Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 3,917,872 T→C 75.6% Q231R (CAG→CGG) ‡ atpB ← F0F1 ATP synthase subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324043,917,8720TC75.6% 146.9 / 52.8 82Q231R (CAG→CGG) ‡atpBF0F1 ATP synthase subunit A
Reads supporting (aligned to +/- strand):  ref base T (8/12);  new base C (19/43);  total (27/55)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

GCCTTCCAGGAATTTACCCCCGAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGG  >  NZ_LN832404/3917588‑3918155
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                            
gcCTTCCAGGAATTTACCCCCGAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGc                                                                                                                                                                                                                                                                                         <  1:31789/290‑1 (MQ=255)
gcCTTCCAGGAATTTACCCCCGAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGc                                                                                                                                                                                                                                                                                         <  2:77122/290‑1 (MQ=255)
 ccTTCCAGGAATTTACCCCCGAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCa                                                                                                                                                                                                                                                                                        <  2:846654/290‑1 (MQ=255)
     ccAGGAAGTTACCCCCGAGGAGGCCGAACCCGATCGCAGAACCGATTGCCGCCAGACCCATAATCACAGGGGCAGCAATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAg                                                                                                                                                                                                                                                                                    <  2:288859/290‑1 (MQ=255)
            tttACCCCCGAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCa                                                                                                                                                                                                                                                                             <  1:570116/290‑1 (MQ=255)
             ttACCCCCGAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGAACCACTGTGACCACCACGGCCACAGACCAGcca                                                                                                                                                                                                                                                                            >  1:573501/1‑288 (MQ=255)
                  cccGAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGa                                                                                                                                                                                                                                                                       <  1:575972/290‑1 (MQ=255)
                      tggATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGa                                                                                                                                                                                                                                                                   <  1:899290/289‑1 (MQ=255)
                            ccGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCa                                                                                                                                                                                                                                                             <  1:129948/290‑1 (MQ=255)
                            ccGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCa                                                                                                                                                                                                                                                             >  1:680022/1‑290 (MQ=255)
                                tACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTc                                                                                                                                                                                                                                                         <  2:771066/290‑1 (MQ=255)
                                   cgtggccagcaCCGATGACAGCCAGACCAGTCGAGCCAGCGGCTGCCATGTACAGCAGTTCCATATTCATGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAACCGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCAcc                                                                                                                                                                                                                                                      <  2:483718/284‑1 (MQ=255)
                                    gATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCCGGATCCCCTGTAACCACCACGGCACCAGACCAGCAATCAGAATGAAAATCCGccacccg                                                                                                                                                                                                                                                     >  2:410786/1‑284 (MQ=255)
                                      tCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATCTTCAGGTTTTCCATGACAGCCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAATTTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGACTAGCAAGGCTTGCAGTGTAATGAACAGGACGCGGAAATTGGCACACGGCACATCCAGGTCCCATTGTTACCACCTCGGAAACAAAACAACAAACATAATGcaaagttgttacacctc                                                                                                                                                                                                                                                   >  2:527631/1‑277 (MQ=255)
                                        gcagcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGGCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCAt                                                                                                                                                                                                                                                 <  1:548043/290‑1 (MQ=255)
                                         cagcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATa                                                                                                                                                                                                                                                <  1:78450/290‑1 (MQ=255)
                                          agcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATAc                                                                                                                                                                                                                                               <  1:344584/290‑1 (MQ=255)
                                           gcaCCGAGTGCCGCCAGACCCGCAATCACGGCGGCAGCCATGTACAGCAAATCCATATTCAGGTTTGCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACa                                                                                                                                                                                                                                              <  2:770741/290‑1 (MQ=255)
                                                gATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTAACCGGCATACATGTTa                                                                                                                                                                                                                                         >  1:29428/1‑290 (MQ=255)
                                                   tGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCg                                                                                                                                                                                                                                      <  1:938829/290‑1 (MQ=255)
                                                           gACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGCATGAAAATCAGATCACCGGCATACATGTTACCGAACAGTCg                                                                                                                                                                                                                              >  1:649021/1‑290 (MQ=255)
                                                                      acaGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTg                                                                                                                                                                                                                   <  2:636998/290‑1 (MQ=255)
                                                                           ggCAGCCATGTACAGCAGATCCATAGTCAGGTTTTCCATGCTAGGCTCCAGGTTGTTTCATTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACt                                                                                                                                                                                                              <  2:316174/290‑1 (MQ=255)
                                                                                  aTGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGTAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTgg                                                                                                                                                                                                       >  1:330807/1‑290 (MQ=255)
                                                                                      aCAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCATTTTGTTTCAGTTAAATCGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGAcag                                                                                                                                                                                                   <  1:869223/290‑1 (MQ=255)
                                                                                         tcttaTCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACTTAGTAGTGTTGTTAAATTAATGTTCTACAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGAcagcag                                                                                                                                                                                                <  2:767401/286‑1 (MQ=255)
                                                                                                 atatTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTAccc                                                                                                                                                                                        <  1:25135/290‑1 (MQ=255)
                                                                                                   agtgatgTTTTCCAGTACAGTTTCCAGTTTGTTTTAGTGAAAACGTAGGTGTGTTGGTAAATTAAAGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCTTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCt                                                                                                                                                                                      <  2:1055538/284‑1 (MQ=255)
                                                                                                      cAGGTGTTCCATGACGTGTTCCAGTGTTTTGCGGGTAACACGTAGGAGTGTTGGTAAAGTAATGGTCTTCAGACGACATCGACAGATAGACTATCGTCAGAACCAGGAAGAGGAAGGCGGGCAGCGTAATGAGCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCa                                                                                                                                                                                   <  2:1045358/290‑1 (MQ=255)
                                                                                                             tCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTa                                                                                                                                                                            >  1:494429/1‑290 (MQ=255)
                                                                                                              ccATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTaa                                                                                                                                                                           >  1:525130/1‑290 (MQ=255)
                                                                                                               ctggACATTCTCCAGTTTGTGTCAGTGAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAg                                                                                                                                                                          <  2:1042937/287‑1 (MQ=255)
                                                                                                               cATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAg                                                                                                                                                                          <  1:363138/290‑1 (MQ=255)
                                                                                                                 tGACATTCTCCATTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGtt                                                                                                                                                                        <  1:765978/290‑1 (MQ=255)
                                                                                                                    cagtcgcatgtttttttAAGTTAAAACGTAGTAGTGGTGGTAAATTAAGTGTCATCAGACGCCATCGACAGATAGACGATCGTCAGAAACAGGAAGATGAAGGCTTGCAGCGTAATGAGCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGAc                                                                                                                                                                     <  2:309191/281‑1 (MQ=255)
                                                                                                                          ccAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCCAACAGAATGAAAATCCGCTCACCGGCATACATGTCACCGAACAGTCGCACACCGAGAGAACCGGGTTTGGACAGCAGGCTTACCACATCACGAATTAAGTTGACAGGaac                                                                                                                                                               >  2:271442/1‑289 (MQ=255)
                                                                                                                             gtttgtttCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGaa                                                                                                                                                           <  1:526962/290‑1 (MQ=255)
                                                                                                                                     cAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGt                                                                                                                                                   <  1:881221/290‑1 (MQ=255)
                                                                                                                                      aGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTg                                                                                                                                                  >  1:93064/1‑290 (MQ=255)
                                                                                                                                          aaaaCGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCCGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAACCCGCGTGAAACTGGTTTGGACAGCCGCCTTACCCCTTCAAGGATTAAGATGACAAGAATGTAAGGCACAGTAAtt                                                                                                                                               >  2:551010/1‑290 (MQ=255)
                                                                                                                                          aaaaCGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCCGCTCACCGGAATACATGTTACCGAAAAGTCGCAAACCGAGTACAACTGGTTAGAACAGAAGGCATACCCATTCCAGGCATAAGTTGCCAGGACTGACCGccagagcattg                                                                                                                                              >  2:440882/1‑281 (MQ=255)
                                                                                                                                           aaaCGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGa                                                                                                                                             <  1:297281/290‑1 (MQ=255)
                                                                                                                                               gtagtaTTGTGGTGAAATTAATTTTCTTCAAACGCCATCGACAGATAGACGATCGTCAGACCCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACgg                                                                                                                                         <  1:839372/290‑1 (MQ=255)
                                                                                                                                                                ttAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCACTCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTATGGACAGCAGGCTTACCCCTTCACGGATCAAGTTGCCAGGAATGAACGCCCAGTGATTGCACGGCGGCAGCGGAAACCCtt                                                                                                                        >  2:426540/1‑290 (MQ=255)
                                                                                                                                                                    agTTCGTCAGACGCAATCAACAGATAGCCGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGGAATTATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGGTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGt                                                                                                                    <  2:411961/289‑1 (MQ=255)
                                                                                                                                                                     gTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTg                                                                                                                   <  2:494429/290‑1 (MQ=255)
                                                                                                                                                                       tcgtcAGACACACTCCACAGATAGACGATCGTCAGAACCATGAAGATAAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATACACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGaa                                                                                                                 <  2:374027/287‑1 (MQ=255)
                                                                                                                                                                        gtcccgACGCCATCGACATATGGACGAACGTCAGAACCATGAAGAAGAAGGCTTGCAGCGTAAGGACGAGGATGTGGAAAATGGCCCACGGCACATGCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAg                                                                                                                <  2:129084/285‑1 (MQ=255)
                                                                                                                                                                           cAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAgccg                                                                                                             <  1:20327/290‑1 (MQ=255)
                                                                                                                                                                                gCCATCGACAGATAGACGATCTTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGAt                                                                                                        <  1:839430/290‑1 (MQ=255)
                                                                                                                                                                                gCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGACACGTCGCAACCCGAGTGAAACTGGTTTGGACAGCACGCTTCCCGCTTCACGGATTAAGTTGACAGGAATGAACGCCCAGTGATAGACCGGCTGCACCGCCAACCATATCGTGAAgaccctgtg                                                                                                        >  2:1048538/1‑282 (MQ=255)
                                                                                                                                                                                gCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGAt                                                                                                        >  1:502331/1‑290 (MQ=255)
                                                                                                                                                                                gCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGAt                                                                                                        >  1:199315/1‑290 (MQ=255)
                                                                                                                                                                                   aTCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTCTCGTGAAGCCGCCGATGcc                                                                                                     >  1:1028339/1‑290 (MQ=255)
                                                                                                                                                                                     tgACAGTTAGACGATCGTCAGAACCAGGAAGAGTGACGCGTGGAGCGTAAGAATCAGGATGTCGATAATGGCCCACGGCACAGTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCGCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCtt                                                                                                   <  2:40931/289‑1 (MQ=255)
                                                                                                                                                                                         tgATAGACGATCGTCAGAACCATGAAGATACAGGCTTTCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCAt                                                                                               <  2:408865/289‑1 (MQ=255)
                                                                                                                                                                                         aGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGCGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGGTATTGAACGGCTGCAGCGTCAACTCATTCGTGAAACCGCCGCTGCCTTGCAt                                                                                               >  2:601440/1‑290 (MQ=255)
                                                                                                                                                                                               aCGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGAt                                                                                         <  1:869053/290‑1 (MQ=255)
                                                                                                                                                                                                  aTCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCt                                                                                      <  2:680022/290‑1 (MQ=255)
                                                                                                                                                                                                   tCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGt                                                                                    <  1:1001648/290‑1 (MQ=255)
                                                                                                                                                                                                    cGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGt                                                                                    <  2:181413/290‑1 (MQ=255)
                                                                                                                                                                                                       cAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCCGTGATTGACCTGCTGACGCGTCAACTCCTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTTTact                                                                                 >  2:313108/1‑288 (MQ=255)
                                                                                                                                                                                                       cAGAACAATGAAAATGAAGGCTTGAAGCGGAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGa                                                                                 <  2:35495/290‑1 (MQ=255)
                                                                                                                                                                                                         gAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCCGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAAc                                                                               >  1:189966/1‑290 (MQ=255)
                                                                                                                                                                                                          gaCCATGAAGAGGAAGGCGTACAGGGTAAAGATCAGGATGTGGAAAATGGCCCACGGGACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACa                                                                              <  2:1028429/289‑1 (MQ=255)
                                                                                                                                                                                                               tgaagatgaagGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATc                                                                         <  2:745859/290‑1 (MQ=255)
                                                                                                                                                                                                               tgaagatgaagGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATc                                                                         <  1:875387/290‑1 (MQ=255)
                                                                                                                                                                                                               tgaagatgaagGCTTGCAGCGTAAGGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATc                                                                         <  1:259534/290‑1 (MQ=255)
                                                                                                                                                                                                                  agatgaagGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAgg                                                                      >  1:832552/1‑290 (MQ=255)
                                                                                                                                                                                                                       agaggttGAAGAGTAATGATCAGGGGGTGGAAAAGGGCCACAGGAACATGCAGGAGCAACTGCTACACCTGCGGCAAAAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACAGGGTAACGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATaaa                                                                 <  2:1064785/285‑1 (MQ=255)
                                                                                                                                                                                                                        agGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAAt                                                                <  1:529025/290‑1 (MQ=255)
                                                                                                                                                                                                                           cTTGCAGATTAATGATACGGAGTTGAAAAATGGCCAACTGACCATTCAGGGTCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACg                                                             <  2:15646/290‑1 (MQ=255)
                                                                                                                                                                                                                                   gTAATGGTCCGGATGTGGAAAAGGGCCCCCGCCACATTCACGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGc                                                     <  2:423683/290‑1 (MQ=255)
                                                                                                                                                                                                                                   gTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCGCACCGGAATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGc                                                     <  1:137519/290‑1 (MQ=255)
                                                                                                                                                                                                                                       tGATCAGGATGTGGAAAATTGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATa                                                 <  1:511900/290‑1 (MQ=255)
                                                                                                                                                                                                                                         aTCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACCGGAATGCACGACCAGTGATTGAACGGCTGCAGCGGCAACGCGTCCGTGACGACGCCGATGCCATTCATGTTGATTCTGTAGAACAGACTCAGGAACAATACGCACACTGCAATCGa                                               >  2:453213/1‑290 (MQ=255)
                                                                                                                                                                                                                                            aGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAg                                            <  1:76155/290‑1 (MQ=255)
                                                                                                                                                                                                                                                tgtgGAAAATGGCCCACGGCACATTCAGGATCCACTGTAACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGtt                                        <  1:677124/290‑1 (MQ=255)
                                                                                                                                                                                                                                                    gAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGt                                    >  2:218333/1‑290 (MQ=255)
                                                                                                                                                                                                                                                          ggCCCACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGCCAGCAGGCTTAACCCTTCACAGATTAAGTTGACAGGAATGAACGCCAAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGACGCCGCCGATGCATTTCATTTTGATGCTGTAGAACAGCATAAGGATACATACGCCCAGCGCCATCGACACCGTTAACTTAACGt                              >  2:618090/1‑290 (MQ=255)
                                                                                                                                                                                                                                                            cgcACGGCACAGTCAGGAGCCACTTGAACAACCAAGGCAACAGACCTGCAAGCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGGGTGAAACGTGTTTGGACAGCAGGCTGACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTcc                            <  2:725123/288‑1 (MQ=255)
                                                                                                                                                                                                                                                            cccACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAACACCGCCCAGTGCCATCATCAGCGTTACggtacagtca                            >  2:548065/1‑281 (MQ=255)
                                                                                                                                                                                                                                                              cACGGCACATTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGc                          >  1:827134/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                     caTTCAGGATCCACTGTGACCACCACGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGa                   >  2:665322/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                      gACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTgcagg  <  1:795518/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                            
GCCTTCCAGGAATTTACCCCCGAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAATTAATGTTCTTCAGACGCCATCGACAGATAGACGATCGTCAGAACCATGAAGATGAAGGCTTGCAGCGTAATGATCAGGATGTGGAAAATGGCCCACGGCACATTCAGGATCCACTGTGACCACTGCGGCAACAGACCAGCAATCAGAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGG  >  NZ_LN832404/3917588‑3918155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: