Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_LN832404 4,023,940 A→T 39.2% intergenic (+239/+142) EK12AG100_RS19960 → / ← fadA NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_LN8324044,023,9400AT39.2% 29.7 / 25.8 28intergenic (+239/+142)EK12AG100_RS19960/fadANAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA
Reads supporting (aligned to +/- strand):  ref base A (9/8);  new base T (9/2);  total (18/10)
Fisher's exact test for biased strand distribution p-value = 2.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAGCGCGATC  >  NZ_LN832404/4023669‑4024232
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                    
cGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGtt                                                                                                                                                                                                                                                                                 <  1:72316/294‑1 (MQ=18)
    tGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTGTCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcg                                                                                                                                                                                                                                                                             >  1:591008/1‑294 (MQ=18)
        tGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCAcgccgc                                                                                                                                                                                                                                                                         >  1:74365/1‑294 (MQ=18)
         ggCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCa                                                                                                                                                                                                                                                                        <  1:419790/294‑1 (MQ=18)
          gCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACCAATAGTCCGAACCGTAGGCCGGATAAGCCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGCAGACCGGATACGGCGTTCACGCCGCAt                                                                                                                                                                                                                                                                       >  1:173220/1‑294 (MQ=18)
               gCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                                                                                                                                                                                  >  1:757098/1‑294 (MQ=17)
                  tttGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGACTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGCCCGGCTAAGGCGTCCACGCTGCATCCGGCaag                                                                                                                                                                                                                                                               >  1:50528/1‑293 (MQ=17)
                           aTCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGttaaac                                                                                                                                                                                                                                                      >  1:896115/1‑289 (MQ=17)
                              tGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGCTAAGGCGTTCACGCCGCATCCGGCAAGTGGttaaacccg                                                                                                                                                                                                                                                   >  1:224694/1‑286 (MQ=14)
                              tGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTACTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGCTCAGGCGTTCACGCCGCATCCGGCAAGTGGttaaacccg                                                                                                                                                                                                                                                   >  1:300035/1‑286 (MQ=14)
                               gagaTATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCCTCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGCACGGTAGCCCGGACAAGGCGTTCCCGCCGCATCCGGCAAGTggctacacccgt                                                                                                                                                                                                                                                  >  2:483878/1‑284 (MQ=17)
                                   tataAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACCAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTCTCAGGCCTAC‑ATTGGTGCCGGATCGGTCGCCCGGATCAGGCGTTCACCCCCCATCCGGCCAGTGGttaaacccgctcac                                                                                                                                                                                                                                              >  2:689046/1‑281 (MQ=14)
                                   tataAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAC‑ATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGttaaccccgctcaa                                                                                                                                                                                                                                              >  1:130427/1‑281 (MQ=14)
                                                                                                                                                                                                                  atctggcaaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACAACGTCGCAATACCCTGACCCAGAACGATACACATCGTCGCCAGCCCAAACTACACGTCTTTGCGTTCCATCAGa                                                              >  1:666810/13‑294 (MQ=21)
                                                                                                                                                                                                                        caaaccatgCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCCCGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCCATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAACCTGAACGTCTTTGCTTCCCATCACATTcacc                                                        >  2:417520/7‑292 (MQ=255)
                                                                                                                                                                                                                         aaaccatgCCCGAGTCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTcagca                                                       <  1:980872/289‑1 (MQ=255)
                                                                                                                                                                                                                              attcccgATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCAGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCgtg                                                  <  1:986336/288‑1 (MQ=255)
                                                                                                                                                                                                                                    gATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGCCCAAACTGCACGTCCTTTCGTTCCATCCGATTCAGCCGCGTGGTGCTg                                            >  2:170813/1‑294 (MQ=255)
                                                                                                                                                                                                                                     aTGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCTTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCCCAAACACCGTCGCCATACCCCGACCCAGACCGATACCAACCGCCGCCCGACCAAACTGAACGCCATTGCGTTCCTTCACATTCCGCCGCGTGGCGCTGa                                           >  2:330600/1‑294 (MQ=21)
                                                                                                                                                                                                                                      tGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGACCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGCCGCACTACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACAGAACGTCATTGCCTTCCATCAGATTCACCCGCGTGCTGCCGAt                                          >  2:128163/1‑294 (MQ=25)
                                                                                                                                                                                                                                       gCGACGCTGTCGCGTCTGATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATa                                         <  1:915003/294‑1 (MQ=255)
                                                                                                                                                                                                                                         gaggCTGTTGCGTTTTATCGGGCCTACAAATAGT‑CC‑GAACCGGAGGCCGGATAAGGCGTTTACGCCGCATCAGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATAcg                                       <  2:510248/291‑1 (MQ=255)
                                                                                                                                                                                                                                          aCGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCACATTCAACAGCgtgggtcgtctacgc                                      >  2:854816/1‑283 (MQ=255)
                                                                                                                                                                                                                                            gCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACgcgc                                    <  1:307763/294‑1 (MQ=255)
                                                                                                                                                                                                                                                 cgcgTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCgg                               <  1:746721/294‑1 (MQ=255)
                                                                                                                                                                                                                                                 agcgTCTTAGCGGGCGTACAAAGAGG‑CC‑GAACCGAGGGCGGGATAAGTGTGTGAGGGCGAATCCGGGAATGTGTGCATGATGCCTGATGGGACGCTGGCGCGTCGTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTGCACGCCGCAGCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCGTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCgg                               <  2:1078348/293‑1 (MQ=255)
                                                                                                                                                                                                                                                        tATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGCCCAAACTGAACGTCTTTGCGTTCCCTCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGACCAAcc                        >  1:556579/1‑294 (MQ=255)
                                                                                                                                                                                                                                                          tCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCa                      <  1:1010096/294‑1 (MQ=255)
                                                                                                                                                                                                                                                             tGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCg                   >  1:188962/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                 tACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATg               <  1:619495/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ccAAGTAGG‑CC‑GAACCGGAGCCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGa              <  2:304621/293‑1 (MQ=255)
                                                                                                                                                                                                                                                                     caTAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTGTACGCCGCATCCGGCAATTGGTGCAGGATGCCTGATGCGACGCTGGCGCGTCTGATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGAccc           <  1:611470/293‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aTAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCa          >  1:691045/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                         gT‑CC‑GGACCGGAGGCAGGTAAAGGCGTATAGGGCGAATCCGGCAATTAGTGCATGATGCCTGATGCGACGCTGGCGCGGCTTATCAGGCCTACATGGGGGGCGGGTCGGTAGACCGGATAAGGCGTTCACGCCGCCTCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAgcg       <  2:408872/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                             c‑GAACCGGATGCCGGATAAGGCGGTTAGGGCGCATCCGGAAATTGGGGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAGCGCGa    <  2:391300/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                             c‑GAACCGGAGGCCGGAGAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGGCTTATCAGGCCTACATTGGTGCCGGATCGGTAGAACGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAGCGCGa    <  1:204816/294‑1 (MQ=255)
                                                                                                                                                                                                                                                                               gcaCCGGAGGGCGGATAGGGCGTTTAGGCCGAATCCGGCAATTGGTGCATGATGCCTGATGCGACGGTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAGCGCGAt   <  2:145133/292‑1 (MQ=255)
                                                                                                                                                                                                                                                                                aaCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAGCGCGATc  >  1:369100/1‑294 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                    
CGCCTGTTTGGCGATGCGTTTGCATTTATCTGAGATATAAAAAAACCCGCCCCTGACAGGCGGGAAGAACGGCAACTAAACTGTTATTCAGTGGCATTTAGATCTATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGT‑CC‑GAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGACCGGATAAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTCGCAATACCCTGACCCAGACCGATACACATCGTCGCCAGACCAAACTGAACGTCTTTGCGTTCCATCAGATTCAGCAGCGTGGTGCTGATACGCGCACCGGAACAACCCAGCGGATGACCCAGCGCGATC  >  NZ_LN832404/4023669‑4024232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑