Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 3,419,910 C→T 9.2% pseudogene (74/81 nt) EK12AG100_RS25785 → paraslipin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324043,419,9100CT 9.2% 222.0 / 10.3 76pseudogene (74/81 nt)EK12AG100_RS25785paraslipin
Reads supporting (aligned to +/- strand):  ref base C (28/41);  new base T (4/3);  total (32/44)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACT  >  NZ_LN832404/3419623‑3420199
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                  
gatgagtgaGCTGCGCCTGGTGAATTTTTGGCGTATCCGAAATCAGAGCGTACGAGGGCAATTTTATCTCAGATAATCCATTAATTGGATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTGCGGATGGCCTTTCGCTTGATTTGATGTCTGACAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGGTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCg                                                                                                                                                                                                                                                                                                  <  2:802687/282‑1 (MQ=21)
  ggAGCAAGGTGGATCTGATGACTTTTGTGCTCATACTAGATCATAGCGGACTGTGTAATTTGTGTCGCAGGTATTCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCATGCCTGTCGGATGGCCTTTCGCTTGATGTGATGTCTGGCAGTGTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGAc                                                                                                                                                                                                                                                                                                <  2:214246/290‑1 (MQ=18)
       cagttcaatcaactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAA‑CGAAAGGCCCAGTCTTTCGACTGAGc                                                                                                                                                                                                                                                                                           <  1:934696‑M2/149‑1 (MQ=255)
           tGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCttt                                                                                                                                                                                                                                                                                       <  1:225951/290‑1 (MQ=255)
            gCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGACtttt                                                                                                                                                                                                                                                                                      >  1:822344/1‑290 (MQ=255)
            gCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAACCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTACCGGCGGATTTGTCATACTCGGGAGAGTGTTCACCGACAAACAACAGCTAAAA‑CAAAAGGCCAAGTCTTCCGAACGACCCtttt                                                                                                                                                                                                                                                                                      >  1:668938/1‑290 (MQ=255)
                caactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAA‑CGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGttt                                                                                                                                                                                                                                                                                  >  1:947455‑M2/133‑290 (MQ=255)
                 tgatgaATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCCGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGtttt                                                                                                                                                                                                                                                                                 <  1:176918/290‑1 (MQ=255)
                 aactgtttaccttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGCTTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAA‑CGAAAGGCACAGTCTTTCGACTGAGCCTTTCGtttt                                                                                                                                                                                                                                                                                 >  1:633647‑M2/132‑290 (MQ=255)
                  actgtttccctggtgtggcgccttccagaggatcttcaccgcttgccagcggcttaccgtccagtgccaccatccagtgcagggtctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCATGTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAA‑CGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTa                                                                                                                                                                                                                                                                                <  2:142571‑M2/160‑1 (MQ=255)
                    gtatttgtttGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTAtt                                                                                                                                                                                                                                                                              <  1:30587/283‑1 (MQ=255)
                     gaATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTAttt                                                                                                                                                                                                                                                                             <  1:227982/290‑1 (MQ=255)
                       aTTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCAAGTATTCCGACTGAGCCTTTTGTTTTATTTGa                                                                                                                                                                                                                                                                           >  1:336317/1‑290 (MQ=255)
                         accttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAA‑CGAACAGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATg                                                                                                                                                                                                                                                                         >  1:939432‑M2/124‑290 (MQ=255)
                          ttttGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAACCAACCGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGt                                                                                                                                                                                                                                                                        >  1:680189/1‑290 (MQ=255)
                           tttGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTc                                                                                                                                                                                                                                                                       <  2:625536/290‑1 (MQ=255)
                           tttGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTCTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTc                                                                                                                                                                                                                                                                       <  2:299184/290‑1 (MQ=255)
                            ttgtggagcgacatccagaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAACAAACAGCTAAAA‑CGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGCTGCCt                                                                                                                                                                                                                                                                      >  1:729831‑M2/121‑290 (MQ=255)
                                 catcatAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAg                                                                                                                                                                                                                                                                 <  1:682103/290‑1 (MQ=255)
                                 cATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGTCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCCCAACGTTCAACTCCGCTCCCGGCCGATTTGTCCTACTTCGGAGAGTGTTCACCGACCAACCACACATAAAACCAAAAGCCCCATCCTCCCAACTG‑GCCCTTTGttttttttttttttttggcg                                                                                                                                                                                                                                                                 >  2:525696/1‑278 (MQ=21)
                                 cATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTTCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCCAATCCCCTCCCGGCGGGTTTTTCCTCCCCGGGAGAGTGGTTCCCCACAAACAACCGATACAA‑CAAAAAGCCCAGTCTCCCACCACAGCCCtttttttttttttttatcttgcca                                                                                                                                                                                                                                                                 >  2:6248/1‑278 (MQ=21)
                                    ccTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCCAATCCGCCCCCGGCGGATTTTGCCTACTCGGGTGAATTTTCCCCGCCCACCCACAGGTAAAA‑CAAAAAGCCCAGGCTTCCGCCTGACCCTTTTGTTTTTTTTGATGTCTTGCTGTTc                                                                                                                                                                                                                                                              >  2:964396/1‑290 (MQ=21)
                                         gtaagggggtcctgggggATTGTTGTCGAAGGTAATCCATTAATTGAAGGGTAGTTCGAACAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTAc                                                                                                                                                                                                                                                         <  2:323252/276‑1 (MQ=17)
                                         aTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTAc                                                                                                                                                                                                                                                         >  1:391007/1‑290 (MQ=255)
                                           cAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATTTCTGGCAGTTCCCTActc                                                                                                                                                                                                                                                       >  1:50429/1‑290 (MQ=255)
                                            agaggcacttcaccgcttgccagcggcttaccatccagcgccaccatccagtgcaggagctccgatgataagctgtcaaacatgagaattccgatccccaattcCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAA‑CGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTtttatttgatgcctggggaattaattcctg                                                                                                                                                                                                                                                      <  1:460076‑M2/186‑15 (MQ=255)
                                                 gTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGCTGTCTGGCAGTTCCCTAATCTCG‑CAt                                                                                                                                                                                                                                                >  1:982148/1‑290 (MQ=255)
                                                      aGGGCATTTTTATCGAAGGTAATCAATTAATTGAATGTTAGTGCGAAAAGCAAAAAGGCAGTCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCTGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGga                                                                                                                                                                                                                                           <  2:1001226/290‑1 (MQ=255)
                                                        ggCATTTTTATCGCAGGTAATCCATTAATGGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAAAAAAAGATAAAA‑AAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTCATCTCG‑CCTGGGgaaa                                                                                                                                                                                                                                         >  1:793754/1‑288 (MQ=255)
                                                            tttttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGAcccc                                                                                                                                                                                                                                     >  1:367172/1‑290 (MQ=255)
                                                            ttgttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGAcccc                                                                                                                                                                                                                                     <  1:498146/287‑1 (MQ=255)
                                                                 aTCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACt                                                                                                                                                                                                                                <  1:514822/290‑1 (MQ=255)
                                                                 aTCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACt                                                                                                                                                                                                                                >  1:687950/1‑290 (MQ=255)
                                                                       ggTATTCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATc                                                                                                                                                                                                                          <  1:758805/290‑1 (MQ=255)
                                                                       ggTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATc                                                                                                                                                                                                                          <  1:888054/290‑1 (MQ=255)
                                                                            tCCATTACTTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGgcgc                                                                                                                                                                                                                     >  1:595728/1‑290 (MQ=255)
                                                                              cATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTa                                                                                                                                                                                                                   >  1:229287/1‑290 (MQ=255)
                                                                               aTTAATTGTATGTTAGTTCGAAACGCAAAAAGGGCATCCTTTCGGATGGCCTTTCGCTTGATTTGAGGGCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTAc                                                                                                                                                                                                                  <  2:772414/290‑1 (MQ=255)
                                                                               aTTAATTGAATGTTAGTTAGAAAAGCAAAAAGGTCATCCTTTCGGATGGCCTGTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTAc                                                                                                                                                                                                                  <  1:733216/290‑1 (MQ=255)
                                                                                     tGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCCAATCCGCCCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCCCCGACCAAAAACAGATAAAA‑CAAAAAGCCCAGTCCTCCGACTGCGCCTTTTGTTTTATTTGAAGTCTGGCAGTTTCCTACTCTCG‑CATGGGGAGACCCCCACCCACCATCGgcctcaccacctc                                                                                                                                                                                                            >  2:418211/1‑279 (MQ=17)
                                                                                      gAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                                                                                                                                           >  1:819624/1‑290 (MQ=255)
                                                                                       aaTGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTTCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCCGATTTTTCCTACTCGGGAGAGTGTTCACCCACCAACAACAGATAAAA‑CAAAAGGCCCAAGCTTTCCACTGAGCCTTTTGTTTTATTTGATGTCCTGCAGTTCCCTCCTCTCC‑CATGGGGAGACCCCCCCCCACCATCggaccaccggctcttt                                                                                                                                                                                                          >  2:518368/1‑276 (MQ=17)
                                                                                             aGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACCCTACCATCGGCGCTACGGCGTTTCACTTCt                                                                                                                                                                                                    >  1:373031/1‑290 (MQ=255)
                                                                                              gTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACCACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTAACCCCG‑CCTGGGGAGACCCCCCACTACCATCGGCGCTACGGCGGTGCACTTCTg                                                                                                                                                                                                   >  1:60033/1‑290 (MQ=255)
                                                                                                   aaaaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTc                                                                                                                                                                                              <  1:501020/290‑1 (MQ=255)
                                                                                                            aaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGCGTTCGGCCTgggg                                                                                                                                                                                     >  1:714081/1‑290 (MQ=255)
                                                                                                               gCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCa                                                                                                                                                                                  <  1:95647/290‑1 (MQ=255)
                                                                                                                 cATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAATTCGGCATGGGGTCAgg                                                                                                                                                                                >  1:953156/1‑290 (MQ=255)
                                                                                                                  aTCCTTTCGGATGGCCTTTCGCTGGAGTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt                                                                                                                                                                               <  1:985156/290‑1 (MQ=255)
                                                                                                                   tCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGAGCTACGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTg                                                                                                                                                                              >  1:718726/1‑290 (MQ=255)
                                                                                                                           gtgggCCGTGCGCTGGATTGGTGGTCTGGCAGTTTATGGCGGGCGTCCTCACCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccacc                                                                                                                                                                      <  2:391007/287‑1 (MQ=255)
                                                                                                                           gATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccacc                                                                                                                                                                      <  1:159325/290‑1 (MQ=255)
                                                                                                                                  tttCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCCCCCGGCGGATTTTTCCTACTCGGGAGGGGGTTCACCGACCAACAACAGATAAAA‑CAAAAGGCCCAGTCGTCCGACTGAGCCTTTTGTTTTCTTTGATGACTGGCCGTTCCCTACCCCCGCCAGGGAGGGACACAAC‑CCACCCACGGCCGTACCGCGTTTCAACTCTGAGATCGGCATCGGGGCAgggggggacagccggctac                                                                                                                                                               >  2:2687/1‑277 (MQ=11)
                                                                                                                                      gggggatttgtgGGCTGGCAGTTTAGGGCGGGCGGCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTgcc                                                                                                                                                           <  2:50429/286‑1 (MQ=255)
                                                                                                                                      gCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTgcc                                                                                                                                                           >  1:617484/1‑290 (MQ=39)
                                                                                                                                                  gTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAAtt                                                                                                                                               <  1:883458/290‑1 (MQ=39)
                                                                                                                                                       gCAGTTTATGGCGGGCGTCCTGCCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCtttt                                                                                                                                          <  1:77388/290‑1 (MQ=39)
                                                                                                                                                          gTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTa                                                                                                                                       >  1:819967/1‑290 (MQ=40)
                                                                                                                                                           tttCTGGCGTGCGTCCTGCCCGCCTCCCGCCGGTCCGTTGCTTCGCAACGTTCAAATCCGCTCCCTGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTaa                                                                                                                                      <  2:819624/290‑1 (MQ=255)
                                                                                                                                                            ttATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCCGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCCGACAAATTCTTTTCTAAt                                                                                                                                     >  1:851156/1‑290 (MQ=255)
                                                                                                                                                              gtGGCGGCCGTCCGGCCGTCCACCCTCCGCGCTGTTGCTTCTCAACGTTCAAATCCGCTCACGGCGGATTTGTCCTCCTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCATTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGGTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCt                                                                                                                                   <  2:582746/289‑1 (MQ=14)
                                                                                                                                                                    ggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGaa                                                                                                                             <  1:267958/290‑1 (MQ=255)
                                                                                                                                                                      cgTCCTTCCCTCCACCCTCCGTGCCGTGGCTTCGCAACTTTCAAATCCGCTCCCGGCGTATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACt                                                                                                                           <  1:952963/290‑1 (MQ=255)
                                                                                                                                                                        tCCTGCCCGCCACCTTCCGGGGCGTTGCTTCGAGACGTTCCAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt                                                                                                                         <  1:7092/290‑1 (MQ=255)
                                                                                                                                                                               cGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTac                                                                                                                  >  1:122463/1‑289 (MQ=255)
                                                                                                                                                                                            gCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTTCTGa                                                                                                     >  1:126674/1‑290 (MQ=255)
                                                                                                                                                                                             ccGTGGCTTCGCAACGTTCACATCCGCTCCCCGCGGATTTGTCGTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAATGCCCAGTCTTCCGACTGAGCCTTTTGGTGTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGAt                                                                                                    <  2:872169/290‑1 (MQ=255)
                                                                                                                                                                                               gcgtcTTCGCAACGTTCAAATCCGGTACCGGCGGATTAGTCCTACTCGGGAGAGTGTGCACCGACAAACAACAGATAAAA‑CAAAAGTCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGAGGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATAc                                                                                                  <  2:258027/286‑1 (MQ=255)
                                                                                                                                                                                                ttGCTTCCCAACGTTAAAATCCGCTCCCGGTGGTGTTGTCCTACTCGGGAGATTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGTCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATAcc                                                                                                 <  1:88300/290‑1 (MQ=255)
                                                                                                                                                                                                ttGCGTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTAATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATAcc                                                                                                 <  1:199938/290‑1 (MQ=255)
                                                                                                                                                                                                         aaCGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGa                                                                                        >  1:857313/1‑290 (MQ=255)
                                                                                                                                                                                                                    ccGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCt                                                                             <  1:525696/290‑1 (MQ=255)
                                                                                                                                                                                                                           cggcggATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCtt                                                                      <  1:96591/290‑1 (MQ=255)
                                                                                                                                                                                                                            cgcggAATGGTCTTACCGGGGAGAGGGTTCCGCGACAAAAAACAGCGAAAA‑CAAAAGGCCCAGGCTTCCGACTGAGCCTTTTGTTGTATTTGATGTCTGGCAGGTCCCTACGCTCG‑CATGGGGGGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCGGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTa                                                                     <  2:437803/289‑1 (MQ=18)
                                                                                                                                                                                                                                       ccTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAACTTCTTTTCTAATCTGCCGAACTTTCACCTAAAACGTGGTGCTGATACACAGAGTCGAACTGGGGACCTCACACTTACCAAGGGTgcg                                                          >  1:698415/1‑290 (MQ=255)
                                                                                                                                                                                                                                                gagaGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCaa                                                 <  1:978263/290‑1 (MQ=255)
                                                                                                                                                                                                                                                 agagTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAAc                                                <  1:1024437/290‑1 (MQ=255)
                                                                                                                                                                                                                                                  gagTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACt                                               >  1:873934/1‑290 (MQ=255)
                                                                                                                                                                                                                                                         cACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTTTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCat                                        <  1:569435/290‑1 (MQ=255)
                                                                                                                                                                                                                                                             gACAAACAACAGATAAAA‑CAAAAGGGCCAGTCTTCCGACTGAGCCTTTTGTTGTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCa                                    <  2:828091/290‑1 (MQ=255)
                                                                                                                                                                                                                                                              acaaacaaCAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCCCCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAATAGTGATGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGATGCGCTCTACCAAATGAGCCATATCAg                                   >  1:631844/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                  acaacaGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGATGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCCAGGGTGCGCTCTACCAACTGAGCCACATACGCACg                               >  1:750091/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                       aGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTaaa                          <  1:768004/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                              a‑CAAAAGGCCCCGTGCTCCGACTGAGCCTTTTGTTTTAGTTGATGTCTGGCAGTTCCCTCCTCGCG‑CATGGGGAGACCCCACACGACCATCGGCGCTACGGCGTTTCACTGCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATg                   <  2:857215/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                                cAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGTGTATCTCACCCTTTCCAAAGGTCCTCTCTACCACCTGAGCCATATCATCTCGCTCACTTTGATGc                  >  1:1015311/1‑290 (MQ=21)
                                                                                                                                                                                                                                                                                                ccGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCCGCACGCTAACTTTGATGCCTGGCAGTTCCCTAct  >  1:480848/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                  
GTGGAGCAAGCTGCACCTGATGAATTTTTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAA‑CAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCG‑CATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACT  >  NZ_LN832404/3419623‑3420199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑