Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 2,549,680 A→T 78.9% E44D (GAA→GAT murP → PTS N‑acetylmuramic acid transporter subunit IIBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324042,549,6800AT78.9% 160.6 / 39.9 71E44D (GAA→GATmurPPTS N‑acetylmuramic acid transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (9/6);  new base T (31/25);  total (40/31)
Fisher's exact test for biased strand distribution p-value = 7.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

GCAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCG  >  NZ_LN832404/2549396‑2549962
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                          
gcAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGACATACCGCCGGTTGTTGTAACTGTATGACGCGCCTGCGTCGTGTTGTACATGACCGGTCACTGGTTGACACCaa                                                                                                                                                                                                                                                                                       >  2:110204/1‑290 (MQ=255)
 caatccAAGCCGAAGCTGCGTTATTTGCGTGCTATCGCAACTGTAAAACGGCCATTGTTATGGTGCTTAAAAATCTCGATGCCGCAGAATCTAAAAAACTCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTCGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCATTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAat                                                                                                                                                                                                                                                                                      <  2:823929/285‑1 (MQ=255)
   gagCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAatat                                                                                                                                                                                                                                                                                    <  1:967736/290‑1 (MQ=255)
         gcggaagcggGTTTAATGGCTTACGAGCGAAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAAc                                                                                                                                                                                                                                                                              <  2:46315/290‑1 (MQ=255)
              agcggCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTCTTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAACGAGATCAGCAGTGAACTTCTGAACAACATTCTTACCCGTGTCGGCGGCCCGGGAAATAACGACAGTTGCAGTACATGTATGACGACCCTGAGGCTGGGGGTACAGTACAATTCAATTGATTGACCCCATATCAAAACGCAgg                                                                                                                                                                                                                                                                         >  2:1032322/1‑290 (MQ=255)
                 ggcgGTAAATGCTTGCGAGCGCAACGGTAAACAGGCAATTGAGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAg                                                                                                                                                                                                                                                                      <  2:936005/290‑1 (MQ=255)
                      tAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCCGACCGGGAAATATCGCCAGTTGGGGTAACTGTATGACGCCCCTGCGGCTGGGTTTACATGAACGTTCACTGGTTGATCCCAATAGAAAAACGCTGGAAggtggt                                                                                                                                                                                                                                                                 >  2:452620/1‑286 (MQ=255)
                        gtggtagtgcaGCGCACGTGTAACAGGGCCGTGGGGATGGGGCGTAAACAGCTCGAACCAGAAGAAGGTAAAAAACGCCTGGAGCAACCGGGCGGCTTTATTCGTCAGGTTGTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTGACCCGTGTCGGCGGACCGGGAAATATCGCCAGGTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGaa                                                                                                                                                                                                                                                               <  2:363913/280‑1 (MQ=255)
                         ttgcttgcGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCACAATATCAAAACGCTGGAAGGCGTGAAg                                                                                                                                                                                                                                                              >  1:377155/1‑290 (MQ=255)
                               gcGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTc                                                                                                                                                                                                                                                        >  1:789540/1‑290 (MQ=255)
                                 gAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCAt                                                                                                                                                                                                                                                      >  1:567549/1‑290 (MQ=255)
                                 gAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCAt                                                                                                                                                                                                                                                      >  1:817339/1‑290 (MQ=255)
                                    cgaaaCTGGAAAACGGCCATGGGGATGGGGATGAAAAAACTCGATGCCGAAGAAGCTATAAAACGCCTGGATCAACACGTCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCAtttt                                                                                                                                                                                                                                                   <  2:265432/287‑1 (MQ=255)
                                         cTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTAACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCa                                                                                                                                                                                                                                              >  1:493559/1‑290 (MQ=255)
                                              aaaCGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGGCATTTTGACCAGCGAc                                                                                                                                                                                                                                         >  1:481076/1‑290 (MQ=255)
                                                aCGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCa                                                                                                                                                                                                                                       >  1:468071/1‑290 (MQ=255)
                                                  ggCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAgg                                                                                                                                                                                                                                     >  1:921508/1‑290 (MQ=255)
                                                      aTTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCa                                                                                                                                                                                                                                 >  1:579782/1‑290 (MQ=255)
                                                          tgATTGTGCTGAAAAGTCTGGATGCCGCGGAAGCTAAAAACCGCCTGGATCAACACGGCGTCTTTATTCGTCAGGGTTTAGACAAGGCGTAACCCATGGCCAAAGAGATCAGCAGTGAACTTCGGAACTCCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTc                                                                                                                                                                                                                             <  2:719334/290‑1 (MQ=255)
                                                          tcgggggactagaaaaGCCCGATGCCGCTGAGTCTAAGAAACGCCAGGAGCAGCCCGGCGTCGTTATTCGTCAGGTTTTAGACAAGGATAAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTc                                                                                                                                                                                                                             <  2:308151/278‑1 (MQ=255)
                                                              ggTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAATGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGttt                                                                                                                                                                                                                         >  1:768006/1‑290 (MQ=255)
                                                                   tGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTggg                                                                                                                                                                                                                    <  1:824237/290‑1 (MQ=255)
                                                                    gAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGAGTCATTTTGACCAGCGACCAGGTGCAGGTCGTGTTTggta                                                                                                                                                                                                                   >  1:303115/1‑288 (MQ=255)
                                                                              gATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAgc                                                                                                                                                                                                         <  1:110204/290‑1 (MQ=255)
                                                                                  ccGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCGGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCAt                                                                                                                                                                                                     <  1:389326/290‑1 (MQ=255)
                                                                                  ccGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCAt                                                                                                                                                                                                     <  1:856038/290‑1 (MQ=255)
                                                                                    gCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTGTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCg                                                                                                                                                                                                   <  1:343487/290‑1 (MQ=255)
                                                                                          gCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTGTTAGACAAGGAATAACCCATGGCCAAAGAGAGCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGc                                                                                                                                                                                             <  2:947170/290‑1 (MQ=255)
                                                                                                 aaCGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCg                                                                                                                                                                                      >  1:326002/1‑290 (MQ=255)
                                                                                                    gCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGGCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATg                                                                                                                                                                                   <  1:1001840/290‑1 (MQ=255)
                                                                                                     ccgtgtacAACACGGCGGCGTTATTCGTGAGGTGTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCAGTCTTACCCGTGTCGGTGGACCGGGAAATAGCGCCAGGTGTGGGAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATga                                                                                                                                                                                  <  2:735540/283‑1 (MQ=255)
                                                                                                      cTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATgag                                                                                                                                                                                 >  1:1029291/1‑290 (MQ=255)
                                                                                                      cTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAACCGCTGGAAGGCGTGAACGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATAGTGCCCCacacgagacgcg                                                                                                                                                                                 >  2:1067321/1‑279 (MQ=255)
                                                                                                        ggATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTACCTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAACATCCAAACGCTTGAAGCCTTGAAGGGCGTCATTCTGTCCCGCGACCCGCTGCCGGACGCTTCTCGCCCGGGTTAAACCCACCGTACCGCCAAAGCCATgcgcg                                                                                                                                                                               >  2:1019967/1‑290 (MQ=255)
                                                                                                             aaCACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAgctg                                                                                                                                                                          <  1:965744/290‑1 (MQ=255)
                                                                                                                  ggcggcTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGCGCGAGCTGCTggg                                                                                                                                                                     >  1:341273/1‑290 (MQ=255)
                                                                                                                    cggcTTTAGTCGTCAGGTTTTAGAAAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGgag                                                                                                                                                                   <  1:56817/290‑1 (MQ=255)
                                                                                                                     ggcTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGgaga                                                                                                                                                                  >  1:461301/1‑290 (MQ=255)
                                                                                                                               gTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTa                                                                                                                                                        >  1:207755/1‑290 (MQ=255)
                                                                                                                                tCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTAc                                                                                                                                                       >  1:996412/1‑290 (MQ=255)
                                                                                                                                tCAGGTTTTAGACAAGGAATAACCCATGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACa                                                                                                                                                      >  1:584420/1‑290 (MQ=255)
                                                                                                                                         aGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCg                                                                                                                                              <  1:305750/290‑1 (MQ=255)
                                                                                                                                            cAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGAAGCCGCAg                                                                                                                                           >  2:175279/1‑290 (MQ=255)
                                                                                                                                                     aCCCATGGCAAAAGAGTTCAGCAGTGAACTTCTGACCACCATTCTTACCCGGGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCGGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATcgccg                                                                                                                                  <  2:708316/290‑1 (MQ=255)
                                                                                                                                                       ccATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGAGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCgccgcc                                                                                                                                <  1:521790/290‑1 (MQ=255)
                                                                                                                                                        cATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACAAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTAAAGGATGCCGCAGCAATCGCCGccc                                                                                                                               >  1:984390/1‑290 (MQ=255)
                                                                                                                                                                   agagCAGCAGTGAACTTCTGAACACCATGCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGt                                                                                                                    <  1:91535/290‑1 (MQ=255)
                                                                                                                                                                           atctaaCTTCGGAGCACCTTTCTTGCCGTGTTCGGCTGGCCTGGAAATTTTGCCAGTTGTGGTAACTGTATGGCGTGGCTGCGTCTGGTTGTACATGACAGTTCACTGTTTGATCCAAATATCAAACCGCTGGAAGGCGGGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGGTTTTGTGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTaaa                                                                                                            <  2:921461/286‑1 (MQ=255)
                                                                                                                                                                              taaCTTCTGAACACCCTTCGTACCCGTTGCGGCGGACCGGGAAATATCGCCAGTGGTGGTAACTGTAGGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATAGCAAAACGCGGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGc                                                                                                         <  1:26238/289‑1 (MQ=255)
                                                                                                                                                                                aCTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCAAGCTGCTGGGAGAAGCCCCGGTACAGGATGCCGCAGAAATCGCCGCCACGAACAACCGTCAGTTAAAACCca                                                                                                       >  2:641455/1‑290 (MQ=255)
                                                                                                                                                                                          acCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGAGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGGACAGGATGCCGCAGAAATCGCCGCCCAGAACAGACGTCAGGTAAAAGACACACAAACCTc                                                                                             >  1:565993/1‑290 (MQ=255)
                                                                                                                                                                                            cATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAACGCGATGAGCGATCTGCTGGGAGCAGCCCCGGTACAGGCTGCCGCCGAAATCCCCGCCAAGAACAGACGTCCGTCAACAGCCCAACCACCCTCCg                                                                                           >  2:237354/1‑290 (MQ=255)
                                                                                                                                                                                             aTTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGACCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAACTCGCCGCCCAGAACAACCGTCAGTTAAACGCCAAACAACCCTCCgg                                                                                          >  2:1030137/1‑290 (MQ=255)
                                                                                                                                                                                                       gtgtCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGAAAAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATAGTGCCGAAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTAAAGGATGACGCAGAAATAGCAGCACAGAACAAACGTAAGTTAAAAGCAAAACACACATACGGCATGcaaaaa                                                                                >  1:651295/1‑290 (MQ=255)
                                                                                                                                                                                                        tgtCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAAt                                                                               <  1:213809/290‑1 (MQ=255)
                                                                                                                                                                                                              gcggACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTtctc                                                                          <  1:292215/290‑1 (MQ=255)
                                                                                                                                                                                                                ggACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGGTTTGGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCGGGGACAAGCAACGGTAAAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCGGTTAAAAGGCAAACAACACAACGGCGGCcaacaaatcacccc                                                                        >  2:30041/1‑282 (MQ=255)
                                                                                                                                                                                                                 gACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGcc                                                                       <  1:355171/290‑1 (MQ=255)
                                                                                                                                                                                                                     gggAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCaact                                                                   >  1:269009/1‑288 (MQ=255)
                                                                                                                                                                                                                            atCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGccac                                                            <  1:67710/290‑1 (MQ=255)
                                                                                                                                                                                                                               gctatttGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCCATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCAt                                                         <  1:54421/285‑1 (MQ=255)
                                                                                                                                                                                                                                ccAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAACGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCCGAAATCGCCGCCCCGAACCAACGTCAGTTACAAGCCAAAACAACCTCCGGCGGTCACAAACTTCTCGCCAACGTCGCCCCCATc                                                        >  2:123738/1‑290 (MQ=255)
                                                                                                                                                                                                                                 cAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCt                                                       >  1:865362/1‑290 (MQ=255)
                                                                                                                                                                                                                                          tAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCCAGCGTCTGGGAGACGCCCCGGGACCGGCTGCCGCCGAAATCGACGCACAGCACAACCGTCAGTTACAAGACACACCAACCCCCGCCGTCCAACAATTTCCCGCACACTTCGacacacactcacagcccc                                              >  2:709925/1‑276 (MQ=255)
                                                                                                                                                                                                                                                      gcgcCTGCGGCGGGGTTGAAAGTACAGTTCACTGGTGGATCCCAATAGCAAAACGCTGGAAGGCGGGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGtt                                  <  2:353342/290‑1 (MQ=255)
                                                                                                                                                                                                                                                       cgcCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGACCAAACGTCAGTTAAAAGCCAAACAAACCTACGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCCCGCCCCTGATCCCCggctc                                 >  2:1035166/1‑287 (MQ=255)
                                                                                                                                                                                                                                                          cTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTAt                              <  1:972036/290‑1 (MQ=255)
                                                                                                                                                                                                                                                              gtgttggTGTACAGGGCAGTGTACTGGTGGATCCCATGAGCAAGACGCTTGACGTCTGGAAGTGCGTCATATTGACCAGCGACCAGGGTCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTgcc                          <  2:671869/285‑1 (MQ=255)
                                                                                                                                                                                                                                                                 tGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTgccgcc                       <  1:452620/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                      gtACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTct                  >  1:768434/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                      gtACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTct                  <  1:107183/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aTGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTctgctg              >  1:272624/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                            gACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTctgctgct            >  1:567034/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                            gACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCACCTTCCCGCCGCGCATCCCCCGTTTCATTGCCCCCCTTctgctccc            >  2:428016/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                               aGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCCGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTggg         >  1:101967/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                               aGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGACGCACCGGTCCCGGATGCCGCAGAAATCGCCGCCCAGACCAAACGTCAGTTACCAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCACCATTCGCCACCATCTTCCCGCCGCTGCTCACCGGTTTTATTCCCGCCAGTCTGCTGCCggc         >  2:681870/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                 ttCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGa       >  2:580985/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                  tCACAGGTTGAACCCAAAGTGAAAAGGCTGGAAGGCGTGAAGGGCGTCATTGTGACCAGCGACCAGGTGCAGGTCGTGTTTGGGCCGGGTAAAGCGCAGCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGAt      <  2:182582/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      tGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGACGCTGATCCCCGGTATTATTGCCGACGGTCTGCTGCGGGGGATAGCg  >  1:632292/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                          
GCAGAGCAAGCGGAAGCGGCGTTAATTGCTTGCGAGCGCAACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCATCTTCACGCCGCTGATCCCCGGTTTTATTGCCGCCGGTCTGCTGCTGGGGATAGCG  >  NZ_LN832404/2549396‑2549962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: