgggccaaagcggtatctatcgc                                                                                               >  2:650770‑M1/1‑94 (MQ=255)                                               tatTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagcctaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggcacaggttatgagctatagcaatattcttgtcactgttgcggttacaccggaaagtccgcaactgctggcacaagctgtatctatcgccagtc                                                                                          >  2:516209‑M1/1‑89 (MQ=255)                                               tatTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacacc                                                                                                                                      >  1:400956‑M1/1‑89 (MQ=255)                                               tatTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacacc                                                                                                                                      <  2:400956‑M1/246‑158 (MQ=255)                                                atTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggcc                                                                                         <  1:168267‑M1/290‑203 (MQ=255)                                                atTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAACGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTAAACCGGAAAGTCAGCAACTGCTGGCAAAAGCggagacttcgaccg                                                                                         >  2:576383/1‑278 (MQ=255)                                                  tAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaacgtcagaaactgctggcaaaagcggtatctatcgcaaggccag                                                                                       >  1:587390‑M1/1‑86 (MQ=255)                                                     ccACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaa                                                                                    >  1:103652‑M1/1‑83 (MQ=255)                                                       aCTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaag                                                                                  >  2:271032‑M1/1‑81 (MQ=255)                                                         ttGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaaggg                                                                                >  1:755264‑M1/1‑79 (MQ=255)                                                         ttGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagacatattcttgtcgctgttgcggttacaccggaaagtcagcaaaggatggacaacgcggtacatatcgccaaggcagaacacgt                                                                                >  2:541611‑M1/1‑79 (MQ=255)                                                           gTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAg                                                                              >  1:125757/1‑290 (MQ=255)                                                               aaTGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcaaagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagaaactgctggcaaaagcggtatctatcgcaaggccaggaaggggcaacag                                                                          >  1:276885‑M1/1‑73 (MQ=255)                                                                 ttaggagaATCAGTAAAATATTTTCTTATAATGACATTTGAAATTATATGATAACCGGGAGGGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatca                                                                        <  2:911244‑M1/287‑220 (MQ=255)                                                                  gCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcag                                                                       >  1:201584‑M1/1‑70 (MQ=255)                                                                     gagaATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggaataaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgcttttgcggttacacaggaacgtcagcaactactggcaaaagcggtatctattgccaggccagacaagggccacatcagttc                                                                    >  2:692515‑M1/1‑67 (MQ=255)                                                                      agaATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCTCTTTTGCGGTTACACCGGAAAGTCAGCAAATGCTGGCAACAGCGGTATCTATCGCCAGGCCCGTAACGGGCCACATCAg                                                                   >  1:756321/1‑290 (MQ=255)                                                                       taatatgTACATTATTTTGTTATAAGTGCATTTGAAATTATAAGTTCCCTGGGAGTG‑‑TG‑‑TGAGTATATGTACTAATAAACATAGTAATATTTACGTTTTTGACACGGTGGGTATTGCGTCGTTTATCCATGTGGAGTAAAGCTGTAATGTCCCCTTTAGTTCACAGAATAAGGAACAGGATATGAGCTATAGCACTATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCTAGGCCAGTAAAGGGAAACATCAGTTTaa                                                              <  2:261053/284‑1 (MQ=255)                                                                       gaATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCGCCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGt                                                                  >  1:126524/1‑290 (MQ=255)                                                                       gaATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGACGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGATAGTCAGCAACTGCTGGCCAAAGCGGTATCTATTGCCCGGCCAGTAAAGGGACACATCCGt                                                                  >  2:884614/1‑290 (MQ=255)                                                                       gaATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCACGGCCCGTAAAGGGACCAATCAgg                                                                  >  2:1038576/1‑289 (MQ=255)                                                                        aaTCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgcaaggccagtaaagggacacatcagtttaat                                                                 >  1:678867‑M1/1‑64 (MQ=255)                                                                         atacttaaagagttttcttataagttaaTTGTCAATTATATGATGACTGGGAGTGATTGATTAtctttacttatagtcagactaatatttacctgtttgaccgagttgggattgtgtagtgtctccattaggagtaaagctttaatgtaacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaatt                                                                <  2:212819‑M1/278‑228 (MQ=255)                                                                           cAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagaccaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattac                                                              <  1:682505‑M1/290‑230 (MQ=255)                                                                                   tATTTTCTTATAATTTCATTTGAAATTATATGGTAACCTGGAGTGATTGATTAgctttactaataattagactaatagttacctgtttgaccgagttgggattgcggcgttactccattaggagtaatgctttaatttcacctgaatttcacagaataaggaacaggttatgagctatagcaatattcttttcgctgttgcggttacaccggaaagtcagcaactgctgtcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgctt                                                      <  2:980998‑M1/290‑238 (MQ=255)                                                                                    aTTTTCTTATAATTACATTTGAAATTATAGGAGCACCGTGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCg                                                     <  2:34856/290‑1 (MQ=255)                                                                                           ttttaTTTCATTTGAAATTATATGATAACCGTGAGTTATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCGGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGa                                              <  2:761383/286‑1 (MQ=255)                                                                                              aaTTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGAccc                                           <  1:857931/290‑1 (MQ=255)                                                                                                ttACATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaat                                         >  1:281574‑M1/1‑40 (MQ=255)                                                                                                 tACATTTGAAATTATATGATCACCGGGAGGGATTGATTAtttttactaataatcagagtaatatttacctgtttgaccgagttgggattgcgtcgtttttccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatg                                        <  2:766676‑M1/290‑252 (MQ=255)                                                                                                  aCATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggagcaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgt                                       >  1:846272‑M1/1‑38 (MQ=255)                                                                                                  aCATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaccaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctgtccaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacacggaactgt                                       >  1:846242‑M1/1‑38 (MQ=255)                                                                                                  aCATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgt                                       >  1:4307‑M1/1‑38 (MQ=255)                                                                                                  aCATTTGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccagccaagtaaagtgacacaccagtttaattactctccattccgacccgcaaatgt                                       >  2:1030715‑M1/1‑38 (MQ=255)                                                                                                      ttGAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaa                                   <  2:50789‑M1/290‑257 (MQ=255)                                                                                                        gAAATTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaactgtacaatc                                 >  1:290746‑M1/1‑32 (MQ=255)                                                                                                          aaTTATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatcaa                               >  1:921741‑M1/1‑30 (MQ=255)                                                                                                           aTTATATGATCACCGGGAGTTATTTATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttttccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatcaat                              <  1:735075‑M1/290‑262 (MQ=255)                                                                                                            ttATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatcaatt                             <  1:435980‑M1/290‑263 (MQ=255)                                                                                                            ttATATGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttcgccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatcaatt                             <  1:960914‑M1/290‑263 (MQ=255)                                                                                                             tataTGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCATACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTAcaatca                            <  2:89188/290‑1 (MQ=255)                                                                                                              atagtaTCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTGCTCCATGAGGAGTAAAGCTTTAATGTAACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTAcaatcaa                           <  1:923943/285‑1 (MQ=255)                                                                                                                 tGATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaacgggacacatcagtttaattcctctcgcttccgacccggcactttacaatacattagcct                        >  2:516884‑M1/1‑23 (MQ=255)                                                                                                                  gttcaccggtgggtattgattatctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatcaattagctgc                       <  2:488173‑M1/276‑269 (MQ=255)                                                                                                                  gATCACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatccattagctgc                       >  1:309188‑M1/1‑22 (MQ=255)                                                                                                                    tCACCGGGAGTGATTGATTAtatttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatcaattagctgcgc                     <  1:684705‑M1/290‑271 (MQ=255)                                                                                                                     cACCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatcaattagctgcgcc                    <  1:291210‑M1/290‑272 (MQ=255)                                                                                                                      aCCGGGGTTGATTGATTAtctttactaataatcatactaataattaccgggttgaccgagttgtgattgcgtcgtttatccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatcaattagctgcgccg                   <  2:388401‑M1/290‑273 (MQ=255)                                                                                                                      aCCGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatcaattagctgcgccg                   >  1:494261‑M1/1‑18 (MQ=255)                                                                                                                       ccGGGAGTGATTGATTAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaacgtcagcaactgctggcaaaagcggtatctatcgcaaggccagcaacgggacacctcagtttaattactaccgcttccgacccggaaatgtacaatcaattagctgacccga                  >  2:205945‑M1/1‑17 (MQ=255)                                                                                                                       ccGGGAGTGATGGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTgcg                  <  1:76982/290‑1 (MQ=255)                                                                                                                        cgtgAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTgcgc                 <  1:408042/287‑1 (MQ=255)                                                                                                                         gggAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTAACGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTAACATCAAATTGCTGcccc                >  2:351391/1‑290 (MQ=255)                                                                                                                             gggagtgattgatTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAAAAGGTTATGAGCTATAGCAATATTCTTGTAGCTGTTGCGGATACACCGGACACACTGCACCTTCTGGCCAAACCGGTATCTATCGCCAGGCACGCAAAGGCAAAACACAGTTTAATTACTCCCTCTTCCGACCCGGAAACTGACACTCCATTATCTgcccacaat            >  2:883221/6‑283 (MQ=255)                                                                                                                               gattgattAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATGCt          >  1:122470/1‑290 (MQ=255)                                                                                                                               gattgattAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATGCt          >  1:696926/1‑290 (MQ=255)                                                                                                                                attgattAATATACTTTAAGAATAATCATACTAGAATTGACTTGTTTGACCGGTTTGGGTTGTGGTGGTTTCTCCATTAGGATGAAAGCTTTAATTTAACCTGAAGTTCAAAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATGCtt         <  2:1063024/290‑1 (MQ=255)                                                                                                                                     ttAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctctcgccaggccagtaaagggacacatcagttgacttactctcgcttccgacccggaaatgtacaatcaattagctgcccagaggcttgaagcttta    >  2:828305‑M1/1‑3 (MQ=255)                                                                                                                                      tAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcttcgtttctccattaggagtaaagctttgatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatcaattagctgcgccgatgcttgaagatttac   <  1:333131‑M1/290‑289 (MQ=255)                                                                                                                                      tAtctttactaataatcagactaatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaacgcggtcactaacgcccggccaggaaagggaaccatcagtttaattaatctcgcttcagaacccgaaatggacaaacaattagctgagcatatgcttgacgatttac   >  2:807697‑M1/1‑2 (MQ=255)                                                                                                                                       atctttactaataatcagaataatatttacctgtttgaccgagttgggattgcgtcgtttctccattaggagtaaagctttaatgtcacctgaagttcacagaataaggaacaggttatgagctatagcaatattcttgtcgctgttgcggttacaccggaaagtcagcaactgctggcaaaagcggtatctatcgccaggccagtaaagggacacatcagtttaattactctcgcttccgacccggaaatgtacaatcaattagctgcgccgatgcttgaagatttacg  >  1:706716‑M1/1‑1 (MQ=255)                                                                                                                                       |                                                                                                                                                                                                                                                                                                  TTTCAATCTGTTAAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTGAAGTTCACAGAATAAGGAACAGGTTATGAGCTATAGCAATATTCTTGTCGCTGTTGCGGTTACACCGGAAAGTCAGCAACTGCTGGCAAAAGCGGTATCTATCGCCAGGCCAGTAAAGGGACACATCAGTTTAATTACTCTCGCTTCCGACCCGGAAATGTACAATCAATTAGCTGCGCCGATGCTTGAAGATT  >  NZ_LN832404/1982434‑1982857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.