Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_LN832404	1,238,940	A→T	18.6%	E426D (GAA→GAT)	EK12AG100_RS06230 →	NADP‑dependent isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_LN832404	1,238,940	0	A	T	18.6%	172.8 / 34.4	70	E426D (GAA→GAT)	EK12AG100_RS06230	NADP‑dependent isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (31/26);  new base T (9/4);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.99e-01

CTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGC  >  NZ_LN832404/1238662‑1239231                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        ggtgactacatttctgacgccctggcagcgcaggttggcggtatcggtatcgcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgctg                                                                                                                                                                                                                                                                                          <  1:762405‑M1/139‑1 (MQ=255) ggtgactacatttctgacgccctggcagcgcaggttggcggtatcggtatcgcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgctg                                                                                                                                                                                                                                                                                          <  1:545454‑M1/139‑1 (MQ=255)      ctgcatgtcggacgccctggcagcgcaggttggcggtatcgggagcgccccttgtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATg                                                                                                                                                                                                                                                                                     >  2:398504‑M1/151‑294 (MQ=255)          atttctgacgcccgggtagcgcggtttggcgggatcggtatcgcccctggtgcaaacatcggtgacgaatgctccctgtttgaagccccccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTT‑Ca                                                                                                                                                                                                                                                                                >  1:509767‑M1/147‑294 (MQ=255)                   gccctggcagcgcaggttggcggtatcggtatcgcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActgctgaaatgttaacggtttgtt                                                                                                                                                                                                                                                                        <  2:264629‑M1/157‑12 (MQ=255)                     cctggcagcgcaggttggcggtatcggtatcgcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGCCTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGGAAACCGCAACCTATGCCTTCGAGCGTCTGATGGATTGCGCTAAACTGCTTAAATGGT‑CAGAGTTTGGTGa                                                                                                                                                                                                                                                                     <  2:164326‑M1/159‑1 (MQ=255)                     cctggcagcgcaggttggcggtatcggtatcgcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTT‑CAGAGTTTGGTGa                                                                                                                                                                                                                                                                     >  1:277341‑M1/136‑294 (MQ=255)                     cctggcagcgcaggttggcggtatcggtatcgcccctggtgcaaacatcggggacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTT‑CAGAGTTTGGTGa                                                                                                                                                                                                                                                                     >  2:92879‑M1/136‑294 (MQ=255)                           agcgcaggttggcggtatcggtatcgcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCCACGAGAAAACCGTAACCTATGACTTCGAGCGTCAGATGGATGGAGATAAAAGGCCGAAAAGTT‑CAGGGTtaggtgacgggtt                                                                                                                                                                                                                                                               <  2:496019‑M1/165‑7 (MQ=255)                           agcgcaggttggcggtatcggtatcgcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTT‑CAGAGTTTGGTGACGCGat                                                                                                                                                                                                                                                               <  1:507193‑M1/165‑1 (MQ=255)                            gcgcagggtggcgggaacggagtcacccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTT‑CAGAGTTTGGTGACGCGatc                                                                                                                                                                                                                                                              >  2:354976‑M1/129‑294 (MQ=255)                               cggggtggcggtttcggtatagcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTT‑CAGAGTTTGGTGACGCGatcatc                                                                                                                                                                                                                                                           >  2:179175‑M1/126‑294 (MQ=255)                                    tggcggtatcggtatcgcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCTAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGaaaa                                                                                                                                                                                                                                                      >  2:360323‑M1/121‑294 (MQ=255)                                             cggtatcgctccgggggcaaaatacggggacgaatgcgccctgtttgaagccacacacggtacgtcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGAGGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAAtc                                                                                                                                                                                                                                             >  2:282509‑M1/112‑294 (MQ=255)                                             cggtatcgcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTT‑CAGAGTTTGGTGACGCGATCATCGAAAACATGTAatg                                                                                                                                                                                                                                             >  1:602285‑M1/112‑293 (MQ=255)                                                 atcgcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGCTGGCGCTAAACTGCTGCAATGTT‑CAGAGTTTGGTGACGCGATCCTCGAAAatgtgtaaagccgt                                                                                                                                                                                                                                         <  2:441482‑M1/187‑7 (MQ=255)                                                    gcccctggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GAAATCAACGCGAAAACCGTAACATATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAAATGT‑AAGAGTTTGGAGAAGGGATCCacgaaaacatgttcagcggtagt                                                                                                                                                                                                                                      <  2:109375‑M1/190‑12 (MQ=255)                                                         tggtgcaaacatcggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTT‑CAGAGTTTGGTGACGCGATCATcgaaaacatgtaatgccgtagtttgtt                                                                                                                                                                                                                                 >  1:744917‑M1/100‑281 (MQ=255)                                                                     cggtgacgaatgcgccctgtttgaagccacccacggtactgctccgaaatatgccggtcaggacaaagtaaatccttgctctattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAAc                                                                                                                                                                                                                    >  1:957189‑M1/88‑294 (MQ=255)                                                                      ggtgacgaatgcgccctgtttgaagccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTACATATTGAAACg                                                                                                                                                                                                                   <  1:653752‑M1/208‑1 (MQ=255)                                                                                             agccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAAATGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTT‑CAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTG‑TTAAATTTATTAACGGGAGCG‑TAACGCTCCCGTTGttt                                                                                                                                                                                           >  1:757245‑M1/64‑294 (MQ=255)                                                                                               ccacccacggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGC‑GCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTT‑CAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTG‑TTAAATTTATTAACGGGAGCG‑TAACGCTCCCGTTgttttt                                                                                                                                                                                         <  1:901815‑M1/233‑2 (MQ=255)                                                                                                     acggtactgcgccgaaatatgccggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTAtg                                                                                                                                                                                   >  1:132463‑M1/56‑294 (MQ=255)                                                                                                                            ggtcaggacaaagtaaatcctggctctattatTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCg                                                                                                                                                            <  1:819451‑M1/262‑1 (MQ=255)                                                                                                                                                              tagtcatctaactaATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCa                                                                                                                         >  1:754227‑M1/15‑294 (MQ=255)                                                                                                                                                               agtcatctaactaATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACAAGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCTTATCTAGCATATTTATATGAA‑GCCCATTACTTAAGCCcaa                                                                                                                        <  2:68108‑M1/281‑2 (MQ=255)                                                                                                                                                                   atctaactaATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATgg                                                                                                                    <  1:236784‑M1/285‑1 (MQ=255)                                                                                                                                                                   atctaactaATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTAATTGAGcccataagg                                                                                                                    <  1:1055988‑M1/285‑4 (MQ=255)                                                                                                                                                                    tctaactaATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCAGTTGATTTATTTATGTGGATATTATTAATAGCATATCGAACATATTTATATGAA‑GCACAATACTTgaacccaataggg                                                                                                                   <  2:970374‑M1/286‑7 (MQ=255)                                                                                                                                                                       aactaATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCat                                                                                                                >  1:754026‑M1/6‑294 (MQ=255)                                                                                                                                                                            atgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTG‑TTGGTAAAGGTTAGGAAGGCGGCAACCAATGCAAAGACGTTAATTTATAATTTGGAACGCCGGTTGAAGGCCGCAAGGCTG‑TTAAATGTTCCGGGGTTTGGTGAAGCGATC‑TCGCAAAAATGGAATTCTTCCCAGTGTTTAATTATTGAAA‑GGGCCTATTAACACGCCCTTTGTTTATTTTATGTGACTATATTTAATACCTAATCGA‑CAATTTTTTATGAACGCCCATACTTTGATCCAAAAT‑GGCATtttttt                                                                                                          >  2:317719/1‑294 (MQ=255)                                                                                                                                                                             agaggtggCATATGGGTTGGACTGAAGCGGCTGAACTGATTGGTAAAGGTATGGAAGTC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATAttttt                                                                                                          >  2:1044617‑M1/9‑294 (MQ=255)                                                                                                                                                                              GCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTta                                                                                                         <  1:94937‑M1/294‑1 (MQ=255)                                                                                                                                                                                 GCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATaa                                                                                                      >  1:660096‑M1/1‑294 (MQ=255)                                                                                                                                                                                  CGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAAt                                                                                                     <  1:732973‑M1/294‑1 (MQ=255)                                                                                                                                                                                       TATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAAc                                                                                                <  2:61741‑M1/294‑1 (MQ=255)                                                                                                                                                                                       TATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAAc                                                                                                <  1:971240‑M1/294‑1 (MQ=255)                                                                                                                                                                                       TATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAAc                                                                                                >  1:461997‑M1/1‑294 (MQ=255)                                                                                                                                                                                       TATGGGTGGGACGGAGGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAAc                                                                                                >  2:431696‑M1/1‑294 (MQ=255)                                                                                                                                                                                           GGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACtatt                                                                                            >  1:480850‑M1/1‑294 (MQ=255)                                                                                                                                                                                            GTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACtatta                                                                                           <  1:633352‑M1/294‑1 (MQ=255)                                                                                                                                                                                             TTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACtattat                                                                                          >  1:711205‑M1/1‑294 (MQ=255)                                                                                                                                                                                             TTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACtattat                                                                                          >  2:239159‑M1/1‑294 (MQ=255)                                                                                                                                                                                               GGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGt                                                                                        <  1:927640‑M1/294‑1 (MQ=255)                                                                                                                                                                                                GACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTa                                                                                       <  1:728559‑M1/294‑1 (MQ=255)                                                                                                                                                                                                     aatgGGCTGACCTGATTGTTAAAGGAATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACat                                                                                  >  2:838170‑M1/5‑294 (MQ=255)                                                                                                                                                                                                           CTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACatttattt                                                                            <  1:1040498‑M1/294‑1 (MQ=255)                                                                                                                                                                                                           CTGACCTAATTTGTAAAGGTATGGAAGGC‑GCAAGCAATGCCAAGACCTTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCGGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACatttattt                                                                            >  2:286040‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                CTGATGGTTAAAGGTATGGAAAGC‑GCAATAAATGCCAAAACCGTAACTTATGACTTCGAACGGCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATTTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTAt                                                                       >  2:382387‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                 TGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGGAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTAtt                                                                      >  1:337942‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                 TGATGGTAAATGGCATGCACGGC‑GTAATCAAGGCCAAGACCGTAACTTATGACTTTGAACGTCTGATTGAAGGCGCTAATCTGCTGAAATGTT‑CAGAGTTGTGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTAtt                                                                      >  2:556010‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                   ATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTAGGACTTCGAACGGCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTAtttt                                                                    >  2:896443‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                    TTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTg                                                                   <  1:19815‑M1/294‑1 (MQ=255)                                                                                                                                                                                                                     TGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGc                                                                  >  2:210859‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                        TAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATTTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCttt                                                               >  1:972221‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                         AAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTtc                                                              <  1:508650‑M1/294‑1 (MQ=255)                                                                                                                                                                                                                         AAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTtc                                                              <  2:416019‑M1/294‑1 (MQ=255)                                                                                                                                                                                                                         AAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTtc                                                              >  1:994262‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                         AAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCACATTACTTGAGACCATATGGGCATCTTTTTATAATGAAACTATTATGTAAACATTTATTTGTTATTTTGatttt                                                              <  2:85876‑M1/294‑2 (MQ=255)                                                                                                                                                                                                                           AGGTATGGAAGGC‑GCAAGCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTtctc                                                            >  1:550609‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                            GGTATGGAAGGA‑GTAATCAATGACAAGACCGTAACTGAGGACGTAAAACATCTGATGGAAAGGGCTAAGCAGCTGAAATGAT‑AATAGTTTGGTGAAGCCATCATCGAAATCATGTAAGCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTcc                                                           >  2:634782‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                                 GGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTAAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAACCATTTATTTGTTATTTTGCTTTCTCCTggag                                                      <  2:68063‑M1/294‑1 (MQ=255)                                                                                                                                                                                                                                  GAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTggagg                                                     <  1:396580‑M1/294‑1 (MQ=255)                                                                                                                                                                                                                                   AAGGC‑GAAATCAAGGCCAAGACCTTAACTTATGACTTCGAACTGCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTggagga                                                    >  1:488168‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                                     GGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGaca                                                  <  1:78148‑M1/294‑1 (MQ=255)                                                                                                                                                                                                                                             TCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGa                                           >  2:712982‑M1/1‑294 (MQ=255)                                                                                                                                                                                                                                                  gCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATTCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCt                                      >  1:714626/1‑294 (MQ=255)                                                                                                                                                                                                                                                             aaCTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCCTATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTCTGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGACCAATCTTGACTGCTTTTAAGTaacc                           >  1:1079157/1‑292 (MQ=255)                                                                                                                                                                                                                                                                   tGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTAAACTCCATa                     <  1:970374/294‑1 (MQ=255)                                                                                                                                                                                                                                                                        tCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTTATTAAACTCCATAAATcc                >  1:584673/1‑294 (MQ=255)                                                                                                                                                                                                                                                                          gAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTTAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTGAATATTGACACGGTCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCtt              <  1:327010/294‑1 (MQ=255)                                                                                                                                                                                                                                                                          gAACGTCGGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CATAGTTTGGTGAAGCTATCAGCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAATACGCCCGTTGTGTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GTCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCtt              <  2:742600/294‑1 (MQ=255)                                                                                                                                                                                                                                                                           aaCGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑TAGAGGTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTTTTTTATTTATGTGGATATTATTAATAGCATATCGAGCAGATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCttg             <  2:698764/294‑1 (MQ=255)                                                                                                                                                                                                                                                                              gTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGACACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACCTTTATTTGTTATTTTGCTTTCTCCTGGAGGACCTTCTTGCCTGCTTTTGAGTAAACCCCATCAATCCtttttg          >  2:794572/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                cTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGaa        <  1:980617/294‑1 (MQ=255)                                                                                                                                                                                                                                                                                 tGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAACCATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAAt       >  1:382044/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                 tGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAAt       >  1:136082/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                  gATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATg      <  1:538913/294‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGc  >  2:54852/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                        CTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGC‑GCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTT‑CAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTG‑TTAAATATTGAAACGGGCGTA‑TAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAA‑GCCCATTACTTGAGCCCATATGGGCATATTTTTATAATGCAACTATTATGTAAACATTTATTTGTTATTTTGCTTTCTCCTGGAGGACACTCTTGACTGCTTTTGAGTAAACTCCATAAATCCTTGTTGAATGGTGC  >  NZ_LN832404/1238662‑1239231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑