Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	4,023,931	A→T	46.3%	intergenic (+230/+151)	EK12AG100_RS19960 → / ← fadA	NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	4,023,931	0	A	T	46.3%	19.2 / 34.2	28	intergenic (+230/+151)	EK12AG100_RS19960/fadA	NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA
Reads supporting (aligned to +/- strand):  ref base A (8/7);  new base T (11/2);  total (19/9)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

CGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCA  >  NZ_LN832404/4023669‑4024224                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        cGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGtt                                                                                                                                                                                                                                                                         <  1:72316/294‑1 (MQ=18)     tGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTGTCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcg                                                                                                                                                                                                                                                                     >  1:591008/1‑294 (MQ=18)         tGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcgccgc                                                                                                                                                                                                                                                                 >  1:74365/1‑294 (MQ=18)          ggCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCa                                                                                                                                                                                                                                                                <  1:419790/294‑1 (MQ=18)           gCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACCAATAGTCCGAACCGTAGGCCGGATAAGCCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGCAGACCGGATACGGCGTTCACGCCGCAt                                                                                                                                                                                                                                                               >  1:173220/1‑294 (MQ=18)                gCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                                                                                                                                                          >  1:757098/1‑294 (MQ=17)                   tttGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGACTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGCCCGGCTAAGGCGTCCACGCTGCATCCGGCaag                                                                                                                                                                                                                                                       >  1:50528/1‑293 (MQ=17)                            aTCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGttaaac                                                                                                                                                                                                                                              >  1:896115/1‑289 (MQ=17)                               tGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGCTAAGGCGTTCACGCCGCATCCGGCAAGTGGttaaacccg                                                                                                                                                                                                                                           >  1:224694/1‑286 (MQ=14)                               tGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTACTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGCTCAGGCGTTCACGCCGCATCCGGCAAGTGGttaaacccg                                                                                                                                                                                                                                           >  1:300035/1‑286 (MQ=14)                                gagaTATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCCTCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGCACGGTAGCCCGGACAAGGCGTTCCCGCCGCATCCGGCAAGTggctacacccgt                                                                                                                                                                                                                                          >  2:483878/1‑284 (MQ=17)                                    tataAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACCAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTCTCAGGCCTAC‑ATTGGTGCCGGATCGGTCGCCCGGATCAGGCGTTCACCCCCCATCCGGCCAGTGGttaaacccgctcac                                                                                                                                                                                                                                      >  2:689046/1‑281 (MQ=14)                                    tataAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGttaaccccgctcaa                                                                                                                                                                                                                                      >  1:130427/1‑281 (MQ=14)                                                                                                                                                                                                                   atctggcaaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACAACGTCGCAATACCCTGACCCAGAACGATACACATCGTCGCCAGCCCAAACTACACGTCTTTGCGTTCCATCAGa                                                      >  1:666810/13‑294 (MQ=21)                                                                                                                                                                                                                         caaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCCCGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCCATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAACCTGAACGTCTTTGCTTCCCATCACATTcacc                                                >  2:417520/7‑292 (MQ=255)                                                                                                                                                                                                                          aaaccatgCCCGAGTCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTcagca                                               <  1:980872/289‑1 (MQ=255)                                                                                                                                                                                                                               attcccgATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCAGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCgtg                                          <  1:986336/288‑1 (MQ=255)                                                                                                                                                                                                                                     gATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGCCCAAACTGCACGTCCTTTCGTTCCATCCGATTCAGCCGCGTGGTGCTg                                    >  2:170813/1‑294 (MQ=255)                                                                                                                                                                                                                                      aTGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCTTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCCCAAACACCGTCGCCATACCCCGACCCAGACCGATACCAACCGCCGCCCGACCAAACTGAACGCCATTGCGTTCCTTCACATTCCGCCGCGTGGCGCTGa                                   >  2:330600/1‑294 (MQ=21)                                                                                                                                                                                                                                       tGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGACCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGCCGCACTACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACAGAACGTCATTGCCTTCCATCAGATTCACCCGCGTGCTGCCGAt                                  >  2:128163/1‑294 (MQ=25)                                                                                                                                                                                                                                        gCGACGCTGTCGCGTCTGATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATa                                 <  1:915003/294‑1 (MQ=255)                                                                                                                                                                                                                                          gaggCTGTTGCGTTTTATCGGGCCTACAAATAGT‑CC‑GAACCGGAGGCCGGATAAGGCGTTTACGCCGCATCAGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATAcg                               <  2:510248/291‑1 (MQ=255)                                                                                                                                                                                                                                           aCGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCACATTCAACAGCgtgggtcgtctacgc                              >  2:854816/1‑283 (MQ=255)                                                                                                                                                                                                                                             gCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACgcgc                            <  1:307763/294‑1 (MQ=255)                                                                                                                                                                                                                                                  cgcgTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCgg                       <  1:746721/294‑1 (MQ=255)                                                                                                                                                                                                                                                  agcgTCTTAGCGGGCGTACAAAGAGG‑CC‑GAACCGAGGGCGGGATAAGTGTGTGAGGGCGAATCCGGGAATGTGTGCATGATGCCTGATGGGACGCTGGCGCGTCGTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTGCACGCCGCAGCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCGTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCgg                       <  2:1078348/293‑1 (MQ=255)                                                                                                                                                                                                                                                         tATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGCCCAAACTGAACGTCTTTGCGTTCCCTCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGACCAAcc                >  1:556579/1‑294 (MQ=255)                                                                                                                                                                                                                                                           tCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCa              <  1:1010096/294‑1 (MQ=255)                                                                                                                                                                                                                                                              tGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCg           >  1:188962/1‑294 (MQ=255)                                                                                                                                                                                                                                                                  tACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATg       <  1:619495/294‑1 (MQ=255)                                                                                                                                                                                                                                                                   ccAAGTAGG‑CC‑GAACCGGAGCCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGa      <  2:304621/293‑1 (MQ=255)                                                                                                                                                                                                                                                                      caTAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTGTACGCCGCATCCGGCAATTGGTGCAGGATGCCTGATGCGACGCTGGCGCGTCTGATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGAccc   <  1:611470/293‑1 (MQ=255)                                                                                                                                                                                                                                                                       aTAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCa  >  1:691045/1‑294 (MQ=255)                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        CGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCA  >  NZ_LN832404/4023669‑4024224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑