Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 4,471,308 A→G 79.3% intergenic (+117/‑28) EK12AG100_RS22100 → / → EK12AG100_RS22105 TetR/AcrR family transcriptional regulator/YhcH/YjgK/YiaL family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324044,471,3080AG79.3% 241.4 / 64.5 116intergenic (+117/‑28)EK12AG100_RS22100/EK12AG100_RS22105TetR/AcrR family transcriptional regulator/YhcH/YjgK/YiaL family protein
Reads supporting (aligned to +/- strand):  ref base A (11/13);  new base G (37/55);  total (48/68)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.21e-01

Only 100 of 127 total aligned reads displayed.

GACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTG  >  NZ_LN832404/4471024‑4471594
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                              
gACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCgttg                                                                                                                                                                                                                                                                                           >  1:941574/1‑290 (MQ=255)
   tACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGttt                                                                                                                                                                                                                                                                                        <  1:118850/290‑1 (MQ=255)
        cGCCAGGCCGCAGCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTttaatt                                                                                                                                                                                                                                                                                   <  1:26868/290‑1 (MQ=255)
          ccAGGCGGGATCCACAACGGGCACAATGGGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGGGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTttaattaa                                                                                                                                                                                                                                                                                 <  2:793825/290‑1 (MQ=255)
            aGGGGGCATCCACAACGGGGACATGGGGTTAATGAGTTTATGAGTACCGGGATGTCTGGGCTTGCTGCGGAGGAACGAGAGGGGCAGGTTATAGAACAACGCTGTGCAAAAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAAAGAAACGTTGTTTTAATTAAgg                                                                                                                                                                                                                                                                               <  2:663266/290‑1 (MQ=255)
             ggcggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGa                                                                                                                                                                                                                                                                              <  1:143146/290‑1 (MQ=255)
              gcggcATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTTCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAg                                                                                                                                                                                                                                                                             >  1:207626/1‑290 (MQ=255)
                   aTCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATACGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGCTTATCTTTTCGTATAACGATAGGAATGAAACGTTTGTATAATTAAGGTATGGaa                                                                                                                                                                                                                                                                        >  2:374738/1‑290 (MQ=255)
                     ccACAACGCGCACGATGTGGTACTGATTTTTAGAGTACCGTGATGTCTGGGCTTTCTTCGAAGGCACGTGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGTAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGaaaa                                                                                                                                                                                                                                                                      <  2:886333/290‑1 (MQ=255)
                        caACGGGCACAAGGGGGGGCTGATGTTATGGGGACCGTGATGTCTGGGGTTGCTGGGAAGGCACGGGAGGGGCACTCTATAGAAAAACTCGGTGCAACAGCTGCAAGGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgat                                                                                                                                                                                                                                                                   <  2:5001/290‑1 (MQ=255)
                        aaaagcgacaaaTGTGTGACGGGATTTAGGAGGACCGTGATTGCTGGGCTTTCTGGGAAGGCACGAGAGGGGCACTCGATAGAACAACTCTGTGCAACAGCGGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAgat                                                                                                                                                                                                                                                                   <  2:742993/280‑1 (MQ=255)
                          aCGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACCATAATTCCAGCGTTAAGGCCAGCGGAATTGATTATCTTTTCGTTTATAGTAAGAATTGCCGCGTTGTTTTAATTAATGCTTGGAAAAgatgc                                                                                                                                                                                                                                                                 >  2:538201/1‑289 (MQ=255)
                                     gtgttAGTGATTTTAGGAGTACCGGGATGTCTGGGATTTCTGCGAAGGCACGAGAGGGGCAATCTAGAGAACAACGCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAat                                                                                                                                                                                                                                                      <  2:1005970/290‑1 (MQ=255)
                                         gagtggttgtATGAGTACCGGGATGTCTGGTCTTTCTGCGAAGGCACGAGAGGGGCAATCTATAGAACAACTCTGGGCAACAGCGGCAATGGCGGGGGAAGCGATAAAAACTAGTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTc                                                                                                                                                                                                                                                  <  2:776341/281‑1 (MQ=255)
                                           cTGATTTGATGAGTACCGTGAGGTCTGGGAGTTCTGCGAAGGCACGAGAGGGGCAAGCTAAAGAACAACGCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCAt                                                                                                                                                                                                                                                <  2:494906/290‑1 (MQ=255)
                                              aTTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGCAAAGTTGTGATGAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATATTTTCGTATAACGATAGAAATGAAGCGTTGTTTAAGTTAAGGAGTGGAAAAGGTGATCATCGGAAATATTCATaag                                                                                                                                                                                                                                             >  2:233732/1‑289 (MQ=255)
                                                  tATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGGTCATCGGAAATATTCATAATCTTc                                                                                                                                                                                                                                         >  2:1036853/1‑290 (MQ=255)
                                                  tATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTc                                                                                                                                                                                                                                         <  1:535253/290‑1 (MQ=255)
                                                   aggatgaCCGTGATGTCTGGGCTTTCGGCGAAGGAACGAGAGTGGCACTCGATAGAACAACTCTGGGCAAAAGCGGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCa                                                                                                                                                                                                                                        <  2:733998/284‑1 (MQ=255)
                                                     gggTACGGTGATGTCTGGGCTTTCTGCGAAGGTACGATAGGGGCACTCTATATAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGc                                                                                                                                                                                                                                      <  2:30775/288‑1 (MQ=255)
                                                     gAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATAGGAAATATTCATAATCTTCAGc                                                                                                                                                                                                                                      >  2:86731/1‑290 (MQ=255)
                                                      ggtaacggtaTGTCTGGGCGTTGGGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGcc                                                                                                                                                                                                                                     <  2:672060/281‑1 (MQ=255)
                                                      aGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGAGGAAGTGATTAACTTTTAGTATAACGATATGAATAGAGCGTTGGTTTAATTAAGGAATGGAAAAAGTGATAATAGGAAAATTTCATACACATCAGcc                                                                                                                                                                                                                                     >  2:397232/1‑290 (MQ=255)
                                                          ccGGGATGTATGGGCTGTCTGGGAAGGCACTGTAGGGGCATCCTATAGAACAACTTTGTGCAACAGCGGGAAGGGCGTGGGAAGCGATAAAAACGATTCTTGAGGAGTGAGGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTgg                                                                                                                                                                                                                                 <  2:1011310/290‑1 (MQ=255)
                                                            gTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCt                                                                                                                                                                                                                               <  1:17049/290‑1 (MQ=255)
                                                              gATGTCTGGGCTTTCTGCGAAGGCACGAGATGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAc                                                                                                                                                                                                                             <  1:822099/290‑1 (MQ=255)
                                                                 gTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATACGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGACGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTAcccc                                                                                                                                                                                                                          >  1:750074/1‑290 (MQ=255)
                                                                        cTTTCGGCGAAGGAAGAAGGGTGGCACTTTATAGAAAAACTCGGGGCAACAGGGGCAATGGCGGTGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTGGTGATAAGACAATAATTCACGCGGTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGtt                                                                                                                                                                                                                   <  2:686123/290‑1 (MQ=255)
                                                                              gCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCa                                                                                                                                                                                                             >  1:911040/1‑290 (MQ=255)
                                                                              gCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCa                                                                                                                                                                                                             <  1:344002/290‑1 (MQ=255)
                                                                                  aGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTATAATTAAAGAGTGGAAAAGATGATACTAGGAAATATTCATAATATTCCAACGTGGGTAAACCAGGCGTTCCGCCAgcag                                                                                                                                                                                                         >  2:907293/1‑287 (MQ=255)
                                                                                     cACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGAtt                                                                                                                                                                                                      >  1:589194/1‑290 (MQ=255)
                                                                                     cACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGGGGAAAAGATGATCATCGGAAATATTCATACTCTTCAACCGTGGCTACCCCAGGAGtactgcaaggagatg                                                                                                                                                                                                      >  1:250331/1‑276 (MQ=255)
                                                                                        gagaGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGCAATGAAGCGTTGTTTTAATTAAGGAGTTGAAAAGATGATCATCGGAAATATTTCAAATCTTCAGCCTTGGCTACCCCCGGCGTTACGCCAGGCGATTGAg                                                                                                                                                                                                   >  2:823198/1‑290 (MQ=255)
                                                                                           agGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCat                                                                                                                                                                                                <  1:830086/290‑1 (MQ=255)
                                                                                            ggggCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCata                                                                                                                                                                                               <  1:76490/290‑1 (MQ=255)
                                                                                                  cgctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGAGGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAg                                                                                                                                                                                         <  2:292218/288‑1 (MQ=255)
                                                                                                      ataGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCTGGCGATTGAGCATAACAAGGcaca                                                                                                                                                                                     >  2:301081/1‑290 (MQ=255)
                                                                                                       taGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCAACGGAAATATTCATAATCTTCAGCCGTGGCTAACCCACGAATTACGCCAGGCGAATGAGCAACTAAAAGCacac                                                                                                                                                                                    >  2:765997/1‑290 (MQ=255)
                                                                                                       taGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCaaac                                                                                                                                                                                    >  1:1037684/1‑290 (MQ=255)
                                                                                                          atcaacGCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGtt                                                                                                                                                                                 <  1:719611/288‑1 (MQ=255)
                                                                                                          aacaacTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGtt                                                                                                                                                                                 >  1:523105/1‑290 (MQ=255)
                                                                                                              acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACATGTTACgg                                                                                                                                                                             <  1:616107/290‑1 (MQ=255)
                                                                                                                  tgtgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGa                                                                                                                                                                         <  1:1055722/290‑1 (MQ=255)
                                                                                                                        aCAGCTGCAGTGGCGGGGGAAGCGATAAAAACTATTCTTGGGGAGTGAGTCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATGCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGcc                                                                                                                                                                   <  2:691705/290‑1 (MQ=255)
                                                                                                                          aGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCAGTTGAGCATATCAAAGCACACGTTGAGGCAGAAAccgcaa                                                                                                                                                                 >  2:118718/1‑285 (MQ=255)
                                                                                                                           gCTGCAATGTGGGGGGAAGCGATAAAAACTATTATTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTACGTATAACGATAGAAATGAAGCGTTGGTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATACATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCaaa                                                                                                                                                                <  2:493849/290‑1 (MQ=255)
                                                                                                                             ggaaagggCGGGGGAAGCGAGAAAAACTATGCTGGGGGAGTGATGGGTGCCTTGACCCGAAAGGCGGGAAAGGGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAgg                                                                                                                                                              <  2:639657/284‑1 (MQ=255)
                                                                                                                                aaTGGTGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAGGGGCa                                                                                                                                                           >  1:412547/1‑290 (MQ=255)
                                                                                                                                aaTGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCa                                                                                                                                                           <  1:187447/290‑1 (MQ=255)
                                                                                                                                      gggggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACg                                                                                                                                                     >  1:120813/1‑290 (MQ=255)
                                                                                                                                           aaGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATc                                                                                                                                                <  1:86731/290‑1 (MQ=255)
                                                                                                                                             gagATAAAAACTATTGTTGAGGGGTGATGCGGGCCTGGACCCGAAAGGCGGGAAAGGGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGa                                                                                                                                              <  2:941574/288‑1 (MQ=255)
                                                                                                                                               gttAAAAACTATCCTTGAGGAGGGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGAAAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAg                                                                                                                                            <  2:202356/288‑1 (MQ=255)
                                                                                                                                               gATAAAAACATGTCTTGAGTATTGAGGCGGGCCTTGACCCGAAAGGCGGGAAAGAGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGTCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAg                                                                                                                                            <  1:924650/290‑1 (MQ=255)
                                                                                                                                                  aaaaaCTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAACGGGCAAGCACGATAACGacagca                                                                                                                                         >  2:113717/1‑285 (MQ=255)
                                                                                                                                                  aaaaaCTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATCTCGAAGGCa                                                                                                                                         >  1:728347/1‑290 (MQ=255)
                                                                                                                                                     aaCTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATc                                                                                                                                      <  1:714986/290‑1 (MQ=255)
                                                                                                                                                              tGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATCTTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAACCGCCAACGGGCAAGCAAGAAAACGACGGCAATCGACtcgttt                                                                                                                             >  2:844097/1‑285 (MQ=255)
                                                                                                                                                                   agTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATCTCAAAGCACACGTTACGGCAGAAACCCCAAAGGGCCAGCCCGCTACCGAAGGCAATCCACTGGTttatcct                                                                                                                        >  2:157703/1‑288 (MQ=255)
                                                                                                                                                                          cGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCgg                                                                                                                 <  1:563474/290‑1 (MQ=255)
                                                                                                                                                                               cTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTGGTGATAAGACAATAATTAACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGat                                                                                                            <  2:174733/290‑1 (MQ=255)
                                                                                                                                                                                ttGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTATCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGata                                                                                                           <  1:408397/290‑1 (MQ=255)
                                                                                                                                                                                     ccGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGAcc                                                                                                      <  1:804246/290‑1 (MQ=255)
                                                                                                                                                                                       gAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGa                                                                                                    <  1:602307/290‑1 (MQ=255)
                                                                                                                                                                                          aagCGGGAAAGCTCCTTCCGATAATTTGTTATAAGACAATAATTCACGCGTTAAGGCTAGCTGAATTGATTATCTTTTCGTATAACGATATAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTTGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGcc                                                                                                 <  1:323009/288‑1 (MQ=255)
                                                                                                                                                                                          aGGGGGGAAAGGGCCTGCCGATAAGTTGGGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAAGTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGcc                                                                                                 <  2:82256/290‑1 (MQ=255)
                                                                                                                                                                                           ggCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCg                                                                                                <  1:168086/290‑1 (MQ=255)
                                                                                                                                                                                           ggCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGAAACCGAAGGCAAACGCAGGTTTTAGCTTATCTCGGAAGATGTGACCGaacag                                                                                                >  2:831989/1‑286 (MQ=255)
                                                                                                                                                                                            gCGGGAAAGCGCCGGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGt                                                                                               <  1:110629/290‑1 (MQ=255)
                                                                                                                                                                                                 aaaGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCCCGATCTCGAAGGAAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAACCGTACGac                                                                                          >  2:772975/1‑289 (MQ=255)
                                                                                                                                                                                                   agtgccggccgGTAAGTTGTGATAAGAAAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGc                                                                                        <  2:382543/287‑1 (MQ=255)
                                                                                                                                                                                                        ctgctgATAAGTTGTGATAAGACAATAATTCACGAGTTAAGGATAGCGGAATGGATTATCGTTTCGTATAACGATAGAAATGGAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGAAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGcc                                                                                   <  1:321880/290‑1 (MQ=255)
                                                                                                                                                                                                             gATAAGTTGTTATAATACCATAATTCACGCGTTAAGGCTAACGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCg                                                                              <  2:377502/290‑1 (MQ=255)
                                                                                                                                                                                                                  gTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTAAGGCAGAAAAGCCAAAGGGCAAGCACGATATCGAAGGCAAAGGACTGTTTTATCCTATCTCGGAAGATATGAACAAGCCGTACGAAGATCGACGTGCGGAGTa                                                                         >  2:139925/1‑290 (MQ=255)
                                                                                                                                                                                                                       gATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCCGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGCAGATATGACCGAGCCGTACGACGCTCGCCGTGCGGAGTCCCATg                                                                    >  2:878801/1‑290 (MQ=255)
                                                                                                                                                                                                                       gATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATg                                                                    <  2:44535/290‑1 (MQ=255)
                                                                                                                                                                                                                          aaGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACTGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGccc                                                                 >  1:387353/1‑290 (MQ=255)
                                                                                                                                                                                                                            gACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAACGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCCCGGACGATATGAACAAGACGTACGCAGCTCGCCGTGCGCAATACCATGCCCGc                                                               >  2:859379/1‑290 (MQ=255)
                                                                                                                                                                                                                             aCAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGGTCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCt                                                              <  1:1036853/290‑1 (MQ=255)
                                                                                                                                                                                                                             aCAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTTTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCt                                                              <  1:304619/290‑1 (MQ=255)
                                                                                                                                                                                                                              cAATAATTCACGCGTTAAGGCTTGCTGAATTGATTAGCTTTTCGTTTAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGTTGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTa                                                             <  2:453930/290‑1 (MQ=255)
                                                                                                                                                                                                                               aataatTCCAGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAAGTAAGGAGTGGAAAAGATGATCATCGGAAATAGTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTAt                                                            <  2:211632/290‑1 (MQ=255)
                                                                                                                                                                                                                                    ttCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAACCGTACGAAGCTCGCCGTTCGGAGTACCATGCCCGCTTTCTTGa                                                       >  1:963872/1‑290 (MQ=255)
                                                                                                                                                                                                                                            ttAAGGCGAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGa                                               <  1:633259/290‑1 (MQ=255)
                                                                                                                                                                                                                                                ggCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATtgc                                           <  1:148102/290‑2 (MQ=255)
                                                                                                                                                                                                                                                    atcGGAATTTATTCTCTGTTCGTATCACTATAGAAATGAAGCGTTGTCTGTATTAAGAAGTGGAAAATATGAGCATCGCAAATATTCATAATCTTCAGCCGTGGGTACCGCGGGAGTTACGCCAGGCGTTTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTa                                       <  2:956839/288‑1 (MQ=255)
                                                                                                                                                                                                                                                      cGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTaaa                                     <  1:735089/290‑1 (MQ=255)
                                                                                                                                                                                                                                                        gAATTGATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAg                                   <  1:374738/290‑1 (MQ=255)
                                                                                                                                                                                                                                                        gAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAATCTCGACGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAg                                   >  1:390534/1‑290 (MQ=255)
                                                                                                                                                                                                                                                         aatgtgttaacgtttGGTATAACGTGATAAATGATACGTGGTTTTAAGTAGTGGTTGTGAAGGAGGATCATCCGAAATATACAGAAACTTCAGCCACGGGTATCTCAGGAGTTACGCCAGGCGATTGAGCATAGCCAAGCACACGTTACGGCGGTAACGCGAAAGGGCGAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAgg                                  <  2:579760/278‑1 (MQ=255)
                                                                                                                                                                                                                                                             gATTATCTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAACCGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTTCGGAGTACCCTGCCCGCTATCTTGACATTCCGCTTGTGTTAAACGGTcga                              >  2:474835/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                   cTTTTCGTATAACGATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCACGGAAGATATGACCGAGCCGTACGAAGATCGACGTGCGGAGTCACATTCACGCTCTCATGACATTCCGATTGTGTTAAACAGTAAGGACGGc                        >  2:578176/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                       tCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGAAATTCCTATTTTGTTAAAAGGTCATCAAGGCAtat                    >  2:436074/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                            taaccgtAGAAATGAAGCGTTGTTTTAATTACGGGGTGGAAAAGATGTTCATCGGAAATTTTCATAATCTTCCGCCGTGGCTACCCCAGGGGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTc               <  2:589194/284‑1 (MQ=255)
                                                                                                                                                                                                                                                                                gATAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCa           <  1:772975/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 aTAGAAATGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCAc          >  1:241280/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                      aaTGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAAc     >  1:300456/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                       aTGAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGAACCATGCCCGCTATCTTGACATTCAGCTTGTGTTAAAAGGTCAGGAAGGCATGACATTCAGCACGCacac    >  1:543363/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                                         gAAGCGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTg  >  1:452736/1‑290 (MQ=255)
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                              
GACTACATCGCCAGGCGGCATCCACAACGCGCACAATGTGTGACTGATTTTATGAGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGAGTGGAAAAGATGATCATCGGAAATATTCATAATCTTCAGCCGTGGCTACCCCAGGAGTTACGCCAGGCGATTGAGCATATCAAAGCACACGTTACGGCAGAAACGCCAAAGGGCAAGCACGATATCGAAGGCAATCGACTGTTTTATCTTATCTCGGAAGATATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTG  >  NZ_LN832404/4471024‑4471594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: