Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	4,303,694	C→T	81.2%	L193L (CTG→CTA)	alsK ←	allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	4,303,694	0	C	T	81.2%	183.6 / 35.5	80	L193L (CTG→CTA)	alsK	allose kinase
Reads supporting (aligned to +/- strand):  ref base C (5/10);  new base T (32/32);  total (37/43)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

AGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTATTTTCGAG  >  NZ_LN832404/4303406‑4303969                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                     aGCGGCATTAAAGGCAGAAGAGGAGGCGGCAATAAAGCGCAGGACCGGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGGCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCa                                                                                                                                                                                                                                                                                     <  2:855893/290‑1 (MQ=255)    gcCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTTGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAgcg                                                                                                                                                                                                                                                                                  >  1:173229/1‑290 (MQ=255)    gcCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAgcg                                                                                                                                                                                                                                                                                  <  1:57207/290‑1 (MQ=255)       aTTAAAGTCAGAAGAGGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCa                                                                                                                                                                                                                                                                               <  1:1034152/290‑1 (MQ=255)               cAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGCACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCcgag                                                                                                                                                                                                                                                                       >  1:250891/1‑290 (MQ=255)                   agagGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAAt                                                                                                                                                                                                                                                                   <  1:65058/290‑1 (MQ=255)                   agaTGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAAt                                                                                                                                                                                                                                                                   >  1:687822/1‑290 (MQ=255)                    gaTGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCCTGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAAtt                                                                                                                                                                                                                                                                  >  1:494626/1‑290 (MQ=255)                      ggagagggaaaTAAAGAGCAGGCCCGGCTGGGGCATGGGACGTGGAAGGTACTTTTGGGTCATGGCAAGGAGAGTCGCGCGTGGGAAGGCGGGCAAATCCATACCGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTgg                                                                                                                                                                                                                                                                <  2:810581/281‑1 (MQ=255)                        gggcggcAATAAAGCGCACGACCGGATGCGGCAGGGGAGGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGtt                                                                                                                                                                                                                                                              <  2:149342/289‑1 (MQ=255)                               aaTAAAGAGCACGAGTGGATGCGGGAGGGGACGGTGCAGTGTGTATTGGGTGTGGGCAACGAGAGCCTGGCGTGGGAAGGCGTGAATATGCAGCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAAGGCTGGTGGCAATGGTCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGc                                                                                                                                                                                                                                                       <  2:695004/290‑1 (MQ=255)                                  aaaGCGCCCGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGTGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCAcc                                                                                                                                                                                                                                                    <  1:416748/290‑1 (MQ=255)                                   aggcgaaggACTTGATGCGGCAGTGGACGGCGAAGGTACTTTTGGGTCATGGCAACGAGGGGTGCGCGGGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCAccc                                                                                                                                                                                                                                                   <  2:914281/282‑1 (MQ=255)                                      cgcACGACCGGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAgg                                                                                                                                                                                                                                                <  1:975868/290‑1 (MQ=255)                                         aaggccTGATGGGGCAGGGGACGGCGCAGGTACGTTTGGGTCATGGCAACGAGAGGCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGAAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGAtt                                                                                                                                                                                                                                             <  2:570109/286‑1 (MQ=255)                                         aCGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCACGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑CAAGATCGCGCACTGGCTAATTTCGGGGCTGTTTTTCGGACCAGCGTCTTAGCCCCCTTCCAGCGCCATTGGTTTCGAGGCAccgcgcacg                                                                                                                                                                                                                                             >  2:82688/1‑283 (MQ=255)                                           gACCTGATGCGGCAGTGGACGGCGCAGGTACTTGTGGGGCATGGCAACGAGAGGCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGc                                                                                                                                                                                                                                           <  1:600979/290‑1 (MQ=255)                                            acctgtgggggCAGGGGACGGAGCAGGGACTTTTGGGTCAGGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTGTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGcc                                                                                                                                                                                                                                          <  2:687822/281‑1 (MQ=255)                                              cTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGcccc                                                                                                                                                                                                                                        >  1:953859/1‑288 (MQ=255)                                                gtggcgtgggTGGATGGGGCTGGTGCTTTGGGGTCCTGGCAACGCGAGGCTCGCGGTGGAAGTCTGTCATAGCCAGTACGCCACCTCCCAGGATCACCGCATCGGGAGCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCacac                                                                                                                                                                                                                                      <  2:616007/281‑1 (MQ=255)                                                 aTGCGCCAGTGGCCGGCGCAGGTACTTTTGGGTCATTGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCGCCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACAcg                                                                                                                                                                                                                                     <  1:732801/290‑1 (MQ=255)                                                     ggCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACCCgcgcc                                                                                                                                                                                                                                 >  1:971723/1‑289 (MQ=255)                                                     ggCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCa                                                                                                                                                                                                                                 <  1:283194/290‑1 (MQ=255)                                                       cAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCGGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAgt                                                                                                                                                                                                                               <  1:325138/290‑1 (MQ=255)                                                       cAGTGGACGGCGAAGGTACGTGTGGGTCATGGCAACTGTGGCCTCGCGGGGGAAGGCGGGCCTATCCATCACGCCACCGCCCAGGATCACCGCCTCGGGGTCGAACAGAGTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAgt                                                                                                                                                                                                                               <  2:464642/290‑1 (MQ=255)                                                              cGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGt                                                                                                                                                                                                                        <  1:377847/290‑1 (MQ=255)                                                               cgcggaaggacgtatGGGGCAAGGCAAGTAGAGGCTCGAGTGGGAAGGCAGGAAGATCCAGCAAGCCAACGCCCAGGATCACCGCATCGGGATCGAACAGAATAATTGTGGTGGAAAGGGCCCGTGCCGCGTGTGCAAGCAGACGCTGGACGAAAGGGGCGGTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTGCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTc                                                                                                                                                                                                                       <  2:293982/276‑1 (MQ=255)                                                                 cgcAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGCTTGCCACACGCGCAGTGTTGGGTCat                                                                                                                                                                                                                     >  1:967219/1‑290 (MQ=255)                                                                   cAGGTACTGTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCCAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCatat                                                                                                                                                                                                                   <  1:465356/290‑1 (MQ=255)                                                                    aGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑CAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCACTTGGTTTCCAGCCACCAAGGATGACCAACCGGCCATTGTTGGGTCatacc                                                                                                                                                                                                                  >  1:1064135/1‑288 (MQ=255)                                                                        tctcttggtgcATGGTAACGGGAGCCTCTCGTGGGACGGCGGGCATCGTCACCACGCCACCTCCCAGGATCACCGCATCGGGATCGAACAGATGAATGCTGGTGGCAATGGCCCGGGCCGCGTTTTCAAGCAGACGCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTccc                                                                                                                                                                                                              <  2:870954/280‑1 (MQ=255)                                                                                gTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGat                                                                                                                                                                                                      >  1:663149/1‑290 (MQ=255)                                                                                  cATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGatat                                                                                                                                                                                                    <  1:543172/290‑1 (MQ=255)                                                                                        aaCGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCCTTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACAAGCGCAGTGTTGGGTCATATCGCCCAGGGGGATATGACGCa                                                                                                                                                                                              >  1:270562/1‑290 (MQ=255)                                                                                            agagTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATAACCCAGTTc                                                                                                                                                                                          >  1:806654/1‑290 (MQ=255)                                                                                            agagTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTc                                                                                                                                                                                          <  1:187434/290‑1 (MQ=255)                                                                                                 ctcGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCACTGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCAATTCCACAGCAATTGGTTTCCAGGCCCCACGGCTTGCCCCACGCCCCGTGTGCAGTCCAATCCACACGGGGGATATGACCCCGtcagccag                                                                                                                                                                                     >  2:889847/1‑283 (MQ=255)                                                                                                         ggAAGGCGGGCATCTCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCg                                                                                                                                                                             <  1:222647/290‑1 (MQ=255)                                                                                                           aaGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGCGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCCGGGTTGGGTCATATCCCCCAGGGGGCTTGGACCACGTTCGCCTGCCCCACCgtg                                                                                                                                                                           >  2:386077/1‑290 (MQ=255)                                                                                                            aGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCCCACCgttt                                                                                                                                                                          >  1:91685/1‑288 (MQ=255)                                                                                                            aGGCGGGCAGATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCgtgt                                                                                                                                                                          <  1:831510/290‑1 (MQ=255)                                                                                                                 tgCAGATCCATAACGCCACCTCCCAGGATATCCGCTGCGGGATCGAACGAGTTAAGGCGGGTGGCAATGGCCGGGGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGTGCGGTGTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGCTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAAGTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCAcc                                                                                                                                                                     <  2:837607/289‑1 (MQ=255)                                                                                                                              ccccACCGCACAGGGGCACCGAAGCGGGAGCAAACAGATAAACGCGGGGGGCAGTGGCCCGGGCCGGTGTTGCAAGCAGATTAGGGACGAAAGGGGCGGTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGTTGTTGTTCGTACCAGCGGCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATGGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCa                                                                                                                                                        <  2:578845/288‑1 (MQ=255)                                                                                                                                ccACCGCCCAGGATCACCGCAGCGGGTTCGAACATGTTAATGCTGGTGGCAATGGCCGTGGCCGCGTGTTCAAGCAGACTCTGTACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCAcc                                                                                                                                                      <  2:170533/290‑1 (MQ=255)                                                                                                                                    ctcccAGGAGCACCGAAACGGTTTCGAAAATATGAATGCTGTGGGCAATGGCCCGGGCCGCGTGTTCAAGCAGAGTCGGGACGAAAGGGGCGTTTTCCGAATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGGACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTc                                                                                                                                                  <  2:329603/288‑1 (MQ=255)                                                                                                                                            aTCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGGTGGTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCAACCAGGATTGCCACACGCTCAGCGTTGGGGCATACCTCCCCGGGGGATAAGAACCAGGTCGCATGCAACACCGTGGGCAACCGGCACCGGCCGACCGGTCCTCcacaa                                                                                                                                          >  2:80411/1‑289 (MQ=255)                                                                                                                                              aaCCGAATCGGGATCAAACAGGTTAATTGGTGTGGAAAGGTCCCTGGAGGCGTTTTCAAGCAGACTGTGGACGAAAGGGTGGTGTGCCGCATGTACGA‑AAATATGGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑Tg                                                                                                                                       <  2:853892/289‑1 (MQ=255)                                                                                                                                                        ggATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCAt                                                                                                                             <  1:940169/290‑1 (MQ=255)                                                                                                                                                              aaCAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCCCCGTTCATCCACAC‑TGCGAACCCCATCCCCGt                                                                                                                       >  1:212493/1‑290 (MQ=255)                                                                                                                                                                cAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTTCCCCCGTCCACGGCTCACCGTTCACCCCCCC‑CGCCACCCCCCCCCCCGTAc                                                                                                                     >  2:34605/1‑290 (MQ=255)                                                                                                                                                                    ttAATGCTGGTGGCAAGGGCCCGTGCCTCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCgag                                                                                                                 <  1:664952/290‑1 (MQ=255)                                                                                                                                                                               ggCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGACATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCAAACCGTGTGCACCCGTACACGGCGCACCGGTCATCC‑CACATGCGAACCCCATCCCCGCACCGAGAAAGGCCGCCa                                                                                                      >  1:954106/1‑290 (MQ=255)                                                                                                                                                                                gCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACACAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGAGCACCGTTCAACCAAAC‑TGCAAACCCCATCCCCGTACCGAGGTAGGCAGCCAg                                                                                                     >  1:841704/1‑290 (MQ=255)                                                                                                                                                                                 cAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGa                                                                                                    <  1:208672/290‑1 (MQ=255)                                                                                                                                                                                    tGGCCCGTTCCGCGTTTTCAAGCAGAGTCTGGACGAAAGGGGCGTTTTCCGCATGGACTA‑AAAGATCGCGCAATGGTTAATTTCGGGGCTGGTGTTCGTACCAGCGTCTTAGCGCCATTCCAGCGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAAcc                                                                                                 <  1:1024122/290‑1 (MQ=255)                                                                                                                                                                                          gTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCATGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACAAgttgt                                                                                           >  1:929287/1‑290 (MQ=255)                                                                                                                                                                                             ccGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCCCCGTTCATCCACAC‑TGCGAACCCCATCCCAGTACCGAGATAGGCCGCCAGAACCAGTTGTTGc                                                                                        >  1:1030463/1‑290 (MQ=255)                                                                                                                                                                                                cgTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTa                                                                                     <  1:265344/290‑1 (MQ=255)                                                                                                                                                                                                       ttagaGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGt                                                                              <  1:1050059/286‑1 (MQ=255)                                                                                                                                                                                                       aaGCAGACGTGGGTCGAATGTGGCGGGTGCCGCATGG‑CGACAAAGATCGCGCAAGTGGTAGTTTCGGGGCTGTTGGTCGTACCAGCGTCTTAGCGCCATTCCAGTGCAATTGGTGTCCAGGCACACAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGt                                                                              <  2:250891/290‑1 (MQ=255)                                                                                                                                                                                                        aGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGtt                                                                             >  1:612796/1‑290 (MQ=255)                                                                                                                                                                                                          cAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCCGAACCAGTTGTTGCATAAGGCGGtttt                                                                           >  1:236358/1‑290 (MQ=255)                                                                                                                                                                                                             aCTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTcta                                                                        >  1:834970/1‑290 (MQ=255)                                                                                                                                                                                                                 tGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGCGCTAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCtactac                                                                    >  1:123297/1‑290 (MQ=255)                                                                                                                                                                                                                   gACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGCTAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGt                                                                  >  1:671948/1‑290 (MQ=255)                                                                                                                                                                                                                         aGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTGCTACTACGTCCCAgg                                                            >  1:78980/1‑290 (MQ=255)                                                                                                                                                                                                                          ggggCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGCCCCAGTTCGCCTGCCACCCCGTGTGCCCCCGTCCCCGGCCCACCGTTCATCCACCC‑TCCGACCCCCCCCCCCGTCCCCCGATCGGCCGCCCGACCCCGTTGTTGCGTCACGCGGCTTTCCACTCTGTccctcgc                                                           >  2:521296/1‑286 (MQ=255)                                                                                                                                                                                                                           gggAGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCCGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGgag                                                          >  1:489160/1‑290 (MQ=255)                                                                                                                                                                                                                               gTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGtt                                                      <  1:678537/290‑1 (MQ=255)                                                                                                                                                                                                                                ttttCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTg                                                     <  1:272616/290‑1 (MQ=255)                                                                                                                                                                                                                                          ggACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTCAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACg                                           >  1:156517/1‑290 (MQ=255)                                                                                                                                                                                                                                              gA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATCGGCCGCCAGAACCAGTTGTTGCGTATGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTcgc                                       >  1:677437/1‑290 (MQ=255)                                                                                                                                                                                                                                              gA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGCCCCAGGATAGTTGCAGGTTAACGTcgc                                       >  1:443434/1‑290 (MQ=255)                                                                                                                                                                                                                                                    gATCTCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACCCCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTAGAGGGaa                                  >  1:721928/1‑290 (MQ=255)                                                                                                                                                                                                                                                     aTCGCGCAATGTGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGaaa                                 <  1:420520/290‑1 (MQ=255)                                                                                                                                                                                                                                                       cgcgCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAAc                               >  1:278192/1‑290 (MQ=255)                                                                                                                                                                                                                                                              tGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCg                        <  1:939180/290‑1 (MQ=255)                                                                                                                                                                                                                                                               cggTAATGCCGGTGGTGTTGTTCGTACCAGCGTCTTAGCGCCATGCCGGGTCAATTGGGTTCCAGTCACCCAGGATTGCCCCACGAGCAGTGTTGTGTCATATCCCCCAGGTGGCTATGACCCAGTTCGCCTGCCAAACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCgg                       <  2:191845/289‑1 (MQ=255)                                                                                                                                                                                                                                                                    aTTTCGGGGCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGTACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAAt                  >  1:318878/1‑290 (MQ=255)                                                                                                                                                                                                                                                                     tttCGGGGTTGGTTGTCGTAACGTCGTCTTGGCGCCATTCCAGGTCAATTGGTTTCCCGGCCCCCAGGTTTGCAACAGGCGCAGTTGTGGGTCATACCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAAtt                 <  2:967219/290‑1 (MQ=255)                                                                                                                                                                                                                                                                           ggCTGTTGTTCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCAAACGCGCAGTGTTGGGTCATATATCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGGGGGCACCCGTCCACGGCGCAACGTTCATCCACAC‑TGCGAACCCACTCACAGTACCGAGCTAGGCAGACGGCACAAGTTGATGCGAAAGGCGGTGTTCCACTCGGTACCGGGAGGGTAGACGGTAAACAGGACGGGGAAACACAAccagcaaatgcaagtg           >  2:1002885/1‑276 (MQ=255)                                                                                                                                                                                                                                                                             cTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCAACCCGTGTGCACCCGTCCACGGCGCCCCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCCACTACGTCCCAGGAGCGTTGCAGGTTAACGCCGCGGGAAAACTCACTCGGACACTTCAGCGTag         >  2:449727/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                   ttctgaCCAGCGTCTGAGCGCCATGCCAGAGCAATTGGTTGTCAGGCACCCAGGATTGCCACACGCTCATTGTGGGGTCATATCTCCCAGGGGTATATGACCCAGTTCGCCTGCCACACCGTTTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTATTTTCGa   <  1:604088/285‑1 (MQ=255)                                                                                                                                                                                                                                                                                    tCGTACCAGCGTCTTAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTATTTTCGAg  <  1:654177/290‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                     AGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATTCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGA‑AAAGATCGCGCAATGGGTAATTTCGGGGCTGTTGTTCGTACCAGCGTCTCAGCGCCATTCCAGAGCAATTGGTTTCCAGGCACCCAGGATTGCCACACGCGCAGTGTTGGGTCATATCTCCCAGGGGGATATGACCCAGTTCGCCTGCCACACCGTGTGCACCCGTCCACGGCGCACCGTTCATCCACAC‑TGCGAACCCCATCCCCGTACCGAGATAGGCCGCCAGAACCAGTTGTTGCGTAAGGCGGTTTTCTACTACGTCCCAGGAGAGTTGCAGGTTAACGTCGCGGGAAAACTCAACCGGACAATTCAGCGTATTTTCGAG  >  NZ_LN832404/4303406‑4303969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑