Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	15,571	T→C	100%	L43L (TTG→CTG)	EK12AG100_RS00080 →	IS4‑like element IS421 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	15,571	0	T	C	100.0%	79.5 / NA	27	L43L (TTG→CTG)	EK12AG100_RS00080	IS4‑like element IS421 family transposase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/11);  total (16/11)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTTTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCTGACGTGGCTCT  >  NZ_LN832404/15280‑15674                                                                                                                                                                                                                                                                                                     |                                                                                                         tGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCGTGGGTAAAAATAGGGTGGGTTGAAGATATGCGAGCACATGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTTACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTg                                                                                                        <  2:706649/294‑1 (MQ=255)     gacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAAACCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTAc                                                                                                    >  1:571657/1‑294 (MQ=255)         tgccccGCACACCACCCCAAAAGCCTGCCCTGGGGCAGGCCGGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCt                                                                                                <  2:309557/291‑1 (MQ=255)         tGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCCTATTGGTAAGCCCGACGAACTGGATACTTCGGCCCGTAATGCCCGGGCCCTAACCCGCCGCCCCGAAATTCGGTGTGCTGCCACTCTGCGACGtgt                                                                                                >  2:547268/1‑292 (MQ=21)                cTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACCTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTTCCGATGAATTACTCCCCCGATAACTGGTCAGCAATTCTGGTCCATTATGGTAAGCCCGAAGAACTGGATACTTCGGCACGCTATGCCGGGGCTCTAACCCGGCGCCGCGACATTCCGTATCCTGCAACCCTGCCACGGCTGGggccg                                                                                         >  2:250628/1‑292 (MQ=18)                  aaCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTTACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACACGCCGCCGCGAAATTCGTGATGCTGCAACTCTCCTACGTCTGGggctggc                                                                                       >  1:615952/1‑294 (MQ=255)                   aCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGggctggct                                                                                      >  1:658792/1‑294 (MQ=255)                          cAAAAGCCTGCCCGGTGGCAGGCCTGGGTAAAAATAGTGTGCGTTTAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGcc                                                                               <  2:214960/294‑1 (MQ=255)                           aaaaGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACCTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTTCAACTCTGCTCCGTCTGGGGCTGGCTTACGGccc                                                                              >  1:339099/1‑294 (MQ=255)                            aaaGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGAGATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGcccc                                                                             <  1:268882/294‑1 (MQ=255)                               gCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGGCCATATTTGTAAGCCCGAAGAACTGGCTACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCCACTCTGCTACGTCTGGGGCCTGCTGTCGGCCCCggg                                                                          >  2:225169/1‑294 (MQ=255)                                   gCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTTCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACTTACTTCCGGGGCCCTACCCCGCCGCCGCGCACTTCGTTCTTCTCAACCCCTGCTACGTCTTGGGCTGTCTTTCTGTCCCggggggg                                                                      >  2:166423/1‑293 (MQ=17)                                   gCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGa                                                                      >  1:818993/1‑294 (MQ=255)                                        tGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCCTAAACGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGACATTCTGGCCCATATTGGTTCGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGACGCTGCAACTCTGCTACGTCTGGGGCGTGCTGCCCGACCCGGGGGGCGGTCa                                                                 >  2:1077485/1‑294 (MQ=17)                                        tGGGCAGACCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCCCGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACTTCTGGGGCGGGCTTACGGCCACGGGGGGTAGTCa                                                                 >  1:633518/1‑294 (MQ=255)                                                 cTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGaa                                                       >  1:668025/1‑294 (MQ=255)                                                  tGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAg                                                      <  1:79559/294‑1 (MQ=255)                                                           aTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCAt                                             >  1:216382/1‑294 (MQ=255)                                                             aGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTCATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGCTGTCCTTAC‑GTGAAGTCACTGCATgg                                           >  1:540807/1‑294 (MQ=255)                                                                   cGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCCACTCTGCTATCTCTGGGGCTGGCTTACCGCCCCGGGGGGGTGGCCTTTCGGTGACGT‑ACTGCATGGGGTcct                                     >  2:364041/1‑292 (MQ=14)                                                                    gTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGCCTGGGGCTGGCTTACGGCCCCCGGGGGATGTCATTAC‑GGGAAGGCCCTGCATGGGATCacc                                    >  2:153978/1‑292 (MQ=255)                                                                       gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTcc                                 >  1:540820/1‑294 (MQ=255)                                                                       gAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCAAGATAACTGGTCAGCAATTCTGGCCCATCTTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGACCTGGGGGGATGTCATTAC‑GTGAAGTCCATGAATGGGCTACGCTcc                                 >  1:552342/1‑294 (MQ=14)                                                                         aggtttGCGCGCCCCTGTAATGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAAAGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGGGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCAt                               <  2:628832/289‑1 (MQ=255)                                                                         aGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCAt                               >  1:836211/1‑294 (MQ=255)                                                                             atGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCCCGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCCCGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTCCGTCTGGGGCTGGCCTTCGGCCCCGGGGGGATTGCCTTAC‑GTGAAGTCTACGCAGTGGCTCAGCTcctgtacg                           >  2:896793/1‑288 (MQ=14)                                                                                    cACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCGTTACGGTGCCAc                    >  1:434466/1‑294 (MQ=255)                                                                                    cACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCATGACGTTGCAAc                    <  1:23213/294‑1 (MQ=37)                                                                                    cACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAAACCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCATGACGTTGCAAc                    >  1:111151/1‑294 (MQ=37)                                                                                           aaaGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCt             >  1:791784/1‑294 (MQ=21)                                                                                           aaaGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCt             <  1:430411/294‑1 (MQ=21)                                                                                            aaGTGGCTGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCTg            <  1:636162/294‑1 (MQ=18)                                                                                              gtgtcagtgATCACTCCCGTAAGGGTGAACTTAAGGTGTTG‑GGTATTACGCCTGATATGATTGAACGTGCCGATGAATTACGCTCACGATAACTGGTCAGCAATTCTGGCCCATAGTGGTAAGCCCGAAGAACGGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCTGAc          <  2:33489/286‑1 (MQ=11)                                                                                                   ggggATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCTGACGTGGc     >  1:141061/1‑294 (MQ=17)                                                                                                     ggATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCT‑ACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCATGCCGTTGCAATATTATCTGACGTgcttct  >  1:313280/1‑289 (MQ=9)                                                                                                      gATCACTCCCCTAAGCGCAAATTTAAGG‑GTTGTGGTATTACGCCGGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTCTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCTGACGTGGctct  <  2:111151/294‑1 (MQ=9)                                                                                                                                                                                                                                                                                                     |                                                                                                         TGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGG‑GTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTCTAACCCGCCGCCGCGAAATTCGTGATGCTGCAACTTTGCTACGTCTGGGGCTGGCTTACGGCCCCGGGGGGATGTCATTAC‑GTGAAGTCACTGCATGGGCTCAGCTCCATGACGTTGCAACATTATCTGACGTGGCTCT  >  NZ_LN832404/15280‑15674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑