Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 2,407,278 A→G 74.8% L71S (TTG→TCG)  EK12AG100_RS12000 ← NADH‑quinone oxidoreductase subunit NuoB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324042,407,2780AG74.8% 187.3 / 70.8 103L71S (TTG→TCG) EK12AG100_RS12000NADH‑quinone oxidoreductase subunit NuoB
Reads supporting (aligned to +/- strand):  ref base A (10/16);  new base G (33/44);  total (43/60)
Fisher's exact test for biased strand distribution p-value = 8.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 109 total aligned reads displayed.

GCATGTACGCTTCAGGACGCGGCGGGCAGCCCGGGATATACACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCGATACTGTTCGTTTCCGGGTTCATACGCTCGCGGCGTGAACG  >  NZ_LN832404/2406991‑2407564
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                               
gCATGTACGCTTCAGGACGCGGCGGGCAGCCCGGGATATACACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGACTAACCGGTGCCACTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCCGCGAAGCACGCAATACTTCTGCGCCAACACGCGCCACGTCATGCACCGCGGTAAACgaa                                                                                                                                                                                                                                                                                               >  1:396345/1‑290 (MQ=255)
      aCGCTTCAGGACGCGGCGGGCAGCCCGGGATATACACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCCATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAACGtacac                                                                                                                                                                                                                                                                                         >  2:654561/1‑286 (MQ=255)
        gCTTCAGGACGCGGCGGGCAGCCCGGGATATACACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCAt                                                                                                                                                                                                                                                                                       >  1:115100/1‑290 (MQ=255)
         cTTCAGGACGCGGCGGGCAGCCCGGGATATACACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCAtc                                                                                                                                                                                                                                                                                      <  1:105069/290‑1 (MQ=255)
             aGGACGCGGCGGGCAGCCCGGGATATACACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCaa                                                                                                                                                                                                                                                                                  >  1:624157/1‑290 (MQ=255)
              gggggcggcgGGCAGCCCGGGATATACACATCAACCGGTATGAAATTATCGACGCCCTGCAAAACGGAATAAATATCGTACAGAACACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATGTTGGTTCCAGCATCTGGTCATACAGACGATGAATAACCGGTGCCATTGTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAAc                                                                                                                                                                                                                                                                                 <  2:608427/286‑1 (MQ=255)
              ggaaccggcggGCAGCCCGTGATATACACAGCACCCCGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCAGTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAAc                                                                                                                                                                                                                                                                                 <  2:215608/285‑1 (MQ=255)
                     gcggGCAGCCCGGGATATACACATCAACCGGGATGAATTTATCGACGCCCTGCAAAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCAACCATTGAGATAACCCAGTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAAcag                                                                                                                                                                                                                                                                          <  2:241934/290‑1 (MQ=255)
                            gCCCGGGATATACACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGaaa                                                                                                                                                                                                                                                                   <  1:635818/290‑1 (MQ=255)
                             cccGGGATATACACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTCACAGCAGGAAAg                                                                                                                                                                                                                                                                  >  1:40859/1‑290 (MQ=255)
                                  gcactaCACATCAAACGGGTACAAGTTCTCGACGCCTTGCACAACTGAATAAATAGCGTACATAGCACCAGCGTTGGCACAGGCACCCATTGAGACAACCCATTTTGGTTCCAGCATCTGTGCATACGGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACGTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGa                                                                                                                                                                                                                                                             <  2:507872/286‑1 (MQ=255)
                                    tataCACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCTAAGCACGCAATACTTCTGCGCACAAACGCGCCCCGTCATGCACCGCGGTAAACGCAGTCACCATCACAACGAACCAGCAAGaaaagacaaat                                                                                                                                                                                                                                                           >  2:1038857/1‑282 (MQ=255)
                                        cacaTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCGTTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTtat                                                                                                                                                                                                                                                       <  1:321272/290‑1 (MQ=255)
                                        cacaTCAACCGGGATGAATTTATCGACGCACTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTtat                                                                                                                                                                                                                                                       >  1:776685/1‑290 (MQ=255)
                                        caactCAACCGTGATGAATTTATCTACGCCCTGCACACAGGAATAAATATCGTCAAGACCACCAGAGTTGGCACAGGCACCCATTGATATAACCCATTTTGGTTCCAGCATCGGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTtat                                                                                                                                                                                                                                                       <  2:1026099/286‑1 (MQ=255)
                                          caTCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATAc                                                                                                                                                                                                                                                     <  1:345894/290‑1 (MQ=255)
                                                 cGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCaaa                                                                                                                                                                                                                                              <  2:624157/290‑1 (MQ=255)
                                                  gggATGAATGTATCGACGCACTGCACAACTGAATAAAGATCGTACATACCACCAGTGTTGGCACAGTCAACCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCAATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAAt                                                                                                                                                                                                                                             <  1:11730/290‑1 (MQ=255)
                                                       gAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCCATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCCCAACGCAACAGCAGGAAAGACCGACGTTATACGGCCCAACTGAGt                                                                                                                                                                                                                                        >  2:370033/1‑290 (MQ=255)
                                                       gAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGt                                                                                                                                                                                                                                        >  1:967960/1‑290 (MQ=255)
                                                        aaTTTAACGACGCCCGGAACAACGGACAAAACATCGTACATACCCCCAGAGTTGGAACAGGCACCCATTGGGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCAGTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGtt                                                                                                                                                                                                                                       <  2:331637/290‑1 (MQ=255)
                                                          tttATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGtttt                                                                                                                                                                                                                                     >  1:61217/1‑290 (MQ=255)
                                                           ttctCGACGCCCTGCACAACGGAATAAATATCGTACCTACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGttttt                                                                                                                                                                                                                                    <  2:66073/287‑1 (MQ=255)
                                                           tgtatgacgtcgcacacaACGAAATAAAAATCTAACATACCACCTGGTATGGCACAGGCACCCATTGAGATAACCCAGTTTGGGTCCAGCGTCTGGGCATACAGACGCTGAATAACCGTTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACGTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGttttt                                                                                                                                                                                                                                    <  2:253802/276‑1 (MQ=255)
                                                             aTCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTAc                                                                                                                                                                                                                                  <  2:529284/290‑1 (MQ=255)
                                                                gACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCCATACTTCTGCGCCAAAACGCGCCACGTCATGCCCCGCGGTAAACAAAGTCACCATCTCAAAGTAACAGCCGGCACGACCGAAGTTATACGGCCCAATTGACTTTTCACGAc                                                                                                                                                                                                                               >  2:335786/1‑290 (MQ=255)
                                                                gACGCCCTGCACAACGGAATAAATATCGGACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCAGTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGAc                                                                                                                                                                                                                               <  1:920556/290‑1 (MQ=255)
                                                                             aCGGAATAAAGACCGTACAGACCACCAGGGTTGGCACAGGCACCCATTGGGATAACCCATTTTTGTTCCAGCATCTGGTCAGACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGTGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCAt                                                                                                                                                                                                                  <  2:699421/290‑1 (MQ=255)
                                                                                    aaataTCTAACATCCCACCAGAGTTTGCACAGGCACCCATGTAGATAACCCATTTTGGTTTCAGCATCTGGTCATACAGACGCTGAATAACCTGGGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTg                                                                                                                                                                                                           <  2:914942/290‑1 (MQ=255)
                                                                                    aaataTCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCCCCGCGGTAAACAAAGTCCCCATCCCACCGTAACAGCCGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTAAGACACCAGTTAACCATgcacttc                                                                                                                                                                                                           >  2:217459/1‑284 (MQ=255)
                                                                                     aataTCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGa                                                                                                                                                                                                          >  1:774329/1‑290 (MQ=255)
                                                                                       tcacGTACATCCCACCAACGTTGGCACGGACACCGATTGGGATAACCGATTTTGGTTCCGGCATCTGGTCATACGGACGCTGAATGACCGGTGCCATTTTGGTAAAGCAGTTTCCTGCAACCCCCAGCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGc                                                                                                                                                                                                        <  2:414445/287‑1 (MQ=255)
                                                                                       tatCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGGATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGc                                                                                                                                                                                                        <  1:332378/290‑1 (MQ=255)
                                                                                        atCGTACATACCACCAGAGTTGGCACAGGAACCCATTGAGATAACCCAGTTTGGGTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCt                                                                                                                                                                                                       <  2:574215/290‑1 (MQ=255)
                                                                                            gactcaccaccCGAGTTGGCACAGGCAGCCATTGAGATAACCCATTTTGGTGCCAGCATCTGGTCATACAGACGCTGAATAACCGGGGCCATTGTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCGTACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGcc                                                                                                                                                                                                   <  2:802434/285‑1 (MQ=255)
                                                                                               aTACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCAt                                                                                                                                                                                                <  1:1055862/290‑1 (MQ=255)
                                                                                                   caccaGAGTTGGCACAGGCAACCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAac                                                                                                                                                                                            <  1:839559/290‑1 (MQ=255)
                                                                                                       agagTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGt                                                                                                                                                                                        <  1:82364/290‑1 (MQ=255)
                                                                                                              gCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGtt                                                                                                                                                                                 <  1:944765/290‑1 (MQ=255)
                                                                                                                    gCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGATCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGAAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAActtc                                                                                                                                                                           <  1:328567/290‑1 (MQ=255)
                                                                                                                         catggaggaaaCCAAGTTTGGTTCCAGCAGCTGGTAATACAGACGCGGAATAACCGGTGCCAGTGTGGTAAAGCAGGTTCCTGCAACCACCAGAAGGTCAGCCTGACGCGGCGAAGCACGAAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCt                                                                                                                                                                      <  2:105471/281‑1 (MQ=255)
                                                                                                                          aTTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGCTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCATGACCATAACCACATTTTTGTTAActtattcatc                                                                                                                                                                     >  2:1027596/1‑286 (MQ=255)
                                                                                                                           ttGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGGAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTcc                                                                                                                                                                    <  1:187114/290‑1 (MQ=255)
                                                                                                                              agaTAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCaga                                                                                                                                                                 >  1:97331/1‑290 (MQ=255)
                                                                                                                               gaTAACCCATGTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCagag                                                                                                                                                                <  1:914018/290‑1 (MQ=255)
                                                                                                                                aTAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAgg                                                                                                                                                               >  1:454228/1‑290 (MQ=255)
                                                                                                                                   acacgttgtGGTTCCAGCCTGTGGACCTACAGACCCTTACTAACAGGTGCCACTGTGGTAAATCAGGTTCCTGCCATCACCATCACGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTc                                                                                                                                                            <  2:143626/282‑1 (MQ=255)
                                                                                                                                       agtctGGTGCCTGCATGTGGTTATACAGGTGCCGAATAACCGCTGCCATTTTGGGAAAGCAGGTTCCGTCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGtt                                                                                                                                                        <  2:556436/286‑1 (MQ=255)
                                                                                                                                       agcttGGCTCCAGAAACTGGGCAAACAGTAGGTGAATACGCGTGGCCAGTCTGGTAAAGCAGGTACCTGCAACCACCATAGGGACAGCCTGACGCTGCGAAGCACGCAATACGACTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGGCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGGTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGGTTTTGTTAACTTCTTGCTCCAGAGGGTCGGtt                                                                                                                                                        <  2:249360/287‑1 (MQ=255)
                                                                                                                                       aTTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAACTTGAGTCTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACCAGTATTTGTTAACTTCTTGCTCTAGAGGGTAgggt                                                                                                                                                        >  2:682305/1‑288 (MQ=255)
                                                                                                                                        ttttGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCCACAGAGGGTCGGTTa                                                                                                                                                       >  1:1041466/1‑290 (MQ=255)
                                                                                                                                            ggTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGAt                                                                                                                                                   <  1:177376/290‑1 (MQ=255)
                                                                                                                                               tCCATCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGAtctc                                                                                                                                                <  1:287611/290‑1 (MQ=255)
                                                                                                                                                 cAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCt                                                                                                                                              <  1:588301/290‑1 (MQ=255)
                                                                                                                                                  aGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTg                                                                                                                                             <  1:474916/290‑1 (MQ=255)
                                                                                                                                                    cATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCt                                                                                                                                           <  1:971725/290‑1 (MQ=255)
                                                                                                                                                     aTCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAACCGAAGTCACCATCTCACCGTAACAGCAGGAAAGAACGAAGTTATACGGCCAACTTGAGTTTTTACGACCCCAGTTAACCATGTCATTGACCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCCGAGGGTCGCTTACGATCCCCTgact                                                                                                                                          >  2:781966/1‑287 (MQ=255)
                                                                                                                                                      gttGGTAATACGAACGCGGAATAACCGTGACCATGTTGGTAAAGCAGGTTCCTGCAAACACCATCAGGTCAGCCTGACGCGGCGAAGCACGAAATACTATTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCGCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCttt                                                                                                                                         <  2:912028/288‑1 (MQ=255)
                                                                                                                                                       cTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGGTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCtttt                                                                                                                                        >  1:759011/1‑290 (MQ=255)
                                                                                                                                                            cATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAgg                                                                                                                                   >  1:855333/1‑290 (MQ=255)
                                                                                                                                                                  gACGCGGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCGGACGCGGCGAAGCACGCAATCCTTCTGCTCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTaa                                                                                                                             <  1:983827/290‑1 (MQ=255)
                                                                                                                                                                   aCGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATCGCCTTGAGCTTGCCCATACACCAGTTTTTGTTAACTTCTTTCTCCAGAGGGTCGGTTACGATCCTCTGCTATTGCAGGGGTTAAc                                                                                                                            >  2:334003/1‑290 (MQ=255)
                                                                                                                                                                      cTGAATAACCGGTGCCAGTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGt                                                                                                                         <  1:654561/290‑1 (MQ=255)
                                                                                                                                                                        gAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGTTAACGGTCg                                                                                                                       >  1:498647/1‑290 (MQ=255)
                                                                                                                                                                           gaaacGGTGCCATTTTTGTAACGCAGGTTCCTGCAACAACCATCAGGTCAGCCGGACGCGGGGAAGAACGCAATACTGCGGCGCCAAAACGCGCAACGTCATGCACCGCGGTAAACGAAGTCACCAGCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTtc                                                                                                                    <  2:387806/286‑1 (MQ=255)
                                                                                                                                                                            aaCCGGTGCCAGTTGGGTAAAGCAGGTTCCTGCAACCACCACAAGGGAAGCCGGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCAACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTtct                                                                                                                   <  2:1046523/290‑1 (MQ=255)
                                                                                                                                                                                 gTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGATAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCACACTGAGTTTTTACGACCCCAGTTCACCATGTCCTTGAGCTTGACCATAAACACGTTTTTGTCAACTTCTTGCTCCATACGGTCGTTTACGATCTCCTTGTTTCGCAGGGGTGAACGGTTGTtttccacg                                                                                                              >  2:1007321/1‑286 (MQ=255)
                                                                                                                                                                                       atttGGTAAAGCGAGTTCCGGCAACCACCATCAGGCCAGCCTGCGCCGGCGAAGCACGCAAGACTTCTGCGCCAAAACGCGCCACGTCATGAACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGa                                                                                                       <  2:170776/289‑1 (MQ=255)
                                                                                                                                                                                        tttGGTAAAGCAGGTTCCTGCAACCACCAGCAGGGCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGAt                                                                                                      <  2:80268/290‑1 (MQ=255)
                                                                                                                                                                                           ggTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTa                                                                                                   <  1:246575/290‑1 (MQ=255)
                                                                                                                                                                                            tgaaaTCAGGGTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTAt                                                                                                  <  1:579125/288‑1 (MQ=255)
                                                                                                                                                                                            gTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTTCTCCTGCGGGTCGGTTACGATCTCCTCGTTTTTCAGGGGGTCACGGTCGTTTCACCCTTTGGGATCTAt                                                                                                  >  2:773530/1‑290 (MQ=255)
                                                                                                                                                                                            gTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTAt                                                                                                  <  1:161241/290‑1 (MQ=255)
                                                                                                                                                                                                   agtgtCCTTCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTGCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTg                                                                                           <  2:40859/286‑1 (MQ=255)
                                                                                                                                                                                                   aGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTg                                                                                           <  1:627420/290‑1 (MQ=255)
                                                                                                                                                                                                     gTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAAACATGTCATATAGCTTGCCCATAAAAAAGTTTTTGTTAACTGTTTGCTACAGGTGTACGATTAAGATATCTTGTTTTAGAAGGGGGTAACGGTAGTGTTGACGGTTGGGGGCTATGCgggggtg                                                                                         >  2:365406/1‑286 (MQ=255)
                                                                                                                                                                                                       tCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCg                                                                                       <  1:378325/290‑1 (MQ=255)
                                                                                                                                                                                                          tGCAAGCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCAGCGCCAAAACGCGCCAAGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGtat                                                                                    <  2:793344/290‑1 (MQ=255)
                                                                                                                                                                                                             aaCCACCATCAGGTCCGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCCAACCGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAAt                                                                                 <  1:196016/290‑1 (MQ=255)
                                                                                                                                                                                                               ccaccaTCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGTATCTATGCGCGTGAGCGTATCATcc                                                                               >  1:882073/1‑290 (MQ=255)
                                                                                                                                                                                                                  ccaTCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATc                                                                            >  1:756269/1‑290 (MQ=255)
                                                                                                                                                                                                                    aTCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCAACGTTGGGCTCTATGCGGGTGAGCGTATAATCAATCtt                                                                          >  1:645071/1‑290 (MQ=255)
                                                                                                                                                                                                                              ccTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCCTGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTCCGATCTCCTGCTTTTGCCGGGGGTACCGGTCGTTCTCACCGTTTGGATCTATGCGGGTGACCGTATAATCCATCTTAATGCCTccg                                                                >  2:972686/1‑288 (MQ=255)
                                                                                                                                                                                                                              ccTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATCATCCATCTTAATGCCTcgc                                                                >  1:932269/1‑290 (MQ=255)
                                                                                                                                                                                                                                   cgcgGCGCAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGATa                                                           >  1:618272/1‑290 (MQ=255)
                                                                                                                                                                                                                                         gAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGGTCTATGCTGGTGGGCGTGTGATCCATCTTAATGCCTCACGGTTGGCGGTg                                                     >  2:250347/1‑290 (MQ=255)
                                                                                                                                                                                                                                                  aaTACTTCTGCGCCAAAACGCGCCACGTCAGGCACCTCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGc                                            <  2:1030397/290‑1 (MQ=255)
                                                                                                                                                                                                                                                   aTACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCg                                           >  1:664328/1‑290 (MQ=255)
                                                                                                                                                                                                                                                     aCTTCTGCGCCAAAACGCGCCACGTCATGCAACGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCGAt                                         <  1:335786/290‑1 (MQ=255)
                                                                                                                                                                                                                                                        tCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTACCGGTCGATCTCACCGTTGGGATCTATGCGGGTGAGGTTATAATCCATCTTAATGCCTACAGTTTAGCGGTGACGATACGCTATAcg                                      >  2:591108/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            cgcAAAAACGCGCCACGTCAGGCACCGCGGTAAACAAAGGCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTGTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGGTTTTGTTAACTTCTGGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCGATACTGTTc                                  <  2:762388/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                     gcgcCACGTCATGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCGATACTGTTCGTTTCCggg                         >  1:971207/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                               aTGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGATCTTGCCGGGGGTAACGGTCGTTCTAACCTTTGGGATCTCTGCGGATACGCGTTTCATCCATCTTCATTCCTCGAGGTTAGCGTTGCCGATTACGGATACTGTTCTTCTCCAGGTTcccactct               >  2:3504/1‑283 (MQ=255)
                                                                                                                                                                                                                                                                                tGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGCTCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTCGCGTTGATGATTTGCGATACTGTTCGTTTCCGGGttactagtctc              >  2:79747/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                                tGCACCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCGATACTGTTCGTTTCCGGGTTCATACGCTc              <  2:97331/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   aCCGCGGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCGATACTGTTCGTTTCCGGGTTCATACGCTcgcg           >  2:598109/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                      gcgGTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTCGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGTATAGCGATCATGTTCGTTTCAGGGTTCATGCGCGCtcggca        >  2:121856/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                         gTAAACGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCGATACTGTTCGTTTCCGGGTTCATACGCTCGCGGCGTGa     >  1:1061714/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                           aaaCAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTATCACCGTTGGGATCTATGCGGGTGAGCGTATCATCCATATTAATGCCTCGCGGTTAGCGTTGACGATTAGCGATACTGTTCGTTTCCGGGTGCATACGCTCGCGGCGTGAAc   >  2:595955/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                            aaCGAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCGATACTGTTCGTTTCCGGGTTCATACGCTAGCGGCGTGAAcg  >  1:562561/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                               
GCATGTACGCTTCAGGACGCGGCGGGCAGCCCGGGATATACACATCAACCGGGATGAATTTATCGACGCCCTGCACAACGGAATAAATATCGTACATACCACCAGAGTTGGCACAGGCACCCATTGAGATAACCCATTTTGGTTCCAGCATCTGGTCATACAGACGCTGAATAACCGGTGCCATTTTGGTAAAGCAGGTTCCTGCAACCACCATCAGGTCAGCCTGACGCGGCGAAGCACGCAATACTTCTGCGCCAAAACGCGCCACGTCATGCACCGCGGTAAACAAAGTCACCATCTCAACGTAACAGCAGGAAAGACCGAAGTTATACGGCCAAATTGAGTTTTTACGACCCCAGTTAACCATGTCATTGAGCTTGCCCATAAACACGTTTTTGTTAACTTCTTGCTCCAGAGGGTCGGTTACGATCTCCTGCTTTTGCAGGGGGTAACGGTCGTTCTCACCGTTGGGATCTATGCGGGTGAGCGTATAATCCATCTTAATGCCTCGCGGTTAGCGTTGACGATTAGCGATACTGTTCGTTTCCGGGTTCATACGCTCGCGGCGTGAACG  >  NZ_LN832404/2406991‑2407564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: